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french Validation of the Screen for Cognitive Impairment in Psychiatry (SCIP-f)

Open Journal of Psychiatry 2016年第1期6卷 107-118页

作者： Smadar Valérie Tourjman Miriam H. Beauchamp Akram djouini Mathilde Neugot-Cerioli Charlotte Gagner Philippe Baruch Serge Beaulieu florence Chanut Andrée daigneault Robert-Paul Juster sonia Montmayeur Stéphane Potvin Scot Purdon Suzanne Renaud Evens Villenneuve Institut Universitaire en Santé Mentale de Montréal Montreal Canada Centre de Recherche de l’H?pital Ste-Justine Montreal Canada Institut Universitaire en Santé Mentale de Québec Quebec Canada douglas Mental Health University Institute Montreal Canada Centre Hospitalier Universitaire de Montréal Montreal Canada faculté de Pharmacie d’Angers Université Angers Angers France department of Psychiatry University of Alberta Edmonton Canada

Background: Measuring cognition in clinical practice is clearly essential to the appropriate characterisation of patients’ clinical status and to the development of a personalised care plan. The Screen for Cognitive Impairment in Psychiatry (SCIP) has been developed in order to provide a brief and accessible tool allowing the evaluation of cognitive function in psychiatric conditions. Objective: We present a validation of a french version of the SCIP. Method: Translation from English into french is carried out using the accepted back-translation method. Seventy-two healthy volunteers are characterised by demographic questionnaires and a neuropsychological battery. The french version of the SCIP is then administered on two separate occasions separated by at least a one-week interval. Results: High internal consistencies as well as strong correlations with comparable neuropsychological tests are obtained. A normalised Cronbach’s α = 0.66 is obtained. Conclusions: The french version of the SCIP (SCIP-f) yields results comparable to the English version. The SCIP represents an essential tool for the preliminary evaluation of cognition. Its characteristics, brevity and the lack of need for a technological platform, allow for its integration into clinical practice. further testing of SCIP-f in various psychiatric conditions will yield valuable information on its potential in clinical settings.

关键词： Screen for Cognitive Impairment in Psychiatry Cognitive deficits Neuropsychological Assessment Cognitive Screening Patient-Centered Approaches

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CTNS Molecular Genetics Profile in a Portuguese Cystinosis Population

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Open Journal of Genetics 2018年第4期8卷 91-100页

作者： filipa ferreira Ines Leal david Sousa Teresa Costa Conceicao Mota Ana Marta Gomes daniela Lopes Maria do Carmo Macario Isabel Tavares Helena Pinto Joao Paulo Oliveira Rita Magrico Celia Carmona sonia Ramos Raquel Neiva Ana Marcao Laura Vilarinho Newborn Screening Metabolism and Genetics UnitHuman Genetics DepartmentNational Institute of Health Dr Ricardo JorgePortoPortugal Centro Hospitalar Lisboa Norte Hospital de Santa MariaLisboaPortugal Centro Hospitalar Universitario do Porto PortoPortugal Centro Hospitalar Vila Nova de Gaia EspinhoPortugal Centro Hospitalar Universitario de Coimbra CHUCCoimbraPortugal Servico de Genetica Medica Centro Hospitalar de Sao JoaoPortoPortugal Unidade de Genetica Faculdade de Medicina da Universidade do PortoPortoPortugal I3S—Instituto de Investigacao e Inovacao em Saude PortoPortugal Servico de Nefrologia do Hospital Garcia de Orta AlmadaPortugal Research and development Unit Department of Human GeneticsNational Institute of Health Dr Ricardo JorgePortoPortugal

Background: Cystinosis is a multisystemic autosomal recessive deficiency of the lysosomal membrane transporter protein (cystinosin) caused by mutations in CTNS gene. Objective: This study summarizes the Portuguese experience in the diagnosis and management of patients with this rare disease over the past few years and reports recurrent mutations in the CTNS gene. Methods: Unrelated patients from different pediatric and adult hospitals all over Portugal with non-nephrotic proteinuria, hypercalciuria, hypokalemia impaired proximal reabsorption of amino acids, glycosuria and hypophosphatemia, suggestive of a fanconi syndrome and ocular problems, were studied. Intra-leukocyte cystine levels were determined and molecular analysis was performed, to determine the presence or absence of the 57-kb deletion in CTNS, followed by direct sequencing of the coding exons of CTNS. Results: from 1998 to 2017, twenty-one cystinotic patients were biochemically diagnosed. from the remaining seventeen (four deceased), eleven were studied for CTNS gene. five out of eleven patients were homozygous for the 57-kb deletion (10/22;45.5%), and other five were compound heterozygous for this variant (15/22;68.2%). The other mutations found were p.Q128X (c.721 C>T;2/22), p.S139f (c.755 C>T;4/22) and c.18-21delGACT (p.T7ffsX7;1/22). All of these seventeen cystinotic patients are in treatment. Approximately 84% are adults, 16% are young children, and 54.5% are kidney transplant recipient. Conclusions: The authors would like to emphasize the importance of first screening for the 57-kb deletion since it is very common in our population. This genetic study is the first in our country and it could be the basis for future genetic counseling in Portuguese population.

关键词： Cystinosis CTNS Gene Mutational Spectrum Kidney failure 57-kb deletion

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One-Step Synthesis and Characterization of a Nanocomposite Based on Carbon Nanotubes/Aluminum and Its Reinforcement Effect on the Metal Matrix

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材料科学与工程（中英文B版） 2015年第7期5卷 311-319页

作者： Marcos Allan Leite dos Reis Elizabeth M. S. Rodrigues Jordan del Nero sonia Simoes filomena Viana Manuel f. G. Vieira T. f. Vieira faculty of Exact Science and Technology Federal University of Para Abaetetuba PA 68440-000 Brazil Institute of Exact Science Federal University of Para Belem PA 68440-000 Brazil CEMUC Faculty of Engineering of the University of Porto (FEUP) University of Porto Rua Dr. Roberto Frias 4200-465 PortoPortugal CEMUC Department of Mechanical Engineering Universidade of Coimbra Pinhal de Marrocos 3030-788 Coimbra Portugal

The nanocomposite based on carbon nanotubes/aluminum (CNT/Al) was one-step prepared by the dC arc discharge method under an argon/acetone mixed atmosphere. Synthesis was performed by arc plasma on a pure graphite rod, filled by aluminum powder as an anode and aluminum plate as a cathode. discharge conditions of 85 A and 20 V were used at a pressure range of 375 to 750 Torr. The CNT/Al was characterized by Scanning Electron Microscopy with Energy dispersive Spectroscopy; Transmission Electron Microscopy; Laser diffraction Particle Size Analysis; X-Ray diffraction; Raman Spectroscopy; Thermogravimetric Analysis; and, its reinforcement effect of the CNTs on the aluminum matrix was measured by Vickers microhardness test. This nanocomposite shows agglomerates of multiwalled carbon nanotubes filled with Al4C3 and blended with aluminum particles; moreover, in the nanocomposites was found a hardness increase of 40% for nanocomposites with 1.0 wt.% of CNT/Al.

关键词：多壁碳纳米管纳米复合材料一步合成铝板强化作用金属基体扫描电子显微镜透射电子显微镜

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Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model

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World Journal of Hepatology 2016年第21期8卷 902-914页

作者： Sylvie Lagaye sonia Brun Jesintha Gaston Hong Shen Ruzena Stranska Claire Camus Clarisse dubray Géraldine Rousseau Pierre-Philippe Massault Jerome Courcambeck firas Bassisi Philippe Halfon Stanislas Pol Institut Pasteur INSERM Institut Cochin INSERM U1016CNRS UMR8104Universite Paris Descartes(UMR S1016) Genoscience Pharma KU Leuven Rega Institute Laboratoire Alphabio APHP Groupe Hospitalier La Pitie Salpe-triereService de Chirurgie digestive et Hepato bilio pancreatique APHP Groupe Hospitalier CochinService de Chirurgie digestiveHepato-biliaire et Endocrinienne Universite Paris descartes APHP Groupe Hospitalier CochinUnite d’Hepatologie Institut Pasteur Departement de Recherche TranslationnelleINSERM

AIM: To evaluate the antiviral potency of a new antihepatitis C virus(HCV) antiviral agent targeting the cellular autophagy machinery. METHOdS: Non-infected liver slices, obtained from human liver resection and cut in 350 μm-thick slices(2.7 × 106 cells per slice) were infected with cell culture-grown HCV Con1b/C3 supernatant(multiplicity of infection = 0.1) cultivated for up to ten days. HCV infected slices were treated at day 4 post-infection with GNS-396 for 6 d at different concentrations. HCV replication was evaluated by strand-specific real-time quantitative reverse transcription- polymerase chain reaction. The infectivity titers of supernatants were evaluated by foci formation upon inoculation into naive Huh-7.5.1 cells. The cytotoxic effect of the drugs was evaluated by lactate dehydrogenase leakage assays. RESULTS: The antiviral efficacy of a new antiviral drug, GNS-396, an autophagy inhibitor, on HCV infection of adult human liver slices was evidenced in a dosedependent manner. At day 6 post-treatment, GNS-396 EC50 was 158 nmol/L without cytotoxic effect(compared to hydroxychloroquine EC50 = 1.17 μmol/L).CONCLUSION: Our results demonstrated that our ex vivo model is efficient for evaluation the potency of autophagy inhibitors, in particular a new quinoline derivative GNS-396 as antiviral could inhibit HCV infection in a dosedependent manner without cytotoxic effect.

关键词： Host antiviral therapy Hepatitis C virus Tissue culture Autophagy Quinoline derivative

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dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-domínguez Juan Carlos Crespo-Rivas Alberto fernández-Villar Alexandre Pérez-González Luis fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María dolores Avilés Maribel Asensio Bosco Barón-franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan delgado Antonio domínguez-Petit Alejandro deniz Reginal dusseck-Brutus Ana Escoresca-Ortega fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen ferrándiz-Millón Marta ferrer Teresa ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis f.López-Cortés Rafael Luque-Márquez daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María dolores Navarro-Amuedo dolores Nieto-Martín francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(dCs)play a key role in the defense against viral infections,for instance plasmacytoid dCs(pdCs),have the capacity to produce vast amounts of interferon-alpha(IfN-α).In COVId-19 there is a deficit in dC numbers and IfN-αproduction,which has been associated with disease *** this work,we described that in addition to the dC deficiency,several dC activation and homing markers were altered in acute COVId-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of Cd1c+myeloid dCs and pdCs seven months after SARS-CoV-2 ***,the expression of dC markers such as Cd86 and Cd4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IdO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVId-19.

关键词： SARS-CoV-2 COVId-19 dendritic cell Long-COVId

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Synchronous carcinoma of head and neck: 2 cases report

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World Journal of Otorhinolaryngology 2015年第2期5卷 74-77页

作者： Ali Mardassi Nabil Mathlouthi Nawel Hlila Chiraz Halouani Sameh Mezri Cyrine Zgolli Ghassen Chebbi Rania Ben Mhamed Khemaies Akkari sonia Benzarti Ear Nose and Throat department the Military Hospital Tunis

Multiple primary cancers are becoming an increasingly frequent situation and are often the source of many diagnostic and therapeutic difficulties. We report the case of two patients diagnosed with head and neck synchronous carcinomas. The first case is a 33-yearold man with a history of a keratinizing squamous cell carcinoma of the eye lid and who was operated 4 mo later from a mucoepidermoid carcinoma of thesubmandibular salivary gland. The second case is a 71-year-old woman who underwent a total parotidectomy for a mucoepidermod carcinoma of the left parotid gland and who consulted 2 mo later for epistaxis. The explorations concluded to a squamous cell carcinoma of the nasopharynx. The patient had a complementary radiotherapy. No local neither distant recurrence of the two tumors has been detected after a follow-up of 36 mo in the first case and 24 mo in the second one.

关键词： Carcinoma Submandibular gland Parotid gland Nasopharynx Radiotherapy

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The Thermal Properties of Natural Pozzolan and Energy Efficiency in Buildings

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Journal of Control Science and Engineering 2014年第2期2卷 120-127页

作者： Hamadache Miloud M. Mouli N. Bouhamou O. Chaib A. S. Benosman f. dif Materials Laboratory Department of Civil Engineering National Polytechnic School BP 1524 El Mnaouer Oran 31000 Algeria Laboratory Construction Transportation and Protecting the Environment University of Mostaganem Mostaganem 27000Algeria facultyof Exact Sciences and Applied Faculty of Science Laboratory of Polymer Chemistry University of Oran Es-sOnia Oran31000 Algeria

The objective of this work is the study of recoverability of materials. It consists in studying the effectiveness of the addition of natural pozzolan on the thermal properties of the mortar. To do this, measuring instruments were installed to quantify the overall indoor environment and in particular the thermal environment. Given that the thermal environment is characterized by the physical quantities: the temperature of air, wall temperature and the air humidity. This allows gauging the level of the energy consumption and thermal behavior of the envelope of structure in order to verify the conformity of the housing to the requirements of thermal comfort. for this, we measured the thermal conductivity of mortars containing different contents of pozzolan (10%, 20%, 30%). So, the results showed the beneficial effect of pozzolan as thermal insulator by comparing with other insulation materials.

关键词：控制学控制论学习机理论最优控制

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2019年全球、地区和国家5岁以下儿童呼吸道合胞病毒引发急性下呼吸道感染疾病负担研究的系统分析

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中国病毒病杂志 2023年第2期13卷 81-95页

作者： You Li Xin Wang dianna M.Blau Mauricio T.Caballero daniel R.feikin Christopher J.Gill Shabir A.Madhi Saad B.Omer Eric A.f Simoes Harry Campbell Ana Bermejo Pariente darmaa Bardach Quique Bassat Jean-Sebastien Casalegno Giorgi Chakhunashvili Nigel Crawford daria danilenko Lien Anh Ha do Marcela Echavarria Angela Gentile Aubree Gordon Terho Heikkinen QSue Huang Sophie Jullien Anand Krishnan Eduardo Luis Lopez Josko Markic Ainara Mira-Iglesias Hannah CMoore Jocelyn Moyes Lawrence Mwananyanda dJames Nokes faseeha Noordeen Evangeline Obodai Nandhini Palani Candice Romero Vahid Salimi Ashish Satav Euri Seo Zakhar Shchomak Rosalyn Singleton Kirill Stolyarov sonia K Stoszek Anne von Gottberg danielle Wurzel Lay-Myint Yoshida Chee fu Yung Heather J Zar Harish Nair 张甜甜从兵兵邓舒予不详南京医科大学公共卫生学院江苏南京211166

背景呼吸道合胞病毒(respiratory syncytial virus,RSV)是引起婴幼儿急性下呼吸道感染最常见的病原体。此前估计,2015年全球60月龄以下儿童共发生3 310万次RSV相关急性下呼吸道感染,导致118 200人死亡。此后,几项社区监测研究获得了对RS... 详细信息

背景呼吸道合胞病毒(respiratory syncytial virus,RSV)是引起婴幼儿急性下呼吸道感染最常见的病原体。此前估计,2015年全球60月龄以下儿童共发生3 310万次RSV相关急性下呼吸道感染,导致118 200人死亡。此后,几项社区监测研究获得了对RSV相关社区死亡的更精确估计。本文旨在更新2019年全球、地区和国家层面60月龄以下儿童RSV相关急性下呼吸道感染的发病率和死亡率,重点关注总体死亡率和在研RSV免疫制剂针对的更窄的婴儿年龄组。方法检索获得MEdLINE、Embase、Global Health、CINAHL、Web of Science、LILACS、OpenGrey、中国知网、万方和维普数据库2017—2020年发表文献中的新数据,并纳入RSV GEN合作者提供的未发表数据,以扩展全球RSV疾病负担数据集。纳入的研究符合以下条件:报告了社区中或需要住院治疗的60月龄以下儿童RSV原发性急性下呼吸道感染的数据;报告了至少连续12个月的数据,但院内病死率或RSV季节性定义明确的地区除外;报告了RSV相关急性下呼吸道感染的发病率、住院率、院内病死率或急性下呼吸道感染住院的RSV阳性比例。如果病例定义不明或应用不一致、RSV感染未经实验室确认或仅基于血清学检测、报告的急性下呼吸道感染病例少于50例,则排除该研究。本研究应用广义线性混合效应模型(generalized linear mixed-effects model,GLMM)估计了2019年全球和地区(按国家发展状况和世界银行收入分类)RSV相关急性下呼吸道感染的发病率、住院率和院内病死率;通过基于危险因素的模型估计了国家层面RSV相关急性下呼吸道感染的发病率;通过GLMM开发了新模型,纳入最新的RSV社区死亡率数据,用于估计RSV的总体死亡率。本综述已在PROSPERO注册(CRd42021252400)。结果除了先前综述中纳入的317项研究外,还纳入了113项符合条件的新研究以及51项未发表的研究,共计481项研究。据估计,2019年全球60月龄以下儿童中,共发生3 300万(置信区间为2 540万~4 460万)次RSV相关急性下呼吸道感染,导致360万(290万~460万)例住院、26 300(15 100~49 100)例院内死亡以及101 400(84 500~125 200)例可归因于RSV的总死亡数。在0~6个月的婴儿中,共发生660万(460万~970万)次RSV相关急性下呼吸道感染,导致140万(100万~200万)例住院、13 300(6 800~28 100)例院内死亡以及45 700(38 400~55 900)例可归因于RSV的总死亡数。60月龄以下和28 d~6月龄儿童的死亡中分别有2.0%(置信区间为1.6%~2.4%)和3.6%(3.0%~4.4%)归因于RSV。在各年龄组中,95%以上的RSV相关急性下呼吸道感染事件和97%以上的可归因于RSV的死亡事件都发生在中低收入国家。结论 RSV显著增加了全球60月龄以下儿童的发病和死亡负担,尤其是在出生后前6个月和中低收入国家。本研究强调了全球RSV疾病的总体死亡率负担,60月龄以下儿童中,每50例死亡就有1例可归因于RSV;28 d至6个月大的婴儿中,每28例死亡就有1例可归因于RSV。每1例RSV相关急性下呼吸道感染院内死亡,对应社区中约3例可归因于RSV的死亡。对6月龄内婴儿提供保护的RSV被动免疫计划有望对减轻RSV疾病负担,但实施计划时,仍需要更多数据来了解RSV疾病负担高峰可能向大龄转移的影响。

关键词：呼吸道合胞病毒下呼吸道感染儿童疾病负担住院发病率死亡率病死率模型估计系统分析

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