检索结果-南通市图书馆

IL-6通过激活JAK2/STAT3信号通路增强小鼠肺泡巨噬细胞的吞噬功能

在线全文

学校读者我要写书评

暂无评论

细胞与分子免疫学杂志 2024年第1期40卷 13-18页

作者：华梦晴高培宇方芳苏浩宇宋传旺蚌埠医学院检验医学院免疫学教研室安徽蚌埠233030 蚌埠医学院慢性疾病免疫学基础与临床安徽省重点实验室安徽蚌埠233030

目的探讨白细胞介素6(IL-6)对MH-S小鼠肺泡巨噬细胞吞噬功能的影响及其相关机制。方法脂多糖(LPS)经气道滴入激发构建小鼠急性肺损伤(ALI)模型,ELISA检测支气管肺泡灌洗液(BALF)中IL-6的含量。体外培养MH-S细胞,在信号转导子与转录激... 详细信息

目的探讨白细胞介素6(IL-6)对MH-S小鼠肺泡巨噬细胞吞噬功能的影响及其相关机制。方法脂多糖(LPS)经气道滴入激发构建小鼠急性肺损伤(ALI)模型,ELISA检测支气管肺泡灌洗液(BALF)中IL-6的含量。体外培养MH-S细胞,在信号转导子与转录激活子3 (STAT3)抑制剂Stattic(5μmol/L)存在与否的情况下,再加入IL-6(10 ng/mL~500 ng/mL)刺激6 h,加入荧光微球孵育2 h后,采用流式细胞术检测MH-S细胞吞噬荧光微球情况;Western blot法检测磷酸化的Janus激酶2(p-JAK2)、磷酸化的STAT3(p-STAT3)、肌动蛋白相关蛋白2(Arp2)、纤维型肌动蛋白(F-actin)的表达水平。结果鼻腔滴入LPS后,小鼠BALF中IL-6含量显著升高。随着IL-6刺激剂量的增加,MH-S细胞吞噬荧光微球的作用增强,Arp2、 F-actin蛋白的表达水平升高。100 ng/mL IL-6刺激MH-S细胞后,p-JAK2和p-STAT3蛋白的表达水平升高。阻断MH-S细胞STAT3信号后,IL-6促进细胞吞噬的效应完全消失,IL-6诱导的Arp2、 F-actin蛋白表达增加被抑制。结论 IL-6通过激活JAK2/STAT3信号通路促进MH-S细胞Arp2、 F-actin蛋白的表达增强吞噬功能。

关键词：白细胞介素6(IL-6) MH-S细胞吞噬功能 Janus激酶2(JAK2) 信号转导子与转录激活子3(STAT3) 肺泡巨噬细胞

酪酸梭菌二联活菌散配合蓝光照射治疗对高胆红素血症新生儿血清胆红素、炎症指标水平的影响

在线全文

学校读者我要写书评

暂无评论

反射疗法与康复医学 2024年第4期5卷 33-35+39页

作者：宋相菊姚金秀聊城市东昌府区妇幼保健院新生儿科聊城市东昌府区中医院儿科

目的探究酪酸梭菌二联活菌散配合蓝光照射治疗对高胆红素血症新生儿血清胆红素、炎症指标水平的影响。方法选取2022年8月—2023年8月聊城市东昌府区妇幼保健院收治的90例高胆红素血症新生儿为研究对象，按随机数字表法将其分为对照组... 详细信息

目的探究酪酸梭菌二联活菌散配合蓝光照射治疗对高胆红素血症新生儿血清胆红素、炎症指标水平的影响。方法选取2022年8月—2023年8月聊城市东昌府区妇幼保健院收治的90例高胆红素血症新生儿为研究对象，按随机数字表法将其分为对照组与观察组，各45例。对照组采用蓝光照射治疗，观察组在对照组基础上加用酪酸梭菌二联活菌散治疗。比较两组患者的临床疗效、血清胆红素水平、炎症指标水平及不良反应发生情况。结果观察组治疗总有效率为93.33%，高于对照组的77.78%。治疗后，观察组总胆红素水平为（143.16±14.04）μmol/L，直接胆红素水平为（14.42±2.15）μmol/L，间接胆红素水平为（164.03±13.54）μmol/L，降钙素水平为（0.16±0.04）μg/L,C反应蛋白水平为（2.42±1.17）mg/L，白细胞介素-6水平为（11.03±1.34）ng/L，均低于对照组的（225.40±17.35）μmol/L、（18.74±2.25）μmol/L、（215.49±18.85）μmol/L、（0.20±0.05）μg/L、（5.74±1.25）mg/L、（15.49±1.35）ng/L，组间差异有统计学意义（P0.05）。结论酪酸梭菌二联活菌散配合蓝光照射治疗可显著降低高胆红素血症新生儿血清胆红素水平安全性较高。

关键词：高胆红素血症新生儿酪酸梭菌二联活菌散蓝光照射血清胆红素

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

湖光岩玛珥湖沉积物雨水芳烃化合物组成研究

环境科学与技术 2024年第S1期47卷 21-27页

作者：马雪梅周迅高苑罗涛王遥平宋之光广东海洋大学化学与环境学院广东湛江524000

该文利用气相色谱-质谱联用仪,对大气降水、湖光岩玛珥湖表层湖水和沉积物中16种优控多环芳烃(PAHs)化合物进行研究。结果表明:(1)大气降水和表层湖水中检出15种优控PAHs,总浓度分别为244.78 ng/L和117.31 ng/L;沉积物中检出16种优控PA... 详细信息

该文利用气相色谱-质谱联用仪,对大气降水、湖光岩玛珥湖表层湖水和沉积物中16种优控多环芳烃(PAHs)化合物进行研究。结果表明:(1)大气降水和表层湖水中检出15种优控PAHs,总浓度分别为244.78 ng/L和117.31 ng/L;沉积物中检出16种优控PAHs,总浓度为304.42 ng/g。大气降水、表层湖水、沉积物中2~3环PAHs占比呈顺序降低,4~6环PAHs占比呈顺序递增。(2)大气降水中Chr、BbF、BkF、BaP、IcdP致癌单体多环芳烃含量超过标准规定限值,BaA略低于标准规定限值;湖光岩玛珥湖表层湖水中的BaA、BbF、BkF、BaP等致癌性多环芳烃化合物含量低于标准规定的限值,Chr和IcdP含量略高于标准规定的限值。(3)湖光岩玛珥湖沉积物中Nap、Acy、Ace、Flu、Phe、Ant、Fla、Pyr、BaA、Chr、BaP、DahA化合物含量均远低于沉积物质量基准值ERL,无生态风险。而尚无规定安全值的BbF、BkF、InP和BghiP的生态风险需要进一步关注。总体上湖光岩玛珥湖表层湖水和沉积物PAHs生态风险低。

关键词：表层湖水沉积物大气降水多环芳烃化合物湖光岩玛珥湖

Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第1期11卷 61-68页

作者： XueLi Yun-Yanzh.ng Qiwang song-BinFu LaboratoryofMedicalGenetics HarbinMedicalUniversityHarbin150086HeilongjiangProvinceChina ThirdAffiliatedHospitalofHarbinMedicalUniversity Harbin150086HeilongjiangProvinceChina

AIM: To investigate the association between endogenous gene expression and growth regulation including proliferation and apoptosis induced by transforming growth factor-pi (TGF-βl) in human gastric cancer (GC) cells. METHODS: Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the main components of the TGF-β1/Smads signal pathway in human poorly differentiated GC cell line BGC-823. Localization of Smad proteins was also determined using immunofluorescence. Then, the BGC-823 cells were cultured in the presence or absence of TGF-β1 (10 ng/mL) for 24 and 48 h, and the effects of TGF-β1 on proliferation and apoptosis were measured by cell growth curve and flow cytometry (FCM) analysis. The ultrastructural features of BGC-823 cells with or without TGF-β1 treatment were observed under transmission electron microscope. The apoptotic cells were visualized by means of the terminal deoxynucleotidyl transferase (TdT)-mediated dTUP in situ nick end-labeling (TUNEL) method. Meanwhile, the expression levels of endogenous p15, p21 and Smad7 mRNA and the corresponding proteins in the cells were detected at 1, 2 and 3 h after culture in the presence or absence of TGF-β1 (10 ng/mL) by semi-quantitative RT-PCR and Western blot, respectively. RESULTS: The TGF-β1/Smad signaling was found to be intact and functional in BGC-823 cells. The growth curve revealed the most evident inhibition of cell proliferation by TGF-β1 at 48 h, and FCM assay showed G1 arrest accompanied with apoptosis induced by TGF-β1. The typical morphological changes of apoptosis were observed in cells exposed to TGF-β1. The apoptosis index (AI) in TGF-β1-treated cells was significantly higher than that in the untreated controls (10.7±1.3% vs 0.32±0.06%, P＜0.01). The levels of p15, p21 and Smad7mRNA and corresponding proteins in cells were significantly up-regulated at 1 h, but gradually returned to basal levels at 3 h following TGF-βl (10 ng/mL) treatment. CONCLUSION: TGF-β1 affects both

关键词： Gastric cancer Transforming growth factor-β1 Apoptosis Gene expression

Cu-Catalyzed Chemoselective Reduction of N-Heteroaromatics with NH_(3)·BH_(3) in Aqueous Solution

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2021年第9期39卷 2504-2508页

作者： Chao Gao Qingqing Xuan Qiuling song Institute of Next Generation Matter Transformation College of Chemical Engineering and College of Material Sciences Engineering at Huaqiao University668 Jimei BoulevardXiamenFujian 361021China Key Laboratory of Molecule Synthesis and Function Discovery Fujian Province University College of Chemistry at Fuzhou University FuzhouFujian 350108China State Key Laboratory of Organometallic Chemistry and Key Laboratory of Organofluorine Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

An efficient catalytic system was successfully developed on reduction of A/-heteroaromatics with H3N-BH3 as hydrogen source in CuSO4 solution,featuri ng excel I e nt chemoselectivity as well as very broad functional group tolera *** challe nging substrates,such as OH-,NH_(2)^(-), Cl^(-),Br^(-),etc.,contained quinolines,quinoxalines,1,5-naphthyridines and quinazolines were all reduced *** nistic studies suggested that[Cu-H]in termediate might be gen erated from NH_(3)·BH_(3)/which was believed to form with H3N-BH3 in CuSO_(4) solution.

关键词： Nitrogen heterocycles NH_(3)·BH_(3) Hydrogen transfer [Cu-H]in termediate Chemoselectivity

一次性根管治疗与多次法根管治疗在牙体牙髓病患者中的疗效观察

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际医药卫生导报 2024年第7期30卷 1111-1116页

作者：卢健王亚军宋杰聊城市第三人民医院口腔科聊城252000

目的分析一次性根管治疗与多次法根管治疗在牙体牙髓病中的疗效。方法选取2020年3月至2023年3月在聊城市第三人民医院接受治疗的80例牙体牙髓病患者进行随机对照试验,按照随机数字表法分为观察组和对照组,各40例。对照组男21例、女19例... 详细信息

目的分析一次性根管治疗与多次法根管治疗在牙体牙髓病中的疗效。方法选取2020年3月至2023年3月在聊城市第三人民医院接受治疗的80例牙体牙髓病患者进行随机对照试验,按照随机数字表法分为观察组和对照组,各40例。对照组男21例、女19例,年龄(40.37±8.30)岁,病程(8.60±3.15)个月,接受多次法根管治疗;观察组男20例、女20例,年龄(40.22±8.45)岁,病程(8.55±3.20)个月,接受一次性根管治疗。对比两组患者治疗后临床疗效、疼痛程度[运用视觉模拟评分量表(VAS)评估]、使用镇痛药次数、治疗前及治疗后2周炎症因子[白细胞介素-6(IL-6)、白细胞介素-2(IL-2)、高敏C-反应蛋(hs-CRP)、血清骨保护素、可溶性核因子κB受体激活因子配体(RANKL)]、牙齿咀嚼功能(牙齿咬合力、出血指数、牙龈指数)、并发症发生情况。统计学方法采用t检验、χ^(2)检验。结果观察组治疗后总有效率为92.50%(37/40),高于对照组的72.50%(29/40),差异有统计学意义(χ^(2)=5.541,P=0.019)。观察组治疗后VAS评分、镇痛药使用率均低于对照组[(2.64±0.58)分比(3.24±0.67)分、0比10.00%(4/40)],差异均有统计学意义(t=4.282,χ^(2)=4.211;均P0.05);治疗后2周,观察组IL-6、IL-2、hs-CRP、RANKL均低于对照组[(1.16±0.34)ng/L比(1.34±0.35)ng/L、(0.29±0.04)ng/L比(0.33±0.06)ng/L、(1.14±0.11)mg/L比(1.65±0.22)mg/L、(0.35±0.06)ng/L比(0.39±0.08)ng/L],血清骨保护素高于对照组[(0.43±0.08)µg/L比(0.36±0.07)µg/L],差异均有统计学意义(t=2.233、3.508、13.114、2.530、4.165,均P0.05);治疗后2周,观察组出血指数、牙龈指数均低于对照组[(0.48±0.10)分比(0.90±0.19)分、(0.47±0.09)分比(0.76±0.15)分],牙齿咬合力高于对照组[(140.18±11.85)lbs比(116.75±9.97)lbs],差异均有统计学意义(t=12.372、10.485、9.569,均P<0.05)。观察组治疗后并发症发生率为5.00%(2/40),低于对照组的22.50%(9/40),差异有统计学意义(χ^(2)=5.165,P=0.023)。结论一次性根管治疗相较于多次法根管治疗效果较好,能有效减少牙体牙髓病患者疼痛,降低炎症水平及并发症发生率。

关键词：根管治疗术一次性多次法牙体牙髓病疼痛程度炎症因子

First principles study of behavior of helium at Fe(110)-graphene interface

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2021年第4期30卷 432-437页

作者： Yan-Mei Jing Shao-song Huang Key Laboratory of Material Modification by Laser Ion and Electron Beams(Dalian University of Technology)Ministry of EducationDalian 116024China

Recently,metal-graphene nanocomposite system has aroused much interest due to its radiation tolerance ***,the related atomic mechanism for the metal-graphene interface is still ***,stainless steels with Fe as main matrix are widely used in nuclear ***,in this study,the atomic behaviors of point defects and helium(He) atoms at the Fe(110)-graphene interface are investigated systematically by first principles *** results indicate that graphene interacts strongly with the Fe(110) *** comparison with those of the original graphene and bulk Fe,the formation energy values of C vacancies and Fe point defects decrease significantly for Fe(110)-***,as He atoms have a high migration barrier and large binding energy at the interface,they are trapped at the interface once they enter into *** theoretical results suggest that the Fe(110)-graphene interface acts as a strong sink that traps defects,suggesting the potential usage of steel-graphene with multiply interface structures for tolerating the radiation damage.

关键词： Fe(110)-graphene helium interface first principles calculations

Crystal phase engineering on photocatalytic materials for energy and environmental applications

在线全文

学校读者我要写书评

暂无评论

Nano Research 2019年第9期12卷 2031-2054页

作者： song Bai Chao Gao Jingxiang Low Yujie Xiong Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Life SciencesInstitute of Physical and ChemistryZhejiang Normal UniversityJinhua 321004China Hefei National Laboratory far Physical Sciences at the Microscale iChEM (Collaborative Innovation Center of Chemistry for Energy Materials)School oj Chemistry and Materials ScienceUniversity of Science and Technology of ChinaHefei 230026China

Crystal phase engineering on photocatalytic materials is a subfield of photocatalysis with intensive research,which has been proven as a:versatile approach to maneuver their performance for applications in energy-and environment-related *** this article,the state-of-the-art progress on phase-e ngin eered photocatalytic materials is ***,we discuss the phase engin eeri ng on pristi ne semic on ductor photocatalysts,in which the phase-dependent light absorption,charge transfer and separation,and surface reaction behaviors in photocatalytic processes are summarized,*** on the elucidated mechanisms,the implementation of phase junctions in photocatalytic reactions is then *** a focus,we highlight the rational design of phase junctions toward steering the charge kinetics for enhanced photocatalytic and photoelectrocatalytic ***,the crystal phase engineering on semiconductor-based hybrid photocatalysts is also in troduced,which un derli nes the importa nee of choosi ng a suitable phase for semic on ductor comp orients and co-catalysts as well as the synergism of differe nt semico nductor phases for improved photocatalytic performa *** ally,the challe nges and perspectives in this research field are *** this review,particular emphasis is placed on establishing a linkage between crystal phase and photocatalytic activity to develop a structure-activity *** on the guide,a framework is suggested for future research on the rational phase design of photocatalysts for improved performance in energy and environmental applications.

关键词： photocatalysis crystal phase charge kinetics architectural design energy environment

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

低水胶比湿喷混凝土的可喷性能研究

新型建筑材料 2018年第6期45卷 1-4,10页

作者：王巧王祖琦宋普涛周永祥杨长辉中国建筑科学研究院有限公司北京100013 重庆大学材料科学与工程学院重庆400045 建筑安全与环境国家重点实验室北京100013

喷射混凝土(湿喷法)通常以C30及以下强度等级为主,难以满足某些修补及隧道工程对高强喷射混凝土的需求。高强混凝土具有水胶比低、粘度大等特点,采用喷射施工的难度更大。研究了低水胶比混凝土的净浆流变性能、可输送性能,并采用小型湿... 详细信息

喷射混凝土(湿喷法)通常以C30及以下强度等级为主,难以满足某些修补及隧道工程对高强喷射混凝土的需求。高强混凝土具有水胶比低、粘度大等特点,采用喷射施工的难度更大。研究了低水胶比混凝土的净浆流变性能、可输送性能,并采用小型湿喷机验证了混凝土的可喷射性能。结果表明:新拌混凝土的可变形能力值Γ越接近7,其可输送性能越理想;一次喷射厚度随着拌合物屈服应力值τ的增大而增大,但塑性粘度η与喷射厚度没有明显的关系;C50混凝土中掺入82 kg/m^3降粘增强剂可明显提高湿喷混凝土的可喷射性,回弹率降至6.5%,一次喷射厚度为160 mm。

关键词：低水胶比湿喷混凝土可喷性回弹率