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Journal of Integrative Agriculture 2018年第11期17卷 2458-2466页

作者： song Xiao-xia ZHAO yan song chun-yan LI Chuan-hua CHEN Ming-jie HUANG Jian-chun TAN Qi Institute of Edible Fungi Shanghai Academy of Agricultural Sciences

China is currently the world＇s leading producer of Lentinula edodes and owns many cultivated strains of this species. This study was performed in order to investigate intergenic spacer I （IGS1） polymorphism and classification among 49 popular cultivated strains. The great majority of the 49 strains possessed two different IGS1 sequences, with distinct lengths and homologies. Based on the length and homology of the IGS1 sequences of the 49 strains, the strains were classified into two groups： A and B. Group A was subdivided into six subgroups. Forty-seven strains were homozygous or heterozygous among these six subgroups in group A, Cr01 was heterozygous between A and B, and Guangxiang 9 was homozygous in group B. An IGS1 polymorphism map of each cultivated L. edodes strain is reported for the first time and could be used as a marker for the initial classification and management of cultivated L. edodes strains in China.

关键词： Lentinula edodes strain intergenic spacer polymorphism genotype

Tensor Coupling Effects on Spin Symmetry in the Anti-Lambda Spectrum of Hypernuclei

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Chinese Physics Letters 2011年第9期28卷 80-83页

作者： song chun-yan YAO Jiang-Ming MENG Jie State Key Laboratory of Software Development Environment and School of Physics and Nuclear Energy EngineeringBeihang UniversityBeijing 100191 School of Physical Science and Technology Southwest UniversityChongqing 400715 State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871 School of Physics and Nuclear Energy Engineering Beihang UniversityBeijing 100191 Department of Physics University of StellenboschStellenboschSouth Africa

Effects ofΛΛω−tensor coupling on the spin symmetry ofΛspectra inΛ−nucleus systems are studied using relativistic mean-field *** 12C+Λas an example,it is found that the tensor coupling enlarges the spin−orbit splittings ofΛby a factor of 5 but has a negligible effect on the wave functions ofΛ.Similar conclusions are observed in otherΛ−nuclei,including ^(16)O+Λ,^(40)Ca+Λand ^(208)Pb+Λ.It is indicated that the spin symmetry in anti-lambda-nucleus systems is still a good approximation irrespective of the tensor coupling.

关键词： theory tensor symmetry

Changes in grain-filling characteristics of single-cross maize hybrids released in China from 1964 to 2014

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Journal of Integrative Agriculture 2023年第3期22卷 691-700页

作者： GAO Xing LI Yong-xiang yanG Ming-tao LI chun-hui song yan-chun WANG Tian-yu LI Yu SHI Yun-su Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Grain filling is the physiological process for determining the obtainment of yield in cereal *** grain-filling characteristics of 50 maize brand hybrids released from 1964 to 2014 in China were assayed across multiple *** found that the grain-filling duration(54.46%)and rate(43.40%)at the effective grain-filling phase greatly contributed to the final performance parameter of 100-kernel weight(HKW).Meanwhile,along with the significant increase in HKW,the accumulated growing degree days(GDDs)for the actual grain-filling period duration(AFPD)among the selected brand hybrids released from the 1960s to the 2010s in China had a decadal increase of 23.41℃ ***,there was a decadal increase of only 19.76℃ d for GDDs of the days from sowing to physiological maturity(DPM),which was also demonstrated by a continuous decrease in the ratio between the days from sowing to silking(DS)and DPM(i.e.,from 53.24%in the 1960s to 49.78%in the 2010s).In contrast,there were no significant changes in grain-filling rate along with the release years of the selected ***,the stability of grain-filling characteristics across environments also significantly increased along with the hybrid release *** also found that the exotic hybrids showed a longer grain-filling duration at the effective grain-filling phase and more stability of the grain-filling characteristics than those of the Chinese local *** to the results of this study,it is expected that the relatively longer grain-filling duration,shorter DS,higher grain-filling rate,and steady grain-filling characteristics would contribute to the yield improvement of maize hybrids in the future.

关键词： maize(Zea mays L.) grain-filling rate grain-filling duration stability

Haplotypic Structure and Allelic Variation of rab17,an ABA-Responsive Gene,in a Mini Core Set of Chinese Diversified Maize Inbred Lines

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Agricultural Sciences in China 2010年第12期9卷 1726-1738页

作者： YU Yong-tao WANG Rong-huan SHI Yun-su song yan-chun WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences/National Facility for Gene Resources and Genetic Improvement Beijing 100081 P.R. China Crop Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 P.R.China

Drought stress is one of the most important factors limiting maize production. Rab17 is an ABA-responsive gene and associated with drought tolerance. In order to identify haplotypic structure and mine allelic variants at tab17 locus, nucleotide diversity and linkage disequilibrium （LD） structure of rab17 were evaluated among a mini core set of Chinese diversified maize inbred lines. Totally, 19 SNP and 18 insertion/deletions （InDels） were identified, among which 81% were in non-coding regions and 19% in coding regions. The results showed that a high level of diversity appeared within 1 kb upstream of the rabl 7 locus, and declined quickly downstream of the gene region. Rapid decay of linkage disequilibrium of rabl 7 region with distance within 1 kb was detected. Functional markers which can be developed based on haplotype 14 are expected to have contribution to molecular breeding for drought tolerance.

关键词： allelic variation drought tolerance linkage disequilibrium maize rab17

Transcriptomic profiling of sorghum leaves and roots responsive to drought stress at the seedling stage

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Journal of Integrative Agriculture 2019年第9期18卷 1980-1995页

作者： ZHANG Deng-feng ZENG Ting-ru LIU Xu-yang GAO Chen-xi LI Yong-xiang LI chun-hui song yan-chun SHI Yun-su WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Drought stress affects the growth and productivity of crop plants including *** study the molecular basis of drought tolerance in sorghum,we conducted the transcriptomic profiling of sorghum leaves and roots under drought stress using RNA-Seq method.A total of 510,559,and 3 687 differentially expressed genes(DEGs)in leaves,3 368,5 093,and 4 635 DEGs in roots responding to mild drought,severe drought,and re-watering treatments were identified,*** them,190 common DEGs in leaves and 1 644 common DEGs in roots were responsive to mild drought,severe drought,and re-watering *** Ontology(GO)enrichment analysis revealed that the GO categories related to drought tolerance include terms related to response to stimulus especially response to water deprivation,abscisic acid stimulus,and reactive oxygen *** major transcription factor genes responsive to drought stress include heat stress transcription factor(HSF),ethylene-responsive transcription factor(ERF),Petunia NAM,Arabidopsis ATAF1/2 and CUC2(NAC),WRKY transcription factor(WRKY),homeodomain leucine zipper transcription factor(HD-ZIP),basic helix-loop-helix transcription factor(bHLH),and V-myb myeloblastosis viral oncogene homolog transcription facotr(MYB).Functional protein genes for heat shock protein(HSPs),late-embryogenesis-abundant protein(LEAs),chaperones,aquaporins,and expansins might play important roles in sorghum drought ***,the genomic regions enriched with HSP,expansin,and aquaporin genes responsive to drought stress could be used as powerful targets for improvement of drought tolerance in sorghum and other ***,our results provide a genome-wide analysis of DEGs in sorghum leaves and roots under mild drought,severe drought,and re-watering *** study contributes to a better understanding of the molecular basis of drought tolerance of sorghum and can be useful for crop improvement.

关键词： sorghum(Sorghum bicolor L.) transcriptomic profiling drought leaves roots

Isolation and Analysis of Drought-Induced Genes in Maize Roots

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Agricultural Sciences in China 2009年第2期8卷 129-136页

作者： LI Hui-yong HUANG Shu-hua SHI Yun-su song yan-chun ZHONG Zhong-bao WANG Guo-ying WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China The Cereal Crops Institute Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China

Maize roots are important component for plant adaptation to soil water deficits because they are supposed to take up water and necessary solutes from the soil. In the present study, the drought-induced genes were isolated in maize roots. A suppression subtractive hybridization protocol was applied to construct a forward subtractive cDNA library from CN165 for drought-stressed maize roots and a number of drought-induced genes were isolated. Totally, 126 uniESTs （containing 82 singlets and 44 contigs） were obtained from 503 available ESTs sequences after macroarray hybridization. UniESTs were analyzed using BLASTN and BLASTX and the results showed that 92% of the uniESTs had homolgous sequences in maize nr database by BLASTN. About 89% of uniESTs appeared the homlogous amino acid sequences in rice protein database but not in maize protein database by BLASTX, implying that those genes are likely new functional genes in maize. Function analysis showed that those genes were involved in a broad spectrum of biological pathways, mainly in signaling and regulatory pathways related to stress tolerance.

关键词： maize roots drought stress suppression subtractive hybridization expressed sequence tag

Stability of QTL Across Environments and QTL-by-Environment Interactions for Plant and Ear Height in Maize

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Agricultural Sciences in China 2010年第10期9卷 1400-1412页

作者： ZHANG yan LI Yong-xiang WANG Wang LIU Zhi-zhai LIU Cheng PENG Bo TAN Wei-wei WANG Di SHI Yun-su SUN Bao-cheng song yan-chun WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Food Crops Xinjiang Academy of Agricultural Sciences Urumqi 830000 P.R.China Maize Research Institute Southwest University Chongqing 400716 P.R.China

Better understanding of genotype-by-environment interaction （GEI） is expected to provide a solid foundation for genetic improvement of crop productivity especially under drought-prone environments. To elucidate the genetic basis of the plant and ear height, 2 F2：3 populations were derived from the crosses of Qi 319 × Huangzaosi （Q/H） and Ye 478 × Huangzaosi （Y/H） with 230 and 235 families, respectively, and their parents were evaluated under 3 diverse environments in Henan, Beijing, and Xinjiang, China during the year of 2007 and 2008, and all the lines were also evaluated under water stress environment. The mapping results showed that a total of 21 and 12 QTLs were identified for plant height in the Q/H and Y/H population, respectively, and 24 and 13 QTLs for ear height, respectively. About 56 and 73% of the QTLs for 2 traits did not present significant QTL-by-environment interaction （QE1） in the normal joint analyses for Q/H and Y/H population, respectively, and about 73% of the QTLs detected did not show significant QEI according to joint analyses for stress condition in Q/H. Most of the detected major QTLs exhibited high stability across different environments. Besides, several major QTLs were detected with large and consistent effect under normal condition （Chr. 6 and 7 in Q/H; Chr. 1, 3 and 9 in Y/H）, or across 2 water regimes （Chr. 1, 8 and 10 for in Q/H）. There were several constitutive QTLs （3 for Q/H and 1 for Y/H） with no or minor QTL-by-environment for the 2 populations. Finally, we found several genomic regions （Chr. 1, 10, etc.） to be co-located across the populations, which could provide useful reference for genetic improvement of these traits in maize breeding programs. Comparative genomic analysis revealed that 3 genes/genetic segments associated with plant height in rice were orthologous to these 3 identified genomic regions carrying the major QTLs for plant and ear height on Chr. 1, 6, and 8, respectively.

关键词： maize (Zea mays L.) stability QTL QTL-by-environment plant height ear height

Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing

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Journal of Integrative Agriculture 2020年第2期19卷 449-464页

作者： HAO Lu-yang LIU Xu-yang ZHANG Xiao-jing SUN Bao-cheng LIU Cheng ZHANG Deng-feng TANG Huai-jun LI chun-hui LI Yong-xiang SHI Yun-su XIE Xiao-qing song yan-chun WANG Tian-yu LI Yu Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Grain Crops Xinjiang Academy of Agricultural SciencesUrumqi 830002P.R.China

Drought is one of the most important abiotic stresses affecting maize growth and development and therefore resulting in yield *** it is essential to understand molecular mechanisms of drought stress responses in maize for drought tolerance *** root plays a critical role in plants sensing water *** the present study,two maize inbred lines,H082183,a drought-tolerant line,and Lv28,a drought-sensitive line,were grown in the field and treated with different water conditions(moderate drought,severe drought,and well-watered conditions)during vegetative *** transcriptomes of their roots were investigated by RNA *** were 1428 and 512 drought-responsive genes(DRGs)in Lv28,688 and 3363 DRGs in H082183 under moderate drought and severe drought,respectively.A total of 31 Gene Ontology(GO)terms were significantly over-represented in the two lines,13 of which were enriched only in the DRGs of *** on results of Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis,"plant hormone signal transduction"and"starch and sucrose metabolism"were enriched in both of the two lines,while"phenylpropanoid biosynthesis"was only enriched in *** analysis revealed the different expression patterns of genes related to abscisic acid(ABA)signal pathway,trehalose biosynthesis,reactive oxygen scavenging,and transcription factors might contribute to drought tolerance in *** results contribute to illustrating drought-responsive molecular mechanisms and providing gene resources for maize drought improvement.

关键词： maize(Zea mays L.) root transcriptome RNA sequencing drought-responsive genes

Simple nonlinear model for the relationship between maize yield and cumulative water amount

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Journal of Integrative Agriculture 2017年第4期16卷 858-866页

作者： LIU Cheng SUN Bao-cheng TANG Huai-jun WANG Tian-yu LI Yu ZHANG Deng-feng XIE Xiao-qing SHI Yun-su song yan-chun yanG Xiao-hong LI Jian-sheng Maize Research Center China Agricultural University Institute of Grain Crops Xinjiang Academy of Agricultural Sciences Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Both the additive and multiplicative models of crop yield and water supply are polynomial equations, and the number of parameters increases linearly when the growing period is specified. However, interactions among multiple parameters occasionally lead to unreasonable estimations of certain parameters, which were water sensitivity coefficients but with negative value. Additionally, evapotranspiration must be measured as a model input. To facilitate the application of these models and overcome the aforementioned shortcomings, a simple model with only three parameters was derived in this paper based on certain general quantitative relations of crop yield （Y） and water supply （W）. The new model, Y/Y-W＊/（W＊＋w＊）, fits an S or a saturated curve of crop yield with the cumulative amount of water. Three parameters are related to biological factors： the yield potential （Y＊）, the water requirement to achieve half of the yield potential （half-yield water requirement, wh）, and the water sensitivity coefficient （k）. The model was validated with data from 24 maize lines obtained in the present study and 17 maize hybrids published by other authors. The results showed that the model was well fit to the data, and the normal root of the mean square error （NRMSE） values were 2.8 to 17.8% （average 7.2%） for the 24 maize lines and 2.7 to 12.7% （average 7.4%） for the 17 maize varieties. According to the present model, the maize water-sensitive stages in descending order were pollen shedding and silking, tasselling, jointing, initial grain filling, germination, middle grain filling, late grain filling, and end of grain filling. This sequence was consistent with actual observations in the maize field. The present model may be easily used to analyse the water use efficiency and drought tolerance of maize at specific stages.

关键词： yield water model maize water sensitivity drought tolerance

Isolating Soil Drought-Induced Genes from Maize Seedling Leaves Through Suppression Subtractive Hybridization

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Agricultural Sciences in China 2007年第6期6卷 647-651页

作者： LI Hui-yong HUANG Su-hua SHI Yun-su song yan-chun ZHAO Jiu-ran WANG Feng-ge WANG Tian-yu LI Yu Institute of Food Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Maize Research Institute Beijing Academy of Agricultural and Forestry Sciences Beijing 100081 P.R.China

In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.

关键词： maize drought stress suppression subtractive hybridization (SSH) uniESTs