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Time-lapse changes of in vivo injured neuronal substructures in the central nervous system after low energy two-photon nanosurgery

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Neural Regeneration Research 2017年第5期12卷 751-756页

作者： Zhikai Zhao Shuangxi Chen Yunhao Luo Jing Li smaranda badea Chaoran Ren Wutian Wu Guangdong-Hong Kong-Macao Institute of CNS Regeneration Jinan University Guangzhou Guangdong Province China School of Biomedical Sciences Division of Anatomy The University of Hong Kong Pokfulam Hong Kong Special Administrative Region China Guangdong Engineering Research Center of Stem Cell Storage and Clinical Application Saliai Stem Cell Science and Technology Guangzhou Guangdong Province China State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong Pokfulam Hong Kong Special Administrative Region China

There is currently very little research regarding the dynamics of the subcellular degenerative events that occur in the central nervous system in response to injury. To date, multi-photon excitation has been primarily used for imaging applications; however, it has been recently used to selectively disrupt neural structures in living animals. However, understanding the complicated processes and the essential underlying molecular pathways involved in these dynamic events is necessary for studying the underlying process that promotes neuronal regeneration. In this study, we introduced a novel method allowing in vivo use of low energy（less than 30 m W） two-photon nanosurgery to selectively disrupt individual dendrites, axons, and dendritic spines in the murine brain and spinal cord to accurately monitor the time-lapse changes in the injured neuronal structures. Individual axons, dendrites, and dendritic spines in the brain and spinal cord were successfully ablated and in vivo imaging revealed the time-lapse alterations in these structures in response to the two-photon nanosurgery induced lesion. The energy（less than 30 m W） used in this study was very low and caused no observable additional damage in the neuronal sub-structures that occur frequently, especially in dendritic spines, with current commonly used methods using high energy levels. In addition, our approach includes the option of monitoring the time-varying dynamics to control the degree of lesion. The method presented here may be used to provide new insight into the growth of axons and dendrites in response to acute injury.

关键词： nerve regeneration dendrite dendritic spine axon spinal cord two-photon nanosurgery single-synapse resolution neural regeneration

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Targeting PTP σ ameliorates morphological and behavioral outcomes after experimental ICH in mice

Targeting PTP σ ameliorates morphological and behavioral ou...

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第十四届中国医师协会神经外科医师年会

作者： Haitao Sun Min Yao Hei Ming Lai Yin Stephen Chen smaranda Ruxandra badea Jiandong Huang Gilberto Ka Kit Leung Wutian Wu Department of Neurosurgery Zhujiang HospitalSouthern Medical University School of Biomedical Sciences LKS Faculty of Medicinethe University of Hong Kong Department of Surgery LKS Faculty of Medicinethe University of Hong Kong State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong GHM Institute of CNS regeneration Jinan University

＜正＞Objective: To investigate effects of treatment with ISP targeting PTPσ on behavioral and morphological outcomes after experimental ICH in mice. Methods: Experimental ICH model in male mice was induced by intrastriatal injection of Collagenase IV. The functional neurological recovery were evaluated weekly until 8 weeks post-ICH using rotarod test and Cylinder test following daily ISP（10μM）

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