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Hybrid assembly of conducting nanofiber network for ultra-stretchable and highly sensitive conductive hydrogels

Journal of Materials Science & Technology 2024年第2期169卷 1-10页

作者： Yalei Wang shulong zeng Shaohong Shi Yuheng Jiang Zhiwei Du Bingzhen Wang Xiurong Li State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures School of ResourcesEnvironment and MaterialsGuangxi UniversityNanning 530004China State Key Laboratory of Polymer Materials Engineering Polymer Research Institute of Sichuan UniversitySichuan UniversityChengdu 610065China

Conductive hydrogels have attracted extensive attention owing to their promising application prospects in flexible and wearable ***,achieving both high sensitivity and mechanical robustness remains ***,a novel and versatile conductive hydrogel based on the hybrid assem-bly of conductive cellulose nanofiber(CNF)networks has been designed and *** by the templating effect of CNFs and stabilizing effect of negatively charged poly(styrene sulfonate)(PSS),conducting polymer poly(3,4-ethylenedioxythiophene)(PEDOT)was self-organized into three-dimensional nanostructures which constructed a robust conductive network after in-situ oxidative *** unique structure derived from CNF bio-template endowed polyacrylamide(PAM)hydrogels with improved electrical conductivity and excellent mechanical *** a result,the as-fabricated CNF/PEDOT:PSS/PAM hydrogel exhibited an ultimate tensile strain of 1881%and toughness of 3.72 MJ/m^(3),which were 4.07 and 8.27 times higher than the CNF-free hydrogel,*** significantly,the resultant hydrogel sensor showed highly desirable sensing properties,including remarkable sensing range(1100%),high gauge factor(GF=5.16),fast response time(185 ms),and commendable durability,as well as good ***,the hydrogel sensor was able to distinguish subtle physiological activities including phonation and facial expression,and monitor large human body motions such as finger flexion and elbow ***,it was feasible to integrate the strain sensor on the joints of robots to recognize complicated machine motion signals,showing potential in advanced human-machine interactions.

关键词： Strain sensor Cellulose nanofibers PEDOT:PSS Conductive hydrogel Templating method

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α成核剂与增刚剂对不同相对分子质量聚丙烯的影响

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塑料 2022年第2期51卷 23-28,33页

作者：曾书龙李怡俊吴磊界首市天鸿新材料股份有限公司安徽界首236500 高分子材料工程国家重点实验室(四川大学) 四川大学高分子研究所四川成都610065

采用熔融共混的方法制备了可用于扭结包装的聚丙烯(PP)/二苄叉山梨醇类α成核剂DBS/聚烯烃增刚剂三元复合薄膜,对比并分析了PP的熔体流动速率(MFR)、增刚剂与DBS添加含量对薄膜结构与性能的影响。结果表明,添加增刚剂和DBS后,PP基体出... 详细信息

采用熔融共混的方法制备了可用于扭结包装的聚丙烯(PP)/二苄叉山梨醇类α成核剂DBS/聚烯烃增刚剂三元复合薄膜,对比并分析了PP的熔体流动速率(MFR)、增刚剂与DBS添加含量对薄膜结构与性能的影响。结果表明,添加增刚剂和DBS后,PP基体出现相分离的结构;采用偏光显微镜(POM)与DSC分析了其对PP结晶性能的影响,结果表明,增刚剂对PP结晶性能的影响较小,但是,当添加DBS后,PP的结晶度和结晶温度显著提高,MFR为12 g/10min的PP在添加1%DBS后,结晶度从47.7%提高至52.6%;而且,添加增刚剂与DBS后,PP的杨氏模量显著提高,断裂伸长率显著降低,当增刚剂、DBS的含量分别为3%、1%时,MFR为12 g/10min(3MDPP-12)时的PP模量达到1459 MPa,与纯样相比,模量提升了120%,但是,断裂伸长率从887%下降至7.7%。

关键词：聚丙烯 α成核剂增刚剂扭结膜结晶

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The“China Story”and China's International Image

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Contemporary International Relations 2015年第6期25卷 14-27页

作者： Chu shulong zeng Qiang the Institute of International Strategy and Development of Tsinghua University

We Chinese should not tell false stories, or deny or cover up ＂stories＂ that are not so good, and at the same time we should not allow wrongversions and interpretations of our story to become accepted as the standard. With the international audience watching China＇s amazing rise, the country translators and foreign language experts should become more skilled in matching Chinese connotations to those of foreign languages to allow the ＂China story＂ to be promulgated sincerely in form and content. China＇s leaders, beginning with President Xi Jinping, have emphasized the importance of this. At the request of China＇s leaders, the challenge is to correctly publicize ＂Chinese stories,＂ improve China＇s international image, promote mutual understanding, and boost the peaceful development of China and the world. The Foreign Affairs Work Conference of the CPC Central Committee that convened in November of 2014 expressed the need ＂to advance China＇s soft power, well publicize ＇Chinese stories,＇ and do well the work of foreign publicizing.

关键词：国际形象中国人故事世界和平宣传工作领导人相匹配习近平

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基于“双壁理论”探讨ARCOⅡ期股骨头坏死的保髋治疗策略

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中医正骨 2024年第9期36卷 76-82页

作者：牛驰程曾平魏秋实韦标方刘金富周树龙王伟伟许青源李豪广西中医药大学研究生院广西南宁530001 广西中医药大学第一附属医院广西南宁530023 广州中医药大学第三附属医院广东广州510378 临沂市人民医院山东临沂276003

保髋治疗对于青壮年股骨头坏死(osteonecrosis of the femoral head,ONFH)患者至关重要,尤其是国际骨微循环研究学会(Association Research Circulation Osseous,ARCO)Ⅱ期,被认为是进行保髋治疗的黄金时期。如何选择合适的保髋治疗方... 详细信息

保髋治疗对于青壮年股骨头坏死(osteonecrosis of the femoral head,ONFH)患者至关重要,尤其是国际骨微循环研究学会(Association Research Circulation Osseous,ARCO)Ⅱ期,被认为是进行保髋治疗的黄金时期。如何选择合适的保髋治疗方式并提高疗效,是临床亟待解决的问题。“双壁理论”根据股骨头的坏死范围是否累及前侧壁和外侧壁将ONFH分为3种类型,即双壁完好型、单壁受累型、双壁受累型,不同分型的治疗方法也不同。本文概述了“双壁理论”,并基于该理论探讨了ARCOⅡ期ONFH的保髋治疗策略。

关键词：股骨头坏死中医药治法外科手术 ARCOⅡ期双壁理论

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Ultraviolet-infrared dual-color photodetector based on vertical GaN nanowire array and graphene

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Chinese Optics Letters 2020年第11期18卷 72-76页

作者： Chunhong zeng Wenkui Lin Tao He Yukun Zhao Yuhua Sun Qi Cui Xuan Zhang shulong Lu Xuemin Zhang Yameng Xu Mei Kong Baoshun Zhang School of Science Changchun University of Science and TechnologyChangchun 130022China Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of SciencesSuzhou 215123China School of Materials Science and Engineering Nanjing University of Science and TechnologyNanjing 210094China

A monolithic integrated ultraviolet-infrared(UV-IR) dual-color photodetector based on graphene/GaN heterojunction was fabricated by vertically integrating a GaN nanowire array on a silicon substrate with monolayer graphene. The device detects UV and IR lights by different mechanisms. The UV detection is accomplished by the forbidden band absorption of GaN, and the IR detection is realized by the free electron absorption of graphene. At peak wavelengths of 360 nm and 1540 nm, the detector has responsivities up to 6.93 A/W and 0.11 A/W, detection efficiencies of 1.23 × 1012 cm·Hz1/2·W-1 and 1.88 × 1010 cm·Hz1/2·W-1, respectively,and a short response time of less than 3 ms.

关键词： UV-IR dual-color photodetector heterojunction GaN nanowire array graphene

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连续性肾脏替代治疗救治恶性综合征二例

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中华精神科杂志 2018年第3期51卷 218-220页

作者：李树龙曾智东莞康华医院精神科 523080

例1患者女性，49岁，因“言语行为紊乱20余年，发热伴意识改变1d”于2016年12月13日收入我院精神科。患者确诊精神分裂症20余年，予利培酮治疗，最大剂量6mg／d，入院前3年内不规律服用利培酮2mg/d。入院前1d患者出现发热（具体体温不... 详细信息

例1患者女性，49岁，因“言语行为紊乱20余年，发热伴意识改变1d”于2016年12月13日收入我院精神科。患者确诊精神分裂症20余年，予利培酮治疗，最大剂量6mg／d，入院前3年内不规律服用利培酮2mg/d。入院前1d患者出现发热（具体体温不详），意识不清，行动缓慢，乏力，多汗，大部分时间不动不语，饮食减少，睡眠差。入院体检：体温40℃，脉搏152次／min，呼吸32次／min，血压131／65mmHg（1mmHg=0．133kPa，血压持续下降，最低至74／43mmHg）。

关键词：连续性肾脏替代治疗恶性综合征救治精神分裂症行为紊乱意识改变最大剂量意识不清

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Design and evaluation of a novel HSV-2 epitope vaccine

Design and evaluation of a novel HSV-2 epitope vaccine

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Wenwen zeng Yong Qi Jiameng Li Suyun Liu Jixain Rao Wanpeng Shen shulong Zheng Yuexi Li Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University

We used LCP technology to design and synthesize a lipid core peptide vaccine with self-assembly and intramolecular adjuvant properties for HSV-2 epitope,and evaluate the immune *** this study,LCPs were designed for two B cell epitopes gB466-473(EQDRKPRN) and gC216-223(GRTDRPSA),respectively named LCP-1 and LCP-2 a,LCP-2 *** other peptide including P1-P25,P1-P25-KLH,P2-P25,*** in nine groups were intramuscularly immunized with 30 μg of each antigen per mouse in the hind limbs with LCP1,P1-P25,P1-P25-KLH,and MF59 adjuvanted P1-P25,LCP2 a,LCP2 b,P2-P25,P2-P25-KLH,and MF59 adjuvanted P2-P25 respectively,In peptide 1 experiment,mice were immunized for four times on days 0,21 st,35 th,and 49 th *** peptide 2 experiment,mice were immunized for three ***-P1-BSA or anti-P2-BSA IgG,IgG1,and IgG2 a antibody titers were evaluated using ELISA and analyzed statistically in sera after each ***1 and P1-P25-KLH induced the highest IgG and IgG1 antibody *** and IgG1 titers induced by LCP2 a were higher than other groups after the second or third ***,none peptide groups induced IgG2 a *** the T cell responses,CD4 and CD8 T cells isolated from immunized mice were stimulated with corresponding peptides and cytokines in the supernatant were *** a result,none of the cytokines were *** HSV-2 virus neutralization activity of mouse serum after the last immunization was measured by a 50% plaque reduction ***1-induced antisera performed a higher neutralization titer than the other three *** summary,this study designed,synthesized,and evaluated two lipid epitopes of B-cell epitopes that all showed good humoral ***1 and LCP2 b could stimulate mice into producing specific antibodies that could neutralize HSV-2 infection in *** results suggest that the lipid core peptide vaccine may be used for vaccine design against HSV-2.

关键词： Herpes simplex virus type 2(HSV-2) humoral immunity cellular immunity virus-neutralizing activity

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Effect of human umbilical cord mesenchymal stem cells on LPS-induced macrophage inflammation

Effect of human umbilical cord mesenchymal stem cells on LPS...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Jiameng Li shulong Zheng Wenwen zeng Suyun Liu Jixain Rao Wanpeng Shen Yong Qi Yuexi Li China Pharmaceutical University

OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells(huMSCs) on the expression of IL-1β,IL-6,TNF-α and IFN-β in lipopolysaccharide(LPS)-induced macrophage inflammation by cell ***: Human umbilical cord mesenchymal stem cells(huMSCs) and mouse macrophages(RAW264.7) were cocultured with transwell to extract total RNA from macrophages at 4,8,and 12 *** real-time quantitative PCR was used to detect the difference of IL-1β,IL-6,TNF-α and IFN-β mRNA expression in different experimental *** exosomes in the culture supernatant of mesenchymal stem cells were extracted by the kit,co-cultured with LPS-induced macrophages,macrophage supernatant was collected at 4,8,and 12 hours,The expressions of IL-1β,IL-6,TNF-α and IFN-β in the supernatant of different experimental groups were detected by ***: The results of real-time quantitative PCR were consistent with those of *** expressions of IL-1β,IL-6,TNF-α and IFN-β mRNA and protein were the highest in the model group,and the expression in the co-culture group was *** expression level of the negative reference group was the lowest;the total expression of each group increased at different time points,but the expression of mRNA and protein in the co-culture group was still lower than that of the model ***: Human umbilical cord mesenchymal stem cells can inhibit the expression of IL-1β,IL-6,TNF-α and IFN-β mRNA and protein in LPS-induced inflammatory injury.

关键词： Mesenchymal stem cells Lipopolysaccharide Inflammation

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Expression and purification of proteins related to Recombinase polymerase amplification

Expression and purification of proteins related to Recombina...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Suyun Liu Yong Qi Jiameng Li Wenwen zeng Jixain Rao Wanpeng Shen shulong Zheng Yuexi Li Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University

Recombinase polymerase amplification(RPA) is a novel nucleic acid amplification technology that does not require repeated control of temperature and precision *** has the characteristics of high sensitivity,strong specificity and fast *** RPA system consists of three interacting enzymes,a recombinase(UvsX),a single-stranded binding protein(Gp32),and a polymerase(Bsu),and an accessory protein(UvsY).In this study,three recombinant enzymes,two accessory proteins and a single-stranded binding protein sequence were screened,and the His tag was fused to the C-terminus of UvsX,the N-terminus of UvsY and the C-terminus of Gp32,respectively,and cloned into PET-28(+) *** constructed recombinant plasmid was transformed into *** BL21(DE3),induced by IPTG,and the strain expressing the fusion protein was screened by *** cultured high-expression strain of the fusion protein was expanded,and the expressed recombinant protein was purified to detect the purity and concentration of the *** results showed that the expression of UvsX,UvsY and Gp32 were soluble when induced at 25 °C,and could be purified by nickel column to obtain protein with high purity and *** has good application value in the field of recombinase polymerase amplification technology.

关键词： recombinase soluble expression purification application

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Design and Evaluation of A Novel HSV-2 epitope Vaccine

Design and Evaluation of A Novel HSV-2 epitope Vaccine

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中国生物工程学会第十二届学术年会暨2018年全国生物技术大会

作者： Wenwen zeng Yong Qi Jiameng Li Suyun Liu shulong Zheng Jixian Rao Wanpeng Shen Yuexi Li Department of Medical and Pharmaceutical Biotechnology Huadong Research Institute for Medicine and Biotechniques School of Life Science and Technology China Pharmaceutical University

We used LCP technology to design and synthesize a lipid core peptide vaccine with self-assembly and intramolecular adjuvant properties for the pre-expressed HSV-2 epitope, and evaluate the immune effect. In this study, LCPs were designed for two B cell epitopes gB466-473(EQDRKPRN) and gC216-223(GRTDRPSA), respectively named P1-(K)2-LC and P2-(K)2-LC, *** other peptide including P1-P25, P1-P25-KLH, P2-P25, P2-P25-KLH. Mice in nine groups were intramuscularly immunized with 30 μg of each antigen per mousein the hind limbs with P1-(K)2-LC, P1-P25, P1-P25-KLH, and MF59 adjuvanted P1-P25, P2-(K)2-LC, P2-LC, P2-P25, P2-P25-KLH, and MF59 adjuvanted P2-P25 respectively, In peptide 1 and peptide 2 experiment, mice were immunized for three times. Anti-P1-BSA or anti-P2-BSA IgG, IgG1, and IgG2 a antibody titers were evaluated using ELISA and analyzed statistically in sera after each immunization. P1-(K)2-LC and P1-P25-KLH induced the highest IgG and IgG1 antibody titer. IgG and IgG1 titers induced by P2-(K)2-LC were higher than other groups after the second or third immunization. However, none peptide groups induced IgG2 a *** the T cell responses, CD4 and CD8 T cells isolated from immunized mice were stimulated with corresponding peptides and cytokines in the supernatant were detected. As a result, none of the cytokines were *** HSV-2 virus neutralization activity of mouse serum after the last immunization was measured by a 50% plaque reduction assay. P1-(K)2-LC induced antisera performed a higher neutralization titer than the other three *** summary, this study designed, synthesized, and evaluated two lipid epitopes of B-cell epitopes that all showed good humoral immunity. P1-(K)2-LC and P2-LC could stimulate mice into producing specific antibodies that could neutralize HSV-2 infection in *** results suggest that the lipid core peptide vaccine may be used for vaccine design against HSV-2.

关键词： HSV-2 (Herpes simplex virus type 2) humoral immunity cellular immunity virus-neutralizing activity

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