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Peptide-substituted oligonucleotide synthesis and non-toxic,passive cell delivery

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Signal Transduction and Targeted Therapy 2016年第1期1卷 52-60页

作者： shiying shang Luca Monfregola Marvin H Caruthers Department of Chemistry and Biochemistry University of ColoradoBoulderColoradoUSA

Chemically modified oligodeoxynucleotides(ODNs)are known to modulate gene expression by interacting with *** efficient approach for synthesizing amino acid-or peptide-substituted triazolylphosphonate analogs(TP ODNs)has been developed to provide improved stability and cell *** chemistry is quite general,as peptides can be introduced throughout the TP ODN at any preselected internucleotide *** synthetic TP ODNs enter cells through endocytosis in the absence of transfection reagents and localize into perinuclear *** entrapped ODNs are released into the cytoplasm by treatment with endosomal-releasing agents and several are then active as microRNA inhibitors.

关键词： substituted synthesis analogs

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固体发动机药柱热线粘弹性边界元分析

推进技术 1993年第2期14卷 57-62,73页

作者：尚世英李庆中西北工业大学

采用遗传积分形式的本构关系,推导了热线粘弹性材料时域边界元公式。由于把与时间有关的剪切模量G(t)表示成Pnony级数的形式,因而可用递推公式较方便地计算粘弹性记忆应力。本文提出的方法比以前的对应原理方法有较广的应用范围。为了... 详细信息

采用遗传积分形式的本构关系,推导了热线粘弹性材料时域边界元公式。由于把与时间有关的剪切模量G(t)表示成Pnony级数的形式,因而可用递推公式较方便地计算粘弹性记忆应力。本文提出的方法比以前的对应原理方法有较广的应用范围。为了验证提出的方法,编制了二维粘弹性边界元程序。两个算例的数值结果表明:本方法具有精度高,计算机解题快,占内存少等优点。

关键词：边界元固体推进剂粘弹性药柱

Binding of the SARS-CoV-2 spike protein to glycans

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Science Bulletin 2021年第12期66卷 1205-1214,M0004页

作者： Wei Hao Bo Ma Ziheng Li Xiaoyu Wang Xiaopan Gao Yaohao Li Bo Qin shiying shang Sheng Cui Zhongping Tan NHC Key Laboratory of Systems Biology of Pathogens Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Chemistry and Biochemistry and BioFrontiers Institute University of ColoradoBoulder CO 80303USA School of Pharmaceutical Sciences Tsinghua UniversityBeijing W0084China

The pandemic of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a high number of deaths in the *** combat it,it is necessary to develop a better understanding of how the virus infects host *** normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate(HS)and sialic acid-containing glycolipids/*** this study,we examined and compared the binding of the subunits and spike(S)proteins of SARS-CoV-2,SARS-Co V,and Middle East respiratory disease(MERS)-Co V to these *** results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was ***,this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells,and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses.

关键词： SARS-CoV-2 S protein Heparan sulfate Glycan microarray Surface plasmon resonance Sulfation Sialic acid

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以特色服务促进药学科研条件平台可持续发展

实验技术与管理 2021年第4期38卷 7-10页

作者：商世瑛唐煜尹航清华大学药学技术中心北京100084

科研条件平台在研究型大学发挥着重要的作用。除了专业技术人员、实验空间、仪器设备等关键资源外,特色发展的规划对科研条件平台的稳定和可持续性发展也起着至关重要的作用。结合药学学科的特色服务,药学技术中心在仪器设备层面实现了... 详细信息

科研条件平台在研究型大学发挥着重要的作用。除了专业技术人员、实验空间、仪器设备等关键资源外,特色发展的规划对科研条件平台的稳定和可持续性发展也起着至关重要的作用。结合药学学科的特色服务,药学技术中心在仪器设备层面实现了完整性和系统化,在专业技术上力求达到高水平,在探索转化方面注重跨学科、跨领域的深度合作,通过多举措建立了有效的运行机制,保障了科研条件平台的良性运转和可持续发展。

关键词：科研条件平台可持续发展特色服务建设

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脉冲多普勒雷达重频参数的一种序贯估计方法

现代雷达 2024年第2期46卷 103-111页

作者：尚文秀袁硕拓世英刘章孟国防科技大学电子科学学院湖南长沙410073

针对逐脉冲序贯重频参数估计的场景,引入了评估变量控制对脉冲的缓存,对受漏脉冲和干扰脉冲等数据噪声污染的脉冲列进行了较高精度的参数估计;进一步对缓存区间内的脉冲采用试探整除的方法,对脉冲多普勒雷达截获脉冲列中重频切换的跳变... 详细信息

针对逐脉冲序贯重频参数估计的场景,引入了评估变量控制对脉冲的缓存,对受漏脉冲和干扰脉冲等数据噪声污染的脉冲列进行了较高精度的参数估计;进一步对缓存区间内的脉冲采用试探整除的方法,对脉冲多普勒雷达截获脉冲列中重频切换的跳变时刻进行了跟踪检测。仿真实验验证了文中所提的重频参数序贯估计方法相较于传统重频参数估计方法在序贯估计场景下的有效性和优越性。

关键词：脉冲多普勒雷达重频参数估计序贯估计

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建筑工程造价预结算审核管理的有效举措

城市建筑 2013年第12期10卷 178-178页

作者：尚世英甘肃第六建设集团股份有限公司兰州730000

随着经济水平的快速发展,如何在保证建筑工程质量的基础上,对建筑工程的造价进行管理与控制,是当前建筑行业发展过程中所要解决的问题。本文就当前建筑工程造价预结算审核中所存在的问题,提出了有效措施。

关键词：建筑工程造价预结算审核管理有效措施

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一种太阳翼对接用调姿机构虚腿调平策略

航天控制 2024年第4期42卷 78-84页

作者：商涛鲍成文寇士营王文宗石震宇天津航天机电设备研究所天津300458 天津市宇航智能装备技术企业重点实验室天津300458 太原理工大学机械与运载工程学院太原030024

针对太阳翼对接过程中姿态调整的快速性和准确性需求及人工调整不便的问题,本文设计了一种调平策略,能够根据地面状态更新调姿机构的运动学参数,利用激光跟踪仪获取调平所需位姿,采用基于运动学逆解的关节位置闭环控制实现动平台的调平... 详细信息

针对太阳翼对接过程中姿态调整的快速性和准确性需求及人工调整不便的问题,本文设计了一种调平策略,能够根据地面状态更新调姿机构的运动学参数,利用激光跟踪仪获取调平所需位姿,采用基于运动学逆解的关节位置闭环控制实现动平台的调平,避免了传统支腿升降调平法在调平过程中存在的虚腿问题,实现了调姿机构从支腿收起状态到触地支撑状态再到动平台调至水平状态的自动调节。通过仿真验证了该调平策略的可行性,为太阳翼对接用调姿机构在工程应用中存在的虚腿调平问题提供了解决思路和方法。

关键词：并联机构虚腿调平运动学逆解激光跟踪仪

Dynamic Changes of Antibodies to SARS-CoV-2 in COVID-19 Patients at Early Stage of Outbreak

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Virologica Sinica 2020年第6期35卷 744-751页

作者： Huaqing Shu Shuzhen Wang Shunan Ruan Yaxin Wang Jiancheng Zhang Yin Yuan Hong Liu Yongran Wu Ruiting Li shangwen Pan Yaqi Ouyang shiying Yuan Peng Zhou You shang Department of Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Anesthesiology and Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Jin-Yin-Tan Hospital Wuhan 430023China CAS Key Laboratory of Special Pathogens Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China

The coronavirus disease 2019(COVID-19),caused by SARS-CoV-2,has spread around the world with high *** diagnose promptly and accurately is the vital step to effectively control its *** characteristics of SARSCoV-2-specific antibodies which are important for diagnosis of infection have not been fully *** this retrospective,single-center,observational study,we enrolled the initial 131 confirmed cases of COVID-19 at Jin-Yin-Tan Hospital who had at least one-time antibody tested during their *** dynamic changes of IgM and IgG antibodies to SARS-CoV-2 nucleocapsid protein in 226 serum samples were detected by *** sensitivities of IgM and IgG ELISA detection were *** showed that the sensitivity of the IgG ELISA detection(92.5%)was significantly higher than that of the IgM(70.8%)(P\0.001).The meantimes of seroconversion for IgM and IgG were6 days and 3 days,*** IgM and IgG antibody levels peaked at around 18 days and 23 days,and then IgM fell to below the baseline level at about day 36,whereas IgG maintained at a relatively high *** conclusion,antibodies should be detected to aid in diagnosis of COVID-19 *** could be a sensitive indicator for retrospective diagnosis and contact tracing,while IgM could be an indicator of early infection.

关键词： Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Coronavirus disease 2019(COVID-19) Coronavirus Antibody Serology

Remimazolam besylate for sedation of postoperative patients in intensive care units: a phase I, open label, dose-finding study

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Chinese Medical Journal 2022年第17期135卷 2134-2136页

作者： Yun Tang Xiaobo Yang Huaqing Shu Yuan Yu Jiqian Xu shangwen Pan Xiaojing Zou shiying Yuan You shang Department of Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China

To the Editor:Remimazolam besylate is a novel,ultrashort-acting benzodiazepine with a rapid and a predictable onset and offset profile,no accumulation and extended effect,and independence of organ elimination.

关键词： finding independence elimination