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Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

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Plant Diversity 2023年第3期45卷 326-336页

作者： Yu-Wen zhang Yu-Cen shi shi-bao zhang Key Laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Yunnan Key Laboratory for Wild Plant Resources Kunming 650201YunnanChina University of Chinese Academy of Sciences Beijing 100049China Lijiang Forest Biodiversity National Observation and Research Station Kunming Institute of BotanyChinese Academy of SciencesLijiang 674100YunnanChina

Dendrobium nobile is an important medicinal and nutraceutical *** the ingredients of *** have been identified as polysaccharides,alkaloids,amino acids,flavonoids and bibenzyls,our understanding of the metabolic pathways that regulate the synthesis of these compounds is ***,we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of ***.A total of 1005 metabolites and 31,745 genes were detected in the stems of *** majority of these metabolites and genes were involved in the metabolism of carbohydrates(fructose,mannose,glucose,xylulose and starch),while some were involved in the metabolism of secondary metabolites(alkaloids,β-tyrosine,ferulic acid,4-hydroxybenzoate and chrysin).Our predicted regulatory network indicated that five genes(AROG,PYK,DXS,ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid *** analysis identified that six genes(ALDO,PMM,BGLX,EGLC,XYLB and GLGA) were involved in carbohydrate metabolism,and two genes(ADT and CYP73A) were involved in secondary metabolite *** analyses also indicated that phosphoenol-pyruvate(PEP) was a crucial bridge that connected carbohydrate to alkaloid *** regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species.

关键词： Dendrobium nobile Transcriptome Metabolome Polysaccharides Alkaloids Secondary metabolite biosynthesis

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Plant Diversity 2020年第5期42卷 362-369页

作者： Jiao Qin Wei zhang shi-bao zhang Ji-Hua Wang Key Laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201China Yunnan Key Laboratory for Wild Plant Resources KunmingYunnanChina Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnanChina Flower Research Institute Yunnan Academy of Agricultural SciencesKunmingYunnan650205China

Mycorrhizal fungi are essential for the growth and development of both epiphytic(growing on trees)and lithophytic(growing on rocks)*** studies indicate that in lowland tropical areas,orchid mycorrhizal fungal compositions are correlated with the life form(i.e.,epiphytic,lithophytic,or terrestrial)of their host *** therefore tested if a similar correlation exists in an orchid distributed at higher *** corymbosa is an endangered ornamental orchid species that can be found as a lithophyte and epiphyte in subtropical to subalpine *** on high-throughput sequencing of the fungal internal transcribed spacer 2(ITS2)-rDNA region of mycorrhizae of ***,we detected 73 putative mycorrhizal fungal Operational Taxonomic Units(OTUs).The OTUs of two dominant lineages(Cantharellales and Sebacinales)detected from *** are phylogenetically different from those of other species within the genus Coelogyne,indicating that different orchid species prefer specific mycorrhizal *** also found that the Non-metric multidimensional scaling(NMDS)plots of orchid mycorrhizal fungi were not clustered with life form,the variations among orchid mycorrhizal fungal communities of different life forms were not significant,and most of the OTUs detected from epiphytic individuals were shared by the lithophytic plants,suggesting that orchid mycorrhizal associations of *** were not affected by life *** findings provide novel insights into mycorrhizal associations with endangered ornamental orchids.

关键词： Cantharellales Coelogyninae Epiphytic Life form Orchid mycorrhizal fungi Serendipitaceae

Leaf physiological and anatomical responses of two sympatric Paphiopedilum species to temperature

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Plant Diversity 2022年第1期44卷 101-108页

作者： Jing-Qiu Feng Ji-Hua Wang shi-bao zhang Key Laboratory of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming650201YunnanChina University of Chinese Academy of Sciences Beijing100049China Flower Research Institute of Yunnan Academy of Agricultural Sciences Kunming650205YunnanChina

Paphiopedilum dianthum and *** are two endangered orchid species,with high ornamental and conservation *** are sympatric species,but their leaf anatomical traits and flowering period have significant ***,it is unclear whether the differences in leaf structure of the two species will affect their adaptabilities to ***,we investigated the leaf photosynthetic,anatomical,and flowering traits of these two species at three sites with different temperatures(Kunming,16.7±0.2°C;Puer,17.7±0.2°C;Menglun,23.3±0.2°C)in southwest *** with those at Puer and Kunming,the values of light-saturated photosynthetic rate(Pmax).stomatai conductance(gs),leaf thickness(LT),and stomatai density(SD)in both species were lower at *** values of Pmax,gs,仃,adaxial cuticle thickness(CTad)and SD in *** were higher than those of *** at the three *** with ***,there were no flowering plants of *** at *** results indicated that both species were less resistance to high temperature,and *** had a stronger adaptability to high-temperature than *** findings can provide valuable information for the conservation and cultivation of Paphiopedilum species.

关键词： Conservation Cultivation High-temperature Leaf anatomy Paphiopedilum Photosynthesis

Differences in leaf physiological and morphological traits between Camellia japonica and Camellia reticulata

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Plant Diversity 2020年第3期42卷 181-188页

作者： Ji-Hua Wang Yan-Fei Cai shi-Feng Li shi-bao zhang Flower Research Institute of Yunnan Academy of Agricultural Sciences KunmingChina Key Laboratory for Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunmingChina National Engineering Research Center for Ornamental Horticulture KunmingChina

Plants of the genus Camellia are widely cultivated throughout the world as ornamentals because of their bright and large *** widely cultivated varieties are mainly derived from the mutant lines and hybrid progenies of Camellia japonica *** Camellia reticulata *** their geographical distributions and environmental adaptabilities are significantly different,no systematic comparison has been conducted between these two *** investigate differences in how these plants have adapted to their environments,we measured photosynthesis and 20 leaf functional traits of *** and *** grown under the same *** with ***,*** showed higher values for light saturation point,light-saturated photo synthetic rate,leaf dry mass per unit area and stomatal area,but lower values for apparent quantum efficiency,leaf size,stomatal density and leaf nitrogen content per unit *** area was positively correlated with light-saturated photosynthetic rate and light saturation point,but negatively correlated with stomatal *** differences between *** and *** were mainly reflected in their adaptations to light intensity and leaf morphological *** is better adapted to high light intensity than *** difference is related to the two species’ differing life fo ***,leaf morphological traits have played an important role in the light adaptation of *** and ***,and might be first noticed and selected during the breeding *** findings will contribute to the cultivation of camellia plants.

关键词： Camellia Light adaptation Leaf traits Ornamental plant Photosynthesis

A novel weld-pool-length monitoring method based on pixel analysis in plasma arc additive manufacturing

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Advances in Manufacturing 2024年第2期12卷 335-348页

作者： bao-Ri zhang Yong-Hua shi Bionic Robot Research Department Ji Hua LaboratoryFoshan528200GuangdongPeople’s Republic of China Guangdong Provincial Engineering Research Center for Special Welding Technology and Equipment School of Mechanical and Automotive EngineeringSouth China University of TechnologyGuangzhou510640People’s Republic of China

The real-time monitoring of the weld pool during deposition is important for automatic control in plasma arc additive *** obtain a high deposition accuracy,it is essential to maintain a stable weld pool *** this study,a novel passive visual method is proposed to measure the weld pool *** the proposed method,the image quality was improved by designing a special visual system that employed an endoscope and a *** also includes pixel brightness-based and gradient-based algorithms that can adaptively detect feature points at the boundary when the weld pool geometry *** algorithm can also be applied to materials with different solidification *** was performed to measure the real weld pool length in world coordinates,and outlier rejection was performed to increase the accuracy of the ***,tests were carried out on the intersection component,and the results showed that the proposed method performed well in tracking the changing weld pool length and was applicable to the real-time monitoring of different types of materials.

关键词： Plasma arc additive manufacturing(PAAM) Weld pool geometry Gradient analysis Real-time detection

Plasmakinetic enucleation of prostate versus 160-W laser photoselective vaporization for the treatment of benign prostatic hyperplasia

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Asian Journal of Andrology 2017年第1期19卷 15-19页

作者： Si-Jun Wang Xiao-Nan Mu Ji Chen Xun-Bo Jin shi-bao zhang Long-Yang zhang Department of Urology Jinan Central Hospital Affiliated to Shandong University Jinan 250013 P.R. China Department of Concerning Foreign Affairs Jinan Central Hospital Affiliated to Shandong University Jinan 250013 P.R. China Department of Minimally Invasive Urology Provincial Hospital Affiliated to Shandong UniversityJinan 250021 P.R. China.

To evaluate the safety and efficacy of plasmakinetic enucleation of the prostate （PKEP） for the treatment of symptomatic benign prostatic hyperplasia （BPH） compared with 160-W lithium triboride laser photoselective vaporization of the prostate （PVP）. From February 2011 to July 2012, a prospective nonrandomized study was performed. One-hundred one patients underwent PKEP, and 110 underwent PVP. No severe intraoperative complications were recorded, and none of the patients in either group required a blood transfusion. Shorter catheterization time （38.14 ± 23.64 h vs 72.54 ± 28.38 h, P 〈 0.001） and hospitalization （2.32 ± 1.25 days vs 4.07±1.23 days, P 〈 0.001） were recorded in the PVP group. At 12-month postoperatively, the PKEP group had a maintained and statistically improvement in International Prostate Symptom Score （IPSS）（4.07 ±2.07 vs 5.00 ±2.10; P〈 0.001）, quality of life （QoL）（1.08 ± 0.72 vs 1.35 ± 0.72; P= 0.007）, maximal urinary flow rate （Qmax）（24.75±5.87 ml s^-1 vs 22.03 ±5.04 ml s^-1; P 〈 0.001）, postvoid residual urine volume （PVR）（14,29 ± 6,97 ml vs 17.00±6.11 ml; P = 0,001）, and prostate-specific antigen （PSA） value （0.78 ±0.57 ng ml^-1 vs 1.27 ±1.07 ng ml^-1; P 〈 0.001）. Both PKEP and PVP relieve low urinary tract symptoms （LUTS） due to BPH with low complication rates. PKEP can completely remove prostatic adenoma while the total amount of tissue removed by PVP is less than that can be removed by PKER Based on our study of the follow-up, PKEP provides better postoperative outcomes than PVP.

关键词： electrosurgery laser therapy prostate prostatic hyperplasia transurethral resection of prostate

Simulation and optimization of scrap wagon dismantling system based on Plant Simulation

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Visual Computing for Industry,Biomedicine,and Art 2023年第1期6卷 88-96页

作者： Hai-Qing Chen Yu-De Dong Fei Hu Ming-Ming Liu shi-bao zhang School of Mechanical Engineering Hefei University of TechnologyHefei 230009China China Railway Materials Group Resources Technology Co. Ltd.Hefei 230088China

Based on the existing plant layout and process flow,a simulation analysis was conducted using the Plant Simulation platform with the utilization efficiency of each station and production capacity of the dismantling system as indicators.A problem with long-term suspension in the disassembly process was *** on the two optimization directions of increasing material transportation equipment and expanding the buffer capacity,a cost-oriented optimization model is established.A genetic algorithm and model simulation were used to solve the *** optimization scheme that satisfies the production needs and has the lowest cost is *** results show that the optimized dismantling system solves the suspended work problem at the dismantling station and a significant improvement in productivity and station utilization efficiency compared with the previous system.

关键词： Plant Simulation Production optimization Wagon dismantling Genetic algorithm

Designing metal sulfide-based cathodes and separators for suppressing polysulfide shuttling in lithium-sulfur batteries

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Nano Research 2024年第4期17卷 2574-2591页

作者： Guoyin Zhu Qingzhu Wu Xianghua zhang Yuwen bao Xuan zhang Zhuoyao shi Yizhou zhang Lianbo Ma Institute of Advanced Materials and Flexible Electronics(IAMFE) School of Chemistry and Materials ScienceNanjing University of Information Science&TechnologyNanjing 210044China School of Materials Science and Engineering Anhui University of TechnologyMaanshan 243002China School of Mechanical Engineering Jiangsu University of TechnologyChangzhou 213001China

Lithium-sulfur(Li-S)batteries,known for their high energy density,are attracting extensive research interest as a promising next-generation energy storage ***,their widespread use has been hampered by certain issues,including the dissolution and migration of polysulfides,along with sluggish redox *** sulfides present a promising solution to these obstacles regarding their high electrical conductivity,strong chemical adsorption with polysulfides,and remarkable electrocatalytic capabilities for polysulfide *** this review,the recent progress on the utilization of metal sulfide for suppressing polysulfide shuttling in Li-S batteries is systematically summarized,with a special focus on sulfur hosts and functional *** critical roles of metal sulfides in realizing high-performing Li-S batteries have been comprehensively discussed by correlating the materials’structure and electrochemical ***,the remaining issues/challenges and future perspectives are *** offering a detailed understanding of the crucial roles of metal sulfides,this review dedicates to contributing valuable knowledge for the pursuit of high-efficiency Li-S batteries based on metal sulfides.

关键词： lithium-sulfur batteries metal sulfides polysulfide shuttling sulfur hosts separator coating layers

Reasonable Designing of Free-standing MnO_(2)/Graphene Composite Membrane for Lithium-ion Storage

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Chemical Research in Chinese Universities 2024年第3期40卷 508-512页

作者： shi Junye YU Chenxi ZOU Zewei ZHENG Shumin zhang Xing WANG bao Institute of Refrigeration and Cryogenics Shanghai Jiao Tong University Shanghai 200433P.R.China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190P.R.China Key Laboratory of Biopharmaceutical Preparation and Delivery Institute of Process EngineeringChinese Academy of SciencesBeijing 100190P.R.China CAS Key Laboratory of Molecular Nanostucture and Nanotechnology Institute of ChemistryChinese Academy of SciencesBeijing 100190P.R.China

Free-standing membranes show extraordinary promise in energy storage applications,however are usually limited by low capacity and poor rate ***,we assembled a series of free-standing MnO_(2)/GO composite membranes with ZIF67 particles embedded between two-dimensional(2D)*** particle size of ZIF67 can be adjusted to achieve tunable interlayer *** lithium storage performance of the as-obtained membranes with different interlayer spaces was systematically *** MnO_(2)/GO composite membrane embedded with ZIF67 particles with an average diameter of 30 nm(denoted as MnO_(2)/nZIF67/GO)delivers a good long cycling performance,and it retains a capacity of 340.7 mA·h·g^(−1) after 400 cycles at 0.05 A/*** MnO_(2)/GO composite membrane embedded with ZIF67 particles with an average diameter of 500 nm(denoted as MnO_(2)/mZIF67/GO)exhibits good rate *** of the size of the ZIF67 particles,the performance of the membrane containing ZIF67 is significantly better than that of the membrane without ZIF67,indicating that the ZIF67 particles can enhance the lithium storage performance of the assembled *** work provides a method to fabricate a free-standing membrane for lithium storage with tunable electrochemical performance.

关键词： Lithium-ion battery Membrane Free-standing ZIF67