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脱脂油棕谷蛋白2的营养价值与功能特性

食品工业科技 2020年第8期41卷 311-315页

作者：李艳史攀琪山西师范大学食品科学学院山西临汾040000

为促进油棕蛋白在食品中的应用,本试验对油棕谷蛋白-2的营养价值和功能特性展开研究。首先采用逐步提取法从脱脂油棕中提取谷蛋白-2,测定其氨基酸组成、体外消化吸收率、生物价、乳化性和黏度。结果表明,脱脂油棕谷蛋白-2中必需氨基酸... 详细信息

为促进油棕蛋白在食品中的应用,本试验对油棕谷蛋白-2的营养价值和功能特性展开研究。首先采用逐步提取法从脱脂油棕中提取谷蛋白-2,测定其氨基酸组成、体外消化吸收率、生物价、乳化性和黏度。结果表明,脱脂油棕谷蛋白-2中必需氨基酸总含量(48.65 g/100 g)显著(P<0.05)高于FAO/WHO的推荐值(12.7 g/100 g),其含硫氨基酸(半胱氨酸+甲硫氨酸)、支链氨基酸(亮氨酸、异亮氨酸)和天冬酰胺的含量也较高。此外,脱脂油棕谷蛋白-2的生物价为63.03,体外消化吸收率为70.02 g/100 g,因此其营养价值较高。Bigelow参数分析表明,脱脂油棕谷蛋白-2中极性氨基酸与非极性氨基酸的比值为2.18,表面疏水性为37.29。SDS-PAGE分析表明,脱脂油棕谷蛋白-2的分子量为22.1~133.5 kDa,主要由5个亚基组成,且亚基之间有二硫键。功能特性分析表明,脱脂油棕谷蛋白-2的乳化性(251.58±5.28)m2/g和乳化稳定性(84.40%)很高,显著(P<0.05)高于大豆分离蛋白(32.57 m2/g);其泡沫稳定性(17.61%)和黏度(21.33 cP)与大豆分离蛋白接近。本试验结果表明脱脂油棕谷蛋白-2的营养价值较高、功能特性较好,具有开发为营养强化剂、乳化剂、增稠剂等产品的潜力。

关键词：脱脂油棕谷蛋白-2 逐步提取法营养价值功能特性

Single-cell technologies: current and near future

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Science China Life Sciences 2025年第01期 1-4页

作者： Chenfei Wang qi Liu Xiaohui Fan Tieliu shi Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration Ministry of EducationOrthopedics DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Key Laboratory of Advanced Theory and Application in Statistics and Data Science (MOE) School of StatisticsEast China Normal University

Single-cell technologies enable the indepth exploration of multiple biological hierarchies at the scale of individual cells,which have deepened our knowledge of cellular diversity, tissue organization, and overall organism function(Sun et al.,2024). In 2009, single-cell RNA sequencing(sc RNA-seq) was first developed as a powerful tool to dissect gene expression and uncover transcriptional *** the rapid advancement of technology, single-cell approaches have expanded to encompass other omics, such as genomics, epigenomics, proteomics,and metabolomics.

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Flexible molecules dedicate to release strain of inverted inorganic perovskite solar cell

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Journal of Energy Chemistry 2025年第1期100卷 87-93页

作者： Hongrui Sun Sanlong Wang Pengyang Wang Yali Liu Shanshan qi Biao shi Ying Zhao Xiaodan Zhang Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage Center State Key Laboratory of Photovoltaic Materials and CellsNankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Haihe Laboratory of Sustainable Chemical Transformations Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

The tensile strain in inorganic perovskite films induced by thermal annealing is one of the primary factors contributing to the inefficiency and instability of inorganic perovskite solar cells （IPSCs）,which reduces the defect formation ***,a flexible molecule 5-maleimidovaleric acid （5-MVA） was introduced as a strain buffer to release the residual strain of *** anhydride and carboxyl groups in 5-MVA interact strongly with the uncoordinated Pb2+through Lewis acid-base reaction,thus tightly“pull”the perovskite *** in-between soft carbon chain increased the structural flexibility of CsPbI2.85Br0.15perovskite materials,which effectively relieved the intrinsic internal strain of CsPbI2.85Br0.15,resisted the corrosion of external strain,and also reduced the formation of defects such as VIand *** addition,the introduction of 5-MVA improved crystal quality,passivated residual defects,and narrowed energy level ***,power conversion efficiency （PCE） of NiOxbased inverted IPSCs increased from 19.25% to 20.82% with the open-circuit voltage enhanced from 1.164 V to 1.230 *** release of strain also improved the stability of CsPbI2.85Br0.15perovskite films and devices.

关键词： Inverted inorganic perovskite solar cells Flexible molecules Strain release Crystallization Energy barrier High PCE

A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： qi Liu Jianye Xie Runxue Zhou Jin Deng Weihong Nie Shuwei Sun Haiping Wang Chunying shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel

Staged dendrite suppression for high safe and stable lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun Jiang Zhenjie Lu Yanwen Ding Shujun Liu Zhijie qi Tian Tang Yunfan Zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao Zhang pan Xiong Xin Wang Junwu Zhu Yongsheng Fu Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education Nanjing University of Science and Technology Jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Lithium-sulfur batteries Staged dendrite suppression Shuttle effect Surface-negatively-charged nanodiamond

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甘草超滤液中甘草酸的络合萃取研究

食品工业科技 2019年第6期40卷 157-160,166页

作者：蒲红利周博魏舒畅石盘琪孙晓燕甘肃中医药大学药学院甘肃兰州730000

以甘草酸的单次萃取率及甘草酸与甘草苷的分离率为指标,以0.2 g/mL的甘草超滤液为研究对象,在单因素实验基础上,应用析因实验考察络合萃取剂组成变化对甘草超滤液中甘草酸萃取效果的影响,优选出适合甘草酸分离的络合萃取剂及方法。结果... 详细信息

以甘草酸的单次萃取率及甘草酸与甘草苷的分离率为指标,以0.2 g/mL的甘草超滤液为研究对象,在单因素实验基础上,应用析因实验考察络合萃取剂组成变化对甘草超滤液中甘草酸萃取效果的影响,优选出适合甘草酸分离的络合萃取剂及方法。结果表明:15%三烷基氧磷(TRPO)+85%磺化煤油对甘草超滤液中甘草苷的单次萃取率为99.59%,甘草苷与甘草酸的分离率为91.31%; 6%TRPO+50%磷酸三丁酯(TBP)+44%磺化煤油对甘草酸的单次萃取率为32.98%±0.04%。得到先用15%TRPO+85%磺化煤油络合萃取超滤液中的甘草苷,再以6%TRPO+50%TBP+44%磺化煤油为萃取剂,萃取萃余水相中甘草酸的两步络合萃取法。该两步络合萃取法既能得到甘草酸,同时还可收集到副产品甘草苷。

关键词：络合萃取甘草超滤液甘草酸三烷基氧磷磷酸三丁酯磺化煤油

草酸预处理AM50镁合金表面快速原位制备蒸汽Mg−Al LDH涂层的耐蚀性

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1843-1863页

作者：潘仕琪王金梦张芬解浩峰崔蓝月 MBOBBY KANNAN 邹玉红曾荣昌山东科技大学材料科学与工程学院轻金属腐蚀实验室青岛266590 有研工程技术研究院有限公司有色金属材料制备加工国家重点实验室北京100088 School of Engineering University of NewcastleCallaghanNew South Wales2308Australia 山东科技大学化学与环境工程学院青岛266590 郑州大学材料科学与工程学院郑州450002

通过暴露合金表面的金属间化合物(β-Mg17Al12和AlMn),研究草酸(OA)酸洗对AM50镁合金表面原位蒸汽Mg−Al层状双氢氧化物(LDH)涂层成膜机制和耐蚀性的影响。结果表明,OA预处理使镁合金表面金属间化合物比例显著增加,Mg−Al LDH在Al−Mn相周... 详细信息

通过暴露合金表面的金属间化合物(β-Mg17Al12和AlMn),研究草酸(OA)酸洗对AM50镁合金表面原位蒸汽Mg−Al层状双氢氧化物(LDH)涂层成膜机制和耐蚀性的影响。结果表明,OA预处理使镁合金表面金属间化合物比例显著增加,Mg−Al LDH在Al−Mn相周围成核并生长。当酸洗时间为45 s时,所得LDH涂层的厚度最大、耐蚀性最佳,与基体相比,其腐蚀电流密度降低3个数量级。最后,提出了AM50镁合金表面Mg−Al LDH涂层的成膜机制。

关键词：镁合金草酸金属间化合物蒸汽涂层层状双氢氧化物耐蚀性

基于超滤-络合萃取及反萃取技术的甘草苷制备工艺研究

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中草药 2019年第5期50卷 1095-1099页

作者：周博蒲红利王继龙孙晓燕魏舒畅石盘棋甘肃中医药大学甘肃省中药制药工艺工程研究中心甘肃兰州730000

目的建立一种分离纯化甘草超滤液中甘草苷的络合萃取及反萃取制备技术。方法以甘草苷萃取率为指标,采用均匀设计确定最佳的络合萃取剂组成,再采用正交试验优选络合萃取工艺条件。以甘草苷反萃取率为指标,通过考察反萃取剂的种类及浓度,... 详细信息

目的建立一种分离纯化甘草超滤液中甘草苷的络合萃取及反萃取制备技术。方法以甘草苷萃取率为指标,采用均匀设计确定最佳的络合萃取剂组成,再采用正交试验优选络合萃取工艺条件。以甘草苷反萃取率为指标,通过考察反萃取剂的种类及浓度,确定反萃取甘草苷的工艺条件。结果经络合萃取研究发现,络合萃取剂宜选用由三烷基氧化膦(TRPO)和磺化煤油组成的二元络合萃取剂。甘草苷的最佳络合萃取工艺条件为TRPO-磺化煤油(9∶91)、甘草超滤液pH值调至4、有机相与水相体积比为1∶1,甘草苷平均萃取率达到99.6%。反萃取工艺研究发现,在有机相与反萃取剂体积比为1∶1的条件下,17.5mmol/LNa OH水溶液为最佳反萃取剂,甘草苷的反萃取率为99.3%。结论在优选所得条件下,甘草超滤液中的甘草苷可实现从超滤液到络合萃取剂再到碱性反萃取剂的顺利转移,甘草苷总转移率高达98.9%,可为甘草苷的分离纯化提供一种全新的制备技术。

关键词：络合萃取反萃取甘草苷三烷基氧化膦(TRPO) 甘草超滤液

重组毒素rCCK_(96-104)PE38靶向结肠癌生物特性研究

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生物技术通报 2017年第4期33卷 247-252页

作者：张嵩柳溪林李萌常江高世奇胡盼柳增善吉林大学动物医学学院人兽共患病研究所教育部重点实验室长春130062 吉林大学中日联谊医院长春130033

旨在原核表达并纯化新型重组毒素rCCK_(96-104)PE38,验证其对结肠癌细胞的靶向杀伤作用。使用基因扩增技术将反向编译的胆囊收缩素CCK_(96-104)与绿脓杆菌外毒素PE38融合,构建原核表达载体。利用Ni-NTA亲和层析进行纯化。通过结肠癌细... 详细信息

旨在原核表达并纯化新型重组毒素rCCK_(96-104)PE38,验证其对结肠癌细胞的靶向杀伤作用。使用基因扩增技术将反向编译的胆囊收缩素CCK_(96-104)与绿脓杆菌外毒素PE38融合,构建原核表达载体。利用Ni-NTA亲和层析进行纯化。通过结肠癌细胞体外杀伤实验以及结肠癌裸鼠模型的治疗验证rCCK_(96-104)PE38的抗肿瘤能力。结果显示,扩增出的rCCK_(96-104)PE38序列正确,成功利用pET-28a原核表达系统实现了活性表达。纯化后的重组蛋白能够在体外诱导结肠癌细胞凋亡,并能抑制裸鼠模型体内的肿瘤生长。成功制备高纯度、无标签的rCCK_(96-104)PE38重组免疫毒素,体内、体外实验证实其具有抑制结肠癌的能力。

关键词：重组毒素结肠癌胆囊收缩素绿脓杆菌外毒素