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牛活体采卵和体外胚胎生产技术的应用现状和展望

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浙江大学学报（农业与生命科学版） 2022年第5期48卷 557-565,604页

作者：邱金龙史延李爽张坤浙江大学动物科学学院哺乳动物分子胚胎学实验室杭州310058

活体采卵(ovum pick-up,OPU)结合体外胚胎生产(in vitro embryo production,IVP)技术能显著提高牛的繁殖效率,对奶牛和肉牛的良种扩繁具有重要意义。近年来,OPU/IVP技术在世界范围内迅速推广,逐步取代体内胚胎生产技术成为胚胎移植的主... 详细信息

活体采卵(ovum pick-up,OPU)结合体外胚胎生产(in vitro embryo production,IVP)技术能显著提高牛的繁殖效率,对奶牛和肉牛的良种扩繁具有重要意义。近年来,OPU/IVP技术在世界范围内迅速推广,逐步取代体内胚胎生产技术成为胚胎移植的主要技术手段。但是,因其整体效率低下、操作技术难度较大等,该技术体系在我国尚处于起步阶段,是制约我国奶牛和肉牛遗传改良的重要因素。本文从OPU/IVP的发展现状入手,阐述了OPU/IVP技术的进展情况,并针对该技术在良种扩繁等方面的应用前景提出展望,旨在为完善我国OPU/IVP技术体系和提高牛繁殖生产效率提供参考,从而促进养牛业的发展。

关键词：奶牛肉牛活体采卵体外胚胎生产良种扩繁

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Exploring the mechanisms of Yinlai Decoction intervening the mice model of FM1 virus infected compound with high-fat and protein-diet

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Journal of Traditional Chinese Medical Sciences 2017年第4期4卷 350-356页

作者： Shang Wang He Yu Jin feng shi Yu Hao Tiegan Liu Chen Bai Jun Wu Zeji qiu Xiaohong Gu Beijing University of Chinese Medicine Beijing 100029China Hubei University of Chinese Medicine 188 TanHualinWuchang DistrictWuhan 430061HubeiChina Zhengzhou No.2 Hospital of Chinese Medicine Zhengzhou450000HenanChina

Objective:To explore the mechanism of Yinlai Decoction (YD) intervening the mice model of FM1 influenza virus compound with high-fat and protein-diet by detecting the survival rate,lung injury,the expression of inflammatory ***:A high-fat and protein-diet mouse model was *** were randomly divided to groups of blank control,normal diet,high-fat and protein-diet (HPD),the Ribavirin,the Shuanghuanglian,the Xiaoer huashi Pill and the YD,all infected with FM1 virus via nose after four days' high-fat and *** electrogastrography (EGG) was recorded,as well as the serum levels of the gastrin and plasma vasoactive intestinal peptide (VIP) were *** in the blank control group were shame-infected with 0.05 mL saline,while the mice in other groups were infected by 0.05 mL mouse lung-adapted FM1 *** were performed by stomach perfusion for next seven *** dose saline was given in the blank control,normal diet and HPD *** life protection and mean survival days were *** lung injuries were observed by H&rE *** expression of gastrin and VIP were detected by *** serum levels of IFN-γ,IL-4,IL-6,IL-10 and TNF-α were measured by ***:Compared with normal diet group,EGG dominant frequency,EGG swing,instability coefficient increased in HPD *** with HPD group,Ribavirin group and YD group showed significantly higher survival rate and IL-10 and IFN-γ levels significantly increased while the IL-6 and TNF-α levels significantly *** with blank control group,severe lung cirrhosis injuries were found in HPD group,and mild injuries were found in YD group and Ribavirin ***:The YD can regulate the level of cytokines,and improve the pathological damages of lung tissue,and then protect the mice with high-fat and protein-diet from being injured from the FM1 virus.

关键词： Influenza virus High-fat and protein-diet Yinlai Decoction Cytokines

Effects of processing pH stimulation on cooperative bioleaching of chalcopyrite concentrate by free and attached cells

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Transactions of Nonferrous Metals Society of China 2016年第8期26卷 2220-2229页

作者： Tang-jian PENG Li-juan shi Run-lan YU Guo-hua GU Dan ZHOU Miao CHEN Guan-zhou qiu Wei-min ZENG School of Minerals Processing and Bioengineering Central South University Changsha 410083 China Key Laboratory of Biometallurgy Ministry of Education Central South University Changsha 410083 China

In order to investigate the effects of processing pH stimulation on bioleaching of chalcopyrite by moderate thermophiles,copper leaching rates and the dynamics of microbial community structures of free and attached cells were monitored. The results indicated that when the processing pH values were respectively adjusted to 1.0 and 3.0 on day 14, both free and attached cells experienced an adaptive phase. Meanwhile, the copper leaching rates were 86.9% and 64.0%,respectively, as opposed to a copper leaching rate of 87.5% in the control group without pH stimulation. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis suggested that pH stimulation imposed less impact on the attached organisms than on the free cells, indicating that the attached cells were more resistant to processing pH stimulation than the free cells. Furthermore, adjusting processing pH to 3.0 significantly disrupted both free and attached microbial communities, and the bioleaching system could not recover to the normal status as the control group.

关键词： chalcopyrite processing pH stimulation free cells attached cells microbial community real-time quantitative PCR

Prolonged Alzheimer-like Tau Hyperphosphorylation Induced by Simultaneous Inhibition of Phosphoinositol-3 Kinase and Protein Kinase C in N2a cells

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 349-354页

作者： Guo-Gang XU Yan-qiu DENG shi-Jie LIU Hong-Lian LI Jian-Zhi WANG Department of Pothophysiology,Institute of Neuroscience,Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China DepartmentofPathophysiology InstituteofNeuroscienceTongjiMedicalCollegeHuazhongUniversityofScienceandTechnol

Co-injection of wortmannin（inhibitor of phosphatidylinositol-3 kinase,PI3K）and GF109203X（inhibitor of protein kinase C,PKC）into the rat brain was found to induce spatial memory deficiency andenhance tau hyperphosphorylation in the hippocampus of rat *** establish a cell model with durativeAlzheimer-like tau hyperphosphorylation in this study,we treated N2a neuroblastoma cells with wortmanninand GF109203X separately and simultaneously,and measured the glycogen synthase kinase 3（GSK-3）activity by γ-（32）P-labeling and the level of tau phosphorylation by Western *** was found that theapplication of wortmannin alone only transitorily increased the activity of GSK-3（about 1 h）and the level oftau hyperphosphorylation at Ser396/Ser404 and Ser199/Ser202 sites（no longer than 3 h）;however,a prolongedand intense activation of GSK-3（over 12 h）and enhanced tan hyperphosphorylation（about 24 h）wereobserved when these two selective kinase inhibitors were applied *** conclude that the simultaneousinhibition of PI3K and PKC can induce GSK-3 overactivation,and further strengthen and prolong the Alzheimer-like tau hyperphosphorylation in N2a cells,suggesting the establishment of a cell model with early pathologicalevents of Alzheimer’s disease.

关键词： Alzheimer’s disease(AD) tau glycogen synthase kinase 3(GSK-3) protein kinase C(PKC) phosphatidylinositol-3 kinase(PI3K) N2a cells

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海草床恢复研究进展

生态学报 2010年第9期30卷 2443-2453页

作者：李森范航清邱广龙石雅君广西科学院广西红树林研究中心北海536000 广西大学林学院南宁530004

海草床是热带和温带重要的海洋生态系统,是许多海洋动物的栖息地、生存场所和食物来源地,具有重要的生态系统服务价值。20世纪以来,全世界的海草床衰退严重,仅1993年到2003年间约有2.6×104km2消失,达到15%,开展海草床的生态恢复已迫在... 详细信息

海草床是热带和温带重要的海洋生态系统,是许多海洋动物的栖息地、生存场所和食物来源地,具有重要的生态系统服务价值。20世纪以来,全世界的海草床衰退严重,仅1993年到2003年间约有2.6×104km2消失,达到15%,开展海草床的生态恢复已迫在眉睫。总结了海草床衰退的原因及恢复的主要方法。海草床衰退的原因包括自然灾害和人类活动的影响。海草床恢复的方法包括生境恢复法、种子法和移植法。移植法是目前最常用的方法,可分为草皮法、草块法和根状茎法。比较了各方法的优缺点,即(1)生境恢复法投入少、代价低,但周期长;(2)种子法破坏小,但种子难收集、易丧失、萌发率低;(3)草块法成活率高,但对原海草床有破坏作用;(4)根状茎法节约种源,但固定困难。因此,海草床恢复的具体方法取决于种源地、移植地及恢复的目标。我国的海草研究与恢复尚处在起始阶段,移植法应是目前我国海草床恢复的首选方法。就未来我国海草恢复的关键技术和研究重点提出了建议。

关键词：海草床恢复综述

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断层封启性的研究现状与发展方向

吉林大学学报（地球科学版） 2021年第1期51卷 65-80页

作者：张丹凤方石邱善坤吉林大学地球科学学院长春130061 海相储层演化与油气富集机理教育部重点实验室(中国地质大学) 北京100083 东北亚生物演化与环境教育部重点实验室(吉林大学) 长春130026 油页岩与共生能源矿产吉林省重点实验室(吉林大学) 长春130061 大庆钻探工程公司黑龙江大庆163357

断层封启性在油气运移和成藏中至关重要。断层封启性是指断层上、下两盘之间岩性和物理性质差异引起的排替压力差,这种压力差控制流体在断层上、下盘之间的通过能力。断层封启性从运移方向上分为垂向封启和侧向封启,从隔离带性质上分为... 详细信息

断层封启性在油气运移和成藏中至关重要。断层封启性是指断层上、下两盘之间岩性和物理性质差异引起的排替压力差,这种压力差控制流体在断层上、下盘之间的通过能力。断层封启性从运移方向上分为垂向封启和侧向封启,从隔离带性质上分为断层面封启和岩性封启。封启性的控制因素有并置作用、碎裂作用、胶结作用、涂抹充填作用以及沥青质沉淀等。其研究方法有Allan图法、断层面压力法、泥岩涂抹法、声波时差法、地震速度谱识别法、逻辑信息法、非线性映射分析法、模糊数学法和连通性概率法等。目前的研究方法均为一定角度下手段单一的定量或半定量分析,因此断层封启性研究方法的突破方向将是在不同角度下的综合定量分析。

关键词：断层封启性控制因素研究方法

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滇西临沧花岗岩基冷却的热年代学分析

岩石学报 2006年第2期22卷 465-479页

作者：施小斌丘学林刘海龄储银夏斌中国科学院边缘海地质重点实验室中国科学院南海海洋研究所广州510301 中国科学院地质与地球物理研究所北京100029 中国科学院边缘海地质重点实验室中国科学院广州地球化学研究所广州510640

为利用热年代学方法重建临沧花岗岩基的冷却历史,测定了6块花岗岩样品的锆石U-Pb年龄、黑云母Rb-Sr等时线年龄、黑云母和钾长石的^(40)Ar/^(39)Ar年龄以及锆石和磷灰石的裂变径迹数据。分析表明,自结晶以来,岩基主体经历了较复杂的热演... 详细信息

为利用热年代学方法重建临沧花岗岩基的冷却历史,测定了6块花岗岩样品的锆石U-Pb年龄、黑云母Rb-Sr等时线年龄、黑云母和钾长石的^(40)Ar/^(39)Ar年龄以及锆石和磷灰石的裂变径迹数据。分析表明,自结晶以来,岩基主体经历了较复杂的热演化,在两个基本阶段即早期快速冷却阶段和晚期缓慢冷却阶段上叠加了短周期的热扰动,从中—晚三叠世到早侏罗世末,冷却速率可以达到6℃～13℃/Ma,中侏罗世冷却缓慢,晚侏罗世到早白垩世早期,岩基快速冷却,平均速率达到5℃/Ma,早白垩世晚期到喜山运动前,为缓慢冷却阶段,平均速率仅为0．5℃/Ma,喜山运动后,受印藏碰撞的影响,岩基经历了两期冷却事件,早期冷却速率为5℃～10℃/Ma,晚期即5Ma以来冷却速率明显增大,特别是近3Ma以来的冷却速率达到16℃～20℃/Ma；早白垩世侵入的岩体(如样品S57),在侵入后快速冷却,晚白垩世以后与岩基主体同步冷却；宕基东倒糜棱岩带不是形成于150Ma,而可能是早期陆陆碰撞造山时推覆冲断的产物；自晚白垩世以来,岩基地区的总剥蚀厚度可达5000m左右,其中印藏碰撞引起的剥蚀可达3500m左右。

关键词：云南临沧岩基热演化封闭温度糜棱岩同位素年龄裂变径迹

Tunable emission of cadmium-free transition metal(Cu, Mn, Ag) co-doped ZnInS/ZnS core-shell quantum dots

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Transactions of Nonferrous Metals Society of China 2018年第8期28卷 1611-1617页

作者： qiu-hang CHEN shi-liang MEI Wu YANG Wan-lu ZHANG Gui-lin ZHANG Jia-tao ZHU Rui-qian GUO Institute for Electric Light Sources Engineering Research Center of Advanced Lighting TechnologyMinistry of EducationFudan UniversityShanghai 200433China

Color tunable quantum dots（QDs） based on the Cu, Mn, Ag co-doped Zn In S core and Zn S outer-shell were synthesized by using an eco-friendly method. Core-shell doped QDs with the average size of 3.85 nm were obtained by using a one-pot synthesis followed by a hot injection with n-dodecanethiol（DDT） and oleylamine（OLA） as stabilizers in oil phase. Cu, Mn and Ag ions were introduced as single-dopant or co-dopants during the synthesis, providing an effective means to control the emission color of the QDs. The as-synthesized QDs showed photoluminescence emission ranging from green（530 nm） to near-red（613 nm）, adjusted by doping components, dopant concentration, and Zn/In ratio. Importantly, quasi-white emission has been achieved by controlling the concentration of co-doped metal ions（Mn, Cu and Ag）. The primary results demonstrated the promising potential of co-doped QDs as alternative materials for future high quality white LED applications.

关键词： zinc group quantum dots core-shell doping tunable emission white light

Smart adsorbents for CO2 capture:Making strong adsorption sites respond to visible light

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Science China Materials 2021年第2期64卷 383-392页

作者： qiu-Rong Wu Peng Tan Chen Gu Rui Zhou shi-Chao Qi Xiao-Qin Liu Yao Jiang Lin-Bing Sun State Key Laboratory of Materials-Oriented Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced MaterialsCollege of Chemical EngineeringNanjing Tech UniversityNanjing211816China

Due to the good controllability and high energy efficiency in adsorption processes,photoresponsive adsorbents are intriguing for CO2 ***,most reported photoresponsive adsorbents are designed based on weak adsorption sites,regulating CO2 adsorption through structural change or steric *** addition,ultraviolet(UV)light is commonly involved in the regulation of adsorption *** we report for the first time the smart adsorbents for CO2 capture,which makes strong adsorption sites respond to visible(Vis)*** adsorbents were fabricated by introducing primary amine and Dispersed Red 1(DR1,a kind of push-pull azobenzene that responds to Vis light rapidly)units to mesoporous silica,which act as strong adsorption sites and triggers,*** primary amine sites make the adsorbents highly selective in the adsorption of CO2 over *** light irradiation,azobenzene is in the form of trans configuration,which leads to decreased electrostatic potential of primary amines and subsequently,exposure of active sites and liberal adsorption of *** Vis-light irradiation,cis isomers are formed,which results in increased electrostatic potential of primary amines and subsequently shelter of active *** on such strong adsorption sites,the alteration of CO2 adsorption capacity can reach 40%for the adsorbent with and without Vis-light ***,the trans/cis isomerization of DR1 units can be triggered reversibly by Vis *** present smart system endows adsorbents with selective adsorption capacity and avoids the employment of UV light,which is unlikely to be achieved by conventional photoresponsive adsorbents.

关键词： CO2 adsorption strong adsorption sites selective adsorption push-pull azobenzene visible light response