检索结果-南通市图书馆

Biomedical and Environmental Sciences 2023年第1期36卷 103-108页

作者： CUI Jing MENG Yu Han WANG Zhan Wei WANG Jing shi dong fang LIU Duo Central Laboratory Changchun Normal UniversityChangchun 130032JilinChina School of Life Sciences Northeast Normal UniversityChangchun 130024JilinChina

Alzheimer’s disease is one of the most debilitating neurodegenerative diseases affecting the aging population. One of the neuropathological hallmarks of AD is an aggregation of the tau protein into neurofibrillary tangles(NFTs) in neurons and glial cells of the brain. Tau is a microtubuleassociated protein expressed mainly in neurons of the central nervous system[1].

关键词： Alzheimer tau PC12

A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第8期18卷 1936-1943页

作者： dong Xiu-mei TAO Jing LI Ting-ting ZHANG Ping ZHU Yan TANG Yu SU Rui-hong shi dong-fang College of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R.China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development College of Veterinary MedicineNortheast Agricultural University Harbin 150030 P.R.China

An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass *** serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.

关键词： Pseudomonas aeruginosa colored silica nanoparticles agglutination test serotype detection

Isolation and Purification of Plantamajoside and Acteoside from Plant Extract of Plantago asiatica L. by High Performance Centrifugal Partition Chromatography

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2009年第6期25卷 817-821页

作者： LI Li LIU Chun-ming CHEN Zhao-jie WANG Jing shi dong-fang LIU Zhi-qiang The Central Laboratory Changchun Normal University Changchun 130032 P. R. China Laboratory of New Drug Research and Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130021 P. R. China Department of Endocrinology of China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.

关键词： Plantago asiatica L Plantamajoside Acteoside HPCPC ESI-MS HPLC-DAD

Fine Particulate Matter Exposure Induces Toxicity by Regulating Nuclear Factor-κB/toll-like Receptor 4/myeloid Differentiation Primary Response Signaling Pathways in RAW264.7 Cells

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2023年第5期36卷 458-462页

作者： ZHENG Mei Zhu LU Yao LU Ting Ting QIN Peng LI Yu Qiu shi dong fang The Central Laboratory Changchun Normal UniversityChangchun 130000JilinChina College of Life Science Changchun Normal UniversityChangchun 130000JilinChina Soybean Research Institute Jilin Academy Agricultural of ScienceChangchun 130033JilinChina

Global air pollution has become a major threat to human health. Daily variations in air pollution have been linked to increased risks of cardiopulmonaryrelated morbidity and mortality. In particular, fine particulate matter(PM) with an aerodynamic diameter ≤ 2.5 μm(PM2.5), which mostly originates from coal combustion, vehicle exhaust, and open sources in Chinese cities, is detrimental to health[1].Epidemiological and experimental investigations have demonstrated that long-term exposure to PM2.5, the most dangerous particulate in the Earth’s atmosphere, is a pivotal risk factor for cardiorespiratory diseases.

关键词： pollution mostly Earth

Ultrasonic focusing and scanning with multiple waves

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2013年第1期22卷 312-317页

作者：张碧星刘冬冬师芳芳 Hefeng dong State Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences Department of Electronics and Telecommunications Norwegian University of Science and Technology

This paper presents a new focusing and scanning method which focuses multiple waves on a target. The key of the method is to control excitation pulses for each element of the transducer array. The excitation pulse on each array element is obtained by time reversing the signal received by the same element, which is generated by an imaginary source at the target. The excitation pulses from all array elements are transmitted and arrive at the target simultaneously, and focusing is achieved. The performance of the two methods is compared in numerical examples, and it is demonstrated that the proposed method achieves a satisfactory focusing and a good signal-to-noise ratio no matter where the target location is.

关键词： ultrasonic focusing and scanning multiple waves transducer array

3-喹啉/4-吡啶混合取代阳离子型卟啉化合物的设计、合成及其生物活性(英文)

在线全文

学校读者我要写书评

暂无评论

中国药科大学学报 2005年第5期36卷 393-397页

作者：陆涛 shi dong-fang SUN Dae-Kyu HAN Hai-Yong Laurence H.Hurley 中国药科大学基础部南京210009中国College of Pharmacy and Arizona Cancer CenterUniversity of ArizonaTucson AZ85742USA College of Pharmacy and Arizona Cancer Center University of ArizonaTucson AZ85742USA

目的:寻找具有阳离子型卟啉结构的强效端粒酶抑制剂。方法:采用Adler-Longo卟啉合成法通过芳香醛和吡咯的缩合反应制得各种组合的3-喹啉/4-吡啶基卟啉碱基,再经甲基化、离子交换得阳离子型卟啉化合物。测试所合成化合物的端粒酶抑制活性... 详细信息

目的:寻找具有阳离子型卟啉结构的强效端粒酶抑制剂。方法:采用Adler-Longo卟啉合成法通过芳香醛和吡咯的缩合反应制得各种组合的3-喹啉/4-吡啶基卟啉碱基,再经甲基化、离子交换得阳离子型卟啉化合物。测试所合成化合物的端粒酶抑制活性和c-Myc抑制活性。结果:非细胞试验显示所合成的化合物具有强的端粒酶抑制活性;而化合物4显示最强的c-Myc抑制活性。结论:阳离子型卟啉化合物是潜在的抗肿瘤候选化合物。

关键词：端粒酶端粒酶抑制剂合成 G-四链体卟啉 c-Myc抑制活性

在线全文

学校读者我要写书评

暂无评论

管窥儿童图画书的后现代主义特征

编辑之友 2015年第3期 34-37页

作者：李锋史东芳大庆师范学院教师教育学院

儿童图画书已与后现代主义建立了特殊的近亲关系,解构了现代主义文本的叙事方式。文章研究梳理近10年具有后现代主义叙事风格的新派儿童图画书,按照文本、读者、权利、跨界4个维度管窥,探寻儿童图画书中的后现代主义特征,以应对后现代... 详细信息

儿童图画书已与后现代主义建立了特殊的近亲关系,解构了现代主义文本的叙事方式。文章研究梳理近10年具有后现代主义叙事风格的新派儿童图画书,按照文本、读者、权利、跨界4个维度管窥,探寻儿童图画书中的后现代主义特征,以应对后现代给儿童图画书阅读带来的挑战。

关键词：儿童图画书后现代主义特征

鸡肠炎沙门氏菌可视化交叉引物等温扩增检测技术的建立及初步应用

在线全文

学校读者我要写书评

暂无评论

中国预防兽医学报 2021年第11期43卷 1171-1177页

作者：丛秋实师东方东北农业大学动物医学学院黑龙江哈尔滨150030

为了建立一种快速检测鸡肠炎沙门氏菌(***)的新型可视化交叉等温扩增(CPA)检测技术,本研究针对鸡肠炎沙门氏菌sefA保守基因设计CPA的特异性引物,通过对反应条件和主要组分优化后建立检测***的快速检测方法。利用该CPA法对8株鸡肠炎沙门... 详细信息

为了建立一种快速检测鸡肠炎沙门氏菌(***)的新型可视化交叉等温扩增(CPA)检测技术,本研究针对鸡肠炎沙门氏菌sefA保守基因设计CPA的特异性引物,通过对反应条件和主要组分优化后建立检测***的快速检测方法。利用该CPA法对8株鸡肠炎沙门氏菌和7株其他参考菌株进行检测,同时利用环介导等温扩增(LAMP)法进行检测,对比分析本研究建立CPA法的特异性,结果显示,建立并优化基于sefA保守基因的可视化CPA法,与LAMP方法相比,该方法能够检测所有***菌株而对其他致病菌株无扩增;挑取鸡肠炎沙门氏菌ATCC 13076单菌落接种到营养肉汤培养基中过夜培养后,经菌落计数后并对菌液10倍倍比稀释(1×10^(6)cfu/mL~1×10^(-1)cfu/mL)进行敏感性试验,结果显示,CPA法的检测下限为1×10^(1)cfu/mL,为PCR法敏感性的100倍。进一步利用传统培养法对138份肉鸡腹泻粪便样品进行检测,同时利用以上CPA法、LAMP和PCR方法进行检测,评价以上各方法的检测性能,结果显示,CPA法的检出率(16.67%)与传统细菌培养法相当,但高于LAMP法(15.21%)和PCR法(13.77%),表明建立的可视化CPA法可实现快速、准确地检测鸡肠炎沙门氏菌,且不需要复杂仪器设备。本研究建立的检测鸡肠炎沙门氏菌可视化CPA法在基层兽医检验领域具有广阔的应用前景。

关键词：鸡肠炎沙门氏菌交叉等温扩增可视化敏感性临床样品快速检测

在线全文

学校读者我要写书评

暂无评论

职前教师实践性知识生成中教学意象的模塑

陕西师范大学学报（哲学社会科学版） 2016年第3期45卷 164-170页

作者：史东芳高有才大庆师范学院教师教育学院黑龙江大庆163712

教学意象统整了教师的经验、理论知识以及情感、价值、需求和信念,赋予教学应然的形象,并引导其教学行为。它反映了自我对教育教学的认识,表征着自我对教育教学行为的取向,也潜在地影响着自我的教育教学实践。让教师尤其是职前教师经过... 详细信息

教学意象统整了教师的经验、理论知识以及情感、价值、需求和信念,赋予教学应然的形象,并引导其教学行为。它反映了自我对教育教学的认识,表征着自我对教育教学行为的取向,也潜在地影响着自我的教育教学实践。让教师尤其是职前教师经过实践的浸润,进而像专家一样思考,教学意象的模塑是促进其实践性知识生成的重要途径。通过对职前教师基于情境学习的共情训练、基于课堂设计的课堂想象、基于故事言说的意象表征和基于自我反思的意象对话等几种途径进行教学意象的模塑,可以有效地促进其实践性知识的生成。

关键词：职前教师实践性知识教学意象模塑

The Application of PLC in the Overhead Crane

在线全文

学校读者我要写书评

暂无评论

Journal of Northeast Agricultural University(English Edition) 2003年第1期10卷 69-71页

作者： CUI Tian shi,dong Gui ju,fang Jun long (Northeast Agricultural University,Harbin,Heilongjiang,150030,PRC) Northeast Agricultural University HarbinHeilongjiang150030PRC

This paper is about the application of PLC (Model S5 135U made by Siemens) in the overhead *** is mainly about the working principles and application of PLC Model S5 135U.

关键词： PLC APPLICATION Overhead crane