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Modulation mechanism of aquaporins by mercury revealed by solid-state NMR and molecular dynamics simulations

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Magnetic Resonance Letters 2023年第1期3卷 85-86,I0004页

作者： shenlin wang East China University of Science and Technology Shanghai200037China

In a recent paper,the structural basis of mercury-mediated changes in aquaporins'(AQPs)function has been revealed at the atomic level by solid-state nuclear magnetic resonance(ssNMR)spectroscopy and molecular dynamics(MD)simulations[1].This work has demonstrated an interesting case of metal ion-protein *** provided valuable insights on how mercury ions achieve opposite regulations of AQP permeability by disrupting the hydrogen bonding network around arginine residues(https://***/doi/10.1021/jacs.2c10240).

关键词： interaction NMR mercury

A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain

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Synthetic and Systems Biotechnology 2023年第2期8卷 213-219页

作者： Yuting Shuai Yi Ju Yuanhang Li Dini Ma Lan Jiang Jingyu Zhang Gao-Yi Tan Xueting Liu shenlin wang Lixin Zhang Guang Liu State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic&Developmental SciencesSchool of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240China

Nucleic acid detection plays a key role in diverse diagnosis and disease *** available nucleic acid detection techniques are challenged by trade-offs among speed,simplicity,precision and ***,we described a novel method,designated SENSOR(Sulfur DNA mediated nucleic acid sensing platform),for rapid nucleic acid *** was developed from phosphorothioate(PT)-DNA and sulfur binding domain(SBD)which specifically binds double-stranded PT-modified *** utilizes PT-DNA oligo and SBD as targeting module,which is linked with split luciferase reporter to generate luminescence signal within 10 *** tested detection on synthesized nucleic acid and COVID-19 pseudovirus,achieving attomolar sensitivity combined with an amplification *** nucleotide polymorphisms(SNP)could also be *** SENSOR a new promising nucleic acid detection technique.

关键词： Phosphorothioate Sulfur binding domain Nucleic acid detection

Membrane binding of the p H sensitive antimicrobial peptide LAH4 studied by EPR spectroscopy

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Journal of Chinese Pharmaceutical Sciences 2019年第11期28卷 749-759页

作者： Yina Sheng shenlin wang Guoquan Liu State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking University Health Science CenterBeijing 100191China College of Chemistry and Molecular Engineering Peking UniversityBeijing 100871China

Peptide-membrane binding is vital for many biological events,including the bacteria combating by antimicrobial *** the p H sensitive LAH4 peptide as model,we employed a convenient electron paramagnetic resonance(EPR)method to study the peptide-membrane binding process in artificial phospholipid *** on spectral changes of the nitroxide radical labeled to the peptides,we characterized binding kinetics and affinity of peptides to different phospholipid *** binding affinity of LAH4 towards POPG was more than an order of magnitude higher than those towards DMPC and *** binding kinetics showed that LAH4 initially bound to POPG much more quickly than to DMPC and ***,p H also affected the binding kinetics in LAH4-membrane interactions,which helped explain the p H dependent antimicrobial activity of *** method might be further used to monitor the membrane binding/cell penetration of antimicrobial peptide in living cells.

关键词： EPR AMPs Peptide-membrane interaction

The Influence of Built Environment on the Spatial Distribution of Housing Price:Based on Multiple Big Data and Hedonic Model

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Journal of Landscape Research 2021年第4期13卷 47-50,56页

作者： LIU Yating wang Li LIN Yichen wang Jianghui wang shenlin School of Civil Engineering and Architecture Wuhan Institute of TechnologyWuhanHubei 4300732China Wuhan Jiankai General Contracting Co. Ltd.WuhanHubei 430000China

In recent years,more and more researches focus on the self characteristics and spatial location of housing,and explore the influencing factors of urban housing price from the micro *** representative of big cities,spatial distribution pattern of housing price in national central cities has attracted much *** order to return the spatial distribution pattern of housing price to the research on influencing factors of housing price,the reasons behind the spatial distribution pattern of housing price in three national central cities:Beijing,Wuhan and Chongqing are *** results show that①urban housing price is affected by many *** to different social and economic conditions in each city,there are differences in the influence direction of the proximity to expressways,city squares,universities and living facilities,characteristics of companies and enterprises on Beijing,Wuhan and Chongqing.②Various factors have different value-added effects on housing price in different *** location of ring line in Beijing and Wuhan has the greatest increase effect on housing price,while metro station of Chongqing has the greatest increase effect on housing price.

关键词： Big data technology National central cities Housing price Hedonic price model

OPENING UP A NEW SITUATION FOR PROMOTING FOREIGN TRADE

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China's Foreign Trade 1996年第8期 10-11页

作者： wang shenlin,Chairman of CCPIT AnhuiSub-council and CCOIC Anhui Chamberof Commerce

1996 marks the beginning of China’sNinth Five-Year Plan *** isalso a year in which Anhui Provincewill lay the foundation for carrying out itsthree major tasks—developing Anhui throughthe development of science and education,promoting export-oriented production andmaintaining sustainable development so asto attain the great goal set for the next *** CCPIT Anhui Sub-council willfirmly seize this historic opportunity to furtherstrengthen its role and functions,promoteservice quality,and extend the dimensions ofits service,providing an all round andmultifunctional service for provincial foreigneconomic and trade operations and promotingthe smooth gearing up of the province’seconomy with the world economic community. The CCPIT Anhui sub-council wasfounded in *** it has a membership of1600,including 1248 enterprise members.

关键词：韶山

生物燃料颗粒物暴露对大鼠肺泡巨噬细胞的络氨酸激酶抑制剂受体和肺表面活性蛋白D表达的影响

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中华结核和呼吸杂志 2021年第12期44卷 1090-1096页

作者：王慎临李冰冉丕鑫西北民族大学附属第一医院宁夏回族自治区人民医院银川750021 广州医科大学生命科学院 510120 广州医科大学呼吸疾病国家重点实验室广州呼吸健康研究所510120

目的观察生物燃料颗粒物(BMF)对肺泡巨噬细胞(AM)的络氨酸激酶抑制剂受体(SIRPα)、肺表面活性蛋白(SP)A和D的表达及吞噬功能的影响。方法将72只雄性SD大鼠使用随机数字表分为BMF组和清洁空气组,通过蛋白印迹法和免疫荧光检测BMF暴露4 d... 详细信息

目的观察生物燃料颗粒物(BMF)对肺泡巨噬细胞(AM)的络氨酸激酶抑制剂受体(SIRPα)、肺表面活性蛋白(SP)A和D的表达及吞噬功能的影响。方法将72只雄性SD大鼠使用随机数字表分为BMF组和清洁空气组,通过蛋白印迹法和免疫荧光检测BMF暴露4 d、1及6个月大鼠AM的SIRPα、SPA和SPD的表达,采用荧光标记金黄色葡萄糖球菌和铜绿假单胞菌,检测三个时间点大鼠AM吞噬细菌的能力。结果 BMF暴露4 d,SIRPα和SPD的蛋白与清洁空气组相比,差异无统计学意义(P>0.05);BMF暴露1个月SIRPα和SPD的蛋白相对表达量为(1.73±0.64)和(2.01±0.78),BMF暴露6个月SIRPα和SPD的蛋白相对表达量为(1.49±0.28)和(1.48±0.34),均高于清洁空气组(P<0.05)。BMF暴露1个月大鼠AM吞噬金黄色葡萄球菌和铜绿假单胞菌的MFI相对比为(0.56±0.16)和(0.80±0.09),BMF暴露6个月大鼠AM吞噬金黄色葡萄球菌和铜绿假单胞菌的MFI相对比为(0.67±0.11)和(0.76±0.16),均低于清洁空气组(P<0.05)。结论 BMF诱导AM的SIRPα和SPD的表达上调,抑制AM的吞噬功能。

关键词：生物燃料颗粒物肺泡巨噬细胞络氨酸激酶抑制剂受体吞噬功能

Characterization of Base-pairing of RNA in RNA Gels Formed by Nucleotide Repeat Expansion by Proton-detected Solid-state NMR

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Characterization of Base-pairing of RNA in RNA Gels Formed b...

2021年全国生物相分离和相变学术研讨会

作者： Sha Zhao Ziyang Wen Mengbing Zou shenlin wang College of Chemistry and Molecular Engineering Peking University Beijing NMR Center Peking University Beijing National Laboratory for Molecular Sciences State Key Laboratory of Bioreactor Engineering East China University of Science and Technology

Hexanucleotide repeat expansions of C9 orf72,(GGGGCC)n, are related to neurological diseases,including amyotrophic lateral sclerosis(ALS) and frontotemporal dementia(FTD).Previous study showed that C9 orf72 hexanucleotide repeat undergoes a soluble to gel transition in vitro without protein *** of G-quadruplex was believed to be the main driving force of the phase transition involving the GGGGCC ***,observation of the secondary structure of(GGGGCC)n in RNA gels is still *** the key structural components would provide valuable insights into the mechanisms of RNA phase separation and clues in drug ***-state NMR is powerful in characterization of bio-macromolecules within the condensed-phase ***,we report our recent advancements of novel 2 D proton-detected SSNMR experiments to analyze the base-pairings of RNA in *** on(GGGGCC)s repeats,our results revealed that both the G-C Watson-Crick base pairs and the G-G base pairs exist in(GGGGCC)s RNA gels in the presence of *** contrast,the G-quadruplex was not observed in this *** observation is different from the early solution NMR studies,which showed major G-quadruplex topology in Kcontaining *** suggests structural rearrangements of(GGGGCC) in forming RNA gels from monomers or low number of *** data,our results provide the first direct structural evidence on RNA in insoluble gel-like state,facilitating in understanding the key driving forces of RNA *** approach is adaptable to other phase separation systems involving RNA molecules.

关键词： RNA Phase separation Solid-state NMR

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对流-扩散问题的特征──块中心差分法

计算数学 1999年第4期21卷 463-474页

作者：王申林孙淑英山东大学数学系

We develop the characteristic block-centered finite difference method for nonlinear convection-diffusion problems in the rectangular domain. The discrete LZnorm errors of schemes in both the approximate solution and i... 详细信息

关键词：对流扩散问题收敛性特征-差分法块中心差分法

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新鲜齿状突骨折的分型与治疗方式选择

中国脊柱脊髓杂志 2009年第9期19卷 650-655页

作者：闫明王超王圣林北京大学第三医院骨科北京市 100191

目的:总结新鲜齿状突骨折的治疗效果,探讨不同类型骨折治疗方法的选择。方法:2000年1月～2007年12月,共收治新鲜齿状突骨折患者54例。按Grauer改良的Anderson-D′Alonzo分型,ⅡA型7例,ⅡB型23例,ⅡC型8例,Ⅲ型16例。4例伴有脊髓损伤(ⅡB... 详细信息

目的:总结新鲜齿状突骨折的治疗效果,探讨不同类型骨折治疗方法的选择。方法:2000年1月～2007年12月,共收治新鲜齿状突骨折患者54例。按Grauer改良的Anderson-D′Alonzo分型,ⅡA型7例,ⅡB型23例,ⅡC型8例,Ⅲ型16例。4例伴有脊髓损伤(ⅡB型3例,ⅡC型1例)。ⅡA型、2例合并相邻椎体骨折的ⅡB型、6例ⅡC型和Ⅲ型患者采用Halo-vest外固定;16例ⅡB型采用齿状突螺钉固定,5例移位严重的ⅡB型和2例ⅡC型采用后路寰枢固定融合术。随访时间均超过24周,拍摄颈椎侧位、开口位X线片并行CT检查观察骨折愈合情况。结果:31例Halo-vest外固定治疗者中,30例在12周时获得骨折愈合,其中4例(ⅡB型1例,Ⅲ型3例)原始骨折有前移位者,在牵引复位、Halo-vest固定后发生再移位,畸形愈合,但无神经压迫表现;1例2周时复查骨折前移位,改行后路寰枢椎固定融合术后获骨性融合。16例采用齿状突螺钉固定的ⅡB型骨折患者13例骨折愈合,3例在24周时骨折仍未愈合,骨折端有明显骨质吸收;7例寰枢关节固定融合患者在12周时均获得骨性融合。4例有脊髓损伤的患者均有不同程度的功能恢复。结论:Halo-vest外固定适合治疗ⅡA型、无或轻度移位ⅡC型和Ⅲ型齿状突骨折,但原始有前移位的骨折在固定过程中容易出现再移位。可复位的ⅡB型骨折适合齿状突螺钉固定,但骨折移位大的ⅡB型和粉碎性骨折宜及早采用寰枢固定融合术。

关键词：齿状突骨折分型外固定内固定