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Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape

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Journal of Integrative Agriculture 2024年第4期23卷 1134-1149页

作者： Ping Xu Hao Li Haiyuan Li Ge Zhao shengjie Dai Xiaoyu Cui Zhenning Liu Lei Shi Xiaohua wang College of Agriculture and Forestry Science Linyi UniversityLinyi 27600China National Key Laboratory of Crop Genetic Improvement/Microelement Research Centre/Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River) Ministry of Agriculture and Rural Affairs/Huazhong Agricultural UniversityWuhan 430070China

Phosphorus(P)is essential for living plants,and P deficiency is one of the key factors limiting the yield in rapeseed production *** the most important organ for plants,root morphology traits(RMTs)play a key role in P *** investigate the genetic variability of RMT under low P availability,we dissected the genetic structure of RMTs by genome-wide association studies(GWAS),linkage mapping and candidate gene association studies(CGAS).A total of 52 suggestive loci were associated with RMTs under P stress conditions in 405 oilseed rape *** purple acid phosphatase gene BnPAP17 was found to control the lateral root number(LRN)and root dry weight(RDW)under low P *** expression of BnPAP17 was increased in shoot tissue in P-efficient cultivars compared to root tissue and P-inefficient cultivars in response to low P ***,the haplotype of BnPAP17^(Hap3)was detected for the selective breeding of P efficiency in oilseed ***-expression of the BnPAP17^(Hap3)could promote the shoot and root growth with enhanced tolerance to low P stress and organic phosphorus(Po)utilization in oilseed ***,these findings increase our understanding of the mechanisms underlying BnPAP17-mediated low P stress tolerance in oilseed rape.

关键词： genome-wide association studies(GWAS) root morphology traits(RMTs) organic phosphorus(Po) oilseed rape BnPAP17

Stable isotope in precipitation in China:A review

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Research in Cold and Arid Regions 2012年第1期4卷 83-90页

作者： YaJu Li MingJun Zhang shengjie wang ZhongQin Li FeiTeng wang College of Geography and Environment Sciences Northwest Normal University Lanzhou Gansu 730070 China State Key Laboratory of Cryospheric Seiences/Tianshan Glaciological Station Cold and Arid Regions Environmental and En- gineering Research Institute Chinese Academy of Sciences Lanzhou Gansu 730000 China

The isotope ratios in precipitation are associated with various meteorological processes and display obvious spatial and temporal distributions, and thus can be used as impo~_nt techniques in inversing atmospheric processes, tracing vapor sources, and reflect- ing the local weather and climate conditions. The composition and distribution of stable isotopes in precipitation in China are summarized and the factors that influence isotope ratios are elucidated. An overview of related research progress in China during the past several decades is presented and the prospects for future work in this subject area are described.

关键词： precipitation stable isotope spatio-temporal distribution China

基于“以通为用,寓补于通”探析早泄因机证治

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山东中医药大学学报 2024年第5期48卷 551-556页

作者：韦一邱俊峰陈文铄王胜杰邝浩陈春秀洪志明广州中医药大学第四临床医学院广东深圳518033 深圳市中医院广东深圳518033

基于《黄帝内经》“以通为用”理论,结合临床早泄的病机特点,总结出“精室失用,虚实夹杂”的核心病机。提出早泄基础病机为心肾不交,精室统摄失司;肾虚邪滞,精室开阖无度;肝郁脾虚,精室藏泄失调。“以通为用,寓补于通”是核心治法,该病... 详细信息

基于《黄帝内经》“以通为用”理论,结合临床早泄的病机特点,总结出“精室失用,虚实夹杂”的核心病机。提出早泄基础病机为心肾不交,精室统摄失司;肾虚邪滞,精室开阖无度;肝郁脾虚,精室藏泄失调。“以通为用,寓补于通”是核心治法,该病的基本治疗方法为:交通心肾,率精室统摄之权,方选桂枝加龙骨牡蛎汤合交泰丸加减;益肾祛邪,固精室开阖之枢,方选金匮肾气丸合水陆二仙丹加减;疏肝健脾,畅精室藏泄之机,方选自拟柴芍畅精方。

关键词：早泄精室失用虚实夹杂以通为用寓补于通

STUDY FOR NATURAL GAS HYDRATE CONVERSED FROM ICE

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化工学报 2003年第Z1期54卷 23-28页

作者： wang shengjie, SHEN Jiandong, HAO Miaoli and LIU Furong (School of Environmental and Chemical Engineering, Xi’an Jiaotong University, Xi’an 710049, Shaanxi, China) School of Environmental and Chemical Engineering Xi'an Jiaotong University Xi'an 710049 Shaanxi China

Natural gas hydrates are crystalline clathrate compounds composed of water and gases of small molecular diameters that can be used for storage and transport of natural gas as a novel method. In the paper a series of experiments of aspects and kinetics for hydrate formed from natural gas and ice were carried out on the industrial small scale production apparatus. The experimental results show that formation conditions of hydrate conversed from ice are independent of induction time, and bigger degrees of supersaturation and supercooling improved the driving force and advanced the hydrate *** is also favorable for ice particle conversion to hydrate. In addition, it was found there have an optimal reaction time during hydrate formation.

关键词： natural gas hydrate, formation, ice, supersuturation, supercooling, superpressure

Establishment of a rat model of severe spontaneous intracerebral hemorrhage

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Journal of Intensive Medicine 2024年第1期4卷 108-117页

作者： Shuixiang Deng shengjie Feng Yuewen Xin Yu He Yao wang Mi Tian Ye Gong Department of Critical Care Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Neurosurgery Huashan HospitalFudan UniversityShanghaiChina

Background:Severe intracerebral hemorrhage(ICH)is the most devastating subtype of stroke resulting in high mortality and *** present,the development of targeted treatments to minimize the high morbidity and mortality is limited partly due to the lack of a severe ICH animal *** this study,we aimed to establish an accurate severe ICH model in rats and examine the pathological and physiological changes associated with ***:A rat model of severe ICH model was established by intrastriatal injection of autologous blood using different blood volumes(ICH 100μL group,ICH 130μL group,ICH 160μL group,ICH 170μL group,and ICH 180μL group).The mortality was assessed during the 28-day post-ICH ***-and long-term neurological deficits were evaluated using the Longa method,foot fault,falling latency,and Morris water maze *** water content,hematoma volume,hemoglobin content,and magnetic resonance imaging were assessed to determine the extent of brain *** staining was conducted to examine microglial activation and neuronal *** and eosin(H&E)staining,lung water content,and western blotting were used to assess lung injury following ***:The mortality of ICH rats increased significantly with an increase in autologous blood *** 28-day mortality in the 100μL,130μL,160μL,170μL,and 180μL ICH groups were 5%,20%,40%,75%,and 100%,respectively.A significantly higher 28-day mortality was observed in the ICH 160μL group compared to the ICH 100μL *** ICH 160μL group exhibited significantly increased neurological deficits,brain edema,hematoma volume,and hemoglobin content compared to the sham *** with the sham operation group,the activation of microglia and neuronal death in ICH 160μL rats *** use of H&E staining and western blotting demonstrated that disruption of the intra-alveolar structure,alveolar edema,and infiltration of inflammatory cells and cytokines into the lung tissu

关键词： Severe spontaneous intracerebral hemorrhage Animal model Autologous blood injection Animal behavior Lung injury

Negative feedback regulation of PROG1 in rice

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Journal of Integrative Agriculture 2024年第9期23卷 3234-3237页

作者： Jinlin Bao Jing Huang Xiaoqing Yang Xizhi Li shengjie Cheng Wei Huang Jun wang Jian Jin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Nanning 530004China College of Life Science and Technology Guangxi UniversityNanning 530004China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China National Key Laboratory of Plant Molecular Genetics/Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences(CAS)/Institute of Plant Physiology and EcologyCASShanghai 200032China

Cultivated rice(Oryza sativa L.),one of the most widely grown and important crops worldwide,is believed to have been domesticated from the ancestral wild rice(Oryza rufipogon Griff.)thousands of years ago(Cheng et al.... 详细信息

关键词： Oryza crops Cultivated

双石通淋胶囊联合盐酸多西环素治疗男性非淋菌性尿道炎(湿热下注型)的临床研究

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中国男科学杂志 2024年第1期38卷 115-119页

作者：韦一洪志明陈子龙陈文铄王胜杰邱俊峰翁浩伟广州中医药大学广东广州510006 广州中医药大学第四临床医学院(深圳市中医院) 广东深圳518033

目的观察双石通淋胶囊联合盐酸多西环素治疗男性非淋菌性尿道炎(湿热下注型)的临床疗效及安全性。方法将2022年3月至2023年3月期间,就诊的104例非淋菌性尿道炎(湿热下注型)患者依据区组随机化分为治疗组(n=52)和对照组(n=52)。对照组给... 详细信息

目的观察双石通淋胶囊联合盐酸多西环素治疗男性非淋菌性尿道炎(湿热下注型)的临床疗效及安全性。方法将2022年3月至2023年3月期间,就诊的104例非淋菌性尿道炎(湿热下注型)患者依据区组随机化分为治疗组(n=52)和对照组(n=52)。对照组给予盐酸多西环素口服治疗7 d,治疗组则在此基础上联合双石通淋胶囊口服7 d。两组患者在停药后7 d进行复查。观察两组患者的临床疗效,并比较两组患者的中医症候评分、临床症状消退时间、病原体实验室检测结果和不良反应发生率。结果治疗组与对照组的总有效率分别为96.15%和84.62%,差异具有显著性(P0.05)。结论双石通淋胶囊联合盐酸多西环素治疗湿热下注型男性非淋菌性尿道炎可显著提高临床病原体转阴率,有效改善临床症状,提高患者生活质量。

关键词：尿道炎双石通淋胶囊多西环素湿热下注

miR-24-3p promotes proliferation and inhibits apoptosis of porcine granulosa cells by targeting P27

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Journal of Integrative Agriculture 2024年第4期23卷 1315-1328页

作者： shengjie Shi Lutong Zhang Liguang wang Huan Yuan Haowei Sun Mielie Madaniyati Chuanjiang Cai Weijun Pang Lei Gao Guiyan Chu College of Animal Science and Technology Northwest A&F UniversityYangling 712100China Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceYangling 712100China

Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and *** level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landrace sows was significantly higher than that of low-yielding ***,the functions of miR-24-3p on GCs are *** this study,using flow cytometry,5-ethynyl-2′-de-oxyuridine(EdU)staining,and cell count,we showed that miR-24-3p promoted the proliferation of GCs increasing the proportion of cells in the S phase and upregulating the expression of cell cycle genes,moreover,miR-24-3p inhibited GC ***,on-line prediction,bioinformatics analysis,a luciferase reporter assay,RT-qPCR,and Western blot results showed that the target gene of miR-24-3p in proliferation and apoptosis is cyclin-dependent kinase inhibitor 1B(P27/CDKN1B).Furthermore,the effect of miR-24-3p on GC proliferation and apoptosis was attenuated by P27 *** findings suggest that miR-24-3p regulates the physiological functions of GCs.

关键词： miR-24-3p granulosa cells proliferation apoptosis

基于虹吸效应的特高压800 kV换流变呼吸器漏油原因分析

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青海电力 2024年第S1期43卷 96-100页

作者：吴对平郭冠军王生杰包正红董生成李渊沈洁崔拼搏国网青海省电力公司电力科学研究院青海西宁810008 国网青海省电力公司超高压公司青海西宁810008

某特高压换流站800 kV换流变呼吸器在运行过程中发生漏油,停电后现场解体检查发现储油柜内部胶囊破损,开展呼吸器漏油分析和虹吸效应机理分析,确定漏油原因为胶囊破损导致内部进油,在高温大负荷期间达到虹吸效应的激发条件,导致胶囊内... 详细信息

某特高压换流站800 kV换流变呼吸器在运行过程中发生漏油,停电后现场解体检查发现储油柜内部胶囊破损,开展呼吸器漏油分析和虹吸效应机理分析,确定漏油原因为胶囊破损导致内部进油,在高温大负荷期间达到虹吸效应的激发条件,导致胶囊内部进入的油经呼吸器管路流出,并结合分析结果对后续换流变胶囊运维检修提供建议。

关键词：换流变胶囊储油柜破损虹吸效应