Establishment of a rat model of severe spontaneous intracerebral hemorrhage

作     者：Shuixiang Deng Shengjie Feng Yuewen Xin Yu He Yao Wang Mi Tian Ye Gong Shuixiang Deng;Shengjie Feng;Yuewen Xin;Yu He;Yao Wang;Mi Tian;Ye Gong

作者机构：Department of Critical Care MedicineHuashan HospitalFudan UniversityShanghaiChina Department of NeurosurgeryHuashan HospitalFudan UniversityShanghaiChina 

出 版 物：《Journal of Intensive Medicine》 (重症医学（英文）)

年 卷 期：2024年第4卷第1期

页      面：108-117页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by a grant from the Shanghai Hospital Development Center(SHDC2020CR3021A to YG) the Science and Technology Commission of Shanghai Municipality(21ZR1410700 to S.D.) the National Natural Science Foun-dation of China(82101536 to S.D.) 

主　　题：Severe spontaneous intracerebral hemorrhage Animal model Autologous blood injection Animal behavior Lung injury 

摘      要：Background:Severe intracerebral hemorrhage(ICH)is the most devastating subtype of stroke resulting in high mortality and *** present,the development of targeted treatments to minimize the high morbidity and mortality is limited partly due to the lack of a severe ICH animal *** this study,we aimed to establish an accurate severe ICH model in rats and examine the pathological and physiological changes associated with ***:A rat model of severe ICH model was established by intrastriatal injection of autologous blood using different blood volumes(ICH 100μL group,ICH 130μL group,ICH 160μL group,ICH 170μL group,and ICH 180μL group).The mortality was assessed during the 28-day post-ICH ***-and long-term neurological deficits were evaluated using the Longa method,foot fault,falling latency,and Morris water maze *** water content,hematoma volume,hemoglobin content,and magnetic resonance imaging were assessed to determine the extent of brain *** staining was conducted to examine microglial activation and neuronal *** and eosin(H&E)staining,lung water content,and western blotting were used to assess lung injury following ***:The mortality of ICH rats increased significantly with an increase in autologous blood *** 28-day mortality in the 100μL,130μL,160μL,170μL,and 180μL ICH groups were 5%,20%,40%,75%,and 100%,respectively.A significantly higher 28-day mortality was observed in the ICH 160μL group compared to the ICH 100μL *** ICH 160μL group exhibited significantly increased neurological deficits,brain edema,hematoma volume,and hemoglobin content compared to the sham *** with the sham operation group,the activation of microglia and neuronal death in ICH 160μL rats *** use of H&E staining and western blotting demonstrated that disruption of the intra-alveolar structure,alveolar edema,and infiltration of inflammatory cells and cytokines into the lung tissu