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World Journal of Gastroenterology 2009年第20期15卷 2500-2505页

作者： Ye Zong Shu-Tian Zhang sheng-tao zhu Department of Gastroenterology Beijing Friendship Hospital Affiliated to the Capital Medical University Beijing Digestive Disease Center Beijing 100050 China

AIM： To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of ***： The esophageal squamous carcinoma cell line （TE-13） was treated with different concentrations of nicotine （100 μg/mL and 200 μg/mL） or 200 μg/mL nicotine plus 100 μmol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 （MMP-2） was analyzed by zymography and ***： Nicotine （100 μg/mL, 200 μg/mL） enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine （200 μ/mL） stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2. CONCLUSION： Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.

关键词： Carcinoma Cyclooxygenase 2 inhibitors Esophagus Nicotine Squamous cell

Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2011年第37期17卷 4225-4230页

作者： Jun-Xiong Wang Yuan-Long He sheng-tao zhu Shuo Yang Shu-Tian Zhang Beijing Friendship Hospital Affiliated to the Capital Medical University Beijing 100050 China Department of Gastroenterology Qingdao Municipal Hospital Qingdao 266071 Shandong Province China Department of Gastroenterology Beijing Friendship Hospital Affiliated to the Capital Medical University Beijing 100050 China Shuo Yang Laboratory Medicine Department Peking University Third Hospital Beijing 100050 China

AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal esophageal epithelial cell line,primary ESCC tumor tissue,and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction(RTPCR).Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein *** specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate ***:In the majority of ESCC cell lines,we found that PTX3 expression was down-regulated due to gene promoter hypermethylation,which was further confirmed by bisulphite genomic *** treatment with 5-aza-2'-deoxycytidine restored PTX3 mRNA expression in ESCC cell *** was more common in tumor tissues(85%) than in adjacent nontumor tissues(25%)(P < 0.01).CONCLUSION:PTX3 is down-regulated through promoter hypermethylation in ESCC,and could potentially serve as a biomarker of ESCC.

关键词： Tumor suppressor gene Pentraxin 3 Microarray DNA methylation Esophageal squamous cell carcinoma

Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2012年第6期18卷 532-540页

作者： Xiao-Wen Hao sheng-tao zhu Yuan-Long He Peng Li Yong-Jun Wang and Shu-Tian Zhang Xiao-Wen Hao Sheng-Tao Zhu Yuan-Long He Peng Li Yong-Jun Wang Shu-Tian Zhang Department of Gastroenterology Beijing Friendship Hospital Capital Medical University 10050 Beijing China

AIM： To investigate the expression and methylation status of the secreted frizzled-related protein 2 （SFRP2） in esophageal squamous cell carcinoma （ESCC） and ex- plore its role in ESCC ***： Seven ESCC cell lines （KYSE 30, KYSE150, KYSE410, KYSE510, EC109, EC9706 and TE-1） and one immortalized human esophageal epithelial cell line （Het- 1A）, 20 ESCC tissue samples and 20 paired adjacent non-tumor esophageal epithelial tissues were analyzed in this study. Reverse-transcription polymerase chain reaction （RT-PCR） was employed to investigate the expression of SFRP2 in cell lines, primary ESCC tumor tissue, and paired adjacent normal tissue. Methylation status was evaluated by methylation-specific PCR and bisulfite sequencing. The correlation between expres- sion and promoter methylation of the SFRP2 gene was confirmed with treatment of 5-aza-2＇-deoxycytidine. To assess the potential role of SFRP2 in ESCC, we es-tablished stable SFRP2-transfected cells and examined them with regard to cell proliferation, colony formation, apoptosis and cell cycle in vivo and in ***： SFRP2 mRNA was expressed in the im- mortalized normal esophageal epithelial cell line but not in seven ESCC cell lines. By methylation-specific PCR, complete methylation was detected in three cell lines with silenced SFRP2 expression, and extensive methylation was observed in the other four ESCC cell lines. 5-aza-2＇-deoxycytidine could restore the expres- sion of SFRP2 mRNA in the three ESCC cell lines lack- ing SFRP2 expression. SFRP2 mRNA expression was obviously lower in primary ESCC tissue than in adjacent normal tissue （0.939 ± 0.398 vs 1.51 ± 0.399, P 〈 0.01）. SFRP2 methylation was higher in tumor tissue than in paired normal tissue （95% vs 65%, P 〈 0.05）. The DNA methylation status of the SFRP2 correlated inversely with the SFRP2 expression. To assess the potential role of SFRP2 in ESCC, we established stable SFRP2 transfectants and control counterparts by in- troducing pcDN

关键词： Esophageal squamous cell carcinoma Se-creted frizzled-related protein 2 Methylation Tumor sup-pressor gene Wnt signaling pathway

Association between gastric cancer and -1993 polymorphism of TBX21 gene

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World Journal of Gastroenterology 2012年第10期18卷 1117-1122页

作者： Le-Hui Zhang Qin Li Peng Li sheng-tao zhu Jing Wang Hong-Li Yang Chang-Qing Xu Xiu-Hua Guo Department of Epidemiology and Health Statistics School of Public Health and Family Medicine Capital Medical University Beijing 100069 China Department ofOncology Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of Gastroenterology Qianfoshan Hospital Shandong University Jinan 250014 Shandong Province China

AIM: To investigate the association between the polymorphism of TBX21 gene and the risk of gastric cancer in a Chinese population. METHODS: The -1993 polymorphism located in TBX21 gene promoter region was identified by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) method. The risk between TBX21 gene genotype and gastric cancer was determined by multivariate logistic regression analysis in 220 gastric cancer patients and 262 cancer-free controls matched by age, sex and ethnicity. RESULTS: Compared with the TBX21 -1993TT genotype, the -1993CC genotype exhibited a significantly elevated risk for gastric cancer [Odds ratio (OR) = 3.42, 95% confidence interval (CI): 1.41-8.31]. The relation-ship between the -1993 polymorphic genotype and the invasive status such as lymph node and distant metastasis was found among the gastric cancer patients (OR = 4.02, 95% CI: 1.87-8.66; OR = 7.02, 95% CI: 3.44-14.34, respectively). CONCLUSION: TBX21 -1993 polymorphism might contribute to the risk of gastric cancer, especially to the distant metastasis.

关键词： TBX21 gene Gastric cancer Polymorphism Genetic susceptibility Association analysis

Reliability Analysis on Data of SO_2 Emissions from Thermal Power Plants

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Electricity 2006年第4期17卷 47-49页

作者： Wang sheng tao Shenxin zhu Fahua State Power Environmental Protection Research Institute

Since the atmospheric pollutants from thermal power plants account for a large proportion of the national total, knowing well the status of SO2 emissions of power industry is of great significance for making control strategies and related environmental policies concerning SO2 and acid rain. Through introduction and analysis to some key links, such as the existing monitoring network, data sources, examining methods and procedures for statistic data and calculating methods of total national emissions, it is concluded that the data of SO2 emissions from the statistic database for power environment is reliable and can be a reference for decision-making both on power development and environmental protection.

关键词：环境保护环境污染二氧化硫能源工业

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Inhibitory Role of Pentraxin-3 in Esophageal Squamous Cell Carcinoma

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中华医学杂志(英文版) 2016年第18期129卷 2233-2240页

作者： Dan Ma Ye Zong sheng-tao zhu Yong-Jun Wang Peng Li Shu-Tian Zhang Department of Gastroenterology Beijing Friendship Hospital Capital Medical University National Clinical Research Center for Digestive Diseases Beijing Digestive Disease Center Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases

Background:Esophageal cancer is the sixth leading cause of cancer-related death ***-3(PTX3) is a member of the PTX ***,we investigated the role of PTX3 in esophageal squamous cell carcinoma(ESCC).Methods:The effect of PTX3 on ESCC cell proliferation,colony formation,apoptosis,migration,and invasion was investigated using cell viability assays,colony formation assays,flow cytometry,and migration and invasion *** effect of PTX3 on the tumorigenicity of ESCC in vivo was investigated with xenograft studies in nude ***:PTX3 overexpression in ESCC cells reduced cellular proliferation and colony formation(P < 0.05) and increased the rate of apoptosis(P < 0.05).PTX3 expression had no significant effect on the migratory or invasive potential of ESCC *** our mouse model of human ESCC,we achieved 100%successful tumor *** with the control and empty vector-expressing groups,the PTX3-expressing group formed significantly smaller tumors(P < 0.05).Conclusions:This study indicates that PTX3 might play an inhibitory role in ESCC.

关键词： Apoptosis Esophageal Squamous Cell Carcinoma Pentraxin-3 Proliferation

Challenge in the new era:Translational medicine in gastrointestinal endoscopy and early cancer

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Chronic Diseases and Translational Medicine 2019年第4期5卷 234-242页

作者： Yang Yu Yan-Hua Yin Li Min sheng-tao zhu Peng Li Shu-Tian Zhang Department of Gastroenterology Beijing Friendship HospitalCapital Medical UniversityNational Clinical Research Center for Digestive DiseaseBeijing Digestive Disease CenterBeijing Key Laboratory for Precancerous Lesion of Digestive DiseaseBeijing 100050China Department of Pathology Liaocheng People's HospitalLiaochengShandong 252000China

Translational medicine is a new medical model that has emerged over the past 20 years and is dedicated to bridging the gap between basic and clinical *** the same time,the diagnosis and treatment of digestive diseases,especially gastrointestinal endoscopy,have been rapidly *** emergence of new techniques for gastrointestinal endoscopy has changed the therapeutic spectrum of some diseases and brought huge benefits to *** therapy has positively affected the individualized and precise treatment of patients with advanced gastrointestinal *** construction of a standardized biobank provides a strong guarantee for clinicians to conduct translational medical *** medicine has brought good development opportunities,but it also faces *** training of translational medicine researchers and the transformation of educational models require sufficient attention for further development.

关键词： Translational medicine Endoscopy Targeted therapy Biobank

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Secure beamforming and deployment design for rate-splitting multiple access-based UAV communications

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Science China(Information Sciences) 2025年第1期68卷 342-356页

作者： Bing WANG Xiaofeng tao Shujun HAN Huici WU Kai YANG zhu HAN National Engineering Research Center of Mobile Network Technologies Beijing University of Posts and Telecommunications Peng Cheng Laboratory Department of Electrical and Computer Engineering University of Houston

Rate-splitting multiple access(RSMA) has recently gained attention as a potential robust multiple access(MA)scheme for upcoming wireless networks. Given its ability to efficiently utilize wireless resources and design interference management strategies, it can be applied to unmanned aerial vehicle(UAV) networks to provide convenient services for large-scale access ground users. However, due to the line-of-sight(LoS) broadcast nature of UAV transmission, information is susceptible to eavesdropping in RSMA-based UAV networks. Moreover, the superposition of signals at the receiver in such networks becomes complicated. To cope with the challenge, we propose a two-user multi-input single-output(MISO) RSMA-based UAV secure transmission framework in downlink communication networks. In a passive eavesdropping scenario, our goal is to maximize the sum secrecy rate by optimizing the transmit beamforming and deployment location of the UAV-base station(UAV-BS),while considering quality-of-service(QoS) constraints, maximum transmit power, and flight space limitations. To address the non-convexity of the proposed problem, the optimization problem is first decoupled into two subproblems. Then, the successive convex approximation(SCA) method is employed to solve each subproblem using different propositions. In addition, an alternating optimization(AO)-based location RSMA(L-RSMA) beamforming algorithm is developed to implement joint optimization to obtain the suboptimal solution. Numerical results demonstrate that(1) the proposed L-RSMA scheme yields a28.97% higher sum secrecy rate than the baseline L-space division multiple access(SDMA) scheme;(2) the proposed L-RSMA scheme improves the security performance by 42.61% compared to the L-non-orthogonal multiple access(NOMA) scheme.

关键词： rate-splitting multiple access UAV communication precoding optimization deployment location physical layer security

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糖尿病神经病变患者单核细胞TOLL样受体4的表达及其与血浆肿瘤坏死因子α、白介素6的相关性研究

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中国糖尿病杂志 2017年第2期25卷 97-102页

作者：孟庆胜朱涛南京医科大学附属南京医院(南京市第一医院)麻醉科 210029 上海市交通大学附属第一人民医院松江分院麻醉科

目的研究T2DM伴糖尿病神经病变患者外周血单核细胞TOLL样受体4(TLR4)的表达及其与促炎因子TNF-α、IL-6的相关性。方法将研究对象分为正常组(NC,n=44),T2DM组(n=44),T2DM神经病变组(n=44)。分离和提取外周血单核细胞,流式细胞术和RT-PC... 详细信息

目的研究T2DM伴糖尿病神经病变患者外周血单核细胞TOLL样受体4(TLR4)的表达及其与促炎因子TNF-α、IL-6的相关性。方法将研究对象分为正常组(NC,n=44),T2DM组(n=44),T2DM神经病变组(n=44)。分离和提取外周血单核细胞,流式细胞术和RT-PCR检测单核细胞表面TLR4蛋白和mRNA;ELISA检测血浆TNF-α和IL-6。结果 T2DM神经病变组HbA_1 c[(9.09±-1.62)%vs(8.36土1.10)%vs(5.30±0.89)%]、单核细胞TLR4蛋白[(42.02±9.69)%vs(31.27±6.87)%vs(11.96±5.54)%]和TLR4 mRNA[(2.98±1.06)vs(1.74±0.47)vs(1.12±0.52)]、血浆TNF-α[(8.75±3.14)vs(6.27土3.64)vs(3.19±1.17)Pg/m1]、IL-6[(3.63±1.81)vs(2.60±1.14)vs(1.54±0.58)pg/ml]水平均高于T2DM和NC组(P<0.01);T2DM神经病变组TLR4蛋白与TNF-α、IL-6呈正相关(r=0.631,P<0.0001;r=0.447,P=0.0023)。结论糖尿病神经病变患者单核细胞TLR4参与机体炎症反应,与糖尿病神经病变的发生发展密切相关。

关键词：糖尿病神经病变 TOLL样受体4 外周血单核细胞肿瘤坏死因子α 白介素6

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Plasmonic tandem heterojunctions enable high-efficiency charge transfer for broad spectrum photocatalytic hydrogen production

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Journal of Energy Chemistry 2025年第1期100卷 710-720页

作者： Wenxuan Chen Xiu-Qing Qiao Guo Hui Bojing Sun Dongfang Hou Meidi Wang Xueqian Wu tao Wu Dong-sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion Materials China Three Gorges University Hubei Three Gorges Laboratory College of Chemistry and Materials Science and Guangdong Provincial Key Laboratory of Supramolecular Coordination Chemistry Jinan University

Rational engineering of semiconductor photocatalysts for efficient hydrogen production is of great significance but still challenging,primarily due to the limitations in charge transfer ***,a fascinating plasmonic tandem heterojunction with the hc-CdS/Mo2C@C heterostructure is aimfully prepared for effectively promoting the charge separation kinetics of the CdS photocatalyst via the synergistic strategy of phase junction,Schottky junction,and photothermal *** difference in atomic configuration between cubic-CdS （c-CdS） and hexagonal-CdS （h-CdS） leads to effective charge separation through a typical Ⅱ charge transfer mechanism,and plasmonic Schottky junction further extracts the electrons in the hc-CdS phase junction to realize gradient charge ***,the photothermal effect of Mo2C@C helps to expand the light absorption,accelerate charge transfer kinetics,and reduce the hydrogen evolution energy *** carbon layer provides a fast channel for charge transfer and protects the photocatalyst from *** a result,the optimized hc-CMC photocatalyst exhibits a significantly high photocatalytic H2production activity of 28.63 mmol/g/h and apparent quantum efficiency of 61.8%,surpassing most of the reported *** study provides a feasible strategy to enhance the charge transfer kinetics and photocatalytic activity of CdS by constructing plasmonic tandem heterogeneous junctions.

关键词： Cadmium sulfide