Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide
Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide作者机构：Department of Gastroenterology Beijing Friendship Hospital Affiliated to the Capital Medical University Beijing Digestive Disease Center Beijing 100050 China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2009年第15卷第20期
页 面：2500-2505页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by Beijing Municipal Commission of Education, Science and Technology Program, No. KM200610025029 Beijing Municipal Natural Science Foundation, No. 7072022
主 题：Carcinoma Cyclooxygenase 2 inhibitors Esophagus Nicotine Squamous cell
摘 要：AIM： To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of ***： The esophageal squamous carcinoma cell line （TE-13） was treated with different concentrations of nicotine （100 μg/mL and 200 μg/mL） or 200 μg/mL nicotine plus 100 μmol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 （MMP-2） was analyzed by zymography and ***： Nicotine （100 μg/mL, 200 μg/mL） enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine （200 μ/mL） stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2. CONCLUSION： Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect ofnicotine-enhanced esophageal squamous carcinoma cell migration and invasion.
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