检索结果-南通市图书馆

Insight into the in vivo fate of intravenous herpetrione amorphous nanosuspensions by aggregation-caused quenching probes

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第11期33卷 4948-4951页

作者： Lingyu Hang chengying shen Baode shen Hailong Yuan Department of Pharmacy Air Force Medical CenterPLABeijing 100142China College of Pharmacy Jiangxi University of Chinese MedicineNanchang 330004China Department of Pharmacy Jiangxi Provincial People's HospitalNanchang 330006China

Intravenous nanosuspensions are attracted growing attention as a viable strategy for development of intravenous formulations of poorly water-soluble drugs.However,only few information about the biological fate of intravenous nanosuspensions is currently known,especially amorphous nanosuspensions are not reported yet.In this study,the in vivo fate of herpetrione(HPE)amorphous nanosuspensions following intravenous administration was explored by using an aggregation-caused quenching(ACQ)probe and HPLC methods.The ACQ probe is physically embedded into HPE nanoparticles via anti-solvent method to form HPE hybrid nanosuspensions(HPE-HNSs)for bioimaging.HPE-HNSs emit strong and stable fluorescence,but fluorescence quenches immediately upon the dissolution of HPE-HNSs,confirming the selfdiscrimination of HPE-HNSs.Following intravenous administration of HPE-HNSs,integral HPE-HNSs and HPE show similar degradation and biodistribution,with rapid clearance from blood circulation and obvious accumulation in liver and lung.Due to the slower dissolution and enhanced recognition by reticuloendothelial system,450 nm HPE-HNSs accumulate more in liver,lung and spleen than that of 200 nm HPE-HNSs.These results demonstrate that integral HPE-HNSs determine the in vivo performance of HPEHNSs.This study provides insight into the in vivo fate of intravenous amorphous nanosuspensions.

关键词： Amorphous nanosuspensions Herpetrione In vivo fate Intravenous delivery Aggregation-caused quenching

在线全文

学校读者我要写书评

暂无评论

黄芩汤的抗须癣毛癣菌活性及作用机制研究

中国药房 2024年第3期35卷 311-315页

作者：沈成英罗忠章佩邓冯沂申宝德胡建新江西省人民医院(南昌医学院第一附属医院)药学部南昌330006 南昌大学药学院南昌330006 江西省人民医院(南昌医学院第一附属医院)临床医学研究所南昌330006 江西中医药大学现代中药制剂教育部重点实验室南昌330004

研究黄芩汤(HQD)的抗须癣毛癣菌活性及作用机制。方法通过测定最小抑菌浓度(MIC)、最小杀菌浓度(MFC)、菌丝长度、孢子萌发率、生物量和观察菌丝超微结构评价HQD的抗须癣毛癣菌活性;通过山梨醇保护实验检测HQD对须癣毛癣菌细胞壁的影响... 详细信息

研究黄芩汤(HQD)的抗须癣毛癣菌活性及作用机制。方法通过测定最小抑菌浓度(MIC)、最小杀菌浓度(MFC)、菌丝长度、孢子萌发率、生物量和观察菌丝超微结构评价HQD的抗须癣毛癣菌活性;通过山梨醇保护实验检测HQD对须癣毛癣菌细胞壁的影响;通过测定麦角固醇含量和角鲨烯环氧酶(SE)、羊毛甾醇14α-去甲基化酶(CYP51)的活性考察HQD对须癣毛癣菌细胞膜的影响;通过测定线粒体中苹果酸脱氢酶(MDH)、琥珀酸脱氢酶(SDH)及ATP酶(包括钠钾ATP酶、钙镁ATP酶和总ATP酶)的活性考察HQD对须癣毛癣菌线粒体的影响。结果 HQD对须癣毛癣菌具有显著的抑菌活性,MIC、MFC值分别为3.13、25 mg/mL。经HQD干预后,须癣毛癣菌菌丝长度均显著缩短(P<0.05);孢子萌发率、生物量、细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体中MDH、SDH、各种ATP酶的活性均显著降低(P<0.05);细胞结构受到了一定程度的破坏,但细胞壁的完整性没有受影响。结论 HQD具有显著的抗须癣毛癣菌活性,其作用机制与降低细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体相关酶活性有关。

关键词：黄芩汤须癣毛癣菌抗真菌活性作用机制细胞膜线粒体

Effects of novel Fufang Biejia Ruangan Tablets with sheep placenta as substitute for Hominis Placenta on CCl4-induced liver fibrosis

在线全文

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2022年第1期14卷 104-110页

作者： Baode shen Li Deng Yuan Liu Ruisheng Li chengying shen Xiao Liu Yinchao Li Hailong Yuan Department of Pharmacy Air Force Medical CenterPLABeijing 100142China College of Pharmaceutical Sciences Zhengzhou UniversityZhengzhou 450001China Research Center for Clinical and Translational Medicine The Fifth Medical Center of ChinesePLA General HospitalBeijing 100039China School of Chemistry Biology and Material ScienceEast China University of TechnologyNanchang 330013China

Objective:Fufang Biejia Ruangan Tablet(FBRT) is widely used for the treatment of liver fibrosis.However,Hominis Placenta(HP),as an important adjuvant of FBRT,has been restricted for medicinal using due to the limited availability,ethical controversy and safety issues.The present study aimed to investigate the therapeutic effects of novel FBRT(N-FBRT) with sheep placenta(SP) as substitute for HP on liver fibrosis and explore its possible mechanisms.Different dosages of SP in N-FBRT were also evaluated.Methods:Rats were subcutaneously injected with CCl_(4)to induce liver fibrosis and then treated with NFBRT and FBRT.The anti-hepatic fibrosis effect was determined based on biomarkers analysis of liver function and hepatic fibrosis,and the liver pathology was visualized by H&E staining and Masson staining.The oxidative stress and inflammatory cytokines were also detected.Immunohistochemical staining of a-SMA,real time PCR and Western blotting were performed to evaluate hepatic stellate cells(HSCs)activation and TGF-β1/Smad signaling pathway.Results:N-FBRT and FBRT could ameliorate CCl_(4)-induced liver fibrosis and improve liver function,as evidenced by lowering serum biomarkers levels of liver function and hepatic fibrosis,and decreasing hepatic Hyp content and collagen deposition,and improving the hepatic morphology and architecture changes.Moreover,the anti-liver fibrosis effect was better when the dosage of SP used in N-FBRT was 1/2 of HP in FBRT.Administration of N-FBRT markedly alleviated oxidative stress and inflammatory cytokines,and inhibited a-SMA expression.Furthermore,the mRNA expression of Col Ⅰ,Col Ⅲ,a-SMA and TGF-β1,and proteins expression of a-SMA,TGF-β1,Smad2/3 and p-Smad2/3 were significantly down-regulated by N-FBRT treatment.Conclusion:SP can be used as substitute for HP to prepare N-FBRT for the treatment of liver fibrosis and the anti-liver fibrosis effect of N-FBRT is achieved by eliminating oxidative stress and inflammation,and inhibiting HSCs activation and ECM production by blocking TGF-β1/S

关键词： Fufang Biejia Ruangan Tablet Hominis Placenta liver fibrosis sheep placenta substitute TGF-β1/Smad signaling pathway

The contribution of absorption of integral nanocrystals to enhancement of oral bioavailability of quercetin

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第4期11卷 978-988页

作者： Baode shen chengying shen Weifeng Zhu Hailong Yuan Department of Pharmacy Air Force Medical CenterPLABeijing 100142China Key Lab of Modern Preparation of Traditional Chinese Medicine(TCM) Ministry of EducationJiangxi University of TCMNanchang 330004China

In this study,self-discriminating hybrid nanocrystals was utilized to explore the biological fate of quercetin hybrid nanocrystals(QT-HNCs)with diameter around 280 nm(QT-HNCs-280)and550 nm(QT-HNCs-550)following oral and intravenous administration and the contribution of integral nanocrystals to oral bioavailability enhancement of QT was estimated by comparing the absolute exposure of integral QT-HNCs and total QT in the liver.Results showed that QT-HNCs could reside in vivo as intact nanocrystals for as long as 48 h following oral and intravenous administration.A higher accumulation of integral QT-HNCs in liver and lung was observed for both oral and intravenous administration of QT-HNCs.The particle size affects the absorption and biodistribution of integral QT-HNCs and total QT.As compared to QT-HNCs-550,QT-HNCs-280 with smaller particle size is more easily absorbed,but dissolves faster in vivo,leading to higher distribution of QT(146.90 vs.117.91 h·mg/mL)but lower accumulation of integral nanocrystals(6.82 e10 vs.15.27 e10 h·[p/s]/[μW/cm2])in liver following oral administration.Due to its slower dissolution and enhanced recognition by RES,QT-HNCs-550 with larger diameter shows higher liver distribution for both of QT(1015.80 h·mg/mL)and integral nanocrystals(259.63 e10 h·[p/s]/[μW/cm2])than those of QT-HNCs-280(673.82&77.66 e10 h·[p/s]/[μW/cm2])following intravenous administration.The absolute exposure of integral QT-HNCs in liver following oral administration of QT-HNCs are 8.78%for QT-HNCs-280 and 5.88%for QT-HNCs-550,while the absolute exposure of total QT for QT-HNCs-280 and QT-HNCs-550 are 21.80%and 11.61%,respectively.Owing to imprecise quantifcation method,a surprisingly high contribution of integral QT-HNCs to oral bioavailability enhancement of QT(40.27%for QT-HNCs-280 and 50.65%for QT-HNCs-550)was obtained.These results revealed signifcant difference in absorption and biodistrbution between integral nanocrystals and overall drugs following oral and intravenous administration of QT-HNCs,and provided a

关键词： Nanocrystals Biological fate Quercetin Pharmacokinetics Biodistribution Oral bioavailability Contribution Drug delivery

In vivo dissolution of poorly water-soluble drugs:Proof of concept based on fluorescence bioimaging

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第4期11卷 1056-1068页

作者： Yinqian Yang Yongjiu Lv chengying shen Tingting Shi Haisheng He Jianping Qi Xiaochun Dong Weili Zhao Yi Lu Wei Wu Key Laboratory of Smart Drug Delivery of MOE School of PharmacyFudan UniversityShanghai 201203China Center for Medical Research and Innovation Shanghai Pudong HospitalFudan University Pudong Medical CenterShanghai 201399China

In vitro-in vivo correlation(IVIVC)of solid dosage forms should be established basically between in vitro and in vivo dissolution of active pharmaceutical ingredients.Nevertheless,in vivo dissolution profles have never been accurately portrayed.The current practice of IVIVC has to resort to in vivo absorption fractions(Fa).In this proof-of-concept study,in vivo dissolution of a model poorly watersoluble drug fenofbrate(FNB)was investigated by fuorescence bioimaging.FNB crystals were frst labeled by near-infrared fuorophores with aggregation-caused quenching properties.The dyes illuminated FNB crystals but quenched immediately and absolutely once been released into aqueous media,enabling accurate monitoring of residual drug crystals.The linearity established between fuorescence and crystal concentration justifed reliable quantifcation of FNB crystals.In vitro dissolution was frst measured following pharmacopoeia monograph protocols with well-documented IVIVC.The synchronicity between fuorescence and in vitro dissolution of FNB supported using fuorescence as a measure for determination of dissolution.In vitro dissolution correlated well with in vivo dissolution,acquired by either live or ex vivo imaging.The newly established IVIVC was further validated by correlating both in vitro and in vivo dissolution with Faobtained from pharmacokinetic data.

关键词： In vivo dissolution Fenofibrate Fluorescence Aggregation-caused quenching Bioimaging IVIVC

The Construction of the Major Clusters in Higher Vocational Colleges from the Perspective of Industrial Cluster

在线全文

学校读者我要写书评

暂无评论

International Journal of Technology Management 2015年第9期 14-16页

作者： Qingtang ZHANG chengying shen Jiangyin Polytechnic College Jiangyin 214405 Jiangsu China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于工程实践的污水处理教学平台设计

电子技术（上海） 2022年第3期51卷 230-231页

作者：徐娜沈澄英江阴职业技术学院江苏214405

阐述教学平台在污水处理工程实践中的应用,提出创新解决方法,包括理论与实践融合、工艺流程为主线,从而实现知识体系模块化、教学手段多样化、教学评价多面化。

关键词：创新教学工程实践知识体系教学平台

“三全育人”视域下高职大学生职业素养培养探赜

在线全文

学校读者我要写书评

暂无评论

成才之路 2023年第19期 5-8页

作者：李敏尚润玲沈澄英江阴职业技术学院江苏江阴214405

“三全育人”理念和陶行知提出的“家庭、学校、社会”三位一体教育理念相呼应,指通过全员育人、全过程育人、全方位育人清除传统高校思想道德教育存在的盲区,让思想教育、职业教育(技能教育、知识教育)、素养教育充分渗透到大学生的生... 详细信息

“三全育人”理念和陶行知提出的“家庭、学校、社会”三位一体教育理念相呼应,指通过全员育人、全过程育人、全方位育人清除传统高校思想道德教育存在的盲区,让思想教育、职业教育(技能教育、知识教育)、素养教育充分渗透到大学生的生活和学习中。文章在“三全育人”视角下对高职大学生职业素养培育展开论述,对“三全育人”的内涵、高职大学生职业素养组成、现状展开分析,并讨论职业素养培养的有效途径。

关键词：三全育人高职大学生职业素养课程思政环境育人五育并举

三全育人视域下高职纺织专业大学生职业素养培育探赜

在线全文

学校读者我要写书评

暂无评论

成才之路 2022年第30期 9-12页

作者：李敏尚润玲沈澄英江阴职业技术学院江苏江阴214405

文章从三全育人与职业素养概述、三全育人视域下高职纺织专业学生职业素养培育现状、三全育人视域下高职纺织专业学生职业素养培育策略三方面进行探讨,指出高职院校要加强思政教育与职业素养教育的结合,依托思政教育载体锻炼学生的职业... 详细信息

文章从三全育人与职业素养概述、三全育人视域下高职纺织专业学生职业素养培育现状、三全育人视域下高职纺织专业学生职业素养培育策略三方面进行探讨,指出高职院校要加强思政教育与职业素养教育的结合,依托思政教育载体锻炼学生的职业素养,加强教师队伍建设,紧扣全员育人构建职业素养培育模式,紧扣全程育人构建职业素养培育机制,紧扣全方位育人制定职业素养培育方案,强化校本实施并对教学方法进行创新,拓展职业素养选修课程,深挖教育渠道并加强职业素养在专业课程中的融入。

关键词：高职院校三全育人纺织专业职业素养