检索结果-南通市图书馆

Characterization of miRNomes in Acute and Chronic Myeloid Leukemia Cell Lines

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2014年第2期12卷 79-91页

作者： Qian Xiong Yadong Yang Hai wang Jie Li shaobin wang Yanming Li Yaran Yang Kan Cai Xiuyan Ruan Jiangwei Yan Songnian Hu Xiangdong Fang CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences University of Chinese Academy of Sciences

Myeloid leukemias are highly diverse diseases and have been shown to be associated with microRNA（miRNA） expression aberrations. The present study involved an in-depth miRNome analysis of two human acute myeloid leukemia（AML） cell lines, HL-60 and THP-1, and one human chronic myeloid leukemia（CML） cell line, K562, via massively parallel signature sequencing. mRNA expression profiles of these cell lines that were established previously in our lab facilitated an integrative analysis of miRNA and mRNA expression patterns. miRNA expression profiling followed by differential expression analysis and target prediction suggested numerous miRNA signatures in AML and CML cell lines. Some miRNAs may act as either tumor suppressors or oncomiRs in AML and CML by targeting key genes in AML and CML pathways. Expression patterns of cell type-specific miRNAs could partially reflect the characteristics of K562, HL-60 and THP-1 cell lines, such as actin filament-based processes, responsiveness to stimulus and phagocytic activity. miRNAs may also regulate myeloid differentiation, since they usually suppress differentiation regulators. Our study provides a resource to further investigate the employment of miRNAs in human leukemia subtyping, leukemogenesis and myeloid development. In addition, the distinctive miRNA signatures may be potential candidates for the clinical diagnosis, prognosis and treatment of myeloid leukemias.

关键词： miRNA profiling Acute myeloid leukemia Chronic myeloid leukemia Myeloid differentiation

Liver injury after intermittent or continuous hepatic pedicle clamping and its protection by reduced glutathione

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 209-213页

作者： Li Zhou JingAn Rui RouLi Zhou XueMin Peng shaobin wang ShuGuang Chen Qiang Qu YuPei Zhao Department of General Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100032 China. lizhou02@***

BACKGROUND:The debate is still going on about selection of several clamping patterns during hepatectomy. The aim of this study was to assess the safety and preference of normothermic intermittent or continuous hepatic pedicle clamping and confirm the protective effect of reduced glutathione (GSH).;METHODS:Thirty-two adult male healthy Sprague-Dawley (SD) rats were divided into groups of intermittent clamping and GSH absent (IA), continuous clamping and GSH absent (CA), intermittent clamping and GSH present (IP) and continuous clamping and GSH present (CP). The clamping manners were successively 40 minutes in continuous clamping groups and two cycles of 20 minutes with an interval of 5 minutes in intermittent clamping groups, and reperfusion periods were 60 minutes. Experimental parameters included levels of malonaldehyde (MDA) and Cu/Zn superoxide dismutase (SOD), pathological and ultrastructural changes in liver tissues, activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in sera.;RESULTS:In the same group, the activities of ALT and AST were significantly higher in post-clamping rats than in pre-clamping rats (P0.05). The differences of all values between post-reperfusion rats and pre-clamping rats were significant (P0.05). The values were significantly different between the GSH absent and present groups after reperfusion (P<0.05). The morphological damages were also obviously alleviated in the GSH present group.;CONCLUSIONS:Normothermic intermittent or continuous hepatic pedicle clamping could cause reversible liver ischemia/reperfusion injury when the clamping time lasts 40 minutes. The in

关键词： hepatic pedicle clamping hepatic ischemia/reperfusion injury reduced glutathione protection

The Inhibitory Effect of Oridonin on the Growth of Fifteen Human Cancer Cell Lines

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Clinical Oncology 2007年第1期4卷 16-20页

作者： Junhui Chen shaobin wang Dongyang Chen Guisheng Chang Qingfeng Xin Shoujun Yuan Zhongying Shen Department of Oncology The First Affiliated Hospital of Shantou University Medical College Shantou 515041 China Pharmacology Laboratory Institute of Radiation Medicine Military Medical Sciences Academy of Chinese PLA Beijing 100270 China Department of Pathology Shantou University Medical College Shantou 515031 China

OBJECTIVE To study the inhibitory effect of oridonin on the growth of cancer cells. METHODS Fifteen human cancer cell lines were subjected to various concentrations of oridonin in culture medium. The inhibitory rate of cell growth was measured by the MTT assay, and compared with a negative control and 5-Fu-positive control. RESULTS The 50% inhibiting concentration （IC50） and maximal inhibition （Imax） of oridonin shown by studying the growth of the cancer cell lines were as follows： leukemias （HL60 cells： 3.9 μg/ml and 73.8%, K562 cells： 4.3 μg/ml and 76.2%）; esophageal cancers（SHEEC cells： 15.4 μg/ml and 99.2%, Eca109 cells： 15.1 μg/ml and 84.6%, TEl cells： 4.0 μg/ml and 70.2%）; gastric cancers （BGC823 cells： 7.6 μg/ml and 98.7%, SGC7901 cells： 12.3 μg/ml and 85.7%）; colon cancers （HT29 cells： 13.6 μg/ml and 97.2%, HCT cells： 14.5 μg/ml and 96.5%）; liver cancers （Bel7402 cells： 15.2 μg/ml and 89.2%, HepG2 cells： 7.1 μg/ml and 88.3%）; pancreatic cancer （PC3 cells： 11.3 μg/ml and 68.4%）; lung cancer （A549 cells： 18.6 μg/ml and 98.0% ）; breast cancer （MCF7 cells： 18.4 μg/ml and 84.7%）; uterine cervix cancer （Hela cells： 13.7 μg/ml and 98.5%）. CONCLUSION Oridonin had a relatively wide anti-tumor spectrum, and a relatively strong inhibitory effect on the growth of the 15 human cancer cells. Inhibitory effects were concentration dependent.

关键词： oridonin antitumor effect in vitro study.

Progress in the development of techniques based on light scattering for single nanoparticle detection

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2011年第8期54卷 1244-1253页

作者： ZHU shaobin, wang Shuo, YANG LingLing, HUANG TianXun & YAN XiaoMei Department of Chemical Biology, College of Chemistry and Chemical Engineering Key Laboratory for Chemical Biology of Fujian Province Key Laboratory of Analytical Science, Xiamen University, Xiamen 361005, China Department of Chemical Biology College of Chemistry and Chemical Engineering Key Laboratory for Chemical Biology of Fujian Province Key Laboratory of Analytical Science Xiamen University Xiamen China

Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparficles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles.

关键词： light scattering single particle detection nanoparticles confocal correlation spectroscopy flow cytometry

在线全文

学校读者我要写书评

暂无评论

文山州青贮玉米育种研究、生产现状及对策

草学 2024年第4期 83-86页

作者：钱双宏朱汉勇王绍斌伍丽仙罗文娇唐丽敏蔡世昆文山壮族苗族自治州农业科学院云南文山663099 文山州畜牧站云南文山663099

青贮玉米是草食家畜优质高产饲料的重要来源之一,发展青贮玉米产业是玉米产业结构调整和推进国家“粮改饲”项目的重要一环。本文对文山州青贮玉米的生产和育种研究现状、存在的问题进行深入剖析,提出发展青贮玉米的几点建议及育种对策... 详细信息

青贮玉米是草食家畜优质高产饲料的重要来源之一,发展青贮玉米产业是玉米产业结构调整和推进国家“粮改饲”项目的重要一环。本文对文山州青贮玉米的生产和育种研究现状、存在的问题进行深入剖析,提出发展青贮玉米的几点建议及育种对策,为文山州玉米产业结构调整和畜牧业健康可持续发展提供参考,同时将为全州巩固拓展脱贫攻坚成果同乡村振兴有效衔接提供高质量的产业支撑。

关键词：青贮玉米育种生产现状发展对策文山

维普期刊数据库博看期刊

在线全文

学校读者我要写书评

暂无评论

改进PCA-SIFT算法的立体匹配系统

激光与光电子学进展 2016年第3期53卷 171-177页

作者：于之靖王韶彬中国民航大学航空地面特种设备研究基地天津300300 中国民航大学航空自动化学院天津300300

针对双目视觉测量中的立体匹配问题,提出了一种基于主成分分析(PCA)算法和尺度不变特征变换(SIFT)匹配算法相结合的双目视觉立体匹配新方法。该系统以GSI编码点为特征点,利用双目相机对以散斑为背景的编码标识板进行成像,利用PCA算法与S... 详细信息

针对双目视觉测量中的立体匹配问题,提出了一种基于主成分分析(PCA)算法和尺度不变特征变换(SIFT)匹配算法相结合的双目视觉立体匹配新方法。该系统以GSI编码点为特征点,利用双目相机对以散斑为背景的编码标识板进行成像,利用PCA算法与SIFT算法结合的新算法对采集的图像进行特征提取与匹配解算。可实现对GSI编码点特征的准确提取和立体匹配,并测量出不同位姿下特征点之间的精确位移。实验验证部分引入GSI编码技术并在行程为1000 mm×1000 mm二维高精度平移台上进行,实际测得位移的绝对误差在5×10-2mm之内,验证了该系统的高精确性。

关键词：测量双目视觉系统立体匹配主成分分析-尺度不变特征变换算法 GSI编码点高精确性

在线全文

学校读者我要写书评

暂无评论

商贸流通业对陕西省社会经济发展贡献分析

商业时代 2011年第5期 25-26页

作者：王少斌西京学院西安710123

伴随着产业结构高度化,第三产业在国民经济中的比重不断上升,对经济的促进作用不断增强,而商贸流通业是第三产业的重要组成部分,近年来得到了广泛深入的发展。为直观表述商贸流通业对陕西经济发展的贡献,本文对1978～2009年陕西GDP和社... 详细信息

伴随着产业结构高度化,第三产业在国民经济中的比重不断上升,对经济的促进作用不断增强,而商贸流通业是第三产业的重要组成部分,近年来得到了广泛深入的发展。为直观表述商贸流通业对陕西经济发展的贡献,本文对1978～2009年陕西GDP和社会消费品零售总额的数据进行检验,研究了商贸流通业对陕西省社会经济的影响。结果表明,商贸流通业对于促进陕西经济发展确实发挥着积极的作用。

关键词：商贸流通业经济发展贡献

Single‐atom catalysts on metal‐based supports for solar photoreduction catalysis

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Catalysis 2022年第9期43卷 2301-2315页

作者： Huayang Zhang Wenjie Tian Xiaoguang Duan Hongqi Sun Yingping Huang Yanfen Fang shaobin wang School of Chemical Engineering and Advanced Materials The University of AdelaideAdelaideSA 5005Australia School of Engineering Edith Cowan University270 Joondalup DriveJoondalupWA 6027Australia Engineering Research Center of Eco‐environment in Three Gorges Reservoir Region Ministry of EducationChina Three Gorges UniversityYichang 443002HubeiChina

Metal atoms atomically dispersed on an inorganic metal‐based support compose a unique category of single atom catalysts(SACs)and have important applications in catalytic photoreduction reactions,including H_(2) evolution reaction,CO_(2) reduction reaction,and N_(2) reduction *** this minreview,we summarized the typical metal‐support interaction(M‐SI)patterns for successful anchoring of single‐atom metals on metallic compound ***,the contribution of the dispersed single metal atoms and M‐SI to photocatalytic reactions with improved activity,selectivity,and stability are highlighted,such as by accelerating charge transfer,regulating band structure of the support,acting as the reductive sites,and/or increasing catalytic ***,some challenges and perspectives of future development are *** anticipate that this minireview will be a beneficial supplement for a comprehensive perception of metal‐based material supported SACs and their application in heterogeneous photo‐reductive catalysis.

关键词： Single atom catalyst Metal‐based support Metal‐support interaction Photocatalysis Solar energy conversion

在线全文

学校读者我要写书评

暂无评论

柏树底铁路隧道地下采空区稳定性分析

铁道建筑 2005年第12期45卷 30-32页

作者：王少斌铁道第二勘察设计院西安分院西安710054

以采空区上覆岩层的沉陷特征为理论依据,利用有限的地质资料,对柏树底隧道采空区的稳定性进行分析评价,预测采空区对隧道的影响程度。

关键词：地下采空区沉陷特征稳定性分析