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Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus

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Zoological Research 2024年第2期45卷 381-397页

作者： Xi-Dan Xu Yue Zhou Chong-Qing Wang Xu Huang Kun Zhang Xiao-Wei Xu Li-Wen He Xin-Yue Zhang Xin-Zhu Fu Ming Ma Qin-Bo Qin shao-jun liu State Key Laboratory of Developmental Biology of Freshwater Fish Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education MinistryCollege of Life SciencesHunan Normal UniversityChangshaHunan 410081China Nansha-South China Agricultural University Fishery Research Institute GuangzhouGuangdong 511458China Hunan Yuelu Mountain Science and Technology Co.Ltd.for Aquatic Breeding ChangshaHunan 410081China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangshaHunan 410081China

The autotetraploid Carassius auratus(4nRR,4n=200,RRRR)is derived from whole-genome duplication of Carassius auratus red var.(RCC,2n=100,RR).In the current study,we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin(red in RCC,brownish-yellow in4nRR).To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR,we performed transcriptome profiling and molecular functional verification in RCC and *** revealed that scarb1,associated with carotenoid metabolism,underwent significant down-regulation in *** editing of this candidate pigment gene provided clear evidence of its significant role in RCC ***,we identified four divergent scarb1 homeologs in 4nRR:two original scarb1 homeologs from RCC and two duplicated ***,three of these homeologs possessed two highly conserved alleles,exhibiting biased and allelespecific expression in the ***,after precise editing of both the original and duplicated scarb1homeologs and/or alleles,4nRR individuals,whether singly or multiply mutated,displayed a transition from brownishyellow skin to a cyan-gray ***,the proportional areas of the cyan-gray regions displayed a gene-dose *** findings illustrate the subfunctionalization of duplicated scarb1,with all scarb1genes synergistically and equally contributing to the pigmentation of *** is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploidfish,substantiallyenrichingour understanding of coloration formation and change within this group of organisms.

关键词： Autopolyploidization Coloration change scarb1 Functional differentiation Genetic changes

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Microinjection of a growth factor cocktail affects activated microglia in the neocortex of adult rats

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Neural Regeneration Research 2020年第9期15卷 1709-1715页

作者： Ruo-Xu liu Jie Ma Ning Guo shao-jun liu State Key Laboratory of Proteomics and Department of Neurobiology Institute of Military Cognition and Brain Sciences

Microglia, as the resident immune cells in the central nervous system, play important roles in regulating neuronal processes, such as neural excitability, synaptic activity, and apoptotic cell clearance. Growth factors can activate multiple signaling pathways in central nervous system microglia and can regulate their immune effects, but whether growth factors can affect the morphological characteristics and ultrastructure of microglia has not been reported. After microinjecting 300 nL of a growth factor cocktail, including 10 μg/mL epidermal growth factor, 10 μg/mL basic fibroblast growth factor, 10 μg/mL hepatocyte growth factor and 10 μg/mL insulin-like growth factor into adult rat cortex, we found that the number of IBA1-positive microglia around the injection area increased significantly, indicating local activation of microglia. All CD68-positive labeling co-localized with IBA1 in microglia. Cell bodies and protrusions of CD68-positive cells were strongly attached to or were engulfing neurons. Characteristic huge phagosomes were observed in activated phagocytes by electron microscopy. The phagosomes generally included non-degraded neuronal protrusions and mitochondria, yet they contained no myelin membrane or remnants, which might indicate selective phagocytosis by the phagocytes. The remnant myelin sheath after phagocytosis still had regenerative ability and formed "myelin-like" structures around phagocytes. These results show that microinjection of a growth factor cocktail into the cerebral cortex of rodents can locally activate microglia and induce selective phagocytosis of neural structures by phagocytes. The study was approved by the Institute of Laboratory Animal Science, Beijing Institute of Basic Medical Sciences(approval No. IACUC-AMMS-2014-501) on june 30, 2014.

关键词： adult neocortex CD68 IBA1 microinjection phagocyte selective phagocytosis ultrastructure

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No DCX-positive neurogenesis in the cerebral cortex of the adult primate

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Neural Regeneration Research 2020年第7期15卷 1290-1299页

作者： Ruo-Xu liu Jie Ma Bin Wang Tian Tian Ning Guo shao-jun liu Institute of Military Cognition and Brain Sciences BeijingChina Department of Pharmacy Medical CollegeHuanghe S&T UniversityZhengzhouHenan ProvinceChina

Whether endogenous neurogenesis occurs in the adult cortex remains *** increasing number of reports suggest that doublecortin(DCX)-positive neurogenesis persists in the adult primate cortex,attracting enormous attention *** this study,different DCX antibodies were used together with NeuN antibodies in immunohistochemistry and western blot assays using adjacent cortical sections from adult *** adsorption,antigen binding,primary antibody omission and antibody-free experiments were used to assess specificity of the *** found either strong fluorescent signals,medium-weak intensity signals in some cells,weak signals in a few perikarya or near complete lack of labeling in adjacent cortical sections incubated with the various DCX *** putative DCX-positive cells in the cortex were also positive for NeuN,a specific marker of mature ***,further experiments showed that most of these signals were either the result of antibody cross reactivity,the non-specificity of secondary antibodies or tissue *** confirmed DCX-positive cells were detected in the adult macaque cortex by *** findings show that DCX-positive neurogenesis does not occur in the cerebral cortex of adult primates,and that false-positive signals(artefacts)are caused by antibody cross reactivity and *** experimental protocols were approved by the Institutional Animal Care and Use Committee of the Institute of Neuroscience,Beijing,China(approval ***-AMMS-2014-501).

关键词： adult neurogenesis antigen neutralization autofluorescence cross reaction cross reactivity doublecortin neocortex NeuN non-specificity primates tissue adsorption

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Role of HCN channels in the nervous system:membrane excitability and various modulations

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中国应用生理学杂志 2014年第6期30卷 506-510页

作者： Xie-chuan WENG shao-jun liu Department of Neurobiology and State Key Laboratory of Proteoniics Beijing Institute of Basic Medical Sciences

Hyperpolarization-activated and cyclic nucleotide-gated(HCN) channels,distributing in a variety of tissues,especially in excitable cells such as heart cells and many kinds of neurons,have an important role in the modulation of heart rate and neuronal *** from typical voltage-gated sodium channels and potassium channels,HCN channels were evoked inward currents when the cell was *** and more recent studies have disclosed that HCN channels play important roles in the nervous system,which were linked with its special electrophysiological features as well as its regulatory effect on the cellular membrane *** channels could be modulated by many factors including both extracellular molecules and intracellular signaling cascades,which made its functions complicated in the different *** on its role,HCN channels are presumed to be a promising target for chronic pain and brain *** this paper,we will focus on the advancement of roles of HCN channels in the neural system as well as its complex modulator factors.

关键词：调制因子神经系统 N通道 HCN 兴奋性细胞膜电压门控钠通道可兴奋细胞

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Central projections and connections of lumbar primary afferent fibers in adult rats:effectively revealed using Texas red-dextran amine tracing

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Neural Regeneration Research 2017年第10期12卷 1695-1702页

作者： Shi-de Lin Tao Tang Ting-bao Zhao shao-jun liu State Key Laboratory of Proteomics Department of NeurobiologyBeijing Institute of Basic Medical Sciences Department of Spinal Cord Injury the General Hospital of Jinan Military Command

Signals from lumbar primary afferent fibers are important for modulating locomotion of the ***,silver impregnation techniques,autoradiography,wheat germ agglutinin-horseradish peroxidase and cholera toxin B subunit-horseradish peroxidase cannot image the central projections and connections of the dorsal root in ***,we injected 3-k Da Texas red-dextran amine into the proximal trunks of L4 dorsal roots in adult *** microscopy results revealed that numerous labeled arborizations and varicosities extended to the dorsal horn from T12–S4,to Clarke＇s column from T10–L2,and to the ventral horn from L1–*** labeled varicosities at the L4 cord level were very dense,particularly in laminae I–Ⅲ,and the density decreased gradually in more rostral and caudal *** addition,they were predominately distributed in laminae I–IV,moderately in laminae V–VⅡ and sparsely in laminae VⅢ–***,direct contacts of lumbar afferent fibers with propriospinal neurons were widespread in gray *** conclusion,the projection and connection patterns of L4 afferents were illustrated in detail by Texas red-dextran amine-dorsal root tracing.

关键词： nerve regeneration spinal cord injury dorsal root central projection connection Texas red-dextran amine neural regeneration

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A Gain-of-Function Mutation in OslAA11 Affects Lateral Root Development in Rice

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Molecular Plant 2012年第1期5卷 154-161页

作者： Zhen-Xing Zhu Yu liu shao-jun liu Chuan-Zao Mao Yun-Rong Wu Ping Wu State Key Laboratory of Plant Physiology and Biochemistry Zhejiang UniversityHangzhou 310058People's Republic of China College of Life Sciences Zi Jin Gang CampusZhejiang University866 Yu Hang Tang RoadHangzhou310058People's Republic of China

Lateral roots are important to plants for the uptake of nutrients and water. Several members of the Aux/IAA family have been shown to play crucial roles in lateral root development. Here, a member of the rice Aux/IAA family genes, OslAA 11 （LOC_Os03g43400）, was isolated from a rice mutant defective in lateral root development. The gain-of-function mutation in OslAAll strictly blocks the initiation of lateral root primordia, but it does not affect crown root development. The expression of OslAAll is defined in root tips, lateral root caps, steles, and lateral root primordia. The auxin reporter DR5-GUS （^-glucuronidase） was expressed at lower levels in the mutant than in wild-type, indicating that OslAAll is involved in auxin signaling in root caps. The transcript abundance of both OsPINlb and OsPIN10a was diminished in root tips of the Osiaa 11 mutant. Taken together, the results indicate that the gain-of-function mutation in OslAA 11 caused the inhibition of lateral root development in rice.

关键词： Oryza sativa L. OslAAll lateral root auxin signaling.

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A rare case of spontaneous renal cholesterol crystallization embolism

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Chinese Medical Journal 2019年第3期132卷 367-369页

作者： Ling-Yun Chen Yan-Ping Huang shao-jun liu Pei-Jyu Mao Department of Nephrology Tongren HospitalShanghai Jiao Tong University School of MedicineShanghai 200336China Department of Nephrology Huashan HospitalFudan UniversityShanghai 200040China Department of Nephrology Shanghai Electric Power Hospital

To the Editor: Cholesterol crystal embolism (CCE) is a systemic disease caused by cholesterol crystal embolization leading to the occlusion of small arteries in a variety of organs. It may occur spontaneously, or more often, after intravascular procedures such as coronary angiography and carotid artery stenting, cardiovascular surgery such as coronary artery bypass grafting or aortic aneurysm surgery.[1] The kidneys were previously reported to be the most frequent target organ for CCE, and renal CCE was found during the course of clinical illness in approximately 50% of patients with CCE.

关键词： Cholesterol crystal embolism (CCE) small arteries coronary angiography

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Deposition of thin TiO_(2)films on fluorophlogopite particle surface by CVD in fluidized bed reactor to prepare silver pearlescent pigments

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Particuology 2024年第6期89卷 88-98页

作者： Ming liu Guoqiang shao Pengpeng Lv jun Li Qingshan Zhu State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China

Two critical parameters:Glossiness and chromaticity space of pearlescent pigments were evaluated based on crystallinity and grain *** the roughness and the vertical distance evolution along the surface of deposited TiO_(2)film are affected by the *** optimal crystallinity of TiO_(2)was deposited at the temperature of 350℃.The TiCl4 concentration effectively changed the grain ***,the high flow rate of fluidizing gas and optimal gas–solid ratio(<0.11 m^(3)/(kg h))restricted the change of TiCl_(4)concentrations in fluidized bed *** this case,the grain size of the deposited TiO_(2)film was about 55 *** on the grain size,the color of pearlescent pigments was adjusted by controlling the film thickness based on TiO_(2)***,the silver pearlescent pigment with high glossiness was prepared by chemical vapor deposition method in a fluidized bed reactor.

关键词： Fluidized bed chemical vapor deposition TiO_(2)film deposition Pearlescent pigment Free powder restrain Crystal growth

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To be or not to be direct:The role of neuromedin U in neuro-eosinophil crosstalk

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Science Bulletin 2024年第2期69卷 148-150页

作者： Ruichao liu Wenhao shao jun Xu Coco Chu SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine Taiyuan 030001China Insttute for Immunogy Department of Basic Medical SciencesSchool of Medicinesinghua UnierstyBejing 100084China Tsinghua-Peking Center for Life Sciences Tsinghua UniversityBeijing 100084China Shanxi Medical University Taiyuan 030012China Department of Surgery First Hospital of Shanxi Medical UniversityShanxi Provincial Key Laboratory for Digestive Diseases and Organ TransplantationTaiyuan 030001China Beijing Key Laboratory for Immunological Research on Chronic Diseases Tsinghua UniversityBejing 100084China

With the development of new experimental techniques(e.g.,single-cell RNA sequencing(scRNA-seq),light sheet microscopy,and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9),emerging studies have begun to uncover the interactions between the nervous system and the immune system,both in health and *** example,in mucosal tissues such as the lung and the intestine,neurotransmitters including acetylcholine[1]and adrenaline[2]can directly regulate the immune cell functions thus affect the results of allergy,asthma,and anti-pathogen host defense[3].

关键词： intestine lung asthma

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Renal Phospholipase A2 Receptor and the Clinical Features of Idiopathic Membranous Nephropathy

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Chinese Medical Journal 2017年第8期130卷 892-898页

作者： Ning-Xin Xu Qiong-Hong Xie Zhu-Xing Sun Jia Wang Yan Li Liang Wang shao-jun liu jun Xue Chuan-Ming Hao Department of Nephrology Huashan Hospital Fudan University Shanghai 200040 China Department of Nephrology Wuxi People's Hospital Wuxi Jiangsu 214023 China

Background： According to the renal phospholipase A2 receptor （PLA2R） immunohistochemistry, idiopathic membranous nephropathy （iMN） could be categorized into PLA2R-associated and non-PLA2R-associated iMN. This study aimed to examine whether the non-PLA2R-associated iMN had any difference in clinical features compared with PLA2R-associated iMN. Methods： A total of 231 adult patients diagnosed as iMN were recruited to this retrospective study. Renal PLA2R expression was examined by immunofluorescence. Among these patients, 186 （80.5%） with complete baseline clinical data were used for further study. Urinary protein excretion, serum albumin, and creatinine were analyzed. For those patients with follow-up longer than 1 year, the relationship between PLA2R and response to immunosuppressants were analyzed. The t-test was used for parametric analysis and the Mann-Whitney U-test was used for nonparametric analysis. Categorical variables were described as frequencies or percentages, and the data were analyzed with Pearson＇s Chi-squaxe test or Fisher＇s exact test. Results： Of the 231 iMN patients, 189 showed renal detectable PLA2R expression （81.8%）. The baseline serum creatinine, serum albumin, and urine protein excretion were not significantly different between PLA2R-associated （n = 145） and non-PLA2R-associated iMN patients （n = 41）. However, about 1/3 of the non-PLA2R-associated iMN had abnormal serological tests, significantly more common than PLA2R-associated iMN （31.7% vs. 8.3%, P = 0.000）. The non-PLA2R-associated iMN had lower C4 levels compared with PLA2R-associated iMN （P = 0.004）. The non-PLA2R-associated iMN patients also showed a better response to immunosuppressants （complete remission [CR] 42.9%; partial remission [PR] 14.3%） compared with PLA2R-associated iMN （CR 3.2%; PR 48.4%, P = 0.004） at the 3rd month. Conclusions： There were no significant differences in serum creatinine, albumin, and urine protein excretion between PLA2R-associated and non-PLA2R-associa

关键词： lmmunosuppressive Therapy Membranous Nephropathy Phospholipase A2 Receptor Serology

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