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Evaluation of the early defoliation trait and identification of resistance genes through a comprehensive transcriptome analysis in pears

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Journal of Integrative Agriculture 2023年第1期22卷 120-138页

作者： shan Yan-fei LI Meng-yan wang Run-ze LI Xiao-gang LIN Jing LI Jia-ming ZHAO Ke-jiao WU Jun College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095P.R.China Institute of Horticulture Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing 210095P.R.China

Early defoliation,which usually occurs during summer in pear trees,is gradually becoming a major problem that poses a serious threat to the pear industry in southern ***,there is no system for evaluating the responses of different cultivars to early defoliation,and our knowledge of the potential molecular regulation of the genes underlying this phenomenon is still *** this study,we conducted field investigations of 155 pear accessions to assess their resistance or susceptibility to early defoliation.A total of 126 accessions were found to be susceptible to early defoliation,and only 29 accessions were *** them,19 resistant accessions belong to the sand pear species(Pyrus pyrifolia).To identify the resistance genes related to early defoliation,the healthy and diseased samples of two sand pear accessions,namely,the resistant early defoliation accession‘Whasan’and the susceptible early defoliation accession‘Cuiguan’,were used to perform RNA *** with‘Cuiguan’,a total of 444 genes were uniquely differentially expressed in‘Whasan’.Combined with GO and KEGG enrichment analyses,we found that early defoliation was closely related to the stress ***,a weighted gene co-expression network analysis revealed a high correlation of WRKY and ethylene responsive factor(ERF)transcription factors with early defoliation *** study provides useful resistant germplasm resources and new insights into potentially essential genes that respond to early defoliation in pears,which may facilitate a better understanding of the resistance mechanism and molecular breeding of resistant pear cultivars.

关键词： pear early defoliation resistance and susceptibility evaluation RNA-seq WRKY ERF

微光夜视镜对深径觉的影响及夜视深径觉体验训练的作用

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军事医学 2024年第3期48卷 161-165页

作者：于飞苏芳陈珊鲁婷婷张慧变田大为姚钦空军军医大学空军特色医学中心北京100142

目的研究微光夜视镜对深径觉的影响及夜视深径觉体验在改善深径觉中的作用。方法在暗室内,应用微光夜视镜、深径觉体验和测量控制装置等设备,对113名健康男性志愿者的暗环境裸眼深径觉及佩戴夜视镜后的深径觉进行体验及测量,重复3次,... 详细信息

目的研究微光夜视镜对深径觉的影响及夜视深径觉体验在改善深径觉中的作用。方法在暗室内,应用微光夜视镜、深径觉体验和测量控制装置等设备,对113名健康男性志愿者的暗环境裸眼深径觉及佩戴夜视镜后的深径觉进行体验及测量,重复3次,进行差异性分析,确定佩戴夜视镜对深径觉的影响,并通过方差分析,确定不同测量次数对深径觉测量结果的影响,明确深径觉测量与训练装置在改善深径觉中的作用。结果暗室内,受试者裸眼深径觉3次试验结果分别为(62.55±64.70)mm、(39.83±42.55)mm和(39.50±42.89)mm;佩戴夜视镜深径觉3次试验均值分别为(98.29±83.74)mm、(63.22±60.29)mm和(65.50±69.69)mm;与裸眼深径觉相比,佩戴夜视镜深径觉明显变差(P<0.05)。与第1次测量结果相比,不论是裸眼还是戴镜后深径觉第2、3次测量结果均明显改善,差异具有统计学意义(P<0.05)。结论佩戴夜视镜时,人眼深径觉会明显变差,但经过多次体验后,由于经验累积,深径觉能力会大幅增强。在戴镜执行夜间飞行任务前,有必要对飞行员进行戴镜深径觉测量与体验。

关键词：深径觉微光夜视镜夜视深径觉体验

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五味子内生菌及其代谢产物研究进展

包头医学院学报 2024年第4期40卷 91-96页

作者：王菲何珊于敬达高芳董彦宏戎松浩马淑一内蒙古科技大学包头医学院内蒙古包头014040

五味子是我国具有重要药用价值和经济价值的一类药用植物。而内生真菌、内生细菌和内生放线菌三大类是五味子内生菌的主要组成部分。五味子内生菌能产生与植株相同或相似的活性代谢产物,如木脂素、三萜、有机酸等活性物质。并且随着科... 详细信息

五味子是我国具有重要药用价值和经济价值的一类药用植物。而内生真菌、内生细菌和内生放线菌三大类是五味子内生菌的主要组成部分。五味子内生菌能产生与植株相同或相似的活性代谢产物,如木脂素、三萜、有机酸等活性物质。并且随着科学探索的持续进步,五味子内生菌在抗菌、抗癌、抗氧化以及病原菌生物防治等方面的作用也逐步被发掘出来,现已成为工业、医药、农业等相关领域的焦点所在。本文就近年来国内外有关五味子内生菌及次生代谢产物生物活性的研究进行综述,为五味子内生菌次生代谢产物的开发和应用提供理论支撑。

关键词：五味子内生菌次生代谢产物生物活性

Mapping the emergence of visual consciousness in the human brain via brain-wide intracranial electrophysiology

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The Innovation 2022年第3期3卷 88-96页

作者： Liang shan Hui Huang Zhiting Zhang Yuyin wang fei Gu Mingwei Lu Wen Zhou Yi Jiang Ji Dai Guangdong Provincial Key Laboratory of Brain Connectome and Behavior CAS Key Laboratory of Brain Connectome and ManipulationThe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions Shenzhen 518055China Department of Neurosurgery The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of PsychologyChinese Academy of SciencesBeijing 100101China

Consciousness lies at the heart of our existence and *** probe how perceptual consciousness emerges in the brain,we recorded brainwide intracranial electroencephalography signals from human patients while their perceptual consciousness was effectively manipulated using the continuous flash suppression *** observed substantial differences in brain activities when visual information gradually enters consciousness.

关键词： visual flash Mapping

医学节肢动物野外现场与实验室生物安全规范专家共识

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中国热带医学 2024年第2期24卷 119-125页

作者：何昌华罗欢乐尹飞飞韩谦梁磊师永霞于学东孙毅刘起勇王环宇王荣单超邓菲袁志明夏菡海南省疾病预防控制中心海南海口570203 海南省预防医学科学院海南海口570203 中山大学公共卫生学院(深圳) 广东深圳510006 海南医学院海南海口571199 海南大学生命健康学院海南海口570228 中国建筑科学研究院有限公司北京100013 广州海关技术中心广东广州510423 广州实验室广东广州510005 军事科学院军事医学研究院北京100850 中国疾病预防控制中心传染病预防控制所北京102206 中国疾病预防控制中心病毒病预防控制所北京102206 中国合格评定国家认可中心北京100062 中国科学院武汉病毒研究所湖北武汉430071

新发再发虫媒传染病不断涌现,给全球公共卫生安全带来严重威胁。医学节肢动物相关的野外现场和实验室研究对虫媒传染病的预防和控制至关重要。国内一直有不同机构或团队在开展相关研究工作,但是截至2023年,我国仍缺少对医学节肢动物相... 详细信息

新发再发虫媒传染病不断涌现,给全球公共卫生安全带来严重威胁。医学节肢动物相关的野外现场和实验室研究对虫媒传染病的预防和控制至关重要。国内一直有不同机构或团队在开展相关研究工作,但是截至2023年,我国仍缺少对医学节肢动物相关工作具有指导意义的生物安全规范或操作指南细则。为积极应对医学节肢动物相关活动中可能涉及到的生物安全问题,提高节肢动物生物安全相关操作、防护规范化和分级标准化水平,保障从业人员安全,制定本医学节肢动物野外现场和实验室生物安全规范专家共识,旨在指导我国医学节肢动物相关工作,保障国家生物安全。

关键词：医学节肢动物野外现场生物安全实验室生物安全专家共识

Value of Preoperative Hematological Parameters in the Prognosis of Gastric Cancer Patients Undergoing a Total Gastrectomy

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Current Medical Science 2022年第2期42卷 348-356页

作者： Xi-shan ZHU Ye ZHAO fei-yan MA Shi-kai WU Oncology Department Peking University First HospitalBeijing100034China Pathology Department Peking University First HospitalBeijing100034China

Objective This study aimed to evaluate the relationships between the albumin/globulin ratio(AGR),neutrophil/lymphocyte ratio(NLR),and platelet/lymphocyte ratio(PLR)and clinicopathological information for gastric cancer *** addition,the prognostic values of these hematological parameters for resectable gastric cancer patients undergoing a total gastrectomy were *** A total of 245 patients with gastric cancer who underwent a total gastrectomy at our hospital between January 1,2005,and December 30,2015,were enrolled into this *** preoperative AGR,NLR,and PLR in the serum samples of the patients were *** relationships between the hematological parameters and the disease-free survival(DFS)as well as overall survival(OS)were analyzed by statistical *** The cutoff values of AGR,NLR,and PLR were 1.57,3.5,and 193,*** analyses demonstrated that a low AGR,a high NLR,and a high PLR were significant risk factors for a poor *** to multivariate analysis,a high PLR was found to be independently associated with a poor ***,when age was considered as a stratified factor,univariate analyses demonstrated that a low AGR had the tendency to be correlated with a shorter DFS in nonelderly patients(<65 years old).A low AGR was significantly correlated with a shorter DFS and OS in elderly patients(≥65 years old).Conclusion AGR,NLR,and PLR are independent risk factors associated with a poor gastric cancer survival by univariate analysis,and AGR is an independent risk factor for predicting DFS and OS in elderly patients(≥65 years old)with gastric cancer after total gastrectomy.

关键词： albumin/globulin ratio neutrophil/lymphocyte ratio platelet/lymphocyte ratio gastric cancer prognosis disease-free survival overall survival cancer microenvironment gastrectomy

Cadmium(Cd)exposure through Hyphantria cunea pupae reduces the parasitic fitness of Chouioia cunea:A potential risk to its biocontrol efficiency

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Journal of Integrative Agriculture 2023年第10期22卷 3103-3114页

作者： YAN shan-chun WU Hong-fei ZHENG Lin TAN Ming-tao JIANG Dun School of Forestry Northeast Forestry UniversityHarbin 150040P.R.China Key Laboratory of Sustainable Forest Ecosystem Management Ministry of EducationNortheast Forestry UniversityHarbin 150040P.R.China

Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control,but the parasitic fitness of parasitoids under heavy metal stress is poorly ***,the effect of Cd exposure through the host pupa of Hyphantria cunea on the parasitic fitness of Chouioia cunea was investigated,and the mechanism by which Cd exposure affects the interaction between *** and *** from the perspective of innate immunity in host insect and the oxidative status in the parasitoid offspring was *** results indicated that Cd can be transferred from the *** pupae to the parasitoid offspring,and the transfer coefficient reflected biological *** were no significant differences in the rates of parasitism success and offspring emergence between the untreated and Cd-treated ***,after parasitizing Cd-accumulated pupae,the parasitic fitness of offspring wasps(e.g.,the number,individual size and life span)decreased *** Cd exposure,the cellular and humoral immunity of *** pupae decreased *** with the untreated group,the H_(2)O_(2)content of parasitoid offspring in the Cd-treated group was significantly *** exposure significantly inhibited superoxide dismutase activity in parasitoid offspring,but the contents of ascorbic acid and glutathione were significantly increased by Cd *** together,these results indicate that Cd exposure reduces the cyclic utilization efficiency of *** on *** *** oxidative status of parasitoid offspring triggered by Cd exposure could be responsible for the reduced parasitic fitness of *** on Cd-accumulated *** pupae.

关键词： heavy metal Hyphantria cunea parasitic fitness Chouioia cunea oxidative status innate immunity

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二维材料在火灾预警中的应用研究进展

现代化工 2024年第6期44卷 49-54页

作者：杨菲杜春贵王玉婷邵煜然尹文秀陈诗琴刘春林单莹颖浙江农林大学化学与材料工程学院浙江杭州311300

从预警机制、预警材料制备以及火灾预警性能等方面对GO基与MXene基火灾预警器的研究现状进行了综述,总结了以热致电导率变化为主的火灾预警机制和以火灾预警涂层、预警纸、预警膜、预警气凝胶等材料为主的预警模式,并对比分析了GO基与MX... 详细信息

从预警机制、预警材料制备以及火灾预警性能等方面对GO基与MXene基火灾预警器的研究现状进行了综述,总结了以热致电导率变化为主的火灾预警机制和以火灾预警涂层、预警纸、预警膜、预警气凝胶等材料为主的预警模式,并对比分析了GO基与MXene基2种不同基材火灾预警器在快速响应、重复响应、精准响应等预警性能方面的差异,最后对二维材料在火灾预警器中的未来发展方向进行了展望,以期为二维材料在火灾预警器中的研发与应用提供思路。

关键词：二维材料阻燃预警

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响应面法优化煤沥青基多孔电容炭的制备工艺

炭素技术 2024年第2期43卷 49-54页

作者：岳莉王伯琪单衍雪张文斌赖仕全朱亚明程均霞赵雪飞辽宁科技大学化学工程学院辽宁鞍山114051

煤沥青是制备超级电容器炭电极材料的优选前驱体。通过KOH活化从鞍钢中温煤沥青(AGCTP)制备多孔电容炭。在考察炭化温度、活化温度、碱炭比、活化时间、酸洗剂种类等单因素对比电容影响的基础上,通过Box-Behnken响应面法优化分析得到了... 详细信息

煤沥青是制备超级电容器炭电极材料的优选前驱体。通过KOH活化从鞍钢中温煤沥青(AGCTP)制备多孔电容炭。在考察炭化温度、活化温度、碱炭比、活化时间、酸洗剂种类等单因素对比电容影响的基础上,通过Box-Behnken响应面法优化分析得到了活化工艺条件与比容量间的二次回归模型。结果表明,原料沥青制备的多孔炭容量最大,而盐酸洗涤效果最佳。活化条件对比容量均有显著影响,得到的最优工艺条件:活化温度680℃、碱炭比2.4、活化时间2 h,在此条件所制电容炭在0.5 A/g电流密度下的比容量达418 F/g,与模型预测值基本吻合,且具有优异的倍率性和较小的电荷转移阻抗(0.17Ω)和内阻(0.72Ω)。

关键词：超级电容器煤沥青电容炭响应面工艺优化