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Two-dimensional crystallization of photosystem I chlorophyll a/b protein complex from cucumber

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Chinese Science Bulletin 1997年第7期42卷 597-601页

作者： LIU Zheng sui senfang WANG Shaoxiong LOU Shiqing WANG Kefen ZHAO Fuhong KUANG Tingyun Department of Biological Sciences and Biotechnology Tsinghua Univcrsity Beijing China Institute of Botany Chinese Academy of Sciences Beijing China

THE photosystem Ⅱ light-harvesting chlorophyll a/b protein complex (LHC Ⅱ) is a kind of integral membrane protein complex in the thylakoid membranes of chloroplasts from higher plants and green algae, and its most i... 详细信息

关键词： photosystem n light-harvesting chlorophyll a/b protein complex (LHC Ⅱ) two-dimensional crystallization electron crystallography biological diversity.

Regulatory dynamics of the higher-plant PSI-LHCI supercomplex during state transitions

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Molecular Plant 2023年第12期16卷 1937-1950页

作者： Jianghao Wu Shuaijiabin Chen Chao Wang Weijun Lin Chao Huang Chengxu Fan Dexian Han Dandan Lu Xiumei Xu senfang sui Lixin Zhang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityJinming AvenueKaifeng 475004China School of Life Science Southern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Membrane Biology Beijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureSchool of Life SciencesTsinghua UniversityBeijing 100084China Cryo-EM Center Southern University of Science and TechnologyShenzhen 518055China Photosynthesis Research Center Key Laboratory of PhotobiologyInsttute of BotanyChinese Academy of SciencesBejing 100093China

State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light *** process rebalances the excitation energy between photosystemI(PSl)and photosystem Il through regulated reversible binding of the light-harvesting complex Il(LHCll)to ***,the structural reorganization of PSI-LHCI,the dynamic binding of LHCll,and the regulatory mechanisms underlying state transitions are less understood in higher *** this study,using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1(PSI-LHCI-ST1)and state 2(PSILHCI-LHCll-ST2)from Arabidopsis *** genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCll trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO ***,PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCIl-ST2 supercomplex,in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ***,the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls,which may enhance the energy transfer efficiency from the peripheral antenna to the PSl core in ***,our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants.

关键词： state transitions dynamic conformation photosystem l regulatory mechanism Arabidopsis thaliana

Two forms of the membrane-bound state of the first C2 domain (C2A) of synaptotagminⅠand calcium-triggered membrane insertion

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Chinese Science Bulletin 2003年第17期48卷 1822-1827页

作者： HE Yuhong, LI Xianghui, WANG Fu, XUE Yi & sui senfang Department of Biological Sciences & Biotechnology, State Key Labora-tory of Biomembrane, Tsinghua University, Beijing 100084, China Department of Biological Sciences and Biotechnology State Key Laboratory of Biomembrane Tsinghua University Beijing 100084 China

The synaptic vesicle protein synaptotagminⅠ (sytⅠ) is a vesicle transmembrane protein present in synap-tic vesicles, which has been proposed as the Ca2+ sensor that regulates secretion. The C2A domain is the membrane proximal part of its cytoplasmic domain. The interaction between C2A and lipid bilayer has been considered to be essential for triggering neurotransmitter release. In the pre-sent work, the measurements of membrane surface tension and surface concentration showed that the C2A domain of sytⅠexhibited two membrane-bound states: the surface adsorption state and the membrane insertion state. The sur-face absorption state formed in a Ca2+-independent manner with lower affinity, while the membrane insertion state formed with high affinity was only found in the presence of Ca2+. Both the Ca2+-independent and Ca2+-dependent sytⅠ- membrane interactions required anionic phospholipids, such as phosphatidylserine (PS). When expressed into rat pheo-chromocytoma (PC12) cells and human embryonic kidney (HEK-293) cells, as demonstrated by immunofluorescence staining and subcellular fractionation, most of the C2A was found at the plasma membrane, even when the cells were depleted of Ca2+ by incubation with EGTA. These results suggested a new molecular mechanism of sytⅠas a Ca2+ sensor in membrane fusion. Ca2+-independent surface ad-sorption might attach sytⅠto the release site during the docking or priming step. When intracellular Ca2+ increased, sytⅠtriggered the neurotransmitter release following the Ca2+-dependent penetration into the target membrane.

关键词：突触小泡蛋白质膜贯通蛋白质钙离子薄膜插入 C2A 薄膜边界状态

Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural Science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, sui senfang and YANG Mengsu (State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China. Department of Chemistry College of Chemistry & Environmental Science Hebei University Baoding 071002 China. Department of Biology and Chemistry City University of Hong Kong Hong Kong China

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

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镁离子转运蛋白CorA间质结构域删除分析

清华大学学报（自然科学版） 2008年第6期48卷 1057-1061页

作者：孙占华王世珍黄萱许淼隋森芳清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室北京100084

为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌Cor... 详细信息

为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌CorA转运活性测定体系。结果显示:大肠杆菌CorA间质结构域N-端起始的24个残基对于CorA在酵母质膜中的表达或维持其本身正确构象发挥重要作用,CorA M124到D154段的部分残基在CorA介导的镁离子转运过程中起到重要作用。

关键词：分子生物物理学镁离子转运系统钴离子抗性等位基因(CorA) 间质结构域删除分析

参与唇腭部发育的重要蛋白复合体ESCRTⅢ的单分子检测体系的构建

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中华口腔正畸学杂志 2019年第3期26卷 152-156页

作者：李瑶杨梦铱李琳陈春来隋森芳孙珊生物膜国家重点实验室结构生物学高精尖创新中心清华大学生命科学学院北京100084 结构生物学高精尖创新中心清华大学生命科学学院北京100084

目的构建参与唇腭部发育的重要蛋白ESCRTⅢ的单分子检测体系。方法分析ESCRTⅢ蛋白的结构,选取不影响结构稳定和纤丝形成的氨基酸进行突变,体外提取突变蛋白检测蛋白的稳定性,吸收光谱检测突变蛋白荧光标记的效率,负染电镜观察突变蛋白... 详细信息

目的构建参与唇腭部发育的重要蛋白ESCRTⅢ的单分子检测体系。方法分析ESCRTⅢ蛋白的结构,选取不影响结构稳定和纤丝形成的氨基酸进行突变,体外提取突变蛋白检测蛋白的稳定性,吸收光谱检测突变蛋白荧光标记的效率,负染电镜观察突变蛋白形成纤丝的能力,负染电镜观察和离心沉淀分析突变体蛋白形成的纤丝被Vps4解聚的能力。结果选取了ESCRTⅢ蛋白Snf7上的5个位点,K57,G58,N59,G120和D122,分别突变为半胱氨酸,其中K57C突变体蛋白不稳定聚沉,G58C突变体蛋白荧光标记效率低,N59C和G120C突变体蛋白影响纤丝形成,只有D122C突变体蛋白稳定,标记效率高,且标记后的蛋白能正常形成纤丝并被Vps4蛋白解聚。结论筛选出了Snf7 D122C突变体适用于利用单分子技术检测ESCRTⅢ纤丝的解聚动态过程,从而为进一步在细胞水平和体内研究ESCRT系统对腭部发育的作用提供支持。

关键词：唇腭裂 Snf7 ESCRTⅢ 单分子检测 Vps4

Molecular regulation of mitochondrial autophagy in mammalian cells

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Molecular regulation of mitochondrial autophagy in mammalian...

用椭圆偏振术研究血清白蛋白和纤维蛋白原在硅片及聚氨酯薄膜表面的吸附行为

生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： Lei Liu Du Feng Ming Chen Guo Chen Qiaoxia Zheng Pingping Song Qi Ma Chongzhuo Zhu Lei Huang Baowei Li Xiaohui Wang Haijing Jin Jun Wang Yushan Zhu senfang sui Quan Chen Laboratory of Apoptosis and Cancer Biology The State Key Laboratory of Biomembrane and Membrane BiotechnologyInstitute of ZoologyChinese Academy of Sciences Graduate School of the Chinese Academy of Sciences College of Life Sciences Nankai University College of Life Sciences Tsinghua University Center of Biomedical Analysis Tsinghua University

Mitochondria are major source for free radical generation in addition to its functions in cell death and energy *** of damaged mitochondria is harmful to the cell and has been related with a number of diseases including neurodegenerative diseases,diabetes and ***,it has been shown that dysfunctional mitochondria can be selectively removed by a mechanism of mitochondrial autophagy,or *** molecular regulation of this critical process in mammalian cells remains poorly understood.

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中国生物医学工程学报 1998年第1期17卷 54-58页

作者：胡勇曾雪梅公衍道隋森芳赵南明清华大学生物科学与技术系

用椭圆偏振术研究了牛血清白蛋白（ＢＳＡ）和人纤维蛋白原（ＦＧＮ）在亲水硅片表面上的吸附行为。结果表明：蛋白质浓度大于５μｇ／ｍｌ时，相同蛋白质浓度下，ＢＳＡ的最终吸附量小于ＦＧＮ。在蛋白质浓度变化的情况下，ＢＳＡ在吸... 详细信息

用椭圆偏振术研究了牛血清白蛋白（ＢＳＡ）和人纤维蛋白原（ＦＧＮ）在亲水硅片表面上的吸附行为。结果表明：蛋白质浓度大于５μｇ／ｍｌ时，相同蛋白质浓度下，ＢＳＡ的最终吸附量小于ＦＧＮ。在蛋白质浓度变化的情况下，ＢＳＡ在吸附液蛋白质浓度约为１０μｇ／ｍｌ时即达到饱和吸附（即最终吸附量不再随蛋白浓度提高而增加），而ＦＧＮ达到饱和吸附时的吸附液浓度约为２０μｇ／ｍｌ。ＢＳＡ和ＦＧＮ吸附液浓度同为３０μｇ／ｍｌ时，ＢＳＡ的吸附速率及饱和吸附量均大大低于ＦＧＮ。对于同一蛋白质ＦＧＮ，吸附液浓度３０μｇ／ｍｌ时的吸附速率和饱和吸附量均明显大于浓度为７．５μｇ／ｍｌ时的吸附速率和饱和吸附量。用椭圆偏振术研究高分子材料表面蛋白质的吸附行为，主要问题是如何使生物材料表面达到椭圆偏振测量所要求的高光洁度和高反射率。本文发展了一种在抛光硅片基底上铺展高分子材料薄膜的方法，制备的样品符合椭圆偏振测量的要求。应用椭圆偏振术初步研究了ＦＧＮ在聚氨酯表面的吸附行为，并与ＦＧＮ在亲水硅片上的吸附行为进行了初步比较。结果表明，ＦＧＮ在聚氨酯表面上的吸附速率和饱和吸附量均大大低于该蛋白质在亲水硅片表面上的吸附。提示亲水硅片的血液相容性比聚氨酯差得?

关键词：椭圆偏振蛋白吸附硅片聚氨酯纤维蛋白原 BSA

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红藻藻胆体的结构及关键色素分析

自然杂志 2021年第3期43卷 176-188页

作者：肖亚男马建飞游鑫隋森芳南方科技大学生命科学学院广东深圳518055 清华大学生命科学学院北京100084

藻胆体是红藻和蓝藻中的大型水溶性捕光复合体,能吸收较宽范围波长的可见光,能量传递效率高于95%。目前解析的高分辨率藻胆体来自红藻Griffithsia pacifica(太平洋凋毛藻)和Porphyridium purpureum(紫球藻),在这两个物种中获得了藻胆体... 详细信息

藻胆体是红藻和蓝藻中的大型水溶性捕光复合体,能吸收较宽范围波长的可见光,能量传递效率高于95%。目前解析的高分辨率藻胆体来自红藻Griffithsia pacifica(太平洋凋毛藻)和Porphyridium purpureum(紫球藻),在这两个物种中获得了藻胆体完整的蛋白结构,确定了所有连接蛋白的结构和分布,并且在Porphyridium purpureum藻胆体的结构中发现连接蛋白具有调节藻胆蛋白色素能量状态的作用。文章将针对藻胆体的整体结构和关键色素的微环境进行分析。

关键词：藻胆体连接蛋白色素能量传递光合作用