Regulatory dynamics of the higher-plant PSI-LHCI supercomplex during state transitions
作者机构：State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityJinming AvenueKaifeng 475004China School of Life ScienceSouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Membrane BiologyBeijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureSchool of Life SciencesTsinghua UniversityBeijing 100084China Cryo-EM CenterSouthern University of Science and TechnologyShenzhen 518055China Photosynthesis Research CenterKey Laboratory of PhotobiologyInsttute of BotanyChinese Academy of SciencesBejing 100093China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第12期
页 面：1937-1950页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：the National Key Research and Development Program of China(2020YFA0907600 to L.Z.) the National Natural Science Foundation of China(National Science Foundation of China)(32241030 to S.S.)
主 题：state transitions dynamic conformation photosystem l regulatory mechanism Arabidopsis thaliana
摘 要：State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light *** process rebalances the excitation energy between photosystemI(PSl)and photosystem Il through regulated reversible binding of the light-harvesting complex Il(LHCll)to ***,the structural reorganization of PSI-LHCI,the dynamic binding of LHCll,and the regulatory mechanisms underlying state transitions are less understood in higher *** this study,using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1(PSI-LHCI-ST1)and state 2(PSILHCI-LHCll-ST2)from Arabidopsis *** genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCll trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO ***,PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCIl-ST2 supercomplex,in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ***,the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls,which may enhance the energy transfer efficiency from the peripheral antenna to the PSl core in ***,our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants.
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