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Plant Diversity 2024年第4期46卷 435-447页

作者： Jun-Yi Zhang Yue-Hong Cheng Min Liao Yu Feng sen-Long Jin Ting-Mei He Hai He Bo Xu China-Croatia“Belt and Road”Joint Laboratory on Biodiversity and Ecosystem Services CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan ProvinceChengdu Institute of BiologyChinese Academy of SciencesP.O.Box 416Chengdu 610041SichuanChina College of Life Sciences Chongqing Normal UniversityChongqing 401331China Wolong National Natural Reserve Administration Bureau Wenchuan 623006SichuanChina University of Chinese Academy of Sciences Beijing 100049China

Gastrochilus is an orchid genus containing 73 species of mainly epiphytic on trees or rocks in mountain forests of tropical and subtropical *** phylogenetic analyses and morphological assessments have failed to produce a well-resolved phylogeny at the infrageneric *** the present study,a new infrageneric classification of Gastrochilus is proposed based on thoroughly morphological and phylogenetic analyses based on 52 *** phylogenetic analysis divided the genus into six sections including three new sections,***,*** and *** also reinstate *** to the specific ***,two new species,*** Jun ***,***&Yue *** and *** Jun ***,***&Yue ***,are described and illustrated.A key to six sections of the genus is presented.

关键词： Gastrochilus Phylogeny Character evolution Taxonomy New species

Dual Differential Thermal Analysis and Instrument

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Chinese Chemical Letters 1997年第6期8卷 527-528页

作者： Zuan Guang CHEN Peng LIU, Min Sheng ZRANG (Department of Chemistry, Sun Yat-sen University of Medical Sciences, Guanghou 510089) Department of Chemistry Sua Yat-sen Univ. Medical Sciences Guangzho 510089

A new method of differential thermal analysis and instrument were developed. The solution and pure solvent were placed in a cooling bath whose temperature was decreased by a semiconductor chip. Three detectors were used to sense the temperature (Ts, Tr and Tw) of solution (S), pure solvent (R) and cooling bath (W). When (Tr-Ts)-(Ts-Tw)presented maximum, (Tr-Ts) was the freezing point depression of the solution which was proportional to the concentration of solution. An instrument for the method was designed.

关键词： Ts AR AT

Risk factors and metabolic abnormality of patients with non-alcoholic fatty liver disease: Either non-obese or obese Chinese population

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Hepatobiliary & Pancreatic Diseases International 2018年第1期17卷 45-48页

作者： Shou-Wu Lee Teng-Yu Lee Sheng-Shun Yang Chun-Fang Tung Hong-Zen Yeh Chi-sen Chang Division of Gastroenterology Department of Internal Medicine Taichung Veterans General Hospital

Background: Non-alcoholic fatty liver disease(NAFLD) occurs not only in obese individuals but also in non-obese ones. The aim of this study was to focus on the association between NAFLD and metabolic events in a non-obese or obese Chinese ***: Data collected from subjects registered at Taichung Veterans General Hospital from January to December 2009 were analyzed. The exclusion criteria were alcoholics, chronic hepatitis B or C. Patients included in analyses were assigned to four groups according to sonography of their liver(normal or NAFLD), and body mass index(BMI) levels(non-obese if BMI < 25 kg/m~2 or obese if BMI ≥ 25 kg/m~2).Results: There were 745, 208, 770 and 285 patients enrolled in four groups labeled non-obese normal liver(group A), non-obese NAFLD(group B), obese normal liver(group C) and obese NAFLD(group D),respectively. The highest ratio of metabolic syndrome existed in the group B(26.9%), followed by group A(11.7%), group D(10.9%) and finally the group C(5.2%). The positive association with NAFLD in non-obese individuals was significant in triglyceride(OR = 1.01; 95% CI: 1.01–1.02) and glucose(OR = 1.02; 95% CI:1.01–1.03), while the positive association with NAFLD in obese subjects was only significant in triglyceride(OR = 1.01; 95% CI: 1.01–1.02). The positive association was most significant in all cases(adjusted OR = 2.41; 95% CI: 1.78–3.24), especially in non-obese individuals(OR = 2.81; 95% CI: 1.92–4.12).Conclusions: Non-obese NAFLD subjects displayed a higher proportion of metabolic abnormality. Hyperlipidemia and hyperglycemia had the most positive strength association with NAFLD.

关键词： Fatty liver Non-alcoholic fatty liver disease Obese Chinese population

Influence of Yb_2O_3-MgO on Mechanical Properties and Thermal Conductivity of Silicon Nitride Ceramics via Gas Pressure Sintering

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China's Refractories 2015年第3期24卷 34-39页

作者： LIN sen YAO Dongxu XIA Yongfeng ZUO Kaihui YIN Jinwei ZENG Yuping State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of Sciences Graduate School of Chinese Academy of Sciences

In this work,Yb2O3 and Mg O were used as sintering aids in preparing silicon nitride ceramics by gas pressure sintering（ 0. 6 MPa N2atmosphere） to investigate how the amounts of Yb2O3- Mg O influence the mechanical properties and thermal conductivity of silicon nitride ceramics. The total contents of Yb2O3- Mg O added were 1 mol%,2 mol%,4 mol%,6 mol%,8 mol%,10 mol%,12 mol%,14 mol%,keeping the Yb2O3-Mg O molar ratio of 1 ∶ 1 steadily. Curves of the relative density,thermal conductivity and bending strength plotted against the aids content present a ‘mountain＇shape with a maximum at nearly 10 mol% aids. The fracture toughness increased with the amounts of additives up to10 mol% and decreased slightly thereafter. The mechanical properties and thermal conductivity were almost proportional to the amount of the additives before10 mol%. When the content of aids exceeded 10 mol%,it would weaken the mechanical properties and thermal conductivity of the ceramics. The optimum content of Yb2O3- Mg O was 10 mol% by gas pressure sintering（ 0. 6MPa） at 1 850 ℃ for 4 h,which led to a relative density of 98. 9%,a flexural strength of（ 966 ± 38）MPa as well as a fracture toughness of（ 6. 29 ± 0. 29）MPa·m1 /2and thermal conductivity of 82 W /（ m·K）.

关键词： silicon nitride sintering mechanical properties thermal conductivity

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增强蜂窝结构面内力学行为

塑性工程学报 2023年第11期30卷 131-140页

作者：乔及森祝伟王磊王晨洋兰州理工大学材料科学与工程学院甘肃兰州730050 兰州理工大学省部共建有色金属加工与再利用国家重点实验室甘肃兰州730050

为了提高蜂窝结构的面内吸能性和承载能力,通过在铝蜂窝孔中添加不同类型的加强筋板,设计了6种增强蜂窝结构。通过非线性有限元计算模拟的方法对比分析了在相同相对密度、面内不同方向加载和不同冲击速度下,增强蜂窝结构的动态响应和能... 详细信息

为了提高蜂窝结构的面内吸能性和承载能力,通过在铝蜂窝孔中添加不同类型的加强筋板,设计了6种增强蜂窝结构。通过非线性有限元计算模拟的方法对比分析了在相同相对密度、面内不同方向加载和不同冲击速度下,增强蜂窝结构的动态响应和能量吸收特性。模拟结果表明,增强蜂窝结构可以在低速冲击下表现出传统蜂窝结构高速冲击下的逐层压溃现象,使蜂窝材料获得更高的平台应力。增强结构对蜂窝材料面内强化效果显著不同,加强筋板结构可以使蜂窝结构获得更好的吸能性,与传统蜂窝相比,特别是低速冲击状态下,增强蜂窝结构相较于同等质量下的均匀蜂窝结构,比吸能最高可以提升54.42%,表明加强筋板结构可以有效提升蜂窝的抗冲击性能和能量吸收能力。

关键词：蜂窝结构面内性能有限元计算增强结构

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Analysis of a case of corticobasal degeneration started with primary progressive aphasia

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China Medical Abstracts(Internal Medicine) 2022年第2期39卷 124-125页

作者： HUANG sen Dept Neurol 1st Affil HospSun Yat-sen UnivGuangdong Prov Key Major Neurol Dis Diag&TreatLab Key ClinGuangzhou 510080

Objective Through a typical case of corticobasal degeneration (CBD) with primary progressive aphasia(PPA) to analyze the clinical characteristics of CBD and the special manifestations of aphasia with that *** Retrospective analysis was performed on a patient with PPA based CBD who was admitted to the First Affiliated Hospital, Sun Yat-sen University in July 2020to summarize the clinical features and diagnostic thinking of CBD.

关键词： degeneration admitted clinical

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回转内圆柱面的非拼接干涉测量方法

红外与激光工程 2017年第7期46卷 187-193页

作者：孙昊韩森唐寿鸿王芳长春理工大学光电工程学院吉林长春130022 上海理工大学光电信息与计算机工程学院上海200093 苏州慧利仪器有限责任公司江苏苏州215123

介绍了一个特殊结构的干涉测试方法和系统,基于Fizeau(菲索)干涉原理,通过一高精度直角圆锥镜来实现圆柱坐标系与直角坐标的转换。该系统可以用来测量360°回转圆柱的表面形貌,并且无需图像拼接,可一次性获取整周的面形数据。通过对系... 详细信息

介绍了一个特殊结构的干涉测试方法和系统,基于Fizeau(菲索)干涉原理,通过一高精度直角圆锥镜来实现圆柱坐标系与直角坐标的转换。该系统可以用来测量360°回转圆柱的表面形貌,并且无需图像拼接,可一次性获取整周的面形数据。通过对系统主成分误差的计算分析,及Matlab软件数值仿真,建立了圆柱坐标系下的误差校正矩阵。并针对一环形样品进行实际测量,测试结果经过主成分误差校正后,与Taylor Hobson接触式圆柱度仪的测量结果一致,通过最小二乘圆(LSC)的圆度评定方法计算得到的圆度误差值十分接近。实验结果表明:该测量方法可有效地实现圆柱体整周表面形貌的测量,解决了目前回转光学元件的测量难度大、测试效率低等问题。

关键词： Fizeau干涉仪回转圆柱面角锥反射镜误差校正

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 479-482页

作者： Bao-zhong ZHANG Xin ZHANG Xiao-ping AN Duo-liang RAN Yu-sen ZHOU Jun LU Yi-gang TONG State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China College of Veterinary Science Xinltang Agricultural University Urumqi 830052 China Tumor Biotherapy Wurd Beijing YouAn Hospital Capital Medical University Beijing 100069 China

Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.

关键词： Site-directed mutagenesis (SDM) Restriction endonuclease Mutant screening

Effects of Nitrogen and Phosphorus Fertilization on Soil pH-Plant Productivity Relationships in Upland Grasslands of Northern Greece

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Pedosphere 2011年第6期21卷 750-752页

作者： S.D.VERESOGLOU O.K.VOULGARI R.sen A.P.MAMOLOS D.S.VERESOGLOU Faculty of Agriculture Laboratory of Ecology and Environmental ProtectionAristotle University of Thessaloniki Department of Environmental and Geographical Sciences Manchester Metropolitan UniversityJohn Dalton Building

The potential role of soil pH in modulating plant productivity was assessed on the basis of dry mass, harvested in the form of 0.25 m2 quadrats, in two low-productivity upland grasslands of northern Greece subjected to annual factorial nitrogen （N） and phosphorus （P） fertilization （15 g N m-2 year-1 and 10 g P m-2 year-I） over a minimum period of 3 years. It was hypothesized that under these particular conditions, a positive relationship would exist between soil pH and plant productivity and, further, that N or P fertilizer application of a nutrient limited system, would result in a weakening of this positive relationship. A significant positive relationship was confirmed between soil pH and plant productivity in one of the two study areas and a positive trend in the other. Moreover, plant productivity increase, following fertilization, appeared to have a detrimental effect on the soil pH-plant productivity relationship. Findings support the original hypotheses and strengthen the idea that plant diversity-mediated soil pH-plant productivity relationships are a result of the more intense plant speciation that occurred under the more ＂typical＂ soil conditions of higher pH in temperate as opposed to tropical regions

关键词： altitude ecological patterns low productivity grasslands temperate ecosystems

NLRP3 Deficiency Attenuates Secondary Degeneration of Visual Cortical Neurons Following Optic Nerve Injury

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Neuroscience Bulletin 2020年第3期36卷 277-288页

作者： Zhou Zhang Wenyi Liu Yubin Huang Linlin Luo Xiaofeng Cai Yunjia Liu Liqianyu Ai Jun Yan sen Lin Jian Ye Department of Ophthalmology Research Institute of Surgery and Daping HospitalArmy Medical Center of the People’s Liberation Army(PLA)Army Medical UniversityChongqing 400042China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518000China Department 1 Research Institute of Surgery and Daping HospitalArmy Medical Center of the PLAArmy Medical UniversityChongqing 400042China

In the visual pathway, optic nerve(ON) injury may cause secondary degeneration of neurons in distal regions, such as the visual cortex. However, the role of the neuroinflammatory response in regulating secondary impairment in the visual cortex after ON injury remains unclear. The NOD-like receptor family pyrin domain containing 3(NLRP3) is an important regulator of neuroinflammation. In this study, we established a mouse model of unilateral ON crush(ONC) and showed that the expression of NLRP3 was significantly increased in the primary visual cortex(V1) as a response to ONC and that the NLRP3 inflammasome was activated in the contralateral V1 1 days–14 days after ONC. Ablation of the NLRP3 gene significantly decreased the trans-neuronal degeneration within 14 days. Visual electrophysiological function was improved in NLRP3-/- mice. Taken together, these findings suggest that NLRP3 is a potential therapeutic target for protecting visual cortical neurons against degeneration after ON injury.

关键词： NLRP3 Visual cortex Optic nerve injury Visual cortical degeneration