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Chinese Chemical Letters 1999年第1期10卷 55-58页

作者： Qin Hua RU Yi Ming WANG Guo An LUO(Department of Chemistry. school of life science and engineering,Tsinghua University. Beijing, 100084) Department of Chemistry Sch. of Life Science and Engineering Tsinghua University Beijing 100084 China

Guillain-Barre syndrome (GBS) is considered to be an autoimmune disorder of peripheralnervous system. In this paper. capillary SDS gel electrophoresis was performed on neutral coatedfused-silica capillary to determine... 详细信息

关键词： Immunoglobulin G Guillain-Barre syndrome Capillary SDS gel electrophoresis Molecular weight of protein

Interaction between N-Phospho-Amino Acids and Nucleoside in Aqueous Medium

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Chinese Chemical Letters 2000年第5期11卷 407-408页

作者： Yu Fen ZHAO Jian Jun HU Yong JU (State Laboratory of Bioorganic Phosphorus Chemistry. Department of Chemislry. school of life science and engineering. Tsinghua University. Beijing 100084) Tsing Hua Univ Sch Life Sci & Engn Dept Chem State Lab Bioorgan Phosphorus Chem Beijing 100084 Peoples R China

Nucleosides were phosphorylated with different N- (O, O-diisopropyl) phosphoryl amino acids to give nucleoside mono phosphates in aqueous solution. 2', 3', and 5'-isomers had been confirmed by comparison with authentic samples on the basis of HPLC analysis. The conversion percentage of nucleoside indicated that N- (O, O-diisopropyl) phosphoryl aspartic acid reacted with adenosine and guanosine at a much higher rate than other kinds of N- phosphoryl amino acids. while phosphorylation of cytidine and uridine was relatively easy by using N- (O, O-diisopropyl) phosphoryl threonine. The result could give some clue to the prebiotic code origin of nucleic acid and protein.

关键词： N-phospho-amino acids nucleoside mono phosphate phosphorylation uridylyl uridine (UpU) origin chemical evolution

The Effects of Axial-Flow Pump in Supporting of Regional Ischemic Left Ventricle: A Simulation Study

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Chinese Journal of Biomedical engineering(English Edition) 1999年第3期8卷 46-47页

作者： Bing Zhao and Jing Bai(Department of Electrical engineering, The school of life science and engineering,Tsinghua University, Beijing 100084, China)

Formation　of　Dithymidylic　Acid　by　Ester　Exchange　Reaction　on－Phosphoryl　Serine

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Chinese Chemical Letters 1996年第10期7卷 905-906页

作者： Yuan MA Xiang Hong LI Yi CHEN Yu Ping FENG Yu Fen ZHAO(Department of Chemistry Bio-organic Phosphorus Chemistry Laboratory,school of life science and engineering Tsinghua University, Beijing, 100084)(Institute of Chemistry, Chinese Academy of sciences Dept. Chem. Bio-Organ. Phosphorus C. School of Life Science Engineering Tsinghua University Beijing 100084 Institute of Chemistry Chinese Academy of Sciences Beijing 100080

Ester exchange reaction between the thymidine and N(O,O-diisopropyl)phosphoryl serine could yield several derivatives of thymidylic acid, including dithymidylic acid. These results might be meaningful to the prebiotic... 详细信息

关键词： Acid Ester Dithymidylic Exchange Reaction

A general mechanism for β-lactam and protein acetylation revealed by the crystal structure of Tabtoxin resistance protein complexed with acetyl coenzyme A

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A general mechanism for β-lactam and protein acetylation re...

第九次全国生物物理大会学术会议

作者： Zihe Rao Laboratory of Structural Biology and the MOE Laboratory of Protein science, school of life science & engineering, Tsinghua University Beijing 100084, China.

＜正＞ Tabtoxin resistance protein （TTR） is an enzyme that renders Tabtoxin-producing pathogens, such as Pseudomonas syringae, tolerant to their own phytotoxins. Here we report the crystal structure of TTR complexed with its natural substrate, acetyl coenzyme A （AcCoA）, to 1.55 A resolution. Despite low sequence homology, the single domain protein includes the four motifs conserved in the GCN5-related N-acetyltransferase （GNAT） superfamily, as well as a characteristic ’V’ shape in which AcCoA binds between the two arms of the ’V’. We propose a single-step mechanism through which TTR catalyses the inactivation of Tabtoxin initiated by the transfer of an acetyl group to the toxin. Our structure provides new insight into the understanding of the role of three critical residues that are conserved in the catalytic pocket of many GNAT superfamily members, including the histone acetyltransferases. We further report that TTR possesses histone acetyltransferase activity and, therefore, describe a general catalytic mechanism for acetylation of β-lactam and protein.

关键词： Tabtoxin resistance protein acetyl coenzyme A crystal structure catalytic mechanism GCN5-related N-acetyltransferase superfamily histone acetyltransferase activity

Derivative Synthesis of Wanpeinine A,a Major Steroidal Alkaloid from Fritillaria shuchengensis

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Chinese Herbal Medicines 2010年第2期2卷 141-144页

作者： WEN Juan,ZHOU Xian-li,YAN Ren-long,HUANG Shuai,WANG Yin-hui school of life science and engineering,Southwest Jiaotong University,Chengdu 610031,China school of life science and engineering Southwest Jiaotong UniversityChengdu 610031China

Objective To design and synthesize derivatives of wanpeinine A,the main steroidal alkaloid isolated from the plant Fritillaria shuchengensis,and further study on the structure-activity relationship of the steroidal *** Acylation and alkylation were used to synthesize the derivatives and their structures were identified via NMR and *** The acylation of wanpeinine A(1) produced 3β,6α-diacetylwanpeinine A(2),3β,6α-dipropionyl-wanpeinine A(3),3β,6α-dichloracetylwanpeinine A(4),3β,6α-dibenzoylwanpeinine A(5),and 3β-methoxy-acylwanpeinine A(6).The alkylation of wanpeinine A formed 3β,6α-dimethoxymethylwanpeinine A(7).Conclusion All compounds are new except for 3β,6α-diacetylwanpeinine A.

关键词： Fritillaria shuchengensis steroid alkaloid wanpeinine A

MODELING KINASE-SUBSTRATE SPECIFICITY IMPLICATION OF REACTION COORDINATE DISTANCE BETWEEN SUBSTRATE NUCLEOPHILIC OXYGEN AND ATTACKED PHOSPHORUS OF ATP ANALOG ON BINDING AFFINITY

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MODELING KINASE-SUBSTRATE SPECIFICITY IMPLICATION OF REACTIO...

第六届全国磷化学化工学术讨论会

作者： Ming Sun, Xiao-HongLiu and Yu-fen Zhao The Key Laboratory of Bioorganic Phosphorous Chemistry,Ministry of education,China Department of Chemistry,school of life science and engineering,Tsinghua University,Beijing 100084,China, school of Chemistry and life science,Tianjin Normal University,Tianjin 300074,China

Protein kinases play a critical role in cell signaling pathways by catalyzing the transfer of the α-phosphoryl group from ATP to the hydroxyl group of substrate side chain. This process affects essentially every cellular process including metabolism, growth, differentiation, motility, membrane transport, learning and memory. To ensure the signaling fidelity, the kinases must be specific and act only on a defined subset of cellular targets. Understanding the basis for this substrate specificity of different protein kinases is therefore

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Study on the Synthesis and Characterization of Phosphopeptides

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Study on the Synthesis and Characterization of Phosphopeptid...

第六届全国磷化学化工学术讨论会

作者： Yan-Mei LI & Yu-Fen Zhao Bioorganic Phosphorus Chemistry Laboratory,Department of Chemistry,school of life science and engineering,Tsinghua University,Beijing 100084,CHINA

The reversible phosphorylation/dephosphorylation ot protein is probably me most common ana important regulatory modification of *** is involved in the regulation of gene expression and protein synthesis that controls cell growth,division,or differentiation (1-3).For the study of the events influenced by phosphorylated proteins,phosphopeptides that embody the characteristic phosphorylated partial structures of the parent proteins have proven to be invaluable reagents (5).So the development of methods for their synthesis is the subject of various research enterprises (6-8).

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New Eremophilane Derivatives From Senecio dianthus

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New Eremophilane Derivatives From Senecio dianthus

中国化学会第9届天然有机化学学术会议

作者： Shuai Huang,Jing Huang,Hui Li,Xiao-li Zhou,Xian-li Zhou~* school of life science and engineering,Southwest Jiaotong University,Chengdu 610031,Sichuan,.China.

＜正＞Senecio dianthus *** to the Asteraceae family,is herbaceous plant that is widely distributed in China,mostly distributed in Tibet and reputed for reducing fever and detoxification in Tibetan herbal *** chemical components had been investigated by Yan-Hui Li ***,which led to the identification of seven eremophilenolides and eremophilenolide *** this paper,five new eremophilane glucosides（1-5） and one new eremophilane（6）（Figure 1）,together with sixteen known compounds were isolated from this

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酸性多糖的最新研究进展

食品科技 2010年第5期35卷 80-83页

作者：熊双丽李安林西南科技大学生命科学与工程学院绵阳621010

酸性多糖是目前发现的分布和应用最广、生物活性最多、制备和结构鉴定最为复杂的一类生物活性多糖。主要详述其提取、分离纯化、生物活性、结构鉴定及应用方面的最新研究动态和发展趋势。

关键词：酸性多糖分离纯化生物活性结构鉴定