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Nucleotide Sequence and characterization of Bovine Metallothionein Gene

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chinese Science Bulletin 1993年第5期38卷 419-423页

作者：杨靖沈孝宙杨卫民 M.G.Peterson J.F.B.Mercer savio l.c.woo Institute of Developmental Biology Academia Sinica Beijing 100080 PRC. laboratory of Reproductive Biology Institute of Zoology Academia Sinica Beijing 100080 PRC Birth Defects Research Institute Poyal Children's Hospital Parkville Australia Department of cell Biology Baylor College of Medicine Houston TX 77030 USA

1 Introduction Metallothioneins (MTs) are a family of low-molecular weight heavy metal binding proteins, unique in their high cystein content. MTs are ubiquitously distributed in the eukaryote from yeast to human. It is an experimental model emphasized during researching gene expression and regulation of animal cells. In order to provide inducible regulatory elements for transgenosis livestock, we cloned bovine MT genes. At least four members of bovine MT family have been discovered. In this note, one gene (bMTc) of

关键词： bovine metallothionein gene DNA sequence gene structure.

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牛金属硫蛋白基因的克隆及其组织结构的研究

科学通报 1988年第16期33卷 1267-1269页

作者：沈孝宙,杨卫民,彭红,M.G.Peterson,J.F.B.Mercer,savio l.c.woo 中国科学院动物研究所生殖生物学开放研究实验室中国科学院动物研究所生殖生物学开放研究实验室中国科学院动物研究所生殖生物学开放研究实验室澳大利亚皇家儿童医院澳大利亚皇家儿童医院美国贝勒医学院细胞生物系北京北京北京帕克威利帕克威利休斯敦

金属硫蛋白(Metallothionein。简称MT)是广泛分布在动植物界的一组富含半胱氨酸的蛋白,能特异结合重金属,如镉、锌、汞和铜等。在生物体内,重金属离子可诱导MT基因的表达。1982年美国Palmiter等人首次成功地获得高效表达外源生长激素的... 详细信息

金属硫蛋白(Metallothionein。简称MT)是广泛分布在动植物界的一组富含半胱氨酸的蛋白,能特异结合重金属,如镉、锌、汞和铜等。在生物体内,重金属离子可诱导MT基因的表达。1982年美国Palmiter等人首次成功地获得高效表达外源生长激素的“基因移植”

关键词：牛金属硫蛋白基因基因的组织结构基因组克隆

Tetramic acid-motif natural products from a marine fungus Tolypocladium cylindrosporum FB06 and their anti-Parkinson activities

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Marine life Science & Technology 2024年第1期6卷 84-92页

作者： Yuna Jung chaesun Kwon Taeyeon Kim Jin woo lee Min-Kyoo Shin Sang Hee Shim Natural Products Research Institute College of PharmacySeoul National UniversitySeoul 08826Republic of Korea college of Pharmacy and Research Institute of Pharmaceutical Sciences Seoul National UniversitySeoul 08826Republic of Korea college of Pharmacy Duksung Women’s UniversitySeoul 01369Republic of Korea

Tetramic acid-containing natural products are attracting significantly increasing attention from biologists and chemists due to their intriguing structures and biological *** the present study,two new tetramic acid alkaloids tolypyridone I(1)and tolypyridone J(2),together with five known ones(3–7),were isolated from cultures of a marine fungus Tolypocladium cylindrosporum FB06 isolate obtained from a marine sediment in Beaufort sea of North *** structures were elucidated using 1D,2D NMR,and *** configurations were established on the basis of 1H coupling constants,ROESY correlations and DP4 *** 2 was isolated as mixtures of rotational isomers with c-3 to c-7 axis between 4-hydroxy-2-pyridone and 1-ethyl-3,5-dimethylcyclohexane,hindering *** our unbiased screening to discover neuroprotective compounds in an in vitro Parkinson’s disease(PD)model,SH-SY5Y dopaminergic cells were treated with isolated compounds followed by treatment with 1-methyl-4-phenylpyridinium(MPP^(+)),a parkinsonian *** tested compounds,F-14329(7)significantly protected cells from MPP^(+)-induced ***^(+)-mediated cell death is known to be related to the regulation of Bcl-2 family proteins,specifically the down-regulation of anti-apoptotic Bcl-2 and the up-regulation of pro-apoptotic Bax *** with 2 mmol/l of MPP^(+)for 24 h significantly reduced Bcl-2 levels compared to control treated with ***,treatment with F-14329(7)attenuated such *** study demonstrates that tetramic acid-motif compounds could be potential lead compounds for treating PD.

关键词： Tolypocladium cylindrosporum Tetramic acid Tolypyridone Parkinson’s disease

The Use of the Matrix Method for the Study of Human Motion:Theory and Applications

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生物医学工程学杂志 2003年第3期20卷 375-383页

作者： Jesse A.Fisk savio l-Y.woo Musculoskeletal Research center Department of Orthopaedic SurgeryUniversity of PittsburghMusculoskeletal Research CenterDepartment of Orthopaedic SurgeryUniversity of Pittsburgh PA 15213USAPA 15213USA

Kinematics has been successfully used to describe body motion without reference to the kinetics (or forces causing the motion). In this article, both the theory and applications of the matrix method are provided to describe complex human motion. After the definition of a cartesian coordinate frame is introduced, the description of transformations between multiple coordinate frames is given; the decomposition of a transformation matrix into anatomical joint motion parameters (e.g. Euler angles) is then explained. The advantages of the matrix method are illustrated by three examples related to biomechanical studies. The first describes a reaching and grasping task in which matrix transformations are applied to position the hand with respect to an object during grasping. The second example demonstrates the utility of the matrix method in revealing the coupling motion of the wrist between flexion-extension and radial-ulnar deviation. The last example highlights the indispensable use of the matrix method for the study of knee biomechanics, including the description of knee joint kinematics during functional activities and determination of in-situ ligament forces using robotic technology, which has advanced our understanding of the functions of the cruciate ligaments to knee joint kinematics. It is hoped that the theoretical development and biomechanical application examples will help the readers apply the matrix method to research problems related to human motion.

关键词：人类运动矩阵运动学生物医学

Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface

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Food Science and Human Wellness 2024年第3期13卷 1556-1566页

作者： Pianpian Yan Ramachandran chelliah Kyoung Hee Jo Xiuqin chen Akanksha Tyagi Hyeon Yeong Jo Fazle Elahi Nam chan woo Min Seung wook Deog Hwan Oh Department of Food Science and Biotechnology College of Agriculture and Life SciencesKangwon National UniversityChuncheon 24341South Korea Kangwon Institute ofInclusive Technology(KIIT) Kangwon National UniversityChuncheon 24341South Korea Saveetha School of Engineering (SIMATS)UniversitySriperumbudur 600124India Seoulin Bioscience company Seongnam-si 13488South Korea

Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture *** study assessed the efficacy of SAEW(30 mg/l)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)*** underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was *** results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(cFU/g))reduction in pork,chicken,spinach,and *** heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(cFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell *** metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and ***,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃*** per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.

关键词： Multi-resistant Staphylococcus aureus Metabolic profile SAEW Biofilm Hurdle technology Electrode material

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites

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cancer communications 2024年第1期44卷 47-75页

作者： Ji Eon Kim So-Young Park chulhwan Kwak Yoonji lee Dae-Geun Song Jae woo Jung Haesong lee Eun-Ae Shin Yangie Pinanga Kyung-hee Pyo Eun Hae lee Wonsik Kim Soyeon Kim chang-Duck Jun Jeanho Yun Sun choi Hyun-woo Rhee Kwang-Hyeon liu Jung Weon lee Department of Pharmacy College of PharmacySeoul National UniversitySeoulRepublic of Korea Research Institute of Pharmaceutical Sciences College of PharmacySeoul National UniversitySeoulRepublic of Korea BK21 FOUR community-Based Intelligent Novel Drug Discovery Education Unit College of Pharmacy and Research Institute of Pharmaceutical SciencesKyungpook National UniversityDaeguRepublic of Korea Department of chemistry Seoul National UniversitySeoulRepublic of Korea college of Pharmacy Chung-Ang UniversitySeoulRepublic of Korea Natural Product Informatics Research center Korea Institute of Science and Technology(KIST)Gangneung-siGangwon-doRepublic of Korea School of life Sciences Gwangju Institute of Science and Technology(GIST)GwangjuRepublic of Korea Department of Biochemistry College of MedicineDong-A UniversityBusanRepublic of Korea Global AI Drug Discovery center College of Pharmacy and Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoulRepublic of Korea Interdisciplinary Program in Genetic Engineering Seoul National UniversitySeoulRepublic of Korea

Background:Transmembrane 4 l six family member 5(TM4SF5)translocates subcellularly and functions metabolically,although it is unclear how intracellu-lar TM4SF5 translocation is linked to metabolic *** is thus of interests to understand how the traffic dynamics of TM4SF5 to subcellular endosomal membranes are correlated to regulatory roles of ***:Here,we explored the metabolic significance of TM4SF5 localization at mitochondria-lysosome contact sites(MlcSs),using in vitro cells and in vivo animal systems,via approaches by immunofluorescence,proximity labelling based proteomics analysis,organelle reconstitution ***:Upon extracellular glucose repletion following depletion,TM4SF5 became enriched at MlcSs via an interaction between mitochondrial FK506-binding protein 8(FKBP8)and lysosomal *** labeling showed molecular clustering of phospho-dynamic-related protein I(DRP1)and certain mitophagy receptors at TM4SF5-enriched MlcSs,leading to mitochondrial fis-sion and ***4SF5 bound NPc intracellular cholesterol transporter 1(NPc1)and free cholesterol,and mediated export of lysosomal cholesterol to mitochondria,leading to impaired oxidative phosphorylation but intact tri-carboxylic acid(TcA)cycle andβ-*** mouse models,hepatocyte Tm4sf5 promoted mitophagy and cholesterol transport to mitochondria,both with positive relations to liver ***:Our findings suggested that TM4SF5-enriched MlcSs regu-late glucose catabolism by facilitating cholesterol export for mitochondrial reprogramming,presumably while hepatocellular carcinogenesis,recapitulating aspects for hepatocellular carcinoma metabolism with mitochondrial repro-gramming to support biomolecule synthesis in addition to glycolytic energetics.

关键词： cholesterol fluorescent imaging glucose catabolism hepatocellular carcinogenesis mem-brane contact sites mitochondria function mitophagy oxidative phosphorylation protein-protein interaction tetraspanin

Efficacy of chemotherapy containing bevacizumab in patients with metastatic colorectal cancer according to programmed cell death ligand 1

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3521-3528页

作者： Shin woo Kang Sung Hee lim Min-Ji Kim Jeeyun lee Young Suk Park Ho Yeong lim Won Ki Kang Seung Tae Kim Division of Hematology Oncology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351South Korea Samsung Biomedical Research Institute Samsung Medical CenterSeoul 06351South Korea

BAcKGROUND Bevacizumab,an anti-vascular endothelial growth factor(VEGF)monoclonal antibody,inhibits angiogenesis and reduces tumor *** VEGF-c,lactate dehydrogenase,and inflammatory markers have been reported as predictive markers related to bevacizumab *** cell death ligand 1(PD-l1)could act upon VEGF receptor 2 to induce cancer cell angiogenesis and *** To investigate the efficacy of bevacizumab-containing chemotherapy in patients with metastatic colorectal cancer(cRc)according to the expression of *** This analysis included cRc patients who received bevacizumab plus FOlFOX or FOlFIRI as first-line therapy between June 24,2014 and February 28,2022,at Samsung Medical center(Seoul,South Korea).Analysis of patient data included evaluation of PD-l1 expression by the combined positive score(cPS).We analyzed the efficacy of bevacizumab according to PD-l1 expression status in patients with *** A total of 124 patients was included in this *** all patients were treated with bevacizumab plus FOlFIRI or FOlFOX as the first-line *** 77%of patients received FOlFOX,23%received FOlFIRI as backbone first-line *** numbers of patients with a PD-l1 cPS of 1 or more,5 or more,or 10 or more were 105(85%),64(52%),and 32(26%),*** results showed no significant difference in progression-free survival(PFS)and overall survival(OS)with bevacizumab treatment between patients with PDl1 cPS less than 1 and those with PD-l1 cPS of 1 or more(PD-l1l1≥1%;PFS:P=0.93,OS:P=0.33),between patients with PD-l1 cPS less than 5 and of 5 or more(PD-l1l1≥5%;PFS:P=0.409,OS:P=0.746),and between patients with PD-l1 cPS less than 10 and of 10 or more(PD-l1l1≥10%;PFS:P=0.529,OS:P=0.568).cONclUSION chemotherapy containing bevacizumab can be considered as first-line therapy in metastatic cRc irrespective of PD-l1 expression.

关键词： Bevacizumab colorectal cancer Programmed cell death ligand 1 expression First-line chemotherapy Metastatic colorectal cancer

Professor Yuan cheng (Bert) Fung:My Respected Mentor and cherished Friend

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医用生物力学 2018年第S1期33卷 7-15页

作者：胡流源 Department of Bioengineering Swanson School of EngineeringUniversity of Pittsburgh

On September 14,2008,*** chien,Peter chen,Geert Schmid-Schonbein,Pin Tong and I had the great pleasure of organizing a beautiful event in San Diego,california to celebration ***’s 90thbirthday with his students,friends and family[1].The team gave me the honor and privilege to be the banquet speaker.I titled my speech as“***:My Respected Mentor and cherished Friend”-something for those of you who have known *** well can“resonate”.Still,I would like to take this opportunity to share with you an excerpt of the story I told about this great gentle man.

关键词： Fung:My Respected Mentor and cherished Friend Professor Yuan cheng

A STUDY ON cHINESE PHENYlAlANINE HYDROXYlASE GENE RESTRIcTION SITE POlYMORPHISM

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Science china chemistry 1988年第12期31卷 1447-1453页

作者：曾溢滔,黄淑帧,陈美珏,张美兰,任兆瑞,徐永华,彭素芬,savio l. c. woo,胡流清 laboratory of Medical Genetics Shanghai children's Hospital laboratory of Medical Genetics Shanghai children's Hospital laboratory of Medical Genetics Shanghai children's Hospital laboratory of Medical Genetics Shanghai children's Hospital laboratory of Medical Genetics Shanghai children's Hospital Shanghai Institute of cell Biology Academia Sinica Shanghai Institute of cell Biology Academia Sinica Department of cell Biology Baylor college of Medicine Houston Texas 77030 U.S.A. Department of cell Biology Baylor college of Medicine Houston Texas 77030 U.S.A.

Human phenylalanine hydroxylase (PAH) cDNA was applied as a hybridization probe to analyzing the following 8 restriction fragment length polymorphisms (RFlP) in the PAH genes of 80 normal and 28 phenylketonuric chinese patients: BgⅢ, 3.6kb/1.7kb; EcoRI, 17kb/11kb; EcoRV, 30kb/25kb; HindⅢ, 4.2kb/4.0kb; MspIa, 23kb/19kb; MspIb, 4.0kb/2.2kb; PvuIIa, 19kb/6.0kb and PvuIIb, 11.5kb/9.1kb. The frequencies of the above RFlP in normal chinese and PKU patients are: 0.13, 0.83, 0.77, 0.81, 0.12, 0.04, 0.70, 0.10 and 0.12, 0.93, 0.89, 0.81, 0.04, 0, 0.69, 0.04, respectively. This study reveals significant differences between the frequencies of individual RFlPs when these values are compared with those of the caucasians. Finally, the detection of RFlPs in the PAH gene in the chinese population will permit prenatal diagnosis of PKU in china.

关键词： phenylketonuria chinese PAH gene polymorphism.