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用酶联免疫吸附试验检验肌旋毛虫

肉品卫生 1994年第12期 29-31页

作者： kitamura 付刚

目前,在日本虽然旋毛虫发病率很低,俱食品卫生部门对该病的危害切不可忽视。近年来日本的猪肉进口量正在逐年增加,而输出猪肉国的旋毛虫病比昔日(1952年至1954年,Kagei,N.)更加流行。在日本,食用生火腿的人数也趋于增加。传统的肌旋毛... 详细信息

目前,在日本虽然旋毛虫发病率很低,俱食品卫生部门对该病的危害切不可忽视。近年来日本的猪肉进口量正在逐年增加,而输出猪肉国的旋毛虫病比昔日(1952年至1954年,Kagei,N.)更加流行。在日本,食用生火腿的人数也趋于增加。传统的肌旋毛虫检验法都会有漏检,

关键词：食品检验肌旋毛虫酶联免疫法肉制品

Comparative studies of serum-free media and detection techniques for in vitrodrug sensitivity assessment of Plasmodium falciparum

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Open Journal of Clinical Diagnostics 2013年第3期3卷 115-121页

作者： Bethel Kwansa-Bentum Shinji Izumiyama Kei kitamura Kazushige Obata-Ninomiya Nobuo Ohta Hiroko Asahi Department of Animal Biology and Conservation Science Faculty of Science University of Ghana Accra Ghana Department of Environmental Parasitology Tokyo Medical and Dental University Tokyo Japan Department of Immune Regulation Tokyo Medical and Dental University Tokyo Japan Department of Parasitology National Institute of Infectious Diseases Tokyo Japan

Malaria continues to be a devastating disease. In a previous study, we formulated a chemically defined culture medium that is able to sustain the complete intraerythrocytic growth of Plasmodium falciparum. We tested the feasibility of using the medium (CDRPMI) as well as human serum-free media enriched with commercially available human-serum substitutes (GFSRPMI and ALBRPMI) to assess the drug sensitivity of P. falciparum, using chloroquine diphosphate (CQ) and dihydroartemisinin (DHART) as conventional antimalarial drugs. Growth inhibition was measured by four different methods: flow cytometry with SYBR Green I (FCM), microscopy (Giemsa method), enzymatic estimation of parasite lactate dehydrogenase (pLDH), and histidine-rich protein 2 (HRPII) determination. In drug sensitivity tests on asynchronous parasites cultured for 96 h in the presence of drugs, the dose-response curves were similar and differences in the 50% growth inhibition concentrations for the drugs, which were estimated by the four methods, were not statistically significant for the three culture media. The effect of the drugs on the growth of synchronous parasites at the ring stage was also assessed in micro-volume tests by three different methods of FCM: tracking fluorescent erythrocytes, schizont test, and merozoite test. Dose-response curves for the drugs were similar, and differences in the 50% growth inhibition concentrations were not statistically significant for CDRPMI and GFSRPMI. Thus CDRPMI as well as GFSRPMI and ALBRPMI can be similarly useful media for drug sensitivity testing of P. falciparum. The FCM, pLDH and HRPII estimations were fast and reliable detection methods, with FCM allowing schizont and merozoite tests to be performed with shorter periods of culture.

关键词： Plasmodium falciparum Chemically Defined Medium Drug Sensitivity Test Flow Cytometry

Role of Mash Ⅰ in Lung Growth and Differentiation using Mash Ⅰ Defictent Mice

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Role of Mash Ⅰ in Lung Growth and Differentiation using Mas...

Microhabitat Use and Population Structure of a Chinese Kissing Loach, Leptobotia tchangi, in the North Tiaoxi River, China

ThirdCongressoftheAsian...

作者： Naoko UDAKA Takaaki ITO Takayuki IKOMA Hitoshi kitamura Ryoichiro KAGEYAMA Pathol. Division Kanagawa Cardiovascular Respiratory Center6-16-1 TomiokahigashiKana/awa-kuYokohama Dept of Pathol. Yokohama City Univ Sch of Medicine3-9 FukuuraKanazawa-kuYokohama Dept of Pathol. Yokohama City Univ Sch of Medicine3-9 FukuuraKanazawa-kuYokohama Dept of Pathol. Yokohama City Univ Sch of Medicine3-9 FukuuraKanazawa-kuYokohama Institute for Virus Research.kyoto Univ. 53 Shougoin KawaramachiSakyou-kuKyotoJAPAN

＜正＞In vertebrates,a basic helix-loop-helix transcription factor that is homologous to the Drosophila achaete-scute complex plays a critical role in the development and differentiation in neural cells and neuroendocrine cells. In the lung, pulumonary neuroendoerine cells（PNEC） are distributed throughout the intraplumonary airway Trom the large bronehi to the alveolar duets, which occur

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Open Journal of Ecology 2014年第7期4卷 337-345页

作者： Liangliang Huang Jianhua Li Yuichi Kano Tatsuro Sato Jyunichi kitamura Yukihiro Shimatani Zhiqiang Wu Department of Urban and Environmental Engineering Graduate School of Engineering Kyushu University Fukuoka Japan Guangxi Scientific Experiment Center of Mining Metallurgy and Environment Guilin University of Technology Guilin China Key Laboratory of Yangtze River Water Environment Ministry of Education Tongji University Shanghai China Mie Prefectural Museum Mie Japan

Fundamental ecological information on the Chinese kissing loach (Leptobotia tchangi) was collected in the North Tiaoxi River, China, in September 2010. The length-frequency distribution calculated using FiSAT software showed the population structure of this kissing loach could be classified into four age groups suggesting that the life span of the loach should be 3 - 4 years. A stepwise multiple linear regression analysis revealed that the density of this kissing loach was significantly and positively correlated with water velocity, while the body size was significantly and positively correlated with river bed pebble size. It suggested that water velocity and variation of pebble size play important roles in the full life history of this kissing loach. Thus, maintenance of the rapid current and variety of substrate are essential for the conservation of this species under the present conditions in the river, which also can support river restoration being in harmony with survival of this kissing loach.

关键词： Biodiversity Conservation Fish Population Riffle-Pool Structure Microhabitat

Suppression of Sup35 amyloid fibril formation by group II chaperonin from Thermoplasma acidophilum

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American Journal of Molecular Biology 2012年第3期2卷 265-275页

作者： Kentaro Noi Aya kitamura Hidenori Hirai Kunihiro Hongo Toshihiko Sakurai Tomohiro Mizobata Yasushi Kawata Department of Biomedical Science Institute of Regenerative Medicine and Biofunction Graduate School of Medical Science Department of Chemistry and Biotechnology Graduate School of Engineering Tottori University Tottori Japan

The Group II chaperonin from Thermoplasma acidophilum was added to the in vitro amyloid fibrillation reaction of yeast Sup35NM protein to assess its effects. By measuring the formation of Sup35NM fibrils in real time using the fluorescent dye Thioflavin T, we found that the addition of T. acidophilum-cpn α16, α1, and β1 proteins suppressed fibril formation. Addition of a 0.1 molar-equivalent T. acidophilum-cpn α16 relative to Sup35NM prolonged the initial lag-time of fibril formation and decreased the rate of fibril extension. Addition of 1 or 3 molar-equivalents of T. acidophilum-cpn monomers also produced a similar effect. Delayed addition of these chaperonins after the initial lag phase did not suppress fibril formation. Interestingly, these effects were also observed upon adding only the apical domain segments of α and β-subunits, and we also found that deletion of the helical protrusion in the apical domain of these segments led to an abolishment of the suppression effects. A synthetic peptide whose sequence corresponded to the helical protrusion also displayed a suppression effect, which indicated that archaeal group II chaperonin binds to Sup35NM through the helical protrusion of the apical domain. These findings suggest that group II chaperonin might be actively involved in suppressing amyloid fibril formation, in addition to acting as a protein folding assistant.

关键词： Group II Chaperonin Monomer Thermoplasma Acidophilum Structure and Function Suppression of Amyloid Fibril Sup35NM Amyloid

Clinicopathological Analysis of Patients with Nonmuscle-Invasive Bladder Cancer Who Underwent Radical Cystectomy

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Surgical Science 2012年第7期3卷 347-352页

作者： Naotaka Nishiyama Hiroshi kitamura Masatoshi Mutoh Masahiro Yanase Hiroyuki Iwaki Tadashi Hasegawa Taiji Tsukamoto Departments of Surgical Pathology Sapporo Medical University School of Medicine Sapporo Japan Departments of Urology Sapporo Medical University School of Medicine Sapporo Japan Divisions of Pathology Sunagawa City Medical Center Sunagawa Japan Divisions of Urology Sunagawa City Medical Center Sunagawa Japan

Objectives: Surgical specimens obtained at the time of the last transurethral resection of bladder tumor (TURBT) for patients with nonmuscle-invasive bladder cancer (NMIBC) who underwent radical cystectomy were retrospectively evaluated in order to investigate the relationship between pathological variation and upstaging of NMIBC. Methods and Materials: Twenty patients (19 men, 1 woman;aged 69.4 ± 12.1 (mean ± SD) years) diagnosed with NMIBC underwent radical cystectomy during follow-up. Results: Five of the 20 patients (25%) had pathological upstaging in the radical cystectomy specimens. There was a statistical association between pathological upstaging and cancer death (p = 0.002). There were three patterns of pathological variation in the upstaged specimens: 1) in patients with BCG-resistant NMIBC, urothelial carcinoma invaded through the lamina propria;2) urothelial carcinoma showed diffuse invasion beyond the deep lamina propria, and the cancer cells had infiltrated as single cells and formed nodules;3) TURBT specimens showed a micropapillary variant. Conclusions: Since these pathological variations correlated with pathological upstaging, they may provide an indication for cystectomy in NMIBC patients.

关键词： Clinicopathological Examination Nonmuscle-Invasive Bladder Cancer Radical Cystectomy Pathological Upstaging

Extraction of Phosphorous from Steelmaking Slag by Leaching

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Extraction of Phosphorous from Steelmaking Slag by Leaching

“第十届中国钢铁年会”暨“第六届宝钢学术年会”

作者： Kim Sun-Joong Gao Xu Uedaand Shigeru kitamura Shin-ya Institute of Multidisciplinary Research for Advanced Materials Tohoku University

In order to recyclephosphorusfrom steelmaking slag, a separation processby leaching has been proposed by current *** principle of this leaching process was to utilize the differencesin the water-solubility betweenthe CS-C Psolid solution and the other phases. Experimentswere carried out to determinethe effect of pH on the dissolution behavior of various elements in the steelmaking slagwith and without free-CaO *** the pH was decreased, the dissolution behavior of Ca, Si, P and Fe from the steelmaking slag was increased. Furthermore, at a pH of 3, the dissolution ratio of P from steelmaking slag without the free-CaO was larger than that from steelmaking slag including free-CaO.

关键词： steelmaking slag C2S-C3P Presources leaching process

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机化肺炎的临床研究

中华结核和呼吸杂志 1997年第Z1期20卷 84-84页

作者： Tatsuno M Kobayashi J kitamura S 曾小明(译) DepartmentofPulmonaryMedicine JichiMedicalSchoolTochigiJapan

壅塞机制对RBCC引射模态推力增强性能的影响

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壅塞机制对RBCC引射模态推力增强性能的影响