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Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa

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Virologica Sinica 2013年第5期28卷 266-271页

作者： Tong Ou sanhua li Xiangyong liao Qiya Zhang State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences

The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,***-one cyanobacterial strains were used to detect the host range of *** aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further *** microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in *** one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80infectious units per cell,*** endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 *** dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis revealed four major structural *** results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.

关键词： Cyanophages Myovirus Microcystis aeruginosa Bloom-forming cyanobacterium Genomic DNA Major structuralproteins

Two Virus-like Particles that Cause Lytic Infections in Freshwater Cyanobacteria

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Virologica Sinica 2013年第5期28卷 303-305页

作者： sanhua li Tong Ou Qiya Zhang State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences

Dear Editor,We report the results of the preliminary isolation of two virus-like particles(VLPs)that are infectious to freshwater cyanobacteria from Lake Donghu,the largest urban lake in China,located in Wuhan City,Hu... 详细信息

关键词：病毒样颗粒水华蓝藻淡水感染裂解透射电子显微镜铜绿微囊藻城市湖泊

Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species

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BIOCELL 2021年第4期45卷 943-951页

作者： CHUNJIAO liU QINHONG KONG FENG PAN SHAN JIANG liNGJIE MENG GAI HUANG liDAN LU sanhua li YUN liU Guizhou Provincial College-based Key Laboratory for Tumor Prevention and Treatment with DistinctiveMedicines ZunyiMedical UniversityZunyi563000China life Sciences Institute Zunyi Medical UniversityZunyi563000China Department of Biochemistry and Molecular Biology Zunyi Medical UniversityZunyi563000China

Traditional Chinese medicine(TCM)has been increasingly applied in both preventing and treating a variety of cancers in the last decades,attributing to its fewer side effects as compared with chemotherapy ***,a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various ***,the effect and mechanism of hellebrigenin on colorectal cancers were still ***,the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in vitro and in ***,hellebrigenin led to a reduction of mitochondrial membrane *** addition,treatment with hellebrigenin could result in the induction of excessive reactive oxygen species,which led to cell *** results indicated that hellebrigenin had anti-cancer potential in the treatment of colorectal cancers.

关键词： Hellebrigenin Colorectal cancer Reactive oxygen species Apoptosis

3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway

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BIOCELL 2022年第11期46卷 2425-2432页

作者： sanhua li QINGHONG KONG XIAOKE ZHANG XINTING ZHU CHUNBO YU CHANGYAN YU NIAN JIANG JING HUI liNGJIE MENG YUN liU Guizhou Provincial College–Based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines Zunyi563000China life Science Institute of Zunyi Medical University Zunyi563000China School of Preclinical Medicine of Zunyi Medical University Zunyi563000China

Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor ***,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well *** present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in ***,3-epi-bufotalin inhibited the JAK1/STAT3 signaling *** results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells.

关键词： 3-epi-bufotalin Colorectal cancer JAK1/STAT3 signaling pathway Apoptosis

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与分担全纯函数相关的正规族

数学年刊（A辑） 2016年第1期37卷 89-96页

作者：李三华井冈山大学数理学院江西吉安343009

设F是在区域D内的一族亚纯函数,其零点重级至少为k,k是一个正整数,a(z)(≠0)在区域D内全纯.若对于任意的f∈F,有(1)f(z)与a(z)没有公共的零点;(2)f(z)=0f(k)(z)=a(z)■0<|f^((k+1))(z)-a'(x)|<|a(z)|,则F在D内正规.

关键词：亚纯函数零点正规族

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一个有关素数幂的三元丢番图不等式

数学进展 2022年第3期51卷 447-470页

作者：李三华上海立信会计金融学院统计与数学学院上海201620

令1

关键词：丢番图不等式指数和素数

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有关殆素数的二元丢番图不等式

数学年刊（A辑） 2021年第2期42卷 179-188页

作者：李三华吴高翔井冈山大学数理学院江西吉安343009

Pr表示最多r个素因子的正整数.作者证明了,对于任一足够大的实数N和1

关键词：丢番图不等式指数和殆素数

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超高强弹性铜合金材料的研究进展与展望

材料导报 2015年第7期29卷 1-5页

作者：李周雷前黎三华申镭诺李思中南大学材料科学与工程学院长沙410083 有色金属材料科学与工程教育部重点实验室长沙410083

超高强弹性铜合金是导电弹性元器件的关键材料之一,它广泛应用在电子电工、通信导航、汽车工业和海洋工程等领域。综述了超高强铜合金材料的研究开发现状,以强度超过1000 MPa的Cu-Be、Cu-Ti、Cu-Ni-Sn、CuNi-Mn、Cu-Ni-Si和Cu-Ni-Al合... 详细信息

超高强弹性铜合金是导电弹性元器件的关键材料之一,它广泛应用在电子电工、通信导航、汽车工业和海洋工程等领域。综述了超高强铜合金材料的研究开发现状,以强度超过1000 MPa的Cu-Be、Cu-Ti、Cu-Ni-Sn、CuNi-Mn、Cu-Ni-Si和Cu-Ni-Al合金为例,概述了它们的合金化设计原则,时效过程中的相变机理,包括相变贯序、析出相的分布、取向关系等,同时对它们的制备特点、主要应用领域、强化机制和相关物理性能进行了阐述,在此基础上对它们开发应用中所存在的问题、前景和市场需求进行了分析和展望。

关键词：超高强铜合金弹性材料

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钯催化卤代芳烃的胺化反应研究

化学进展 2005年第2期17卷 286-292页

作者：刘蒲李三华李利民王向宇殷元骐张玉华郑州大学化学系郑州450052 中国科学院兰州化学物理研究所兰州730000

钯催化卤代芳烃胺化是形成CAr—N的重要方法。配体的发展扩展了底物的适用范围 ,提高了反应的选择性 ,实现了廉价易得的氯代芳烃的胺化 ,弱碱的使用提高了官能团的兼容性 ,因此Pd催化芳胺化广泛应用于合成芳胺类化合物。本文以卤代芳烃... 详细信息

钯催化卤代芳烃胺化是形成CAr—N的重要方法。配体的发展扩展了底物的适用范围 ,提高了反应的选择性 ,实现了廉价易得的氯代芳烃的胺化 ,弱碱的使用提高了官能团的兼容性 ,因此Pd催化芳胺化广泛应用于合成芳胺类化合物。本文以卤代芳烃为线索 ,对钯催化偶联胺化反应的研究进展进行了综述和展望。

关键词：卤代芳烃钯催化胺化偶联