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Cytotoxic effect of Spirulina platensis extracts on human acute leukemia Kasumi-1 and chronic myelogenous leukemia K-562 cell lines

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Asian Pacific Journal of Tropical Biomedicine 2017年第1期7卷 14-19页

作者： Flor Yohana Flores Hernandez sanghamitra khandual Inocencia Guadalupe Ramírez López Center for Investigation and Assistance in Technology Design for State of JaliscoC.P.45019ZapopanJaliscoMexico

Objective: To evaluate the cytotoxic effects of Spirulina platensis extracts on acute leukemia Kasumi-1 and chronic leukemia K-562 cancer cell ***: Various concentrations of Spirulina platensis extracts(0.25–50.00 mg/m L)obtained with different solvents were used to treat cell lines for 72 h. For cytotoxic effect studies, cell viability test with trypan blue solution, MTT assay and microscopic cytomorphological assessment were ***: Spirulina extract obtained with 70% ethanol showed significant cytotoxicity in K562 and Kasumi-1 cell lines. With trypan blue solution, IC_(50) values were found to be4.64 mg/m L for K-562 and 3.68 mg/m L for Kusumi-1 cell lines. Spirulina aqueous extract also showed cytotoxicity with trypan blue method, at a slightly higher dose; where IC_(50) values were 12.68 mg/m L for K-562 and 2.13 mg/m L for Kusumi-1 cell lines. The IC_(50) values were found 0.40 mg/m L for K-562 and 0.31 mg/m L for Kusumi-1 cell lines for the 70% ethanol extract according to the MTT assay. Spirulina extract obtained with water also showed cytotoxicity but the dose was a little higher where IC_(50) values were15.77 mg/m L for K-562 and 9.44 mg/m L for Kusumi-1 cell lines. The effect of cytotoxicity with ethanol extract is quite comparable with that observed for cyclophosphamide, which is a chemical used as anticancer ***: The cytotoxicity exhibited by Spirulina extract to cancer cell lines might be due to the presence of phytopigments(carotenoids, chlorophyll, phycocyanin) as well as polysaccharides that were reported previously as constituents of the extract. So crude extracts of Spirulina can be used as a source to develop anticancer drugs.

关键词： Leukemia Cytotoxicity Spirulina Carotenoids

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Rapid,Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris

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Advances in Bioscience and Biotechnology 2014年第4期5卷 333-339页

作者： sanghamitra khandual Pallavolu Maheswara Reddy Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco(CIATEJ) GuadalajaraMéxico The Energy and Resources Institute New DelhiIndia

A rapid, efficient and high-performance transformation protocol employing Agrobacterium rhizogenes was developed for the common bean Phaseolus vulgaris. In this study, we examined competencies of various protocols to induce and explants that respond to hairy root transformation in bean plants. Utilizing young seedlings with severed radicles/hypocotyls, we developed a highly efficient procedure for achieving hairy root transformation frequencies as high as 100% as visualized by GUS reporter gene expression system. Transgenic hairy roots in these young composite plants were susceptible to nodulation by rhizobia, and form an excellent system for high throughput genomic analysis to study root biology and endosymbiosis in common bean.

关键词： Phaseolus vulgaris Agrobacterium rhizogenes Hairy Root Transformation GUS Expression

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Comparative Yeild and Oil Quality of Toxic and Non-Toxic Mexican Jatropha curcas Grown in the Same Agroclimatic Conditions

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American Journal of Plant Sciences 2014年第2期5卷 230-234页

作者： khandual sanghamitra Ramirez Victorin Oramas Rout Nutan Prasad Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.Av.Normalistas#800Colinas de la NormalGuadalajaraMexico Colegio de Postgraduados-Campeche Campus CampecheMexico

Jatropha curcas brings attention across the developing world for biodiesel production since it grows in tropical and subtropical climates with no other competing food uses. This crop is gaining popularity in Mexico for biofuel production. Currently, there are not many reports on the native varieties and their yield to estimate the feasibility of the particular genetic resource to use it as an economic crop. So in this part of work an agronomical evaluation of yield, oil content, fatty acid types and biodiesel conversion from the two native varieties of Jatropha curcas from Mexico were compared while being produced in the same agro-climatic condition. The varieties used were based on the phorbol ester content like toxic and nontoxic varieties collected from Yucatan and Veracruz. It was found that the oil content in the seeds of toxic variety was 57% and oil content of the nontoxic variety was 54% and also the seed yield is higher. The fatty acid content also varies among the two varieties like 21% and 23% in toxic and nontoxic varieties respectively. Again in the nontoxic variety the content of monosaturated fatty acid was higher, which suggests that better quality biodiesel may be expected from this genotype. Transestrification of the two kinds of oil was done using KOH as a catalyst, but no significant difference was observed in the conversion of biodiesel among the two types of oil from the two genotypes.

关键词： Biodiesel Toxic Biofuel Transesterification

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Isolation,Identification and Germplasm Preservation of Different Native Spirulina Species from Western Mexico

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American Journal of Plant Sciences 2013年第12期4卷 65-71页

作者： Rout Nutan Prasad khandual sanghamitra Gutierrez-Mora Antonia Gallardo-Valdéz Juan Rodriguez-Garay Benjamin Ibarra-Montoya JLuis Vega-Valero Guillermo Centro de Investigación y Asistencia en Tecnología y Diseno del Estado de Jalisco A.C.Av.Normalistas#800Colinas de la NormalGuadalajaraJaliscoMexico Biotecnología Mexicana de Microalgas S.A.de C.V.Sta.Maria 136 Conlonia TepeyacZapopanJaliscoMexico

Spirulina is an edible algae and has a wide range of pharmaceutical applications in addition to its nutritional value. Isolation and identification of several Spirulina species were conducted in the western part of Mexico especially in the state of Jalisco. The purification strategy consisted of five optimized processing steps: 1) washing and centrifugation, 2) chemical treatment, 3) micromanipulation, 4) serial dilution, and 5) plating. Four species were isolated from different locations and two out of these four species were identified taxonomically up to the species level: Spirulina subsalsa and S. major. For short term conservation (30 days), the strains were maintained in liquid and solid agar medium at 10?C and 4?C. For medium term (few months), they were preserved in solid medium under a dried condition as agar flakes and for long term, cryopreservation was employed by using 5% and 10% DMSO, glycerol and methanol as osmoprotectants in liquid nitrogen. For short term preservation nearly 90% liquid and 100% agar recovered strains were viable after one month at both temperatures. In the case of the agar flakes, cells were viable after three months of conservation at room temperature. Cryopreservation did not give any suitable results after three months of conservation. Variable and two-step improved cryopreservation processes are now in progress for conservation.

关键词： Germplasm Cryopreservation Spirulina Long Term Preservation

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Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

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aBIOTECH 2024年第2期5卷 151-168页

作者： Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla ICAR National Rice Research Institute CuttackOdisha 753006India Department of Botany Ravenshaw UniversityCuttackOdisha 753003India Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA

The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic ***-predicted guide RNAs(gRNAs)often fail to induce efficient cleavage at target *** target loci are inaccessible due to complex chromatin ***,there is no suitable tool available to predict the architecture of genomic target sites and their ***,significant time and resources are spent on performing editing experiments with inefficient *** in vitro-cleavage assay could provide a rough assessment of gRNA efficiency,it largely excludes the interference of native genomic *** in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic ***,we developed a modified protocol that offers highly efficient protoplast isolation from rice,Arabidopsis,and chickpea,using a sucrose gradient,transfection using PEG(polyethylene glycol),and validation of single guide RNAs(sgRNAs)cleavage efficiency of *** have optimized various parameters for PEG-mediated protoplast transfection and achieved high transfection efficiency using our protocol in both monocots and *** introduced plasmid vectors containing Cas9 and sgRNAs targeting genes in rice,Arabidopsis,and chickpea *** dual sgRNAs,our CRISPRdeletion strategy offers straightforward detection of genome editing success by simple agarose gel *** sequencing of PCR products confirmed the editing efficiency of specific ***,we demonstrated that isolated protoplasts can be stored for up to 24/48 h with little loss of viability,allowing a pause between isolation and *** high-efficiency protocol for protoplast isolation and transfection enables rapid(less than 7 days)validation of sgRNA cleavage efficiency before proceeding with stable *** isolation and transfection method can als

关键词： Protoplast Genome editing Transgene-free edited plants Rice Chickpea

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A New Line Symmetry Distance and Its Application to Data Clustering

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Journal of Computer Science & Technology 2009年第3期24卷 544-556页

作者： Sriparna Saha sanghamitra Bandyopadhyay Machine Intelligence Unit Indian Statistical InstituteKolkataIndia

In this paper, at first a new line-symmetry-based distance is proposed. The properties of the proposed distance are then elaborately described. Kd-tree-based nearest neighbor search is used to reduce the complexity of computing the proposed line-symmetry-based distance. Thereafter an evolutionary clustering technique is developed that uses the new linesymmetry-based distance measure for assigning points to different clusters. Adaptive mutation and crossover probabilities are used to accelerate the proposed clustering technique. The proposed GA with line-symmetry-distance-based （GALSD） clustering technique is able to detect any type of clusters, irrespective of their geometrical shape and overlapping nature, as long as they possess the characteristics of line symmetry. GALSD is compared with the existing well-known K-means clustering algorithm and a newly developed genetic point-symmetry-distance-based clustering technique （GAPS） for three artificial and two real-life data sets. The efficacy of the proposed line-symmetry-based distance is then shown in recognizing human face from a given image.

关键词： unsupervised classification clustering symmetry property line-symmetry-based distance Kd-tree genetic algorithm face recognition

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First-principles prediction of electronic transport in fabricated semiconductor heterostructures via physics-aware machine learning

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npj Computational Materials 2021年第1期7卷 834-845页

作者： Artem K.Pimachev sanghamitra Neogi Ann and H.J.Smead Aerospace Engineering Sciences University of Colorado Boulder3775 Discovery DriveBoulder 80303 COUSA

First-principles techniques for electronic transport property prediction have seen rapid progress in recent ***,it remains a challenge to predict properties of heterostructures incorporating fabrication-dependent ***-learning(ML)approaches are increasingly being used to accelerate design and discovery of new materials with targeted properties,and extend the applicability of first-principles techniques to larger ***,few studies exploited ML techniques to characterize relationships between local atomic structures and global electronic transport *** this work,we propose an electronictransport-informatics(ETI)framework that trains on ab initio models of small systems and predicts thermopower of fabricated silicon/germanium heterostructures,matching measured *** demonstrate application of ML approaches to extract important physics that determines electronic transport in semiconductor heterostructures,and bridge the gap between ab initio accessible models and fabricated *** anticipate that ETI framework would have broad applicability to diverse materials classes.

关键词： coefficients principles electronic

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Separation of copper and iron from chloride media using Cyanex 921 in kerosene

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Journal of Central South University 2014年第5期21卷 1752-1755页

作者： sanghamitra Pradhan Niharbala Devi Sujata Mishra Department of Chemistry Institute of Technical Education and ResearchSiksha'O'Anusandhan University

The extraction and separation of Cu(Ⅱ)and Fe(Ⅲ)from HCl media using Cyanex 921 in kerosene were *** effect of shaking time,aqueous phase acid concentration,Fe(Ⅲ)/Cu(Ⅱ)concentration,Cyanex 921 concentration,temperature and aqueous to organic phase ratio on the separation of Cu(Ⅱ)and Fe(Ⅲ)was studied using 0.1 mol/L Cyanex 921 in *** separation was achieved from 2 mol/L HCl containing 0.001 mol/L Cu(Ⅱ)and 0.005 mol/L Fe(Ⅲ)with 0.1 mol/L Cyanex 921 in *** separation of copper and iron was also possible from 5 mol/L HCl using 0.02 mol/L Cyanex 921.

关键词： solvent extraction copper iron hydrochloric acid Cyanex 921

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Differential alterations of positive and negative regulators of beta catenin enhance endogenous expression and activity of beta catenin in A549 non small cell lung cancer(NSCLC)cells

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Genes & Diseases 2016年第4期3卷 282-288页

作者： Supratim Ghatak sanghamitra Raha Crystallography and Molecular Biology Division Saha Institute of Nuclear Physics1/AF BidhannagarKolkata 700064India Department of Biotechnology and ISERC Visva Bharati UniversitySantiniketan731235India Present address:Centre for Cellular and Molecular Biology Uppal RoadHyderabad 500007India

Beta catenin has been well documented in previous studies to be involved in non small cell lung cancer(NSCLC).Beta catenin abundance and transcriptional activity are significantly regulated by several *** it is well known that Akt and Gsk3 beta are respective positive and negative regulators of beta catenin,however,no single study has so far documented how the expression and activity of both positive as well as negative regulators play favorable role on beta catenin expression and activity in *** this study,we compared expression and activity of beta catenin and its regulators in normal lung cell WI38 and NSCLC cell A549 by western blot,qRT-PCR and luciferase *** observed that beta catenin positive regulators(Akt and Hsp90)and negative regulators(Gsk3 beta and microRNA-214)have differential expression and/or activity in NSCLC cell *** the differentially altered statuses of both the positive and negative regulators rendered cumulative positive effect on beta catenin expression and activity in *** study thus suggests that chemotherapeutic modulations of regulating factors are crucial when abrogation and/or inhibition of key oncogenic proteins are necessary for cancer chemotherapy.

关键词： A549 Beta catenin Gsk3 beta miR-214 Non small cell lung cancer

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miRT:A Database of Validated Transcription Start Sites of Human MicroRNAs

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Genomics, Proteomics & Bioinformatics 2012年第5期10卷 310-316页

作者： Malay Bhattacharyya Manali Das sanghamitra Bandyopadhyay Machine Intelligence Unit Indian Statistical Institute Department of Bioinformatics West Bengal University of Technology

MicroRNAs （miRNAs） are small endogenous non-coding RNAs of about 22 nt in length that take crucial roles in many biological pro cesses. These short RNAs regulate the expression of mRNAs by binding to their 3＇-UTRs or by translational repression. Many of the current studies focus on how mature miRNAs regulate mRNAs, however, very limited knowledge is available regarding their transcrip- tional loci. It is known that primary miRNAs （pri-miRs） are first transcribed from the DNA, followed by the formation of precursor miRNAs （pre-miRs） by endonuclease activity, which finally produces the mature miRNAs. Till date, many of the pre-miRs and mature miRNAs have been experimentally verified. But unfortunately, identification of the loci of pri-miRs, promoters and associated transcrip- tion start sites （TSSs） are still in progress. TSSs of only about 40% of the known mature miRNAs in human have been reported. This information, albeit limited, may be useful for further study of the regulation of miRNAs. In this paper, we provide a novel database of validated miRNA TSSs, miRT, by collecting data from several experimental studies that validate miRNA TSSs and are available for full download. We present miRT as a web server and it is also possible to convert the TSS loci between different genome built, miRT might be a valuable resource for advanced research on miRNA regulation, which is freely accessible at： http：//***/~bioinfo_miu/ miRT/***.

关键词： MicroRNA Transcription start site Primary transcript Transcriptional regulation Database

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