Rapid,Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris

作     者：Sanghamitra Khandual Pallavolu Maheswara Reddy 

作者机构：Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco(CIATEJ)GuadalajaraMéxico The Energy and Resources InstituteNew DelhiIndia 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2014年第5卷第4期

页      面：333-339页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Centro de Ciencias de Genomicas Universidad Autonoma de Mexico Cuernavaca Morelos Mexico for financial and lab support 

主　　题：Phaseolus vulgaris Agrobacterium rhizogenes Hairy Root Transformation GUS Expression 

摘      要：A rapid, efficient and high-performance transformation protocol employing Agrobacterium rhizogenes was developed for the common bean Phaseolus vulgaris. In this study, we examined competencies of various protocols to induce and explants that respond to hairy root transformation in bean plants. Utilizing young seedlings with severed radicles/hypocotyls, we developed a highly efficient procedure for achieving hairy root transformation frequencies as high as 100% as visualized by GUS reporter gene expression system. Transgenic hairy roots in these young composite plants were susceptible to nodulation by rhizobia, and form an excellent system for high throughput genomic analysis to study root biology and endosymbiosis in common bean.