检索结果-南通市图书馆

Changes of the liver metabolome following an intravenous lipopolysaccharide injection in Holstein cows supplemented with dietary carnitine

引用

Journal of Animal Science and Biotechnology 2023年第1期14卷 188-199页

作者： Wei Xu sandra Grindler Akos Kenez Sven Danicke Jana Frahm Korinna Huber Beijing Research Center of Intelligent Equipment for Agriculture Beijing 100097China. Department of Biosystems Biosystems Technology ClusterCampus GeelKleinhoefstraat 42440 Geel LeuvenKUBelgium. Institute of Animal Science Faculty of Agricultural SciencesUniversity of Hohenheim70599 StuttgartGermany. Department of Infectious Diseases and Public Health City University of Hong KongBlock 14/FTo Yuen Building31 To Yuen StreetKowloonHong Kong SARChina. Institute of Animal Nutrition Federal Research Institute for Animal Health(Friedrich-Loeffler-Institut)38116 BrunswickGermany.

Background:Carnitine facilitates the flux of long-chain fatty acids for hepatic mitochondrial beta-oxidation,which acts to ameliorate the negative energy balance commonly affecting high-yielding dairy *** triggered by lipopolysaccharide(LPS)load can however pose a challenge to the metabolic integrity via the expression of pro-inflammatory mediators,leading to immune system activation and respective metabolic *** effect of enhanced carnitine availability on hepatic metabolome profiles during an inflammatory challenge has not yet been determined in dairy ***,Holstein cows were supplemented with 25 g/d rumen-protected carnitine from 42d prepartum until 126 d postpartum(n=16)or assigned to the control group with no supplementation during the same period(n=14).We biopsied the liver of the cows before(100 d postpartum)and after(112 d postpartum)an intravenous injection of 0.5μg/kg *** samples were subjected to a targeted metabolomics analysis using the AbsoluteIDQ p180 Kit(Biocrates Life Sciences AG,Innsbruck,Austria).Results:Multivariate statistical analyses revealed that hepatic metabolome profiles changed in relation to both the carnitine supplementation and the LPS *** the metabolite profiles on 100 d,carnitine increased the concentration of short-and long-chain acyl-carnitines,which may be explained by an enhanced mitochondrial fatty acid shuttle and hence greater energy *** LPS injection affected hepatic metabolite profiles only in the carnitine supplemented group,particularly altering the concentration of biogenic ***:Our results point to interactions between an acute hepatic inflammatory response and biogenic amine metabolism,depending on energy availability.

关键词： Acyl-carnitines Inflammatory response Lipid metabolism Liver metabolome Mitochondrial function

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Emergence of a new satellite RNA from cucumber mosaic virus isolate P1

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2003年第3期4卷 97-100页

作者： sandra Pérez Alvarez,薛朝阳,周雪平 Institute of Biotechnology Zhejiang University Institute of Biotechnology Zhejiang University Institute of Biotechnology Zhejiang University Hangzhou 310029 China Hangzhou 310029 China Hangzhou 310029 China

The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat P1 1 and Sat P1 2). Sat P1 1 contained 335 nucleotides, and Sat P1 2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat P1 1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat P1 2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.

关键词： Cucumber mosaic virus Satellite RNA Sequence

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

In vivo evaluation of riboflavin receptor targeted fluorescent USPIO in mice with prostate cancer xenografts

引用

Nano Research 2016年第5期9卷 1319-1333页

作者： Jabadurai Jayapaul Susanne Ares Matt Bunker Marek Weiler sandra Rutherford Peter Comba Fabian Kiessling Institute for Experimental Molecular Imaging RWTH Aachen University Pauwelsstrasse 30 Aachen 52074 Germany Anorganisch-Chemisches Institut Universit#t Heidelberg Im Neuenheimer Feld 270 Heidelberg 59120 Germany Molecular Imaging Group Department of Structural Biology Leibniz Institut fur Molekulare Pharmakologie (FMP) Robert-Rossle-Str. 10 Berlin 13125 Germany AstraZeneca Pharmaceutical Development Charter Way Macclesfield Cheshire SK10 2NA UK Molecular Profiles Ltd. 8 Orchard Place Nottingham Business Park Nottingham NG8 6PX UK

Riboflavin （Rf） receptors bind and translocate Rf and its phosphorylated forms （e.g. flavin mononucleotide, FMN） into cells where they mediate various cellular metabolic pathways. Previously, we showed that FMN-coated ultrasmall superparamagnetic iron oxide （FLUSPIO） nanoparticles are suitable for labeling metabolically active cancer and endothelial cells in vitro. In this study, we focused on the in vivo application of FLUSPIO using prostate cancer xenografts. Size, charge, and chemical composition of FLUSPIO were evaluated. We explored the in vitro specificity of FLUSPIO for its cellular receptors using magnetic resonance imaging （MRI） and Prussian blue staining. Competitive binding experiments were performed in vivo by injecting free FMN in excess. Bio-distribution of FLUSPIO was determined by estimating iron content in organs and tumors using a colorimetric assay. AFM analysis and zeta potential measurements revealed a particulate morphology approximately 20-40 nm in size and a negative zeta potential （-24.23±0.15 mV） in water. X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry data confirmed FMN present on the USPIO nanoparticle surface. FLUSPIO uptake in prostate cancer cells and human umbilical vein endothelial cells was significantly higher than that of control USPIO, while addition of excess of free FMN reduced accumulation. Similarly, in vivo MRI and histology showed specific FLUSPIO uptake by prostate cancer cells, tumor endothelial cells, and tumor-associated macrophages. Besides prominent tumor accumulation, FLUSPIO accumulated in the liver, spleen, lung, and skin. Hence, our data strengthen our hypothesis that targeting riboflavin receptors is an efficient approach to accumulate nanomedicines in tumors opening perspectives for the development of diagnostic and therapeutic systems.

关键词： flavin mononucleotide iron oxide nanoparticles magnetic resonance imaging molecular imaging riboflavin carrier protein riboflavin transporters

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic testing vs microforceps biopsy in pancreatic cysts:Systematic review and meta-analysis

引用

World Journal of Gastroenterology 2019年第26期25卷 3450-3467页

作者： sandra Faias Luisa Pereira Angelo Luis Paula Chaves Marilia Cravo Department of Gastroenterology Instituto Portugues de Oncologia de Lisboa de Francisco GentilEPELisboa 1099-023Portugal Faculdade de Ciencias da Seude Universidade da Beira InteriorCovilha 200-506Portugal GRUBI-Grupo de Revisoes Sistematicas Universidade da Beira InteriorCovilha 6200-506Portugal Centro de Matemática e Aplicacoes(CMA-UBI) Universidade da Beira InteriorCovilha 6200-506Portugal Centro de Investigacao em Ciencias da Saude(CICS-UBI) Universidade da Beira InteriorCovilha 6200-506Portugal Department of Pathology Instituto Portugues de Oncologia de Lisboa de Francisco GentilEPELisboa 1099-023Portugal Department of Gastroenterology Hospital Beatriz AngeloLoures 2674-514Portugal Faculdade de Medicina Universidade de LisboaLisboa 1099-023Portugal

BACKGROUND Carcinoembryonic antigen(CEA)and cytology in pancreatic cystic fluid are suboptimal for evaluation of pancreatic cystic *** testing and microforceps biopsy are promising tools for pre-operative diagnostic improvement but comparative performance of both methods is *** To compare the accuracy of genetic testing and microforceps biopsy in pancreatic cysts referred for *** We performed a literature search in Medline,Scopus,and Web of Science for studies evaluating genetic testing of cystic fluid and microforceps biopsy of pancreatic cysts,with endoscopic ultrasound with fine-needle aspiration(EUSFNA)prior to surgery and surgical pathology as reference standard for *** evaluated the diagnostic accuracy for:1-benign cysts;2-mucinous low-risk cysts;3-high-risk cysts,and the diagnostic yield and rate of correctly identified cysts with microforceps biopsy and molecular *** also assessed publication bias,heterogeneity,and study *** Eight studies,including 1206 patients,of which 203(17%)referred for surgery who met the inclusion criteria were analyzed in the systematic review,and seven studies were included in the *** testing and microforceps biopsies were identical for diagnosis of benign *** analysis was superior for diagnosis of both low and high-risk mucinous cysts,with sensitivities of 0.89(95%CI:0.79-0.95)and 0.57(95%CI:0.42-0.71),specificities of 0.88(95%CI:0.75-0.95)and 0.88(95%CI:0.80-0.93)and AUC of 0.9555 and 0.92,*** diagnostic yield was higher in microforceps biopsies than in genetic analysis(0.73 vs 0.54,respectively)but the rates of correctly identified cysts were identical(0.73 with 95%CI:0.62-0.82 vs 0.71 with 95%CI:0.49-0.86,respectively).CONCLUSION Genetic testing and microforceps biopsies are useful second tests,with identical results in benign pancreatic *** analysis performs better for low-and high-risk cysts but has lower diagnos

关键词： Pancreatic cysts Endoscopic ultrasound Endoscopic ultrasound with fineneedle aspiration Genetic testing Microforceps biopsy Molecular analysis KRAS Carcinoembryonic antigen Cytology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of barium@alginate adsorbents for sulfate removal in lithium refining

引用

Frontiers of Chemical Science and Engineering 2021年第1期15卷 198-207页

作者： Lisa Xu Kaifei Chen George Q.Chen sandra E.Kentish Gang(Kevin)Li Department of Chemical Engineering The University of MelbourneParkvilleVIC 3010Australia

The demand for lithium has been steadily growing in recent years due to the boom of electric *** purity lithium is commonly used in the manufacture of battery grade lithium *** residuals originating from acid leaching of lithium ores must be limited to below 20 mg·L^(−1) during *** are methods to remove sulfate such as membrane processing and chemical precipitation using barium ***,membrane separation is unable to achieve the required purity while chemical precipitation often causes secondary contamination with barium and requires extra filtration processes that lead to increased processing *** this study,we developed a polymeric matrix entrapped with barium ions as a novel adsorbent to selectively adsorb sulfate in aqueous *** adsorbent was prepared by dropwise injection method where alginate droplets were crosslinked with barium to form hydrogel *** a typical scenario,the microcapsules had a diameter of 3 mm and contained 5 wt-%*** microcapsules could successfully reduce sulfate concentration in a solution from 100 to 16 mg·L^(−1),exceeding the removal ***,the microcapsules were mechanically unstable in the presence of an excess amount of ***,calcium ions were added as a secondary crosslinking agent to improve the integrity of the *** two-step Ca/Ba@alginate microcapsules showed an exceptional adsorption performance,reducing the sulfate concentration to as low as 0.02 mg·L^(−1).Since the sulfate selective microcapsules can be easily removed from the aqueous system and do not result in secondary barium contamination,these Ca/Ba@alginate adsorbents will find applications in ultra-refining of lithium in industry.

关键词： barium@alginate microcapsules dropwise injection sulfate removal lithium

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Erectile dysfunction and central obesity： an Italian perspective

引用

Asian Journal of Andrology 2014年第4期16卷 581-591页

作者： Giovanni Corona Giulia Rastrelli sandra Filippi Linda Vignozzi Edoardo Mannucci Mario Maggi Endocrinology Unit Maggiore-Bellaria Hospital Azienda-Usl Bologna Bologna Sexual Medicine and Andrology Unit Department of Experimental Clinical and Biomedical Sciences University of Florence Florence Diabetes Agency Careggi Hospital Florence Italy.

Erectile dysfunction （ED） is a frequent complication of obesity. The aim of this review is to critically analyze the framework of obesity and ED, dissecting the connections between the two pathological entities. Current clinical evidence shows that obesity, and in particular central obesity, is associated with both arteriogenic ED and reduced testosterone （T） levels. It is conceivable that obesity-associated hypogonadism and increased cardiovascular risk might partially justify the higher prevalence of ED in overweight and obese individuals. Conversely, the psychological disturbances related to obesity do not seem to play a major role in the pathogenesis of obesity-related ED. However, both clinical and preclinical data show that the association between ED and visceral fat accumulation is independent from known obesity-associated comorbidities. Therefore, how visceral fat could impair penile microcirculation still remains unknown. This point is particularly relevant since central obesity in ED subjects categorizes individuals at high cardiovascular risk, especially in the youngest ones. The presence of ED in obese subjects might help healthcare professionals in convincing them to initiate a virtuous cycle, where the correction of sexual dysfunction will be the reward for improved lifestyle behavior. Unsatisfying sexual activity represents a meaningful, straightforward motivation for consulting healthcare professionals, who, in turn, should take advantage of the opportunity to encourage obese patients to treat, besides ED, the underlying unfavorable conditions, thus not only restoring erectile function, but also overall health.

关键词： androgens erectile dysfunction impotence vasculogenic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diversity of dictyostelid social amoebae in high latitude habitats of Northern Sweden

引用

Fungal Diversity 2013年第1期58卷 185-198页

作者： Allison L.Perrigo sandra L.Baldauf Maria Romeralo Program in Systematic Biology Department of Organismal BiologyEvolutionary Biology CenterUppsala UniversityNorbyvägen 18D75236 UppsalaSweden

The dictyostelid social amoebae(Dictyostelia)occur in terrestrial habitats *** has been observed previously that their diversity decreases with increasing latitude and *** we look at dictyostelid diversity in the high latitude habitats of Northern *** were recovered from soil samples using traditional plating methods and then identified using morphological characters and molecular sequence(small subunit ribosomal RNA)*** total,nine species were recovered,including two new species,described herein as Dictyostelium barbibulus and Polysphondylium *** species diversity found here is discussed in relation to previous findings in the area as well as other high-latitude studies,and biogeographical patterns are *** total number of species found in Northern Sweden is lower than the numbers recorded for regions further south in Europe,a finding consistent with a latitudinal gradient of species *** findings highlight the benefit of using molecular data for accurate species identification in Dictyostelia and the need for a continued sampling effort to better understand their diversity and distribution,especially in high latitude habitats.

关键词： Cellular slime mold Dictyostelium Molecular identification New species Protist biogeography Sweden

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

UDP-glucuronosyltransferases mediate coffee-associated reduction of liver fibrosis in bile duct ligated humanized transgenic UGT1A mice

引用

Hepatobiliary Surgery and Nutrition 2021年第6期10卷 766-781页

作者： Steffen Landerer sandra Kalthoff Christian P.Strassburg Department of Internal Medicine I University Hospital BonnVenusberg-Campus 1BonnGermany

Background:Coffee consumption has been shown to reduce the risk of liver fibrosis and is capable of inducing human UDP-glucuronosyltransferase(UGT)1A ***1A enzymes act as indirect antioxidants catalyzing the elimination of reactive metabolites,which in turn are potent initiators of profibrotic *** aim of this study was to analyze the role of UGT1A genes as effectors of the protective properties of coffee in bile duct ligation(BDL)induced liver ***:Fourteen days BDL with and without coffee pre-and co-treatment was performed in htgUGT1A-WT and htgUGT1A-SNP *** UGT1A mRNA expression levels,serum bilirubin and aminotransferase activities were *** fibrosis was assessed by collagen deposition,computational analysis of Sirius red tissue staining and expression of profibrotic marker *** stress was measured by hepatic peroxidase concentrations and immunofluorescence ***:UGT1A transcription was differentially activated in the livers of htgUGT1A-WT mice after BDL,in contrast to a reduced or absent induction in the presence of ***-treated(coffee+BDL)htgUGT1A-WT-mice showed significantly increased UGT1A expression and protein levels and a considerably higher induction compared to water drinking WT mice(BDL),whereas in co-treated htgUGT1A-SNP mice absolute expression levels remained below those observed in htgUGT1A-WT *** deposition,oxidative stress and the expression of profibrotic markers inversely correlated with UGT1A expression levels in htgUGT1A-WT and SNP mice after BDL and coffee+BDL ***:Coffee exerts hepatoprotective and antioxidative effects via activation of UGT1A *** hepatic fibrosis as a result of coffee-mediated UGT1A induction during cholestasis was detected,while the protective action of coffee was lower in a common low-function UGT1A SNP haplotype present in 10%of the Caucasian *** study suggests that coffee consumption might

关键词： Glucuronidation cholestasis liver fibrosis coffee oxidative stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The effects of tannins-containing ground pine bark diet upon nutrient digestion,nitrogen balance,and mineral retention in meat goats

引用

Journal of Animal Science and Biotechnology 2015年第3期6卷 347-354页

作者： Byeng Ryel Min sandra Solaiman Thomas Terrill Aina Ramsay Irene Mueller-Harvey Department of Agricultural and Environmental Sciences Tuskegee University Agricultural Research Station Fort-Valley State University School of Agriculture Policy and DevelopmentUniversity of Reading

Background： Pine bark is a rich source of phytochemical compounds including tannins, phenolic acids, anthocyanins, and fatty acids. These phytochemicals have potential to significantly impact on animal health and animal production. The goal of this work is to measure the effects of tannins in ground pine bark as a partial feed replacement on feed intake, dietary apparent digestibility, nitrogen balance, and mineral retention in meat goats. Results： Eighteen Kiko cross goats （initial BW = 31.8 ± 1.49 kg） were randomly assigned to three treatment groups （n = 6）. Dietary treatments were tested： control （0 % pine bark powder （PB） and 30 % wheat straw （WS））; ] 5 % PB and 15 % WS, and 30 % PB and 0 % WS. Although dry matter （DM） intake and digestibility were not affected （P〉 0.10） by feeding PB, neutral detergent fiber （linear; P= 0.01）, acid detergent fiber （linear; P= 0.001） and lignin digestibility （linear; P= 0.01） decreased, and crude protein （CP） digestibility tended to decrease （P=0.09） as PB increased in the diet, apparent retention of Ca （P= 0.09）, P （P=0.03）, Mg （P= 0.01）, Mn （P= 0.01）, Zn （P= 0.01） and Fe （P= 0.09） also increased linearly. Nitrogen intake and fecal N excretion were not affected （P〉 0.05） by addition of PB in the diet, but N balance in the body was quadratically increased （P〈 0.01） in the 15 % PB diet compared to other diets. This may be due to more rumen escape protein and less excreted N in the urine with the 15 % PB diet. The study showed that a moderate level of tannin-containing pine bark supplementation could improve gastrointestinal nitrogen balance with the aim of improving animal performance. Conclusion： These results suggest that tannin-containing PB has negative impact on fiber, lignin, and protein digestibility, but positively impacted on N-balance.

关键词： Digestibility Goats Phytochemicals Tannins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Buckling analysis in stretchable electronics

引用

npj Flexible Electronics 2017年第1期1卷 30-38页

作者： Bo Wang Siyuan Bao sandra Vinnikova Pravarsha Ghanta Shuodao Wang School of Mechanical and Aerospace Engineering Oklahoma State UniversityStillwaterOK 74078USA School of Civil Engineering University of Science and Technology of SuzhouSuzhou 215011China

In the last decade,stretchable electronics evolved as a class of novel systems that have electronic performances equal to established semiconductor technologies,but can be stretched,compressed,and twisted like a rubber *** compliance and stretchability of these electronics allow them to conform and mount to soft,elastic biological organs and tissues,thereby providing attractive opportunities in health care and *** of stretchable electronic systems use an elastomeric substrate to carry an ultrathin circuit mesh that consists of sparsely distributed stiff,thin-film electronic components interconnected by various forms of stretchable metal strips or low-dimension *** the fabrication processes and application of stretchable electronics,the thin-film components or nanomaterials undergo different kinds of in-plane deformation that often leads to out-ofplane or lateral buckling,in-surface buckling,or a combination of all.A lot of creative concepts and ideas have been developed to control and harness buckling behaviors,commonly regarded as pervasive occurrences in structural designs,to facilitate fabrication of stretchable structures,or to enhance *** paper provides a brief review of recent progresses on buckling analysis in stretchable *** buckling mechanics reveals important correlations between the geometric/material properties and system performance(e.g.,mechanical robustness,deformability,structural architecture,and control).These mechanics models and analysis provide insights to design and optimize stretchable electronics for a wide range of important applications.

关键词： buckling rubber twisted

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：