Changes of the liver metabolome following an intravenous lipopolysaccharide injection in Holstein cows supplemented with dietary carnitine

作     者：Wei Xu Sandra Grindler Akos Kenez Sven Danicke Jana Frahm Korinna Huber Wei Xu;Sandra Grindler;Ákos Kenéz;Sven Dänicke;Jana Frahm;Korinna Huber

作者机构：Beijing Research Center of Intelligent Equipment for AgricultureBeijing 100097China. Department of BiosystemsBiosystems Technology ClusterCampus GeelKleinhoefstraat 42440 Geel LeuvenKUBelgium. Institute of Animal ScienceFaculty of Agricultural SciencesUniversity of Hohenheim70599 StuttgartGermany. Department of Infectious Diseases and Public HealthCity University of Hong KongBlock 14/FTo Yuen Building31 To Yuen StreetKowloonHong Kong SARChina. Institute of Animal NutritionFederal Research Institute for Animal Health(Friedrich-Loeffler-Institut)38116 BrunswickGermany. 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第1期

页      面：188-199页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：part of the MitoCow Consortium’s research,funded by the German Research Foundation(DFG Reference number:HU 838/12–2) 

主　　题：Acyl-carnitines Inflammatory response Lipid metabolism Liver metabolome Mitochondrial function 

摘      要：Background:Carnitine facilitates the flux of long-chain fatty acids for hepatic mitochondrial beta-oxidation,which acts to ameliorate the negative energy balance commonly affecting high-yielding dairy *** triggered by lipopolysaccharide(LPS)load can however pose a challenge to the metabolic integrity via the expression of pro-inflammatory mediators,leading to immune system activation and respective metabolic *** effect of enhanced carnitine availability on hepatic metabolome profiles during an inflammatory challenge has not yet been determined in dairy ***,Holstein cows were supplemented with 25 g/d rumen-protected carnitine from 42d prepartum until 126 d postpartum(n=16)or assigned to the control group with no supplementation during the same period(n=14).We biopsied the liver of the cows before(100 d postpartum)and after(112 d postpartum)an intravenous injection of 0.5μg/kg *** samples were subjected to a targeted metabolomics analysis using the AbsoluteIDQ p180 Kit(Biocrates Life Sciences AG,Innsbruck,Austria).Results:Multivariate statistical analyses revealed that hepatic metabolome profiles changed in relation to both the carnitine supplementation and the LPS *** the metabolite profiles on 100 d,carnitine increased the concentration of short-and long-chain acyl-carnitines,which may be explained by an enhanced mitochondrial fatty acid shuttle and hence greater energy *** LPS injection affected hepatic metabolite profiles only in the carnitine supplemented group,particularly altering the concentration of biogenic ***:Our results point to interactions between an acute hepatic inflammatory response and biogenic amine metabolism,depending on energy availability.