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Tea Polyphenols Reducing Lipopolysaccharide-induced Inflammatory Responses in RAW264.7 Macrophages via NF-κB Pathway

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Chemical Research in Chinese Universities 2019年第6期35卷 1105-1110页

作者： SU Siyi LI Xiaoyu GUO Xu ZHOU Ruiming LI Manman MING Pengfei HUANG Yingying sajid ur rahman DING Hongyan FENG Shibin LI Jinchun WANG Xichun LI Yu WU linjie College of Animal Science and Techmology Anhui Agricultural UniversityHefei 230036P.R.China

The anti-inflammatory activity of tea polyphenols(TPs)in RAW264.7 macrophages stimulated by lipopolysaccharide(LPS)was investigated in this ***264.7 macrophages were treated with different concentrations of TP(0,12.5,25,50,100,and 200μg/mL)and then stimulated by *** blank control group was set *** expression of pro-inflammatory cytokines associated with the nuclear factor-kappa B(NF-κB)signaling pathway was investigated before and after TP *** of RAW264.7 cells with TP decreased the expression of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin 1 beta(IL-1β)pro-inflammatory *** addition,TP inhibited the phosphorylation of p65 and IκB by blocking the phosphorylation and the degradation of NF-κB inhibitor *** conclusion,TP exerts anti-inflammatory effects by regulating the release of inflammatory mediators via the NF-κB signaling pathway.

关键词： Tea polyphenol Inflammatory response,RAW264.7 Nuclear factor-kappa B pathway

In-depth analysis of Bt cotton adoption:farmers'opinions,genetic landscape,and varied perspectives——a case study from Pakistan

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Journal of Cotton Research 2024年第3期7卷 277-288页

作者： SHAHZAD Rahil JAMIL Shakra CHAUDHRY urooj Fatima rahman sajid ur IQBAL Muhammad Zaffar Genetically Modified Organisms Development and Testing Laboratory Agricultural Biotechnology Research InstituteAyub Agricultural Research InstituteFaisalabadPunjab 38000Pakistan Centre of Excellence for Olive Research and Trainings(CEFORT) BaraniAgricultural Research InstituteChakwalPunjabPakistan

Background Bt technology has played significant role in controlling bollworms and increasing cotton yield in earlier days of its introduction,a subsequent decline in yield became apparent over *** decline may be attributed to various environmental factors,pest dynamics,or combination of ***,the present biophysical survey and questionnaire were designed to evaluate the impact of Bt cotton on bollworms management and its effect on reducing spray costs,targeting farmers with varied landholdings and educational ***,data on farmers'cultivated varieties and the prevalence of bollworms and sucking insects in their fields were ***,about eleven thousand cotton samples from farmer fields were tested for Cry1Ac,Cry2Ab and Vip3A genes by strip *** In this analysis,83% of the farmers planting approved varieties believe that Bt technology control bollworms,while 17% hold contradictory ***,among farmers cultivating unapproved varieties,77% agree on effectiveness of Bt technology against bollworms,while 23% *** the other hand,67% of farmers planting approved varieties believe that Bt technology does not reduce spray costs,while 33% agree with the ***,78% of farmers cultivating unapproved varieties express doubt regarding its role to reduce spray costs,while 22% are in favour of this *** in opinions on the effectiveness of Bt cotton in controlling bollworms and reducing spray cost between farmers planting unapproved and approved varieties may stem from several *** major cause is the heavy infestation of sucking insects,which is probably due to the narrow genetic variation of the cultivated ***,the widespread cultivation of unapproved varieties(21.67%)is also an important factor to cause different opinions on the effectiveness of Bt *** Based on our findings,we propose that the ineffective control of pests on cotton crop may

关键词： Bollworms Cry1Ac Cry2Ab Cotton Farmer's perception Purposive sampling Sucking insects Unapproved varieties Vip3A

Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

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Virologica Sinica 2024年第3期39卷 369-377页

作者： Shengyao Chen Muhammad Saqib Hafiz sajid Khan Yuan Bai Usman Ali Ashfaq Muhammad Khalid Mansoor Abulimti Moming Jing Liu Min Zhou Saifullah Khan Niazi Qiaoli Wu Awais-ur-rahman Sial Shuang Tang Muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat Mohsin Khurshid Iahtasham Khan Muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Faculty of Veterinary Science University of Agriculture FaisalabadFaisalabad 38040Pakistan The Children's Hospital Faisalabad 38000Pakistan Department of Bioinformatics and Biotechnology Government College University FaisalabadFaisalabad 38000Pakistan f Department of Microbiology Faculty of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Hubei Jiangxia Laboratory Wuhan 430200China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Armed Forces Institute of Pathology(AFIP) Rawalpindi 46000Pakistan Department of Clinical Studies Faculty of Veterinary&Animal SciencesPir Mehr Ali Shah Arid Agriculture UniversityRawalpindi 46300Pakistan Institute of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan l Atta-ur-rahman School of Applied Biosciences National University of Science and TechnologyIslamabad 44000Pakistan Department of Clinical Sciences University of Veterinary and Animal SciencesLahore Sub-campusJhang 35200Pakistan Department of Molecular Pathology National Medical Center DHA Phase 1Karachi 75500Pakistan

Infectious diseases caused by arboviruses are a public health concern in ***,studies on data prevalence and threats posed by arboviruses are *** study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive *** results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62%),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were ***,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing ***,individuals aged40 years,and outdoor workers had a high risk of exposure to *** these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple *** findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick-borne virus Mosquito-borne virus

A Fused Machine Learning Approach for Intrusion Detection System

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Computers, Materials & Continua 2023年第2期74卷 2607-2623页

作者： Muhammad sajid Farooq Sagheer Abbas Atta-ur-rahman Kiran Sultan Muhammad Adnan Khan Amir Mosavi School of Computer Science National College of Business Administration and EconomicsLahore54000Pakistan Department of Computer Science College of Computer Science and Information TechnologyImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam 31441Saudi Arabia Department of CIT The Applied CollegeKing Abdulaziz UniversityJeddah21589Saudi Arabia Department of Software Gachon UniversitySeongnam13120Korea John von Neumann Faculty of Informatics Obuda UniversityBudapest1034Hungary Institute of Information Engineering Automation and MathematicsSlovak University of Technology in BratislavaBratislava81107Slovakia Faculty of Civil Engineering TU-DresdenDresden01062Germany

The rapid growth in data generation and increased use of computer network devices has amplified the infrastructures of *** interconnectivity of networks has brought various complexities in maintaining network availability,consistency,and *** learning based intrusion detection systems have become essential to monitor network traffic for malicious and illicit *** intrusion detection system controls the flow of network traffic with the help of computer *** deep learning algorithms in intrusion detection systems have played a prominent role in identifying and analyzing intrusions in network *** this purpose,when the network traffic encounters known or unknown intrusions in the network,a machine-learning framework is needed to identify and/or verify network *** Intrusion detection scheme empowered with a fused machine learning technique(IDS-FMLT)is proposed to detect intrusion in a heterogeneous network that consists of different source networks and to protect the network from malicious *** proposed IDS-FMLT system model obtained 95.18%validation accuracy and a 4.82%miss rate in intrusion detection.

关键词： Fused machine learning heterogeneous network intrusion detection

A Novel Metadata Based Multi-Label Document Classification Technique

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Computer Systems Science & Engineering 2023年第8期46卷 2195-2214页

作者： Naseer Ahmed sajid Munir Ahmad Atta-ur rahman Gohar Zaman Mohammed Salih Ahmed Nehad Ibrahim Mohammed Imran BAhmed Gomathi Krishnasamy Reem Alzaher Mariam Alkharraa Dania AlKhulaifi Maryam AlQahtani Asiya A.Salam Linah Saraireh Mohammed Gollapalli Rashad Ahmed Barani Institute of Information Technology(BIIT) PMAS Arid Agriculture UniversityRawalpindi46000PunjabPakistan Department of Computer Science(CS) College of Computer Science and Information Technology(CCSIT)Imam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Faculty Computer Science and Information Technology Universiti Tun Hussein Onn MalaysiaBatu PahatMalaysia Department of Computer Engineering(CE) College of Computer Science and Information Technology(CCSIT)Imam Abdulrahman bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia College of Business Administration Imam Abdulrahman bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Department of Computer Information System(CIS) College of Computer Science and Information Technology(CCSIT)Imam Abdulrahman bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia ICS Department King Fahd University of Petroleum and MineralsDhahran31261Saudi Arabia

From the beginning,the process of research and its publication is an ever-growing phenomenon and with the emergence of web technologies,its growth rate is *** a rough estimate,more than thirty thousand research journals have been issuing around four million papers annually on *** engines,indexing services,and digital libraries have been searching for such publications over the ***,getting the most relevant articles against the user requests is yet a *** is mainly because the articles are not appropriately indexed based on the hierarchies of granular subject *** overcome this issue,researchers are striving to investigate new techniques for the classification of the research articles especially,when the complete article text is not available(a case of nonopen access articles).The proposed study aims to investigate the multilabel classification over the available metadata in the best possible way and to assess,“to what extent metadata-based features can perform in contrast to content-based approaches.”In this regard,novel techniques for investigating multilabel classification have been proposed,developed,and evaluated on metadata such as the Title and Keywords of the *** proposed technique has been assessed for two diverse datasets,namely,from the Journal of universal computer science(***)and the benchmark dataset comprises of the articles published by the Association for computing machinery(ACM).The proposed technique yields encouraging results in contrast to the state-ofthe-art techniques in the literature.

关键词： Multilabel classification indexing metadata content/data mining

QoS Parametric Inspection of Uniform and Assorted Trajectories for MANET Routing Protocols

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International Journal of Communications, Network and System Sciences 2017年第10期10卷 234-250页

作者： Nitesh Sehwani sajid rahman Anna Harris Departmentof Electronics and Telecommunications Engineering Sardar Patel Institute of Technology Mumbai India Department of Math and Computer Science University of Arkansas at Pine Bluff Pine Bluff AR USA

A Mobile Ad Hoc Network (MANET) is a self-governing network of mobile nodes without the inclusion of any wired links. Each node can move in an ad hoc manner and therefore, such a network should consist of routing protocols which can adapt to dynamically changing topologies. Numerous protocols have been proposed for the same. However, the trajectories followed by the individual nodes have not been distinctly dealt with. This paper presents a meticulous study on QoS parameters of proactive (OLSR) and reactive (DSR) protocols of MANETs for uniform as well as dissimilar trajectories of individual nodes in a small network of about 20 nodes. Also an examination of partial node failures for both the above mentioned protocols has been done. The performance metrics utilized in this study are average throughput and average delay. OPNET modeler has been utilized for this study. This assessment shows that for uniform trajectories, OLSR has almost same average delay but a higher average throughput as compared to DSR. Also it is seen that, as compared to uniform trajectories, non-uniform trajectories deliver a much higher average throughput. Node failures only reduce average throughputs whereas average delays remain unchanged.

关键词： MANET OLSR DSR Routing Throughput Delay

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沸石咪唑化合物ZIF-8的合成和吸附性能

沸石咪唑化合物ZIF-8的合成和吸附性能

作者： Arain sajid U rahman 北京化工大学

学位级别：硕士

水是一种必不可少的物质,是对地球生命的不可或缺条件。如今,全球正面临着严重的水质问题.各种工业活动的废水是造成环境污染的重要原因,因为工业废水中含有较高浓度的重金属离子。重金属由于其毒性,有很大的影响生物有机体.目前,用:于... 详细信息

水是一种必不可少的物质,是对地球生命的不可或缺条件。如今,全球正面临着严重的水质问题.各种工业活动的废水是造成环境污染的重要原因,因为工业废水中含有较高浓度的重金属离子。重金属由于其毒性,有很大的影响生物有机体.目前,用:于去除重金属离子的许多水处理技术是商业上应用的,例如化学沉淀,膜过滤,离子交换,电化学过程,和吸附过程,解决水问题。吸附是废水处理的主要方法之一。重金属去除有几个优点。多孔材料是经济的重要部分,通常考虑开发和设计多孔材料以具有通用性能。沸石咪唑酸酯骨架-8 ZIF-8材料由于其可行的结构和性能而获得了更多的兴趣,并且具有高的不同应用能力。在这项研究工作中,我们选用了 ZIF-8材料,它具有良好的晶体结构,如沸石材料,这是一种MOFs材料。我们专注于ZIF-8材料的合成,并赋予其去除重金属离子的吸附性能。最初,通过溶剂热法合成ZIF-8材料,其中含有锌作为金属离子和2-甲基咪唑作为配体,二甲基甲酰胺用于修饰反应的演化。使用了各种表征技术,如XRD,SEM和FT-IR,探讨合成ZIF-8样品的特性.在吸附过程中研究去除离子废水中重金属离子来探究合成ZIF-8的吸附性能。结果表明ZIF-8被提出作为去除重金属离子(HMI)的有效吸附材料,包含Zn,Cu,Pb,Cd和Ni.此外我们也探究了一些重要的操作参数的影响(溶液的pH值,浓度HMIs,吸附时间,和吸附剂用量)吸附性能研究(吸附容量和去除率)也被调查。所以,它解释了当溶液的pH值很高时,功能组的质子化程度很强,竞争离子H降低,化学复合物相互作用增加。因此,ZIF-8具有很高的吸附量大的重金属离子HMIs的能力。平衡吸附时间Cu,Zn,Pb,Cd和Ni分别为 25,5,15,5 45 和 10 h,HMI 的饱和吸附容量分别为 481.9,249.6,1184.8,812.8 和 247.2 mg·g,分别.我们将这一结果与文献比较,发现该结果优于天然和人造吸附剂,如活性炭,沸石,稻壳,泥炭等。ZIF-8和ZIF-8与离子粉末的XRD和FT-IR表征,揭示吸附过程前后晶体结构发生变化,并表明吸附是离子交换层析,也可能发生化学复合物相互作用。因此,发现物理吸附除外还有化学吸附。在这部分研究中,初步吸附机理证明,将实验数据与吸附等温线和动力学方程相关联,并检查吸附过程中的离子交换。通过这项研究,我们知道吸附过程与朗缪尔模型有很好的相关性。五个金属离子的K顺序是,Pb>Zn>Cu>Cd>Ni,饱和吸附容量接近于相关结果,所以,这意味着实验结果与模型一致。在这部分中,通过测量吸附溶液中Zn离子的浓度来研究吸附过程中的离子交换强度,然后检查离子交换强度的增加,在吸附过程中随着重金属离子浓度的增大而变光滑。当重金属离子HMI的初始浓度固定值为300 mg·L时,然后,离子交换强度的顺序是 Pb>Cu>Ni>Cd.

关键词：合成 ZIF-8 去离子水重金属离子吸附过程

Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

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Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-rahman Sial Saeed Khan Zeeshan Taj Halima Sadia Usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan

Analysis the Influenced of Plain and Twill Structures on Woven Fabric Properties

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Journal of Textile Science and Technology 2020年第3期6卷 107-113页

作者： Monour Khan Hasan Mahmud Tirtha Sarathi Das Muhammad Abdullah Tushar rahman Khan Rokibul Hasan Shohan sajid Al Rafi Md Solaiman Department of Fabric Manufacturing Engineering Textile Engineering College Noakhali Affiliated by Bangladesh University of Textiles Dhaka Bangladesh Department of Wet Processing Engineering Textile Engineering College Noakhali Affiliated by Bangladesh University of Textiles Dhaka Bangladesh

Fabric outer appearance is influenced by various fabric structures. In this research work, the physical properties of plain and twill were analyzed. Physical, mechanical and other aesthetic properties are also tested and the finding results are also compared between two samples. After testing the samples it is found that rubbing fastness remains unchanged for plain and twill fabric. Abrasion resistance, pilling, crease recovery and cover factor were evaluated for twill and plain structures using ISO 12945-2, ISO 12945-1 and ISO 2313 methods respectively. Abrasion resistance and pills property are not good for twill fabric in comparison with plain fabric but crease recovery is better of twill fabric.

关键词： Abrasion Pilling Crease Recovery Color Fastness Cover Factor EPI & PPI