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Antifungal activity of four honeys of different types from Algeria against pathogenic yeast:Candida albicans and Rhodotorula sp.

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Asian Pacific Journal of Tropical Biomedicine 2012年第7期2卷 554-557页

作者： Ahmed Moussa Djebli Noureddine aissat saad Meslem Abdelmelek Benhalima Abdelkader Institute of Veterinary Sciences University Department of Biology Faculty of SciencesWostaganem University Laboratory Science and Technology Environment and Development Mostaganem University

Objective:To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast *** albicans(***) and Rhoddtorula ***:Four Algeria honeys of different botanical origin were analvzed to test anlilungal effect against ***,and Rhodotorula *** concentrations(undiluted,10%,30%,50%and 70%w/v) of honey were studied in vitro for their antifugal aclivity using *** and Rhodotorula *** fungal ***:The range of the c liameter of zone of inhibition of various concentrations ol tested honeys was(7-23 mm) for Rhodotorula sp.,while *** showed clearly resistance towards all concentrations *** MICs of tested honey concentrations against *** and Rhodotorula ***(70.09-93.48)%and(4.90-99.70)%v/v,***:This study demonstrales that,in vitro,these natural products have clearly an antifungal activity against Rhodotorula *** ***.

关键词： Honey Antifungal activity Candida albicans Rhodotorula sp.

In vitro activity of natural honey alone and in combination with curcuma starch against Rhodotorula mucilaginosa in correlation with bioactive compounds and diastase activity

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Asian Pacific Journal of Tropical Biomedicine 2013年第10期3卷 816-821页

作者： Moussa Ahmed Noureddine Djebli saad aissat Baghdad Khiati Abdelmalek Meslem Salima Bacha Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem.UniversityAlgeria

Objective:To evaluate the in vitro activity and synergism of the combinations of natural honey and curcuma starch against Rhodotorula mucilaginosa in correlation with total phenolic,flavonoid contents,and diastase ***:The Folin-Ciocalteu test was used to determine the total polyphenols content and the flavonoid content was analyzed using by the aluminum chloride *** antifungal activity of the natural honey,determined by an agar well diffusion assay and agar incorporation ***:Total phenolic content varied from(63.93±0.11)to(95.36±6.08)mg GAE/100 g honey as gallic acid *** flavonoids content varied from(5.41±0.04)to(9.94±0.54)mg CE/*** activity values were between(7.3±2.8)and(26±2.8).The zone inhibition diameter for the six honey samples without starch ranged between 6 and 20 *** starch was mixed with honey and then added to well,a zone inhibition increase diameter 7 and 21 *** percentage increase was noticed with each variety and it ranged between 5%and 62.5%.The minimal inhibitory concentrations for the six varieties of honey without starch against Rhodotorula mucilaginosa ranged between 28%and 36%(v/v).When starch was incubated with honey and then added to media,a minimal inhibitory concentration drop has been noticed with each *** ranged between 6.66%and 20%(w/v).No significant correlation was established between diastase activity and bioactive ***:The mixture of curcuma starch and honey could lead to the development of new combination antibiotics against Rhodotorula

关键词： Bioactive Compounds Diastase number Rhodotorula mucilaginosa Honey Curcuma starch

Additive potential of ginger starch on antifungal potency of honey against Candida albicans

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Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 253-255页

作者： Ahmed Moussa Djebli Noureddine Hammoudi SM aissat saad Akila Bourabeh Hemida Houari Laboratory of Hygiene and Animal Pathology Institute of Veterinary Sciences University Ibn-KhadounTiaret Departments of Biology Faculty of SciencesMostaganem University Institute of Veterinary Sciences University Ibn-Khadoun Tiaret

Objective:To evaluate the additive action of ginger starch on the antifungal activity of honey against Candida albicans(***).Methods:*** was used to determine the minimum inhibitory concentration(MIC)of four varieties of Algerian *** concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to detennine the minimum additive inhibitory concentration(MAIC).Results:The MIC for the four varieties of honey without starch against *** ranged between 38%and 42%(v/v).When starch was incubated with honey and then added to media,a MIC drop was noticed with each *** of the four varieties ranged between 32%honey(v/v)with 4%starch and 36%honey(v/v) with 2%***:The use of ginger starch allows honey benefit and will constitute an alternative way against the resistance to antifungal agents.

关键词： Honey Ginger starch Antifungal potency Candida albicans

Effect of heat treatment on antimycotic activity of Sahara honey

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Journal of Coastal Life Medicine 2014年第11期 876-881页

作者： Moussa Ahmed saad aissat Noureddine Djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To evaluate the influence of the temperature on honey colour,polyphenol contents and antimycotic capacity and to evaluate the correlation between these ***:Sahara honey were heated up to 25,50,75 and 100℃for 15,30 and 60 min,and their colour intensity,polyphenol contents and antimycotic *** Folin-Ciocalteu test was used to determine the total polyphenol contents(TPC).The antimycotic activity was evaluated both by agar diffusion method and micro wells dilution method against the Candida albicans(***)and Candida glabrata(***).Results:Initial values for TPC in Sahara honey ranged from 0.55 to 1.14 mg of gallic acid per kg of honey,with the average value of 0.78 mg of gallic acid per kg of *** TPC values after heat-treatment were 0.54 to 1.54 with the average value of 1.49 *** minimal inhibitory concentrations before heat-treatment of Sahara honey against *** and *** ranged from 3.06%-12.5%and 50%*** heat-treatment the minimal inhibitory concentrations between 12.5%and 50%for *** and ***,*** diameters of inhibition zones of Sahara honey with 50%concentration varied from(12.67-15.00)mm by *** to(14.33-15.67)mm by *** diameters of inhibition zones after heat-treatment at 25 and 50°C for 15.30 and 60 min ranged from(2.00-18.67)mm by *** to(8.00-16.67)mm by *** significant relations between the TPC and the colour intensity of Sahara honey(r=0.99,P<0.05).Furthermore,the results showed that the TPC and colour is not correlated with the antimycotic ***:To our knowledge this is the first report on the antimycotic capacity of Sahara honey.

关键词： Sahara honey Antimycotic capacity Heat treatment

Preliminary comparative study of anti-inlfammatory effect of unheated and heat-treated Sahara honey:In vivo approach

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Journal of Coastal Life Medicine 2015年第11期 875-878页

作者： Moussa Ahmed saad aissat Noureddine Djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To investigate the effect of unheated and heat-treated of Sahara ***:A total of 24 Swiss albino mice weighing 25-35 g were divided into four groups(n=6).Anti-inflammatory effect was assessed at 1,2,3,4,5 and 6 h after subplantar injection of carrageenan(0.5 mL of a 1%solution in normal saline).In addition,total phenolic content was determined by modified Folin-Ciocalteu ***:The total phenolic content capacity of the Sahara honey before and after heat treatment was between 72 and 97.9 mg of gallic acid equivalents/100 g of honey *** of unheated honey(oral administration)reduced significantly(P<0.05).The carrageenan induced mice paw edema model at 1,3 and 6 h for 21.85%,5.43%and 80.43%,*** of heat-treated honey showed insignificant inhibition of carrageenan and induced paw edema at 1 h(31.16%),3 h(0.25%)and 6 h(34.19%).The 50 mg/kg diclofenac exhibited percent reduction in paw volume 16.12%,8.90%and 15.32%after 1 h,3 h and 6 h,respectively,when compared with control *** toxicity was ***:Our results suggest that unheated Sahara honey has anti-inflammatory effects by reducing the mice paw edema size while heat-treated Sahara honey decreases the antiinflammatory activity.

关键词： Sahara honey Heating Anti-inflammatory Total phenolic content

Preliminary study on synergistic combinations of raw honey with gentamicin against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa of veterinary origin

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Journal of Coastal Life Medicine 2015年第5期 366-369页

作者： Moussa Ahmed Baghdad Khiati saad aissat Noureddine Djebli Pharmacognosy Api-Phytotherapy Laboratory Mostaganem UniversityMostaganemAlgeria Institute of Veterinary Sciences University Ibn-Khaldoun TiaretAlgeria

Objective:To search for further synergistic combinations of gentamicin and raw honey that might have potential in treating ***:The antibacterial activity and synergistic interaction of raw honey and gentamicin was assessed by using agar well diffusion *** Gram-negative(Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 2154)bacteria were selected for antibacterial activity *** cultures of bacteria were maintained in their appropriate agar slants at 4°C throughout the study and used as stock ***:Raw honey and gentamicin interacted synergistically to inhibit Escherichia coli and Pseudomonas ***:These results suggest that combinations of raw honey and gentamicin have therapeutic benefits in prophylaxis of infections caused by multidrug-resistant Gram-negative bacilli.

关键词： Honey Gentamicin Pseudomonas aeruginosa Escherichia coli

Effect of honey on oxidation, chlorination and nitration by purified equine myeloperoxidase

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Journal of Coastal Life Medicine 2017年第9期5卷 398-402页

作者： saad aissat Hama Benbarek Thierry Franck Stephan Kohnen Didier Serteyn Moussa Ahmed Ange Mouithys-Mickalad Institute of Veterinary Sciences Ibn Khaldoun UniversityTiaret14000Algeria Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria Faculty of Sciences of Nature and Life Mascara UniversityMascara29000Algeria Centre for Oxygen Research and Development(CORD)Institute of Chemistry B6aUniversity of LiègeSart Tilman4000Liège1Belgium Department of Clinical Sciences Large Animal SurgeryFaculty of Veterinary MedicineB41University of LiègeSart Tilman4000Liège1Belgium Celabor Centre de Services Scientifique et Technique aux EntreprisesZoning Industriel de Petit-RechainAvenue du Parc384650HerveBelgium

Objective: To evaluate the antioxidant effect of honey using two classical methods generally used, and for the first time to test the effect of honey on the oxidation, chlorination and nitration by purified equine myeloperoxidase (MPO). Methods: The antioxidant activity of three Algerian honey samples (nectar honey, mixed honey and honeydew honey) was evaluated by two classical methods, the ferric- reducing/antioxidant power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity. Results: Honeydew honey had the highest reducing power and DPPH radical-scavenging activity, whereas nectar honey showed the lowest reducing power and DPPH radical-scavenging activity. All honey samples showed a significant inhibitory effect on the chlorination activity of equine MPO, but honeydew honey was the weakest inhibitor. The three samples were poorly inhibitor on the MPO oxidation and nitration activities, except for nectar honey that exerted an inhibitory effect at the highest tested concentration of 10%. These later results seem to contradict those obtained with DPPH and FRAP. Conclusions: The antioxidant capacity of honey is mainly due to the phenolic compounds and flavonoids it contained. It has been suggested that MPO might be involved in the antioxidant, not pro-oxidant, activity of phenolic compounds.

关键词： Honey Myeloperoxidase Chlorination Oxidation Nitration

An Enhanced Lung Cancer Detection Approach Using Dual-Model Deep Learning Technique

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 835-867页

作者： Sumaia Mohamed Elhassan saad Mohamed Darwish Saleh Mesbah Elkaffas Department of Information Technology Institute of Graduate Studies and ResearchAlexandria UniversityAlexandria21526Egypt College of Computing and Information Technology Arab Academy for ScienceTechnology and Maritime TransportAlexandriaP.O.Box 1029Egypt

Lung cancer continues to be a leading cause of cancer-related deaths worldwide,emphasizing the critical need for improved diagnostic *** detection of lung tumors significantly increases the chances of successful treatment and ***,current diagnostic methods often fail to detect tumors at an early stage or to accurately pinpoint their location within the lung ***-model deep learning technologies for lung cancer detection,while beneficial,cannot capture the full range of features present in medical imaging data,leading to incomplete or inaccurate ***,it may not be robust enough to handle the wide variability in medical images due to different imaging conditions,patient anatomy,and tumor *** overcome these disadvantages,dual-model or multi-model approaches can be *** research focuses on enhancing the detection of lung cancer by utilizing a combination of two learning models:a Convolutional Neural Network(CNN)for categorization and the You Only Look Once(YOLOv8)architecture for real-time identification and pinpointing of *** automatically learn to extract hierarchical features from raw image data,capturing patterns such as edges,textures,and complex structures that are crucial for identifying lung ***8 incorporates multiscale feature extraction,enabling the detection of tumors of varying sizes and scales within a single *** is particularly beneficial for identifying small or irregularly shaped tumors that may be challenging to ***,through the utilization of cutting-edge data augmentation methods,such as Deep Convolutional Generative Adversarial Networks(DCGAN),the suggested approach can handle the issue of limited data and boost the models’ability to learn from diverse and comprehensive *** combined method not only improved accuracy and localization but also ensured efficient real-time processing,which is crucial for practical clinical applicati

关键词： Lung cancer detection dual-model deep learning technique data augmentation CNN YOLOv8

Global Piecewise Analysis of HIV Model with Bi-Infectious Categories under Ordinary Derivative and Non-Singular Operator with Neural Network Approach

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 609-633页

作者： Ghaliah Alhamzi Badr saad TAlkahtani Ravi Shanker Dubey Mati ur Rahman Department of Mathematics and Statistics College of ScienceImam Mohammad Ibn Saud Islamic University(IMSIU)Riyadh13318Saudi Arabia Department of Mathematics College of ScienceKing Saud UniversityRiyadh11989Saudi Arabia Department of Mathematics Amity School of Applied SciencesAmity University RajasthanJaipur302002India School of Mathematical Sciences Jiangsu UniversityZhenjiang212013China Department of Computer Science and Mathematics Lebanese American UniversityBeirut13-5053Lebanon

This study directs the discussion of HIV disease with a novel kind of complex dynamical generalized and piecewise operator in the sense of classical and Atangana Baleanu(AB)derivatives having arbitrary *** HIV infection model has a susceptible class,a recovered class,along with a case of infection divided into three sub-different levels or categories and the recovered *** total time interval is converted into two,which are further investigated for ordinary and fractional order operators of the AB derivative,*** proposed model is tested separately for unique solutions and existence on bi *** numerical solution of the proposed model is treated by the piece-wise numerical iterative scheme of Newtons *** proposed method is established for piece-wise derivatives under natural order and non-singular Mittag-Leffler *** cross-over or bending characteristics in the dynamical system of HIV are easily examined by the aspect of this research having a memory effect for controlling the said *** study uses the neural network(NN)technique to obtain a better set of weights with low residual errors,and the epochs number is considered *** obtained figures represent the approximate solution and absolute error which are tested with NN to train the data accurately.

关键词： HIV infection model qualitative scheme approximate solution piecewise global operator neural network