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Enzymatic and Ultrastructural Studies in a Freshwater Catfish: Impact of Methyl parathion

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Biomedical and Environmental Sciences 1990年第2期3卷 166-182页

作者： G.TRIpATHI S.p.shukla Department of Zoology Banaras Hindu University Varanasi 221005 India

Exposure to a sublethal concentration of methyl parathion (MEp) reduced the activity of cytoplasmic malate dehydrogenase. mitochondrial malate dehydrogenase, and lactate dehydrogenase by 30 to 49% in the liver and the skeletal muscle of the freshwater catfish, Clarias batraclms, after 7 days. The activities then began to recover and reached the control levels on the 28th day of MEp exposure. A complete recovery occurred on the 7th day when MEp was withdrawn from the medium after an exposure for 1 week. The withdrawal-dependent recovery in the activities was inhibited partially or completely by actinomycin Dandcycloheximide, suggesting de nova synthesis of the enzymes during the recovery period. A conjoint treatment of MEp and triiodolhyronine (T_3) restored the activities to control levels, indicating T_3 protection against the pesticide toxicity. SDS-pAGE of the cytoplasmic fraction of the liver showed some noticeable changes in the protein pattern after an exposure to MEp. Ultrastructural studies on MEp-treated liver cells showed disappearance of the glycogen granules and appearance of numerous smooth endoplasmic reticulum, lysosomal dense bodies, and swollen mitochondria. These changes in the liver are an indication of hepatic toxicity leading toward necrosis.(c) 1990 Academic ***.

关键词： Enzymatic and Ultrastructural Studies in a Freshwater Catfish MEp

Malate and Lactate Dehydrogenases of a Freshwater Catfish: Impact of Endosulfan

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Biomedical and Environmental Sciences 1990年第1期3卷 52-64页

作者： G. TRIpATHI S. p. shukla Department of Zoology Banaras Hindu University Varanasi 221005 India

A sublethal concentration of technical grade endosulfan (END) inhibited 35 to 55% of the activities of cytoplasmic malate dehydrogenase (cMDH), mitochondrial malate dehydrogenase (mMDH), and lactate dehydrogenase (LDH) in the liver and the skeletal muscle of a freshwater catfish, Clarias halrachus, after 7 days of exposure. The activity remained in the inhibited state up to 28 days. The withdrawal of END from the medium after 1 week of exposure gradually restored the activities to control levels within 21 days in the skeletal muscle and 28 days in the liver. The administration of actinomycin D or cycloheximide between the 14th and the 21st day of the withdrawal of END almost completely inhibited the withdrawal-dependent recovery in the activities of all the three enzymes. This indicates de novo synthesis of the enzymes during the recovery period. A conjoint treatment of END and triiodothyronine (T_3) raised the activities of cMDH, mMDH, and LDH in the liver and the skeletal muscle up to the control levels. This shows that the inhibitory effect of END may be relieved in presence of T_3. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed few changes in the pattern of cytoplasmic proteins of the liver and the skeletal muscle in response to exposure to END. 1990 Academic press. Inc.

关键词： ND Impact of Endosulfan Malate and Lactate Dehydrogenases of a Freshwater Catfish

phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean(Indian sector)water

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Acta Oceanologica Sinica 2015年第8期34卷 73-82页

作者： GUpTA pratibha BALAJI Raju pARANI M CHANDRA T S shukla p KUMAR Anil BANDOpADHYAY Rajib Department of Bio-Engineering Birla Institute of Technology Department of Genetic Engineering Kattankulathur Campus SRM University Microbiology and Genetics Laboratory Department of Biotechnology Indian Institute of Technology Madras Department of Microbiology Maharshi Dayanand University OSSG Department National Centre for Antarctic and Ocean Research Ministry of Earth Sciences Government of India UGC Centre of Advanced Study Department of Botany the University of Burdwan

Fifty-seven bacteria were isolated from Southern Ocean （Indian sector） water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4℃, 20℃, 37℃ and tolerable NaCI concentration up to 13.5% （w/v）. 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information （NCBI）. All the isolates were classified by using Ribosomal Database project （RDp） and found that isolates belongs to proteobacteria and Bacteriodes. The average G＋C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium （NCIM 2501）, Staphylococcus aureus （NCIM 2122）, Bacillus subtilis （NCIM 2193）, and pseudomonas aeruginosa （NCIM 2036）. Nine out of 29 were found to be antibiotic producer.

关键词： Southern Ocean marine bacteria 16S rDNA phylogenetic tree antibacterial

predicting suitability of different scale-dependent dispersivities for reactive solute transport through stratified porous media

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Journal of Rock Mechanics and Geotechnical Engineering 2016年第6期8卷 921-927页

作者： Deepak Swami Abhimanyu Sharma pramod Kumar Sharma Dericks p.shukla School of Engineering Indian Institute of Technology Mandi School of Engineering Indian Institute of Technology Roorkee

In this paper,the behavior of breakthrough curves(BTCs) for reactive solute transport through stratified porous media is investigated.A physical laboratory model for layered porous media was constructed,in which thin layer of gravel was sandwiched in between two thick layers of natural *** layer and natural soil layers were hydraulically connected as single porous continuum.A constant source of tracer was connected through gravel layer and elucidated at different sampling points in the direction of *** multiprocess non-equilibrium(MpNE) transport equation with scale-dependent dispersivity function was used to simulate experimental BTCs of reactive solute transport through layered porous *** values of equilibrium sorption coefficient and other input parameters were obtained *** simulation of BTC was performed using MpNE model with scale-dependent *** simulation of different scale-dependent dispersivities was then compared and it was found that for field scale of estimation of dispersivity,asymptotic and exponential dispersivity functions performed *** continuation to the comparison of simulated BTCs obtained using different models,spatial moment analysis of each aforesaid scale-dependent dispersivity model was also *** moment analysis provides the information related to mean solute mass,rate of mass travel,and mean plume *** and constant dispersivities showed higher variance as compared to asymptotic and exponential dispersion *** supports the field applicability of asymptotic and exponential dispersivity *** BTCs were also found to elucidate a nonzero concentration with time,which was clearly affected by physical *** natural condition,such information is required in effective aquifer remediation process.

关键词： Multiprocess non-equilibrium(MpNE) Scale-dependent dispersivity Stratified porous media Spatial moment

A Clean Technology for Future prospective: Emission Modeling of Gas Based power plant

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Open Journal of Air pollution 2016年第4期5卷 144-159页

作者： Markandeya S.p.shukla G.C.Kisku Department of Civil Engineering Institute of Engineering and Technology Lucknow India Environmental Monitoring Laboratory Environmental Toxicology Group CSIR-Indian Institute of Toxicology Research (CSIR-IITR) Lucknow India

The aim of present research is to study the dispersion of air pollutants using the air quality model, AERMOD and to predict the impact of pollutants (pM10, NO2 and CO) at the receptor level released from Gas Based power plant (GBpp). The net-concentrations including monitored data plus predicted values of pM10, NO2 and CO would be increased from base value 75 to 77.61 μg/mp>3p> with an increase of 3.48%, 22 to 26.66 μg/mp>3p> with an increase of 21.18% and 428 to 538.37 μg/mp>3p> with an increase of 25.79% respectively. The study of hill effect showed that it had profound impact upon the dispersion of pollutants and the ratio (with hill and without hill) of each pollutant was 3.89 for pM10 (24 hr), 2.40 for NO2 (24 hr) and 13.98 for CO (1 hr). The natural gas based plant not only decreases the pollution level but also reduces the hospital treatment cost and protects the public health. The modeling results suggest that the GBpp could be a clean technology as replacement of coal power plants located in the city which pollute the environment considerably in spite of control measures installed.

关键词： Gas Based power plant AERMOD Fine particulates and Gaseous pollutants Health Effects

Southern and Tropical Indian Ocean SST: A possible predictor of Winter Monsoon Rainfall over South India

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Atmospheric and Climate Sciences 2013年第4期3卷 440-449页

作者： Ravi p. shukla Shailendra Rai Avinash C. pandey Centre for Ocean-Land-Atmosphere Studies Calverton USA K. Banerjee Center of Atmospheric & Ocean Studies Institute of Interdisciplinary Studies

The complexities in the relationship between winter monsoon rainfall (WMR) over South India and Sea Surface temperature (SST) variability in the southern and tropical Indian Ocean (STIO) are evaluated statistically. The data of the time period of our study (1950-2003) have been divided exactly in two halves to identify predictors. Correlation analysis is done to see the effect of STIO SST variability on winter monsoon rainfall index (WMRI) for South India with a lead-lag of 8 seasons (two years). The significant positive correlation is found between Southern Indian Ocean (SIO) SST and WMRI in July-August-September season having a lag of one season. The SST of the SIO, Bay of Bengal and North Equatorial Indian Ocean are negatively correlated with WMRI at five, six and seven seasons before the onset of winter monsoon. The maximum positive correlation of 0.61 is found from the region south of 500 S having a lag of one season and the negative correlations of 0.60, 0.53 and 0.57 are found with the SST of the regions SIO, Bay of Bengal and North Equatorial Ocean having lags of five, six and seven seasons respectively and these correlation coefficients have confidence level of 99%. Based on the correlation analysis, we defined Antarctic Circumpolar Current Index A and B (ACCIA (A) & ACCIB (B)), Bay of Bengal index (BOBI (C)) and North Equatorial Index (NEI (D)) by averageing SST for the regions having maximum correlation (positive or negative) with WMRI index. These SST indices are used to predict the WMRI using linear and multivariate linear regression models. In addition, we also attempted to detect a dynamic link for the predictability of WMRI using Nino 3.4 index. The predictive skill of these indices is tested by error analysis and Willmott’s index.

关键词： Winter Monsoon Rainfall over South India Southern/Tropical Indian Ocean Multivariate/Linear Regression Models

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最大量生产液体三氧化硫和发烟硫酸

硫磷设计与粉体工程 1995年第2X期 38-43页

作者： Goel A p shukla S p 孙正东印度孟买的poseidon私营设计咨询有限公司

硫酸厂生产液体三氯化硫、发烟硫酸等高附加值产品是硫酸工业的一种新趋势。结合Transpek工程介绍印度poseidon公司生产液体三氧化硫和65％发烟硫酸的工艺技术、设计概念和Transpek工程运行状况。工艺技术特点为:工艺空气经处理温度降... 详细信息

硫酸厂生产液体三氯化硫、发烟硫酸等高附加值产品是硫酸工业的一种新趋势。结合Transpek工程介绍印度poseidon公司生产液体三氧化硫和65％发烟硫酸的工艺技术、设计概念和Transpek工程运行状况。工艺技术特点为:工艺空气经处理温度降到20℃;(4+1)两转两吸工艺,转化器四段出口含SO_3气体进发烟硫酸系统;优化装置能量利用;可按要求的比率最大量地生产液体三氯化硫和65％发烟硫酸。

关键词：发烟硫酸液体三氧化硫蒸发器进塔温度吸收塔最大量产品方案工艺流程硫酸工业两转两吸工艺

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对防治芒果叶蝉的杀虫剂的评价

热带作物译丛 1986年第3期 35-37页

作者： R.p.shukla 杨光融

在印度及许多地区,芒果叶蝉(Tdioscopus clypealis)是芒果的毁灭性害虫。在印度卡纳塔克进行的调查表明,在芒果开花季节,这种害虫非常严重,损失达20—100％。久效磷、西维因和磷胺防治这种叶蝉效果良好,但尚未报道过合成除虫菊酯类的防... 详细信息

在印度及许多地区,芒果叶蝉(Tdioscopus clypealis)是芒果的毁灭性害虫。在印度卡纳塔克进行的调查表明,在芒果开花季节,这种害虫非常严重,损失达20—100％。久效磷、西维因和磷胺防治这种叶蝉效果良好,但尚未报道过合成除虫菊酯类的防效。本试验比较4种除虫菊酯以及触杀剂,内吸剂防治此蝉的经济效益和药效。

关键词：西维因 0-(1-萘基)-N-甲基氨基甲酸酯有机氮杀虫剂叶蝉久效磷磷胺芒果

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芒果声蚜的化学防治

世界热带农业信息 1985年第6期 40-40页

作者： R.p.shukla 陈俊学

据Butani(1979)报道,芒果声蚜(Toxoptera odinae Vander Goot)曾是芒果的一种次要害虫,但目前,它已成为印度卡纳塔克邦芒果开花季节的一种固定害虫。声蚜的成虫与若虫吸食嫩枝叶的汁液,

关键词：磷胺乐果乐戈久效磷马拉硫磷卢比