Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean(Indian sector)water

作     者：GUPTA Pratibha BALAJI Raju PARANI M CHANDRA T S SHUKLA P KUMAR Anil BANDOPADHYAY Rajib 

作者机构：Department of Bio-Engineering Birla Institute of Technology Department of Genetic Engineering Kattankulathur Campus SRM University Microbiology and Genetics Laboratory Department of Biotechnology Indian Institute of Technology Madras Department of Microbiology Maharshi Dayanand University OSSG Department National Centre for Antarctic and Ocean Research Ministry of Earth Sciences Government of India UGC Centre of Advanced Study Department of Botany the University of Burdwan 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2015年第34卷第8期

页      面：73-82页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：the Expedition support to MoES, New Delhi and NCAOR, Goa (No. Mo ES/NCAOR/SOS/1/2007-PC-I dated January 4, 2011) the Cumulative Professional Development Grant (CPDG Ref No. GO/PD/2011-12/269/3523 dated, August 04, 2011) from BIT, Mesra BTISNet Sub DIC (BT/BI/065/2004) for providing internet facilities and the Government of Jharkhand Department of Agriculture for providing infrastructure development fund (5/B.K.V/Misc/12/2001) the financial support as research fellowship to Centre of Excellence (COE) (Ref No. NPIU/TEQIP II/FIN/31/158, dated April 16, 2013) at the Department of BioEngineering 

主　　题：Southern Ocean marine bacteria 16S rDNA phylogenetic tree antibacterial 

摘      要：Fifty-seven bacteria were isolated from Southern Ocean （Indian sector） water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4℃, 20℃, 37℃ and tolerable NaCI concentration up to 13.5% （w/v）. 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information （NCBI）. All the isolates were classified by using Ribosomal Database Project （RDP） and found that isolates belongs to Proteobacteria and Bacteriodes. The average G＋C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium （NCIM 2501）, Staphylococcus aureus （NCIM 2122）, Bacillus subtilis （NCIM 2193）, and Pseudomonas aeruginosa （NCIM 2036）. Nine out of 29 were found to be antibiotic producer.