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Gapless Genome Assembly of ZH8015 and preliminary Multi-Omics Analysis to Investigate ZH8015's Responses Against Brown planthopper Infestation

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Rice science 2024年第3期31卷 317-327,I0042-I0045页

作者： LI Dian DUAN Wenjing LIU Qun’en WU Weixun ZHAN Xiaodeng sUN Lianping ZHANG Yingxin cHenG shihua state Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China

Accurate genomic information is essential for advancing genetic breeding research in specific rice *** study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using pac Bio HiFi,Hi-c,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene ***,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015's response to brown planthopper(BpH)*** transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BpH *** analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall *** results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid *** association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015's response to BpH *** summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.

关键词： brown planthopper gapless genome genome assembly multi-omics Nilaparvata lugens rice

Radiative decays D*(s)→ D(s)γ in covariant confined quark model

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chinese physics c 2024年第2期48卷 45-56页

作者： c.T.Tran M.A.Ivanov p.santorelli Q.c.Vo Department of physics HCMC University of Technology and EducationVo Van Ngan 1700000 Ho Chi Minh CityVietnam Bogoliubov Laboratory of Theoretical physics Joint Institute for Nuclear Research141980 DubnaRussia Dipartimento di Fisica"E.pancini" Universitàdi Napoli Federico ⅡComplesso Universitario di Monte S.AngeloVia CintiaEdificio 680126 NapoliItaly Istituto Nazionale di Fisica Nucleare Sezione di Napoli80126 NapoliItaly University of science 700000 Ho Chi Minh CityVietnam

Radiative decays D((s))*→D((s))γare revisited in light of new experimental data from the BaBar and BEsⅢ*** radiative couplings gD*Dγencoding nonperturbative QcD effects are calculated in the framework of the covariant confined quark model developed by *** compare our results with other theoretical studies and experimental *** couplings(in GeV-1)|g(D*+D+γ)|=0.45(9)and|g(D*0D0γ)|=1.72(34)calculated in our model agree with the corresponding experimental data|g(D*+D+γ)|=0.47(7)and|g(D*0D0γ)|=1.77(16).The most interesting case is the decay Ds*→Dsγ,for which a recent prediction based on light-cone sum rules at next-to-leading order|gDs*Dsγ|=0.60(19)deviates from the first(and only to date)lattice QcD result|gDs*Dsγ|=0.11(2)at nearly3σ.Our calculation yields|gDs*Dsγ|=0.29(6),which falls somehow between the two mentioned results,although it is larger than those predicted in other studies using quark models or QcD sum rules.

关键词： covariant quark model radiative decay charmed meson

enhanced entropy generation and heat transfer characteristics of magnetic nano-encapsulated phase change materials in latent heat thermal energy storage systems

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Applied Mathematics and Mechanics(english Edition) 2024年第6期45卷 1051-1070页

作者： p.s.REDDY p.sREEDEVI Department of Mathematics Rajeev Gandhi Memorial College of Engineering and TechnologyNandyal 518501India

The objective of the current study is to investigate the importance of entropy generation and thermal radiation on the patterns of velocity,isentropic lines,and temperature contours within a thermal energy storage device filled with magnetic nanoencapsulated phase change materials(NEpcMs).The versatile finite element method(FEM)is implemented to numerically solve the governing *** effects of various parameters,including the viscosity parameter,ranging from 1 to 3,the thermal conductivity parameter,ranging from 1 to 3,the Rayleigh parameter,ranging from 102 to 3×10^(2),the radiation number,ranging from 0.1 to 0.5,the fusion temperature,ranging from 1.0 to 1.2,the volume fraction of NEpcMs,ranging from 2%to 6%,the stefan number,ranging from 1 to 5,the magnetic number,ranging from 0.1 to 0.5,and the irreversibility parameter,ranging from 0.1 to 0.5,are examined in detail on the temperature contours,isentropic lines,heat capacity ratio,and velocity ***,the heat transfer rates at both the cold and hot walls are analyzed,and the findings are presented *** results indicate that the time taken by the NEpcMs to transition from solid to liquid is prolonged inside the chamber region as the fusion temperatureθf ***,the contours of the heat capacity ratio cr decrease with the increase in the stefan number ste.

关键词： volume fraction of nano-encapsulated phase change material(NEpcM) radiation stefan number heat capacity ratio entropy generation

Effect of radiative loss mechanisms on FIR thermometric parameters of Nd^(3+)-doped lithium tellurite glasses

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Journal of Rare Earths 2024年第7期42卷 1250-1257,I0002页

作者： c.Y.Morassuti A.p.L silva L.A.O.Nunes s.M.Lima L.H.c.Andrade Optical and photothermal spectroscopy Group(GEOF) Center for Studies in Natural Resources(CERNA)State University of Mato Grosso do Sul(UEMS)C.P.351DouradosMSBrazil Renato Archer Information Technology center Dom Pedro I Highway(SP-65)Km 1436-Chácaras Campos dos AmaraisCampinasSPBrazil Institute of physics of são carlos University of São PauloSão CarlosSPBrazil

Nd^(3+)-doped tellurite glasses are promising materials for thermometers based on the fluorescence intensity ratio(FIR)***,at high Nd^(3+)concentrations,energy transfer(ET)processes such as optical reabsorption and cross-relaxation can affect the Nd^(3+)emission,which has been little explored in the ***,the present work investigated the use of Nd^(3+)-doped tellurite glass(samples doped with Nd^(3+)at 0.2 mol%,0.5 mol%,2.0 mol%,and 4.0 mol%)in fluorescence thermometers,in the temperature range from 299 to 371 *** results indicate a strong dependence of the FIR parameters on the Nd^(3+)concentration,due to changes in the emission band profiles caused by optical reabsorption of the Nd^(3+)emissions and cross-relaxation processes.A decrease of the relative sensitivity of the ratio^(4)F_(5/2)→^(4)I_(9/2)/^(4)F_(3/2)→^(4)I_(9/2)is observed for samples doped with higher amounts of Nd^(3+).The maximum relative sensitivity at 299 K is 3.00%/K,which is the highest value among the reported Nd^(3+)ions.

关键词： Nd^(3+)-doped tellurite glass Fluorescence intensity ratio Optical thermometry Judd-Ofelt Rare earths

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Transcriptome analysis suggests broad jejunal alterations in Linghu’s obesity-diarrhea syndrome:A pilot study

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World Journal of Gastroenterology 2024年第21期30卷 2777-2792页

作者： Xiao-Tong Niu Xiang-Yao Wang Yan Wang Ke Han Nan Ru Jing-Yuan Xiang en-Qiang Linghu Medical school of chinese pLA Chinese PLA General HospitalBeijing 100853China Department of Gastroenterology The First Medical Center of Chinese PLA General HospitalBeijing 100853China school of Medicine Nankai UniversityTianjin 300071China

BAcKGROUND Obesity is associated with a significantly increased risk for chronic diarrhea,which has been proposed as Linghu’s obesity-diarrhea syndrome(ODs);however,its molecular mechanisms are largely *** To reveal the transcriptomic changes in the jejunum involved in *** In a cohort of 6 ODs patients(JOD group),6 obese people without diarrhea(JO group),and 6 healthy controls(Jc group),high-throughput sequencing and bioinformatics analyses were performed to identify jejunal mucosal mRNA expression alterations and dysfunctional biological *** another cohort of 16 ODs patients(sOD group),16 obese people without diarrhea(sO group),and 16 healthy controls(sc group),serum diamine oxidase(DAO)and D-lactate(DLA)concentrations were detected to assess changes in intestinal barrier *** The gene expression profiles of jejunal mucosa in the JO and Jc groups were similar,with only 1 differentially expressed gene(DEG).The gene expression profile of the JOD group was significantly changed,with 411 DEGs compared with the JO group and 211 DEGs compared with the Jc group,129 of which *** enrichment analysis of these DEGs showed that the biological processes such as digestion,absorption,and transport of nutrients(especially lipids)tended to be up-regulated in the JOD group,while the biological processes such as rRNA processing,mitochondrial translation,antimicrobial humoral response,DNA replication,and DNA repair tended to be down-regulated in the JOD *** DEGs(cDT1,NHp2,EXOsc5,EpN3,NME1,REG3A,pLA2G2A,and pRss2)may play a key regulatory role in the pathological process of ODs,and their expression levels were significantly decreased in ODs patients(psecond cohort,compared with healthy controls,the levels of serum intestinal barrier function markers(DAO and D-LA)were significantly increased in all obese individuals(psOD group than in the sO group(pcONcLUsION compared with health

关键词： Obesity chronic diarrhea Jejunum Transcriptome Intestinal barrier

seasonal changes in Hermit Thrush (catharus guttatus) song syntax

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Avian Research 2024年第2期15卷 172-179页

作者： sean p.Roach Luke c.McLean Jeff W.MacLeod Leslie s.phillmore psychology Department University of New Brunswick Saint John100 Tucker Park RoadSaint JohnNBCanada Department of psychology and Neuroscience Dalhousie University1355 Oxford StHalifaxNSB3H 4J1Canada

In many songbird species,birdsong features phonological syntax,meaning that the units within their vocal se-quences are ordered in a non-random way that adheres to a *** such syntactical patterns have been richly described in many species,comparatively little is known about how those patterns contribute to song achieving its important *** each of song’s main functions,territorial defense and mate attraction,evidence of a role for syntax is *** species for which syntax has been thoroughly described is the Hermit Thrush(catharus guttatus),which presents song types from their repertoires in a semi-predictable order and,in doing so,rapidly cycle up and down the frequency *** objective of the present study was to explore the importance of song syntax in the Hermit Thrush through a within-subject examination of how measures of syntax,such as the predictability of song type order within song sequences,shift over the breeding *** hypothesized that,if such syntactical characteristics are important to breeding behaviour,they would be most prominent at the start of the breeding season when activity associated with territory establishment and mate attraction is most *** revealed that,as predicted,the rigidness of song type ordering within se-quences was highest at the start of the season and declined *** song type sequences were most predictable at the vitally important early part of the breeding season fit our hypothesis that this aspect of song syntax is important to song’s functions related to territory establishment and/or mate *** work will clarify whether that role relates to one of song’s two main functions or serves song transmission in some broader way.

关键词： Birdsong Hermit Thrush seasonal change stereotypy syntax

Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells

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Regenerative Biomaterials 2024年第2期3卷 76-87页

作者： Tao Lu Long Yang Zhuoyang Li Yin Liu shun'en Xu chuan Ye Department of Orthopaedics The Affliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Orthopaedics The First People's Hospital of GuiyangGuiyang 550004China center for Tissue engineering and stem cell Research Guizhou Medical UniversityGuiyang 550004China Department of Otolaryngology Head and Neck surgery Xinqiao HospitalArmy Medical University(Third Military Medical University)Chongqing 400037China Department of Dental implant Stomatological Hospital of GuiyangGuiyang 550000China

This study presents the development and evaluation of a poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(p34HB)ultrafine fiber slow-release system for in vivo osteogenic induction of human umbilical cord mesenchymal stem cells(HUcMscs).Utilizing dual-nozzle and cell electrospinning techniques,the system encapsulates L-ascorbic acid-2-phosphate magnesium(Asp),βB-glycerophosphate sodium and dexamethasone(DEX)within the'fbers,ensuring sustained osteogenic *** scaffold's morphology,characterization,hydrophilicity,mechanical properties and cellular behavior were *** subcutaneous implantation in rabbits was con-ducted to observe its ectopic osteogenic induction *** fabricated p34HB ultrafine fiber slow-release *** ization *** distribution of HUcMscs and inducing components within the scaffold,with no chemical reactions affecting the active *** vitro tests showcased a prolonged release of DEX and Asp,while biocompatibility assays highlighted the scaffold's suitability for cellular *** Red,type I collagen,and osteopontin(OpN)staining verifed the scaffold's potent osteogenic induction effect on ***,immediate implantation into New Zealand White rabbits led to significant new bone formation within 8 *** findings underscore the system's potential for immediate in vivo implantation without prior in vitro induction,marking a promising advancement in bone tissue engineering.

关键词： bone tissue engineering cell electrospinning in vivo induction immediate implantation biomaterials

Molecular mechanism of Xuebijing in treating pyogenic liver abscess complicated with sepsis

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World Journal of Emergency Medicine 2024年第1期15卷 35-40页

作者： Wei Zhou Maiying Fan Xiang Li Fang Yu en Zhou Xiaotong Han Department of Emergency Medicine the First Affi liated Hospital of Hunan Normal University/Hunan Provincial People’s HospitalChangsha 410005China Department of Otolaryngology-head and Neck surgery the First Affi liated Hospital of Hunan Normal University/Hunan Provincial People’s HospitalChangsha 410005China

BAcKGROUND:Xuebijing(XBJ)can alleviate the inflammatory response,improve organ function,and shorten the intensive care unit(IcU)stay in patients with pyogenic liver abscess(pLA)complicated with sepsis,but the molecular mechanisms have not been *** study aimed to explore the molecular mechanism of XBJ in treating pLA complicated with sepsis using a network pharmacology ***:The active ingredients and targets of XBJ were retrieved from the ETcM *** targets related to pLA and sepsis were retrieved from the Genecards,pharmGKB,DisGenet,Online Mendelian Inheritance in Man(OMIM),Therapeutic Targets Database(TTD),and DrugBank *** targets of pLA complicated with sepsis were mapped to the targets of XBJ to identify potential treatment ***-protein interaction networks were analyzed using the sTRING *** treatment targets were imported into the Metascape platform for Gene Ontology(GO)functional enrichment and Kyoto encyclopedia of Genes and Genomes(KEGG)pathway enrichment *** docking was performed to validate the interactions between active ingredients and core ***:XBJ was found to have 54 potential treatment targets for pLA complicated with ***-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor(TNF)were identifi ed as core *** enrichment analysis revealed important pathways,including the interleukin-17(IL-17)signaling pathway,the TNF signaling pathway,the nuclear factor-kappa B(NF-κB)signaling pathway,and the Toll-like receptor(TLR)signaling *** docking experiments indicated stable binding between XBJ active ingredients and core ***:XBJ may exert therapeutic eff ects on pLA complicated with sepsis by modulating signaling pathways,such as the IL-17,TNF,NF-κB,and TLR pathways,and targeting IL-1β,IL-6,and TNF.

关键词： Xuebijing injection pyogenic liver abscess sepsis

Geochemistry and depositional environment of fuchsite quartzites from sargur Group,western Dharwar craton,India

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Acta Geochimica 2024年第3期43卷 555-570页

作者： c.s.sindhuja G.Harshitha c.Manikyamba K.s.V.subramanyam csIR(council of scientific and Industrial Research)-National Geophysical Research Institute(NGRI) Uppal RoadHyderabad 500007India

Meso-Neoarchean fuchsite quartzites are present in different stratigraphic positions of Dharwar craton including the oldest(~3.3 Ga)sargur Group of western Dharwar *** present study deals with the petro-graphic and geochemical characteristics of the fuchsite quartzites from the Ghattihosahalli belt to evaluate their genesis,depositional setting and the enigma involved in the ancient sedimentation *** major mineral assemblages include quartz,fuchsite,and feldspars along with accessory kyanite and *** geochemical com-positions are characterized by high siO_(2),Al_(2)O_(3),low MgO,caO,strongly enriched cr(1326–6899 ppm),Ba(1165–3653 ppm),sr(46–210 ppm),V(107–868 ppm)and Zn(11–158 ppm)contents compared to the upper continental crust(Ucc).The Ucc normalized rare earth element(REE)patterns are characterized by depleted light REE[(La/sm)Ucc=0.33–0.95]compared to heavy REE[(Gd/Yb)_(Ucc)=0.42–1.65]with conspicuous positive Eu-anomalies(Eu/Eu^(*)=1.35–18.27)characteristic of hydrothermal solutions evidenced through the interlayered *** overall major and trace element systematics reflect a combined mafic-felsic provenance and suggest their deposition at a passive continental margin *** comprehensivefield,petrographic,and geo-chemical studies indicate that these quartzites are infiltrated by cr-richfluids released during high-grade metamorphism of associated ultramafic *** sargur and the subse-quent Dharwar orogeny amalgamated diverse lithounits from different tectonic settings,possibly leading to the release of cr-richfluids and the formation of fuchsite quartzite during or after the *** sug-gest a pre-existing stable crust prior to the sargur Group and the link between orogenic events and various mineral deposits in the Dharwar craton.

关键词： Dharwar craton Ghattihosahalli Fuchsite quartzite provenance Depositional setting