Gapless Genome Assembly of ZH8015 and Preliminary Multi-Omics Analysis to Investigate ZH8015's Responses Against Brown Planthopper Infestation

作     者：LI Dian DUAN Wenjing LIU Qun’en WU Weixun ZHAN Xiaodeng SUN Lianping ZHANG Yingxin CHENG Shihua LI Dian;DUAN Wenjing;LIU Qun'en;WU Weixun;ZHAN Xiaodeng;SUN Lianping;ZHANG Yingxin;CHENG Shihua

作者机构：State Key Laboratory of Rice Biology and BreedingChina National Rice Research InstituteHangzhou 310006China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2024年第31卷第3期

页      面：317-327,I0042-I0045页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the Chinese Academy of Agricultural Sciences Innovation Project(Grant No.CAASASTIP-2013CNRRI) Fundamental Research Funds for Central Public Welfare Research Institutes of Chinese Rice Research Institute(Grant No.CPSIBRF-CNRRI-202102) 

主　　题：brown planthopper gapless genome genome assembly multi-omics Nilaparvata lugens rice 

摘      要：Accurate genomic information is essential for advancing genetic breeding research in specific rice *** study presented a gapless genome assembly of the indica rice cultivar Zhonghui 8015(ZH8015)using Pac Bio HiFi,Hi-C,and ONT(Oxford Nanopore Technologies)ultra-long sequencing technologies,annotating 43037 gene ***,utilizing this genome along with transcriptomic and metabolomic techniques,we explored ZH8015 s response to brown planthopper(BPH)*** transcriptomic sampling indicated significant changes in gene expression levels around 48 h after BPH *** analysis revealed particularly significant alterations in genes related to reactive oxygen species scavenging and cell wall *** results demonstrated marked increases in levels of several monosaccharides,which are components of the cell wall and dramatic changes in flavonoid *** association analysis identified differentially expressed genes associated with key metabolites,shedding light on ZH8015 s response to BPH *** summary,this study constructed a reliable genome sequence resource for ZH8015,and the preliminary multi-omics results will guide future insect-resistant breeding research.