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Single CD271 marker isolates mesenchymal stem cells from human dental pulp

International Journal of Oral Science 2015年第4期7卷 205-212页

作者： ruth alvarez Hye-Lim Lee Christine Hong Cun-Yu Wang Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles Department of Bioengineering Henry Samueli School of Engineering and Applied ScienceUniversity of California at Los Angeles

Mesenchymal stem cells （MSCs） are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow （BMSCs）, adult MSCs are isolated from craniofacial tissues including dental pulp tissues （DPs） using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cel^s （~M^Cs）. II1 ~his stucly, we used clif^et（~nt combinations of surface markers （CD51/CD140a, CD271, and STRO-1/CD146） to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells （DPCs） obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51＋/CD140a＋, 10.6% were CD271＋, and 0.3% were STRO-1＋/CD146＋. Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271＋ DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271＋ DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.

关键词： dental mesenchymal stem cells odontogenic differentiation cell surface markers dental pulp fluorescence-activated cellsorting

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Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers

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International Journal of Oral Science 2015年第4期7卷 213-219页

作者： ruth alvarez Hye-Lim Lee Cun-Yu Wang Christine Hong Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles

Mesenchymal stem cell （MSC）-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations： CD51/CD140a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells （DMSCs） from heterogeneous periodontal ligament cells （PDLCs）. Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51＋/CD140a＋, 0.8% were CD271＋, and 2.4% were STRO-1＋/CD146＋. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271＋ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.

关键词： cell surface markers dental mesenchymal stem cells periodontal ligament

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Hydroxytyrosol and Parkinson's disease:protective actions against alpha-synuclein toxicity

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Neural Regeneration Research 2024年第7期19卷 1427-1428页

作者： ruth Hornedo-Ortega Ana M.Espinosa-Oliva Departamento de Nutrición y Bromatología Toxicología y Medicina LegalFacultad de FarmaciaUniversidad de SevillaSevilleSpain Instituto de Biomedicina de Sevilla IBiS/Hospital Universitario Virgen del Rocío/CSIC/Universidad de SevillaSevillaSpain Departamento de Bioquímica y Biología Molecular Facultad de FarmaciaUniversidad de SevillaSevillaSpain

Parkinson’s disease:Parkinson’s disease(PD) is the second most prevalent neurodegenerative disease,after Alzheimer’s disease,affecting 1%of the general population over the age of *** to data from the World Health Organization(WHO),its prevalence has doubled in the past 25 *** 2019,global estimates indicated over 8.5 million individuals with PD and it is suggested that PD caused 329000 deaths,an increase of over 100% since 2000(WHO,2022).

关键词： protective Alzheimer prevalence

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Consequences of early life stress on the structure and function of the adult mouse retina

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Neural Regeneration Research 2024年第1期19卷 150-151页

作者： Juan S.Calanni ruth E.Rosenstein Laboratory of Retinal Neurochemistry and Experimental Ophthalmology Department of Human BiochemistrySchool of Medicine/CEFyBOUniversity of Buenos Aires/CONICETBuenos AiresArgentina

Early-life stress(ELS)refers to a period of environmental/social deprivation,physica l,sexual or emotional abuse,neglect,severe and/or chronic trauma in the prenatal/early postnatal stage,which is closely related to many adverse psychiatric disorders later in life,such as depressive disorder,substance abuse,dysthymia,panic,anxiety,and suicidal behavior in adulthood(Waters and Gould,2022).

关键词： stress life Gould

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Inferring community transmission of SARS-CoV-2 in the United Kingdom using the ONS COVID-19 Infection Survey

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Infectious Disease Modelling 2024年第2期9卷 299-313页

作者： ruth McCabe Gabriel Danelian Jasmina Panovska-Griffiths Christl A.Donnelly Department of Statistics University of OxfordUK National Institute for Health and Care Research Health Protection Research Unit in Emerging and Zoonotic Infections UK United Kingdom Health Security Agency UK The Queen's College University of OxfordUK The Pandemic Sciences Institute University of OxfordUK MRC Centre for Global Infectious Disease Analysis Imperial College LondonUK

Key epidemiological parameters,including the effective reproduction number,,and the instantaneous growth rate,,generated from an ensemble of models,have been informing public health policy throughout the COVID-19 pandemic in the four nations of the United Kingdom of Great Britain and Northern Ireland(UK).However,estimation of these quantities became challenging with the scaling down of surveillance systems as part of the transition from the“emergency”to“endemic”phase of the *** Office for National Statistics(ONS)COVID-19 Infection Survey(CIS)provided an opportunity to continue estimating these parameters in the absence of other data *** used a penalised spline model fitted to the publicly-available ONS CIS test positivity estimates to produce a smoothed estimate of the prevalence of SARS-CoV-2 positivity over *** resulting fitted curve was used to estimate the“ONS-based”and across the four nations of the *** produced under this model are compared to government-published estimates with particular consideration given to the contribution that this single data stream can offer in the estimation of these *** on the nation and parameter,we found that up to 77%of the variance in the government-published estimates can be explained by the ONS-based estimates,demonstrating the value of this singular data stream to track the epidemic in each of the four *** additionally find that the ONS-based estimates uncover epidemic trends earlier than the corresponding government-published *** work shows that the ONS CIS can be used to generate key COVID-19 epidemiological parameters across the four UK nations,further underlining the enormous value of such population-level studies of *** is not intended as an alternative to ensemble modelling,rather it is intended as a potential solution to the aforementioned challenge faced by public health officials in the UK in early 2022.

关键词： Effective reproduction number Instantaneous growth rate SARS-CoV-2 COVID-19 ONS COVID-19 Infection Survey Surveillance

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Reducing Food Waste

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空中英语教室（初级版．大家说英语） 2024年第11期 30-33,53,54,56页

作者： ruth Devlin 不详

For many people in the world,wasting food is a bad *** buy more than they need at the *** they let fruits and vegetables spoil at *** can you reduce food waste?First,don't buy too *** may not be able to eat it all before it *** careful when you store *** older things in the front of your cupboards or *** use those things *** you cook a meal and don't finish it,freeze it for ***'t throw it away.

关键词： store things finish

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It's an Emergency!

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空中英语教室（初级版．大家说英语） 2024年第11期 38-41,55,56页

作者： ruth Devlin 不详

It's an emergency!It may be a fire or a *** may be hurt or in *** need help in a *** do you do?You can call the emergency *** is 911 in the *** can ask for *** can ask for the police or fire *** can ask for an ambulan... 详细信息

关键词： Someone number. need

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BACK TO SCHOOL

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空中英语教室（初级版．大家说英语） 2024年第9期 9-12,50,51,56页

作者： ruth Devlin 不详

Many students feel excited about going back to *** want to meet new teachers,new classmates and learn new *** somestudents *** school starts,they can't sleep in or text their friends all the *** also worry about homew... 详细信息

关键词： excited worry friends

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Let's Play Ultimate!

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空中英语教室（初级版．大家说英语） 2024年第11期 10-13,50,51,56页

作者： ruth Devlin 不详

Do you like to throw and catch a Frisbee?If so,you might like playing *** is a fast-moving team ***'s often played outside on a *** are two teams of seven players *** pass the Frisbee from player to player up the *** can run and jump until they catch the *** they must plant one *** they throw the Frisbee,they can move again.A team scores a point when a player catches the Frisbee in the end zone.

关键词： field. Player outside

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ONE GRAIN OF RICE

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空中英语教室（初级版．大家说英语） 2024年第9期 20-23,52,56页

作者： ruth Devlin 不详

ong ago,a raja lived in *** people were rice *** raja said to them,"Give me most of your rice,and I will store *** the time of famine comes,everyone will have rice to eat."So each year,the raja took most of the people... 详细信息

关键词： everyone store stored

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