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Establishing cell suitability for high-level production of licorice triterpenoids in yeast

Acta Pharmaceutica Sinica B 2024年第9期14卷 4134-4148页

作者： Wentao Sun Shengtong Wan Chuyan Liu Ruwen wang Haocheng Zhang Lei Qin runming wang Bo Lv Chun Li Department of Chemical Engineering Tsinghua UniversityBeijing 100084China Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Ministry of Industry and Information TechnologyInstitute of Biochemical EngineeringSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China The University of Chicago ChicagoIL 60637USA Key Lab for Industrial Biocatalysis Ministry of EducationTsinghua UniversityBeijing 100084China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China

Yeast has been an indispensable host for synthesizing complex plant-derived naturalcompounds, yet the yields remained largely constrained. This limitation mainly arises from overlookingthe importance of cell and pathway suitability during the optimization of enzymes and pathways. Herein,beyond conventional enzyme engineering, we dissected metabolic suitability with a framework forsimultaneously augmenting cofactors and carbon flux to enhance the biosynthesis of heterogenoustriterpenoids. We further developed phospholipid microenvironment engineering strategies, dramaticallyimproving yeast’s suitability for the high performance of endoplasmic reticulum (ER)-localized, ratelimitingplant P450s. Combining metabolic and microenvironment suitability by manipulating only threegenes, NHMGR (NADH-dependent HMG-CoA reductase), SIP4 (a DNA-binding transcription factor)andGPP1 (Glycerol-1-phosphate phosphohydrolase 1), we enabled the high-level production of 4.92 g/L rarelicorice triterpenoids derived from consecutive oxidation of b-amyrin by two P450 enzymes afterfermentation optimization. This production holds substantial commercial value, highlighting the criticalrole of establishing cell suitability in enhancing triterpenoid biosynthesis and offering a versatileframework applicable to various plant natural product biosynthetic pathways.

关键词： Cell suitability Phospholipid microenvironment Cytochrome P450 Triterpenoids Saccharomyces cerevisiae

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Gut lumen-targeted oral delivery system for bioactive agents to regulate gut microbiome

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Journal of Future Foods 2022年第4期2卷 307-325页

作者： Junyu Liu Xiaobin Li Xiaopeng Zhang runming wang Sanyang Han Peiwu Qin Xin-Hui Xing Canyang Zhang Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biochemical Engineering Department of Chemical EngineeringKey Laboratory for Industrial BiocatalysisMinistry of EducationCenter for Synthetic and Systems BiologyTsinghua UniversityBeijing 100084China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

Gut microbiome(GM)is closely related to the overall health status for the human *** dysfunction of microbiome can lead to many diseases such as inflammation,cancer and neurodegenerative ***,it's of great importance to develop effective strategy to regulate *** lumen-targeted oral delivery system(GLT-ODS)has been extensively investigated and widely used in food science and engineering in these years,due to the targeted delivery property,controlled release profile,high biocompatibility and enhanced bioavailability of ***,we comprehensively summarized the current advances in GLT-ODS for bioactive ***,we systematically summarize the GLT-ODS for pre-agents(prebiotics and probiotics)and anti-agents(antibiotics and bacteriophages)according to the type of *** in-depth discussion of representative researches,we refined the limitations of existing research in carrier material and target selection,and drew a blueprint for the future technological research and *** believe that GLT-ODS will become a safe,efficient,simple and precise GM management strategy for improvement of health and shine in the development of precision food and pharmaceutical engineering in future.

关键词： Gut lumen-targeted Oral delivery systems Bioactive agents Gut microenvironment Food engineering

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Lithiophilic CoF_(2)@C hollow spheres towards spatial lithium deposition for stable lithium metal batteries

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Journal of Energy Chemistry 2024年第10期97卷 55-67,I0002页

作者： Jianxing wang Shuhao Yao runming Tao Xiaolang Liu Jiazhi Geng Chang Hong Huiying Li Guiyun Yu Haifeng Li Xiao-Guang Sun Jianlin Li Jiyuan Liang School of Optoelectronic Materials&Technology jianghan UniversityWuhan 430056HubeiChina Institute for Advanced Materials and Manufacturing University of TennesseeKnoxilTN 37996USA School of Chemistry&Chemical Engineering Yancheng Institute of TechnologyYancheng 224051JiangsuChina Chemical Sciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA Applied Materials Division Argonne National LaboratoryLemontIL 60439USA

Lithium metal(LM)is a promising anode for next-generation batteries due to its high theoretical capacity and low electrode ***,side reactions,volume change,and unwanted lithium dendrite growth seriously limit the practical application of ***,with the aid of a hard template approach,a novel lithiophilic CoF_(2)-carbon hollow sphere(CoF_(2)@C-HS)composite material is successfully prepared via a facile in-situ fluorination and etching *** lithiophilic CoF_(2) acts as nucleation sites to reduce nucleation overpotential as well as induces the spatial Li deposition and the formation of LiFrich solid electrolyte interphase(SEI),and the hollow carbon matrix can enhance the electrical conductivity and offer free space for LM *** simulations reveal that the synergistic effect of lithiophilic CoF_(2) and hollow carbon matrix homogenizes the electric field distribution and Li~+*** from these advantages,the CoF_(2)@C-HS-modified copper substrate electrode delivers an enhanced Coulombic efficiency(CE)of 93.7%for 280 cycles at 1 mA cm^(-2)and 1 mA h cm^(-2).The symmetrical cell using CoF_(2)@C-HS can stably cycle more than 1800 h with a low voltage hysteresis of 11 mV at a current density of 0.5 MA cm^(-2)and an areal capacity of 0.5 mA h cm^(-2).Moreover,the Li@CoF_(2)@C-HS composite anode enables more than 300 stable cycles at 1 C with a capacity retention of 95%in LiFePO_(4)-based full cell and 110 stable cycles at 1 C in LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)-based highvoltage full *** work might shed a new light on designing lithiophilic hosts to spatially confine LM deposition,realizing dendrite-free LM anodes and the practical applications of LM batteries.

关键词： Lithium metal anode Spatial deposition Stability Nanocomposite Lithiophilic CoF_(2)

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Matching metals to proteins in cells: identification of protein targets and discovery of drug candidates

Matching metals to proteins in cells: identification of prot...

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第十一届全国化学生物学学术会议

作者：孙红哲 runming wang Haibo wang Richard Yi-Tsun Kao Pak-Leung Ho Hongyan Li 香港大学 Department of Chemistry The University of Hong Kong Department of Microbiology The University of Hong Kong

＜正＞Metal-and metallodrug-protein interactions play a crucial role for the action of *** is crucial to identify metal-protein interactions at a proteome-wide scale which are difficult due to diversity of metal-protein interactions（1,2）.We developed an integrated approach to identify metal-associated proteins using bismuth,silver and gallium as an example（3,4） as well as to quantify the metals for rapid metallome/proteome-wide profiling of metal-binding proteins.

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From Metallomics to Drug Development: Overcoming Antibiotics Resistance（AMR）

From Metallomics to Drug Development: Overcoming Antibiotics...

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中国化学会第十四届全国生物无机化学学术会议

作者： Hongzhe Sun runming wang Tse-Pui Lai Pak-Leung Ho Patrick Chiu-Yat Woo Richard Yi-Tsun Kao Hongyan Li Department of Chemistry the University of Hong Kong Department of Microbiology the University of Hong Kong

＜正＞At least 25 metals are essential to lives and metal compounds have also long been used in medicine and healthcare. Metal-and metallodrug-protein interactions play a crucial role for metallodrugs. It is critical to identify metal-protein interactions at a proteome-wide scale which are difficult due to diversity of metal-protein interactions（1,2）. We developed an integrative metallomic approach consisting of continuous-flow gel electrophoresis and inductively coupled plasma mass spectrometry,

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高职产业学院的内涵、价值与路径研究

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常州信息职业技术学院学报 2023年第3期22卷 5-9页

作者：王润铭巩有奎烟台职业学院山东烟台264670

产业学院是深化产教融合的创新举措,是实现教育链、人才链与产业链、创新链有效衔接的重要载体。产业学院是知识生产模式转变和社会经济发展的产物,兼具教育性和产业性。通过政行企校四方联动,将高职产业学院打造成校企资源共享体、教... 详细信息

产业学院是深化产教融合的创新举措,是实现教育链、人才链与产业链、创新链有效衔接的重要载体。产业学院是知识生产模式转变和社会经济发展的产物,兼具教育性和产业性。通过政行企校四方联动,将高职产业学院打造成校企资源共享体、教育教学共同体、生产实训共生体、培训服务联合体、科技创新综合体,实现“产、学、研、用、创、培、服”七位一体的社会功能,以产业学院的建设促进职业教育和区域经济的高质量发展,推动产教融合型城市的建设和共同富裕的实现。

关键词：产业学院内涵价值路径

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基于Walsh特征的快速人脸检测方法

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微型机与应用 2011年第21期30卷 34-36,39页

作者：张剑李坤王润明湖南科技大学信息与电气工程学院湖南湘潭411100

通过构造Walsh变换矩阵,得到相互正交且形状丰富的Walsh特征算子,将Walsh特征应用于快速人脸检测。Matlab的仿真实验结果表明,由Walsh特征得到的强分类器比传统的Haar特征得到的强分类器分类速度快,精度高。

关键词： Walsh特征 Haar特征强分类器人脸检测

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Urban heat mitigation by green and blue infrastructure:Drivers,effectiveness,and future needs

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The Innovation 2024年第2期5卷 145-166页

作者： Prashant Kumar Sisay E.Debele Soheila Khalili Christos H.Halios Jeetendra Sahani Nasrin Aghamohammadi Maria de Fatima Andrade Maria Athanassiadou Kamaldeep Bhui Nerea Calvillo Shi-Jie Cao Frederic Coulon Jill L.Edmondson David Fletcher Edmilson Dias de Freitas Hai Guo Matthew C.Hort Madhusudan Katti Thomas Rodding Kjeldsen Steffen Lehmann Giuliano Maselli Locosselli Shelagh K.Malham Lidia Morawska Rajan Parajuli Christopher D.F.Rogers runming Yao Fang wang Jannis Wenk Laurence Jones Global Centre for Clean Air Research(GCARE) School of SustainabilityCivil and Environmental EngineeringFaculty of Engineering and Physical SciencesUniversity of SurreyGuildford GU27XHUK Institute for Sustainability University of SurreyGuildford GU27XHSurreyUK School of Built Environment University of ReadingWhiteknightsReading RG66BUUK School Design and the Built Environment Curtin University Sustainability Policy InstituteKent StBentley 6102Western Australia Harry Butler Institute Murdoch UniversityMurdoch 6150Western Australia Atmospheric Sciences Department Institute of AstronomyGeophysics and Atmospheric SciencesUniversity of Sao PauloSao Paulo 05508-090Brazil Met Office FitzRoy RoadExeter EX13PBUK Department of Psychiatry and Nuffield Department of Primary Care Health Sciences Wadham CollegeUniversity of OxfordOxfordUK Centre for Interdisciplinary Methodologies University of WarwickWarwickUK School of Architecture Southeast University2 SipailouNanjing 210096China Cranfield University School of WaterEnvironment and EnergyCranfield MK430ALUK Plants PhotosynthesisSoil ClusterSchool of BiosciencesUniversity of SheffieldSheffield S102TNUK UK Centre for Ecology&Hydrology Environment Centre WalesDeiniol RoadBangor LL572UWUK Air Quality Studies Department of Civil and Environmental EngineeringThe Hong Kong Polytechnic UniversityHong KongChina Department of Forestry and Environmental Resources Faculty Excellence Program for Leadership in Public ScienceNorth Carolina State UniversityChancellorRaleighNC 27695USA Departments of Architecture&Civil Engineering and Chemical EngineeringUniversity of BathBath BA27AYUK School of Architecture University of NevadaLas VegasNV 89154USA Department of Tropical Ecosystems Functioning Center of Nuclear Energy in AgricultureUniversity of São PauloPiracicaba 13416-000Sao PauloBrazil School of Ocean Sciences Bangor UniversityMenai BridgeAnglesey LL595 ABUK International Laboratory for Air Quality and Health Science and Engine

The combination of urbanization and global warming leads to urban overheating and compounds the frequency and intensity of extreme heat events due to climate ***,the risk of urban overheating can be mitigated by urban green-blue-grey infrastructure(GBGI),such as parks,wetlands,and engineered greening,which have the potential to effectively reduce summer air *** many reviews,the evidence bases on quantified GBGI cooling benefits remains partial and the practical recommendations for implementation are *** systematic literature review synthesizes the evidence base for heat mitigation and related co-benefits,identifies knowledge gaps,and proposes recommendations for their implementation to maximize their *** screening 27,486 papers,202 were reviewed,based on 51 GBGI types categorized under 10 main *** GBGI(green walls,parks,street trees)have been well researched for their urban cooling ***,several other GBGI have received negligible(zoological garden,golf course,estuary)or minimal(private garden,allotment)*** most efficient air cooling was observed in botanical gardens(5.0±3.5℃),wetlands(4.9±3.2℃),green walls(4.1±4.2℃),street trees(3.8±3.1℃),and vegetated balconies(3.8±2.7℃).Under changing climate conditions(2070–2100)with consideration of RCP8.5,there is a shift in climate subtypes,either within the same climate zone(e.g.,Dfa to Dfb and Cfb to Cfa)or across other climate zones(e.g.,Dfb[continental warm-summer humid]to BSk[dry,cold semi-arid]and Cwa[temperate]to Am[tropical]).These shifts may result in lower efficiency for the current GBGI in the *** the importance of multiple services,it is crucial to balance their functionality,cooling performance,and other related co-benefits when planning for the future *** global GBGI heat mitigation inventory can assist policymakers and urban planners in prioritizing effective interventions to reduce the risk of urban overheating,fi

关键词： estuary walls extreme

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山西省、重庆市4县区农村居民身体活动状况研究

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中华流行病学杂志 2018年第3期39卷 302-307页

作者：赵一凡马吉祥李剑虹陈波阳扬梁好王丽洁彭代彬金明贵王爱民李润明李万华王文绢中国疾病预防控制中心慢性非传染性疾病预防控制中心北京100050 山西省疾病预防控制中心慢性病防控科太原030012 重庆市大足区疾病预防控制中心 402360 重庆市綦江区疾病预防控制中心 401420 山西省寿阳县疾病预防控制中心慢性病防控科 045400 重庆市大足区疾病预防控制中心慢性病防控科 402360

目的了解山西、重庆农村地区居民身体活动状况及影响因素。方法选取山西、重庆4县区当地居住≥1年，且2年内没有迁出计划的≥18岁居民为调查对象。通过面对面问卷调查，收集调查对象每日身体活动时间，静坐时间，相关知识、态度情况等... 详细信息

目的了解山西、重庆农村地区居民身体活动状况及影响因素。方法选取山西、重庆4县区当地居住≥1年，且2年内没有迁出计划的≥18岁居民为调查对象。通过面对面问卷调查，收集调查对象每日身体活动时间，静坐时间，相关知识、态度情况等数据。结果调查对象身体活动不足率为14．9％，从不锻炼率为88．7％，每日静坐时间（3．91±2．06）h，多因素分析结果显示文化程度、人均月收入和身体活动行为阶段是身体活动不足的影响因素。结论调查地区从不锻炼率高于全国农村平均水平，应采取措施全面提高锻炼率。对“有锻炼意愿、但无行动”人群和已增加身体活动的人群，在加强健康教育的同时，应有针对性地提供指导。

关键词：身体活动农村横断面调查

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基于LabWindows/CVI的光电三极管虚拟测试仪设计

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计算机测量与控制 2016年第5期24卷 287-290页

作者：胡锦超赵祥模王润民杨楠徐江长安大学信息工程学院西安710064

光电三极管具有伏安,光照,温度,功率等多种特性,正确选择使用某种型号的光电三极管之前需要熟悉其特性,因此需要一种便携、廉价、易操作的测试仪对光电三极管的特性进行测试,文章设计出基于LabWindows/CVI设计了一种光电三极管虚拟测试... 详细信息

光电三极管具有伏安,光照,温度,功率等多种特性,正确选择使用某种型号的光电三极管之前需要熟悉其特性,因此需要一种便携、廉价、易操作的测试仪对光电三极管的特性进行测试,文章设计出基于LabWindows/CVI设计了一种光电三极管虚拟测试仪,用高速采集卡和LabWindows/CVI取代传统的模拟电子电路测试仪器完成光电三极管特性的测量;在构建虚拟仪器测试方案的基础上,首先设计了一种信号放大电路用于光电三极管输出信号的放大处理,然后提出了光电三极管特性测量方法,最后阐述了labWindows/CVI环境下的光电三极管测试数据处理过程;实验测试表明:所设计的虚拟测试仪能够准确地对光电三极管的伏安特性和光照特性进行测量,测试结果形象化地显示在仪器面板上,有效地避免因工作时参数过高对光电三极管造成的影响。

关键词：虚拟测试仪特性测试光电三极管 LabWindows/CVI

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