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S波段和X波段微波单一和复合暴露致雄性大鼠生殖损伤差异及机制研究

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军事医学 2024年第8期48卷 565-571页

作者：李艳阳庞悦悦赵黎王浩宇门俊琦姚斌伟彭瑞云南华大学衡阳医学院军事医学研究院研究生协作培养基地军事科学院军事医学研究院

目的研究2.856和9.375 GHz微波单一和复合暴露对雄性大鼠生殖器官的损伤差异和机制。方法 40只雄性Wistar大鼠随机分为sham组、S10组、X10组和SX5组。S10组和X10组分别采用2.856和9.375 GHz微波平均功率密度10 mW/cm2暴露大鼠6 min,SX... 详细信息

目的研究2.856和9.375 GHz微波单一和复合暴露对雄性大鼠生殖器官的损伤差异和机制。方法 40只雄性Wistar大鼠随机分为sham组、S10组、X10组和SX5组。S10组和X10组分别采用2.856和9.375 GHz微波平均功率密度10 mW/cm2暴露大鼠6 min,SX5组采用2.856和9.375 GHz微波平均功率密度5 mW/cm2先后暴露大鼠6 min。暴露后1和7 d检测精子活力、血清性激素，光镜和电镜观察睾丸组织结构及检测氧化应激和能量代谢水平。结果 S10组和X10组暴露后1和7 d，精子活力、睾酮（T）、促卵泡生成激素（FSH）和抑制素B（INHB）下降（P<0.01）;SX5组暴露后7 d，精子活力、T、FSH和INHB下降（P<0.05）；各暴露组促黄体生成素激素（LH）暴露后1 d下降（P<0.01）、S10组和X10组暴露后7 d上升（P<0.05）。睾丸组织生精上皮疏松、生精细胞水肿空化，染色质凝集边移，S10组、X10组较SX5组损伤显著。S10组暴露后1和7 d，睾丸组织超氧化物歧化酶（SOD）活性下降、脂质过氧化物丙二醛（MDA）含量升高（P<0.01）;X10组暴露后1 d,SOD下降（P<0.01）。S10组和X10组暴露后1和7 d，睾丸组织乳酸脱氢酶（LDH）、琥珀酸脱氢酶（SDH）活性和三磷酸腺苷（ATP）含量下降（P<0.05）;SX5组暴露后1 d,LDH和SDH下降（P<0.05）。结论 S波段及X波段微波单一和复合暴露均可致雄性生殖器官损伤；S波段较X波段损伤显著；单频微波高强度暴露比多频微波长时间复合暴露损伤显著；该损伤与氧化应激和能量代谢密切相关。

关键词：微波雄性生殖复合暴露氧化应激能量代谢

A novel microcurrent dressing for wound healing in a rat skin defect model

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Military Medical Research 2020年第2期7卷 190-198页

作者： Chao Yu Zhi-Xiu Xu Yan-Hui Hao Ya-Bing Gao Bin-Wei Yao Jing Zhang Bing Wang Zong-Qian Hu ruiyun peng Department of Experimental Pathology Beijing Institute of Radiation MedicineBeijing 100850China department of Internal Neurology The First Affiliated Hospital/Xinxiang Medical UniversityXinxiang 453100HenanChina department of Biotechnology Beijing Institute of Radiation MedicineBeijing 100850China

Background:The exogenous application of low-intensity electric stimulation(ES)may mimic a natural endogenous bioelectric current and accelerate the repair process of skin *** study designed a novel microcurrent dressing(MCD)and evaluated its potential effects on wound healing in a rat skin defect ***:First,wireless ES was integrated into a medical cotton cushion to fabricate the MCD,and its electrical property was examined by using a universal power ***,animal experiments were conducted to evaluate the MCD’s ***-five rats were randomized into control(Con)group,Vaseline gauze(VG)group and MCD group.A full-thickness round skin incision 1.5 cm in diameter was made on the back of each *** from routine disinfection,the Con rats were untreated,whereas the other two groups were treated with VG or *** days 3,7 and 14 post injury,the wound areas were observed and measured using image analysis software following photography,and the skin samples were harvested from wound ***,histopathological morphology was observed routinely by hematoxylin and eosin(HE)staining;tumor necrosis factorα(TNF-α)and interleukin(IL)-1βexpression were detected by Western *** endothelial growth factor(VEGF)and epidermal growth factor(EGF)expression were detected with ***:The MCD generated a sf electric potential greater than 0.95 *** experiments showed that the woundhealing rate in the MCD group was significantly increased compared with the Con and VG groups(P<0.05 or P<0.01).Histopathological observation revealed an alleviated inflammatory response,induced vascular proliferation and accelerated epithelization in the MCD ***,samples from the MCD group expressed reduced TNF-αand IL-1βlevels and increased VEGF and EGF levels compared with those of the other two groups(P<0.05 or P<0.01).However,no significant difference was noted between the Con and VG groups at each time ***:The MCD

关键词： Microcurrent dressing Electric stimulation Skin Wound healing

Hepatitis C and B Virus Infection in Chinese Patients with Extrahepatic Bile Duct Carcinoma

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The Chinese-German Journal of Clinical Oncology 2002年第1期1卷 21-27页

作者： CHENMingyi HUANGZhiqiang CHENLezhen GAOYabing pengruiyun WANGDewen DepartmentofHepatobiliarySurgery GeneralSurgicalResearchInstitutePLAGeneralHospitalBeijing100853China DepartmentofPathology PLAGeneralHospitalBeijing100853China ExperimentalPathologicLaboratory InstituteofMedicalRadiologyAcademyofMilitaryMedicalSciencesBeijing100850China

Objective In China, the incidence of extrahepatic bile duct carcinoma (EBDC) tends to increase over the past decades. The etiology of the noted increase in EBDC is not identified. Approximately, in a half of the overall Chinese patients with EBDC, the causative factors in the development of EBDC have not been *** is a high prevalence of hepatitis C virus (HCV) or hepatitis B virus (HBV) in China, both of which can induce malignant transformation of infected cells and strongly associated with hepatocellular carcinoma (HCC). In this study, EBDC tissues from Chinese patients were examined for the presence of HCV and HBV infection to investigate further the potential causes of EBDC.Methods HCV NS5 protein and HBsAg were detected by labeled streptavidin biotin (LSAB) method; HCV RNA and HBV DNA were detected by in situ polymerase chain reaction (IS-PCR) in formalin fixed, paraffin embedded specimens from 51 Chinese patients with EBDC. HCV RNA and HBV DNA were detected by IS-PCR in 34 Chinese patients with specimens of benign lesions of hepatobiliary tract (control group) .Results In 51 case tissue sections of EBDC, NS5 protein was detected in 14 (27.5%), and HBsAg in 5 (9.8%), HCV RNA in 18 (35.4%) and HBV DNA in 8 (15.9%) .respectively, of which HCV and HBV co-infection was detected in 2 (3.9%). In 34 case tissue sections of the control group, HCV RNA was detected in 2 (5.9 % ), and HBV DNA in 3 (8.8%).Conclusion In this study using standard histochemical and PCR techniques, HCV and HBV genomes and their encoding proteins were detected in the tissues of EBDC. The data show that there is a higher than expected incidence of HCV and HBV presence in EBDC tissues than would be expected on serologic grounds. The detectable rate of HCV RNA in EBDC tissues was significantly higher than in control group (x2 = 9.808, P = 0.002). As a result, this study indicates that there is a correlation between the presence of HCV infection and E

关键词： extrahepatic bile duct carcinoma hepatitis C virus hepatitis B virus in situ polymerase chain reaction etiology immuno-histochemistry

Real-Time Microwave Exposure Induces Calcium Efflux in Primary Cardiomyocytes

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Real-Time Microwave Exposure Induces Calcium Efflux in Prima...

中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次中青年学者科技论坛

作者： ZHANG Jing WANG Hui ZHANG Bo ZHOU Hongmei peng ruiyun Institute of Radiation Medicine Academy of Military Medical Sciences

Objective To detect the effects of microwave on calcium levels in primarycardiomyocytes by the real-time microwave exposure combined with laser scanning confocal microscopy. Methods The primary cardiomyocytes were cultured and labeled with probes,including Fluo-4 AM, Mag-Fluo-AM, and Rhod-2, to reflect the levels of whole calcium [Ca2+],endoplasmic reticulum calcium [Ca2+]ER, and mitochondrial calcium [Ca2+]MIT, respectively. Then,the cells were exposed to a pulsed microwave of 2.856 GHz with specific absorption rate (SAR)values of 0, 4, and 40 W/kg for 6 min to observe the changes in calcium *** The results showed that there were no significant changes in the levels of [Ca2+],[Ca2+]ER and[Ca2+]MIT in primary myocardial cells after 4W/kg microwave radiation. The 40 W/kg microwave radiation caused a significant decrease in the levels of [Ca2+],[Ca2+]ER, and [Ca2+]MIT in primary cardiomyocytes. The mitochondria were more sensitive to microwave exposure than the endoplasmic reticulum. Conclusion The calcium efflux was occurred during microwave exposure in primary cardiomyocytes. Additionally, mitochondria was the most sensitive organelle.

关键词： Real time Microwave Calcium Primary cardiomyocytes

Accumulative effects of microwave radiation on cardiac injury in rats

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Accumulative effects of microwave radiation on cardiac injur...

中国解剖学会2021年年会

作者： Jing Zhang Bo Zhang Binwei Yao Xinping Xu Hui Wang Li Zhao Ji Dong Haoyu Wang Shengzhi Tan ruiyun peng Department of Experimental Pathology Beijing Institute of Radiation Medicine Department of Pathology Beiya Orthopaedic Hospital

According to the current researches,we could say microwave radiation can cause heart *** plays an important role in the process of heart disease and injury, however,the effects induced by microwave radiation of different frequencies in a rat’s heart are still unclear,which are dose-dependent,frequency-dependent and accumulative.

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Long term effects of microwave radiation on the learning and memory and the expression of NMDA receptors

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Long term effects of microwave radiation on the learning and...

第九届全国生物医学体视学学术会议、第十二届全军军事病理学学术会议、第八届全军定量病理学学术会议

作者： Hui Wang ruiyun peng Li Zhao Shuiming Wang Yabing Gao Lifeng Wang Hongyan Zuo Ji Dong Xinping Xu Hongmei Zhou Zhentao Su Department of Experimental Pathology and Division of Radiation Protection and Health Physics BeijingInstitute of Radiation Medicine

Purpose:In the present study,we intended to investigate whether the high power microwave could cause the continuous disorders of learning and memory inWistarrats and to explore the underlying *** and methods:80Wistar rats were exposed to a microwave source at a power density of0mW/cmand50 mW/cm microwave for 6 *** spatial memory ability,the structure of the hippocampus,contents of amino acids neurotransmitters in hippocampus andthe expression of N-methyl-D-aspartic acid receptors（NMDAR） subunit 1,2A and 2B（NR1,NR2 A and NR2B）were detected at 1 m,3 m,6 m,9 m,12 m and 18 m after microwave ***:Our results showed that the microwave exposed rats showed consistent deficiencies in spatial learning and *** level of amino acid neurotransmitters also decreased after microwave *** ratio of glutamate（Glu） and gammaaminobutyric acid（GABA）significantly decreased at 6 ***,the hippocampus showed varying degrees of degeneration of neurons,increased postsynaptic density and blurred synaptic clefts in the exposure *** NRland NR2 B expression showed a significant decrease,especially the NR2 B ***:This studyindicated that the content of amino acids neurotransmitters,the expression of NMDAR subunits and the variation of hippocampal structure might contribute to the long term cognitive impairment after microwave exposure.

关键词： Microwave learning and memory NMDAR hippocampus rats

SMAD PATHWAY PLAY A ROLE OF IN THE ABNORMALITIES OF LUNG FIBROBLASTS INDUCED BY γ RAY IRRADIATION

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SMAD PATHWAY PLAY A ROLE OF IN THE ABNORMALITIES OF LUNG FIB...

第十三届国际体视学大会

作者： Yang Li,ruiyun peng,Liangwen Song,Leilei Yang,Shaoxia Wang,Dewen Wang Beijing Institute of Radiation Medicine No.27 Taiping Road,Beijing 100850,China

Objective:Radiation pulmonary fibrosis(RPF) is a sequel of both radiotherapy and accidental *** predominant characteristics are massive deposition of extracellular matrix and excessive fibroblast proliferation. Lung fibroblasts(LFs) play a central role in the pathogenesis of ***β1 is a master switch and therapeutic target of *** pathway is the key signal transducer downstream TGFβ*** the studies on the role of Smad pathway in the abnormalities of irradiated LFs might get a new insight into the pathogenesis of RPF. Methods:Rat lung fibroblasts(RLFs ) were isolated from rat lung by trypsin disaggregation to establish a stable model.γray,SB203580 or WP631 were used to treat *** changes of the Smad pathway and cellular biological behaviors of RLFs were determined by immunoblotting,immunohistochemistry,coimmunoprecipitation, EMSA,MTT and image anylasis. Results:γray irradiation could stimulate the proliferation,transformation,typeⅠcollagen and PAI-1 expressions of *** the same time,the formation and nucleus translocation of Smad3-4 complex were enhanced. Moreover,γray could facilitate the activation of transcription factors Smad3-4,SP1 and *** with SB203580 or WP631,irradiated RLFs expressed less typeⅠcollagen and P-Smad3,and also the activation of transcription factors Smad3-4,SP1 and AP1 in RLFs were inhibited. Conclusions:γray can facilitate the activation of Smad signal pathway in RLFs,and that the activation of this pathway play a role in the proliferation,transformation and typeⅠcollagen expressions of irradiated RLFs.

关键词： γray lung fibroblast Smad pathway

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中国生物医学体视学发展现状与展望

中国体视学与图像分析 2016年第1期21卷 66-75页

作者：李杨彭瑞云军事医学科学院放射与辐射医学研究所北京100850

本综述首先回顾和总结了我国近10年来生物医学体视学发展现状,包括生物医学体视学方法的建立和完善、体视学方法在生物医学和临床病理研究中的应用,如体视学在放射病理学、电磁辐射损伤研究、正常组织结构或各种疾病动物模型研究、肿瘤... 详细信息

本综述首先回顾和总结了我国近10年来生物医学体视学发展现状,包括生物医学体视学方法的建立和完善、体视学方法在生物医学和临床病理研究中的应用,如体视学在放射病理学、电磁辐射损伤研究、正常组织结构或各种疾病动物模型研究、肿瘤病理诊断中的应用,以及红外热像定量技术、皮肤镜图像分析技术和骨形态计量技术及其应用等;进而从体视框及其他体视学方法和程序在生物医学中的应用等方面,比较分析了国内外生物医学体视学的发展现况;最后提出了我国生物医学体视学的发展趋势及发展策略。

关键词：生物医学体视学现状与展望综述

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太赫兹波辐射生物效应研究现状与展望

中华放射医学与防护杂志 2018年第3期38卷 230-235页

作者：伊如汉彭瑞云王波赵黎内蒙古民族大学第二临床医学院通辽028000 内蒙古民族大学第二临床医学院(内蒙古林业总医院)病理科牙克石022150 军事科学院军事医学研究院辐射医学研究所北京100850

太赫兹波频率位于宏观电子学和微观光子学之间，因此，这种辐射既表现出类似光子特性，又表现出类似电子特性，具有很大的应用价值和潜力。随着全世界范围内的大力发展，太赫兹源与检测技术得到了突破性的进展，国际科技界认为，太赫兹... 详细信息

太赫兹波频率位于宏观电子学和微观光子学之间，因此，这种辐射既表现出类似光子特性，又表现出类似电子特性，具有很大的应用价值和潜力。随着全世界范围内的大力发展，太赫兹源与检测技术得到了突破性的进展，国际科技界认为，太赫兹波可能引发科学技术的革命性发展。特别是在生物医学领域。近年来，太赫兹波辐射对生物的影响研究已成为生命科学领域的重大新课题。目前，这一领域的研究国内外均属起步阶段。因此，本文就太赫兹波特性、生物医学应用、生物效应及其作用机制等进行综述，并对未来发展方向进行展望，旨在为太赫兹波生物医学研究提供参考。

关键词：太赫兹波生物效应机制