检索结果-南通市图书馆

Unlocking the future:Mitochondrial genes and neural networks in predicting ovarian cancer prognosis and immunotherapy response

引用

World Journal of Clinical Oncology 2025年第1期16卷 43-52页

作者： Zhi-Jian Tang Yuan-Ming Pan Wei Li rui-qiong ma Jian-Liu Wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Cancer Research Center Beijing Chest HospitalBeijing 101149China Department of Thoracic Surgery Sichuan Provincial People's HospitalChengdu 610072Sichuan ProvinceChina

BACKGROUND Mitochondrial genes are involved in tumor metabolism in ovarian cancer(OC)and affect immune cell infiltration and treatment *** To predict prognosis and immunotherapy response in patients diagnosed with OC using mitochondrial genes and neural *** Prognosis,immunotherapy efficacy,and next-generation sequencing data of patients with OC were downloaded from The Cancer Genome Atlas and Gene Expression *** genes were sourced from the MitoCarta3.0 *** discovery cohort for model construction was created from 70% of the patients,whereas the remaining 30% constituted the validation *** the expression of mitochondrial genes as the predictor variable and based on neural network algorithm,the overall survival time and immunotherapy efficacy(complete or partial response)of patients were *** In total,375 patients with OC were included to construct the prognostic model,and 26 patients were included to construct the immune efficacy *** average area under the receiver operating characteristic curve of the prognostic model was 0.7268[95% confidence interval(CI):0.7258-0.7278]in the discovery cohort and 0.6475(95%CI:0.6466-0.6484)in the validation *** average area under the receiver operating characteristic curve of the immunotherapy efficacy model was 0.9444(95%CI:0.8333-1.0000)in the discovery cohort and 0.9167(95%CI:0.6667-1.0000)in the validation *** The application of mitochondrial genes and neural networks has the potential to predict prognosis and immunotherapy response in patients with OC,providing valuable insights into personalized treatment strategies.

关键词： Ovarian cancer Mitochondria Prognosis Immunotherapy Neural network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-fidelity resonant tunneling passage in three-waveguide system

引用

Chinese Physics B 2022年第2期31卷 320-328页

作者： rui-qiong ma Jian Shi Lin Liu Meng Liang Zuo-Liang Duan Wei Gao Jun Dong School of Electronic Engineering Xi'an University of Posts ami TelecommunicationsXi'an 710121China

An N-stage three-waveguide system is proposed to improve the robustness and the fidelity of the resonant tunneling *** analytic solutions to the tunneling dynamics at the output are *** the number of subsystems increases,tunneling efficiency approaches to 100%in a large range and resonant tunneling is robust against variations in the phase mismatch and peak tunneling rate.

关键词： amplitude control resonant tunneling passage robustness

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

.ow Programmed Cell Death 5 Expression is a Prognostic Factor in Ovarian Cancer

引用

Chinese Medical Journal 2015年第8期128卷 1084-1090页

作者： Li Gao Xue Ye rui-qiong ma Hong-Yan Cheng Hong-Jing Han Heng Cui Li-Hui Wei Xiao-Hong Chang Gynecology Oncology Center Peking University People's Hospital Beijing 100044 China Center for Reproductive Medicine Peking University People's Hospital Beijing 100044 China

Background： Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 （PDCD5） in patients with ovarian cancer. Methods： Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines （SKOV3, CAOV3, ES2, OV1, 3AO, and HOC 1 A） and one normal ovarian epithelial cell line （T29） using reverse transcription polymerase chain reaction, Western blotting, and flow cytolnetry. Alter inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin （either alone or combined）, apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer. Results： PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells （15.96 ± 2.07%, vs. 3.17 ± 1.45% in controls）. In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival （P 〈 0.05, R = 0.7139 for survival）. Conclusions： PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.

关键词： Ovarian Cancer Poor Prognosis Programmed Cell Death 5

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of Circulating Endometrial Cells as a Biomarker for Endometriosis

引用

Chinese Medical Journal 2017年第19期130卷 2339-2345页

作者： Ying Chen Hong-Lan Zhu Zhe-Wen Tang Kuang Hong Neoh Dong-Fang Ouyang Heng Cui Hong-Yan Cheng rui-qiong ma Xue Ye Ray P. S. Han Xiao-Hong Chang Gynecological Oncology Center Peking University People's Hospital Beijing 100044 China Department of materials Science and Engineering Peking University Beijing 100871 China Department of Mechanical and Industrial Engineering University of Toronto Toronto Ontario M5S 3G8 Canada

Background： Circulating endometrial cells （CECs） have been reported to be present in the peripheral blood of women with endometriosis （EM）, providing clear and specific evidence of the presence of ectopic lesions. In this study, we established a method with a high detection rate of CECs, assessed the diagnostic value of CECs for EM and compared with serum CA125, and proposed a hypothesis for the pathogenesis of EM from the new perspective of CECs. Methods： The participants were enrolled prospectively from October 2015 to July 2016. The peripheral blood samples were collected from 59 participants, and the blood cells were isolated for immunofluorescence staining via microfluidic chips. The cells that were positive for vimentin/cytokeratin and estrogen/progesterone receptor and negative for CD45 were identified as CECs. The serum CA125 level was tested with electrochemiluminescence immunoassay. Results： The detection rate of CECs reached 89.5% （17/19） in the EM group, which was significantly higher than that of the control group （15.0% [6/40], P 〈 0.001） and was independent of menstrual cycle phases. Furthermore, a positive CEC assay detected 4/5 cases of Stage Ⅰ–Ⅱ EM. In contrast, a positive CA125 test had limited value in detecting EM （13/19, 68.4%） and detected only one case of Stage Ⅰ–Ⅱ EM. Conclusion： CECs are promising biomarkers for EM with great potential for a noninvasive diagnostic assay.

关键词： Biomarker Circulating Endometrial Cells Clinical Diagnosis Endometriosis Pathogenesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

玻璃化冷冻保存羊卵巢组织异种移植的实验研究

引用

生殖与避孕 2011年第5期31卷 329-334页

作者：程洪艳叶雪昌晓红成夜霞马瑞琼崔恒北京大学人民医院妇科肿瘤中心北京100044

目的:探讨羊卵巢组织玻璃化冷冻复苏后异种移植的卵泡生长发育情况。方法:取绵羊的卵巢,将卵巢皮质切块,应用玻璃化快速冷冻法保存。羊卵巢组织片复苏后在裸鼠的颈部皮下移植,每只裸鼠移植2块组织,于移植1个月后获取移植物,HE染色观察... 详细信息

目的:探讨羊卵巢组织玻璃化冷冻复苏后异种移植的卵泡生长发育情况。方法:取绵羊的卵巢,将卵巢皮质切块,应用玻璃化快速冷冻法保存。羊卵巢组织片复苏后在裸鼠的颈部皮下移植,每只裸鼠移植2块组织,于移植1个月后获取移植物,HE染色观察存活卵泡的情况。结果:共移植组织16块,取材时肉眼可见其中一块组织纤维化萎缩,镜下见此块组织纤维化,移植组织存活率87.5%(14/16)。HE染色观察每只移植小鼠的2块移植物中基本只有1块有可视卵泡。1只鼠未见可视卵泡;1只鼠见1个不典型原始卵泡;1只鼠有2个不典型原始卵泡;余下5只鼠的移植卵巢组织中均可见多个典型的存活卵泡。结论:羊卵巢组织经冷冻复苏后能够成功移植于裸鼠的颈部皮下,大部分卵泡处于良好的存活状态。

关键词：羊卵巢组织冷冻保存异种移植

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

引用

Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu rui Wang Hanyue Zhang Xiou Wang Sijia Zhou Bo ma Yan Luan Xin Wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

缺血性脑卒中铁死亡特征基因NFE2L2的鉴定与验证

引用

中国组织工程研究 2025年第7期29卷 1466-1474页

作者：王咪马书杰刘杨齐瑞上海中医药大学附属岳阳中西医结合医院康复医学科上海市200437 上海中医药大学上海市201203 上海市第二康复医院上海市200431

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血... 详细信息

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血性脑卒中的作用。方法:基于GEO数据库和FerrDb数据库选取符合条件的缺血性脑卒中相关数据集和铁死亡表达数据集,通过t检验筛选铁死亡相关差异基因。对铁死亡相关差异基因进行GO功能富集分析与KEGG信号通路富集分析。通过PPI网络分析和机器学习筛选缺血性脑卒中铁死亡的特征基因,利用ROC分析和GSEA分析探究特征基因的准确性和生物功能。然后进行细胞实验,将HT22细胞分为对照组与缺血性脑卒中组,对照组不作任何干预,缺血性脑卒中组加入0.1 mol/L的H_(2)O_(2)干预24 h诱导细胞氧化应激和铁死亡,通过实时荧光定量RT-PCR和Western Blot验证铁死亡的发生和特征基因表达。结果与结论:(1)共获取45个铁死亡相关差异基因,GO和KEGG富集分析发现差异基因与氧化应激、自噬、铁死亡、脂肪细胞因子信号通路和线粒体代谢密切相关。(2)通过PPI网络中的MCODE插件和cytoHubba插件与机器学习中的LASSO算法和SVM-RFE算法共鉴定出1个铁死亡特征基因核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,NFE2L2)。(3)对NFE2L2进行ROC曲线分析,发现在训练集和验证集中构建的诊断预测模型具有良好的准确性与特异性;对NFE2L2进行GSEA分析,发现特征基因通过免疫、炎症反应、氨基酸代谢及神经因子调控等方面参与缺血性脑卒中发病机制的调控。(4)细胞实验的RT-PCR和Western Blot分析表明,与对照组对比,缺血性脑卒中组中的酰基辅酶A合成酶长链家族成员4 mRNA和蛋白表达水平显著增高(P<0.05),谷胱甘肽过氧化物酶4 mRNA和蛋白表达水平显著降低(P<0.05);与对照组对比,缺血性脑卒中组中特征基因NFE2L2 mRNA和蛋白表达水平显著增高(P<0.05)。(5)上述结果证实,缺血性脑卒中与铁死亡密切相关,靶向特征基因NFE2L2可以为研究和治疗缺血性脑卒中提供一定的思路与方向。

关键词：缺血性脑卒中铁死亡生物信息学 HT22细胞机器学习特征基因细胞实验 NFE2L2 ASCL4 GPX4

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic Modeling for Gas-Electricity Combined Systems with Wind Power and its Stability Control

引用

CSEE Journal of Power and Energy Systems 2023年第6期9卷 2386-2393页

作者： rui ma Pingping He Changsha University of Science&Technology Changsha 410114China

The micro turbine(MT)is scheduled to address wind power uncertainties and thus lead to greater variations in the state of natural gas system(NGS).To meet this challenge,first,a dynamic model of a gas-electricity combined system with wind power is established,the dynamic model of the NGS is based on differential equations,so that it can easily connect with the MT model;and a droop control module is added to the Rowen's MT model,so that the improved model can adjust the output power according to the scheduling ***,a stability control method based on the variable regulation ratio gas pressure regulator is proposed,the essence of this method is to reduce the variation of natural gas storage in the gas pipeline,so as to ensure the stability of *** simulation results show that the dynamic model is correct and the stability control method is effective.

关键词： Dynamic behaviors integrated energy system stability control method wind power

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cooperative effects of Mo,V and Zr additions on the microstructure and properties of multi-elemental Nb-Si based alloys

引用

Journal of materials Science & Technology 2023年第1期132卷 27-41页

作者： rui ma Xiping Guo State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China

Eight multi-elemental Nb-Si-based alloys with various Mo,V and Zr contents were prepared by vacuum non-consumable arc *** cooperative alloying effects of Mo,V and Zr on the arc-melted and heat-treated microstructure,mechanical properties as well as oxidation resistance at 1250°C of the alloys were evaluated *** results show that except for adding Mo solely,additions of Mo,V and Zr change the microstructure from eutectic to *** additions of Mo,V and Zr suppress the formation ofα(Nb,X)5 Si 3(“X”represents the alloying elements that substitute for Nb in the lattices),whilst promoting the formation ofγ(Nb,X)5 Si *** heat treatment at 1450°C for 50 h promotes the formation of(Nb,X)3 Si phase in the Zr-containing *** with either Mo or Zr improves,and their composite additions more obviously improve the compressive yield strength at 1250°C as well as the microhardness ofγ(Nb,X)5 Si *** room temperature fracture toughness of the alloys is enhanced by sole and composite additions of V and Zr,while it is deteriorated by the addition of *** sole addition of Mo,V or Zr improves the oxidation resistance at 1250°C,the composite additions of V with Mo/Zr(especially V-Mo-Zr)degrade the oxidation resistance at 1250°C.

关键词： Multi-elemental Nb-Si based ultrahigh temperature alloy Alloying effect Microhardness Room temperature fracture toughness High-temperature compressive yield strength Oxidation resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment

引用

Chinese Chemical Letters 2024年第7期35卷 222-226页

作者： rui Wang He Qi Haijiao Zheng qiong Jia College of Chemistry Jilin UniversityChangchun 130012China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun 130012China

Metal-organic frameworks(MOFs)combined with specific ligands are highly adaptable smart materials that can respond to external and physiological *** this study,we introduced a pyridinyl zwitterionic ligand with light/pH dual response into magnetic MOF composite(Fe_(3)O_(4)@ZW-MOF)for enrichment of phosphorylated peptides for the first *** introduction of the developed ligand gives MOF material dual response *** stimulation affects the generation/disappearance of free radicals of the pyridine derivative,resulting in a change in the charge gradient of the zwitterion,and zwitterion can also regulate the p H of the solution by adding acid or ***,the reversible capture and release of phosphorylated peptides can be easily achieved by adjusting light and *** established phosphorylated peptide enrichment platform exhibits high sensitivity(detection limit of 1 fmol),high selectivity(β-casein:BSA,1:1000),and good reusability(7 cycles).In addition,the method was applied to the enrichment of phosphorylated peptides in complex systems(non-fat milk and human serum),demonstrating the feasibility of this method for phosphoproteom *** conclusion,the synthesized Fe_(3)O_(4)@ZW-MOF is a promising MOF material,which provides the possibility to advance the application of responsive MOFs materials in proteomics.

关键词： Light pH Dual-response Metal-organic frameworks Phosphorylated peptide

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：