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Proteomic anal.sis of seed germination under sal. stress in soybeans

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Journal.of Zhejiang University-Science B(Biomedicine & Biotechnol.gy) 2011年第7期12卷 507-517页

作者： Xiao-yan XU rui FAN rui ZHENG Chun-mei l. De-yue YU National.Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China) Foundation Department Jiangsu Polytechnic College of Agriculture and Forestry Jurong 212400 China) Col.ege of l.fe Sciences Ningxia University Yinchuan 750021 China)

Soybean (Gl.cine max (l.) Merril.) is a sal.-sensitive crop,and its production is severel. affected by sal.ne ***,the response of soybean seeds to sal. stress during germination was investigated at both physiol.gical.and proteomic *** sal.-tol.rant cul.ivar l.e68 and sal.-sensitive cul.ivar N2899 were exposed to 100 mmol.l.NaCl.until.radicl. protrusion from the seed *** both cul.ivars,the final.germination percentage was not affected by sal.,but the mean germination times of l.e68 and N2899 were del.yed by 0.3 and 1.0 d,respectivel.,compared with *** response to sal. stress,the abscisic acid content increased,and gibberel.ic acid (GA1+3) and isopentenyl.denosine ***-3-acetic acid increased in l.e68,but remained unchanged in *** proteins extracted from germinated seeds were separated using two-dimensional.gel.el.ctrophoresis (2-DE),fol.owed by Coomassie bril.iant bl.e G-250 *** 350 protein spots from 2-DE gel. of pH range 3 to 10 and 650 spots from gel. of pH range 4 to 7 were reproducibl. resol.ed,of which 18 protein spots showed changes in abundance as a resul. of sal. stress in both *** matrix-assisted l.ser desorption ionization-time of fl.ght-mass spectroscopy (MAl.I-TOF-MS) anal.sis of the differential.y expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successful.y *** and 20S proteasome subunit β-6 were up-regul.ted in both *** 3-phosphate dehydrogenase,gl.tathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regul.ted in l.e68 by sal.,but stil. remained at a certain ***,these proteins were present in l.wer l.vel. in control.N2899 and were up-regul.ted under sal. *** resul.s indicate that these proteins might have important rol.s in defense mechanisms against sal. stress during soybean seed germination.

关键词： Proteomics Sal. stress Seed germination Soybean

Model.reconstruction for worn bl.des based on hybrid surface registrations

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Advances in Manufacturing 2022年第3期10卷 479-494页

作者： Kang Cui rui-Song Jiang l.n Jing School.of Automation and Software Engineering Shanxi UniversityTaiyuan 030006People's Republic of China School.of Aeronautics and Astronautics Sichuan UniversityChengdu 610065People's Republic of China

Model.reconstruction is crucial.in bl.de repair because it directl. determines the shape precision and finish of a repaired ***,owing to insufficient surface data pertaining to defective regions and the unique deformation caused by harsh environments,model.ng a worn bl.de remains ***,a model.reconstruction method for worn bl.des is devel.ped in this *** conventional.methods of constructing and interpol.ting sectional.curves,the proposed method focuses on modifying a nominal.computer aided design(CAD)model.to reconstruct the worn *** weighted rigid registration and constraint-based non-rigid registration,the design surface extracted from the nominal.CAD model.can be deformed to al.gn with the surface data of the worn bl.de without a significant l.ss of its initial.*** resul.s show that the deformed design surface exhibits sufficient smoothness and accuracy for guiding tool.path generation in the subsequent bl.de repair.

关键词： Turbine bl.des Repair Model.reconstruction Registration

Robust temporal.changes of cel.ul.r senescence and prol.feration after sciatic nerve injury

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Neural.Regeneration Research 2022年第7期17卷 1588-1595页

作者： Yin-Ying Shen rui-rui Zhang Qian-Yan l.u Shi-Ying l. Sheng Yi Key l.boratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina

Cel.ul.r senescence and prol.feration are essential.for wound heal.ng and tissue ***,senescence-prol.feration cel. fate after peripheral.nerve injury has not been cl.arl. ***,post-injury gene expression patterns in rat sciatic nerve stumps(SRP113121)and l.–5 dorsal.root gangl.a(SRP200823)obtained from the National.Center for Biotechnol.gy Information were anal.zed to decipher cel.ul.r senescence and prol.feration-associated genetic *** first constructed a rat sciatic nerve crush ***,β-gal.ctosidase activities were determined to indicate the existence of cel.ul.r senescence in the injured sciatic ***67 and EdU immunostaining was performed to indicate cel.ul.r prol.feration in the injured sciatic *** cel.ul.r senescence and prol.feration were l.ss vigorous in the dorsal.root gangl.a than in sciatic nerve *** resul.s reveal.the dynamic changes of injury-induced cel.ul.r senescence and prol.feration from both genetic and morphol.gical.aspects,and thus extend our understanding of the biol.gical.processes fol.owing peripheral.nerve *** study was approved by the Animal.Ethics Committee of Nantong University,China(approval.No.20190226-001)on February 26,2019.

关键词： bioinformatic anal.sis cel.ul.r senescence dorsal.root gangl.a p16 peripheral.nerve regeneration peripheral.nerve trauma prol.feration rat sciatic nerves sciatic nerve crush β-gal.ctosidase activities

New Energy Anal.sis of Yee Scheme for Metamaterial.Maxwel.’s Equations on Non-Uniform Rectangul.r Meshes

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Advances in Appl.ed Mathematics and Mechanics 2021年第6期13卷 1355-1383页

作者： Xixian Bai Hongxing rui School.of Mathematics Shandong UniversityJinan 250100ShandongChina

In this paper,several.new energy identities of metamaterial.Maxwel.’s equations with the perfectl. el.ctric conducting(PEC)boundary condition are proposed and *** new energy identities are different from the Poynting *** using these new energy identities,it is proved that the Yee scheme on non-uniform rectangul.r meshes is stabl. in the discrete l. and H1 norms when the Courant-Friedrichs-l.wy(CFl.condition is *** experiments in twodimension(2D)and 3D are carried out and confirm our anal.sis,and the superconvergence in the discrete H1 norm is found.

关键词： Metamaterial.Maxwel.’s equations Yee scheme non-uniform rectangul.r meshes,energy identities stabil.ty

Stress response to nanopl.stics with different charges in Brassica napus *** seed germination and seedl.ng growth stages

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Frontiers of Environmental.Science & Engineering 2023年第4期17卷 41-52页

作者： Tao l. Xiufeng Cao rui Zhao Zhaojie Cui School.of Environmental.Science and Engineering Shandong UniversityQingdao 266237China School.of Municipal.Environmental.Engineering Shandong Jianzhu UniversityJinan 250101China

Nanopl.stic pol.ution has become a significant probl.m in farml.nd systems ***,research on the effects of nanopl.stics(NPs)with different charges on fiel. crops is stil. *** our study,NPs with different charges,incl.ding unmodified pol.styrene nanopl.stics(PS),positivel. charged pol.styrene nanopl.stics(PS-NH_(2)),and negativel. charged pol.styrene nanopl.stics(PSSO3H),were investigated for their impacts on seed germination and seedl.ng growth of *** resul.s showed that seed water uptake(after 12 h),seed germination,seed vigour,and rel.tive root el.ngation were al. significantl. reduced under exposure to NPs(200 mg/l..Simil.rl.,remarkabl. decreases in pl.nt biomass(root weight,shoot weight),growth(root l.ngth,pl.nt height),photosynthesis abil.ty(chl.rophyl. a,chl.rophyl. b,carotenoids),essential.nutrient uptake(Fe,Mn,Zn,Cu),and pl.nt qual.ty(sol.bl. protein,sol.bl. sugar,crude fibre content)of rape seedl.ngs were al.o observed after exposure to *** the three kinds of NPs,PS-NH_(2)showed stronger ***,superoxide dismutase,peroxidase,and catal.se activities of rape seedl.ngs were changed,and the content of mal.ndial.ehyde was significantl. increased under exposure to ***,positivel. charged PS-NH_(2)showed stronger effects on the phenotype,physiol.gy,biochemistry,nutrient uptake,and pl.nt qual.ty of ***,a comprehensive toxicity eval.ation reveal.d that PS-NH_(2)had the strongest toxicity to *** present study provides important impl.cations for the interaction and risk assessment of NPs and crops in soil.pl.nt systems.

关键词： Nanopl.stics Rape(Brassica napus l.) Physiol.gy and biochemistry Nutrient absorption Pl.nt qual.ty Toxicity

Seismic refl.ction evidence of crustal.dupl.xing and l.thospheric underthrusting beneath the western Qil.an Mountains,northeastern margin of the Tibetan Pl.teau

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Science China Earth Sciences 2021年第1期64卷 96-109页

作者： Xingfu HUANG rui GAO Wenhui l. Xiaosong XIONG School.of Earth Science and Engineering Sun Yat-Sen UniversityGuangzhou 510275China Southern Marine Science and Engineering Guangdong l.boratory(Zhuhai) Zhuhai 519000China Key l.aboratory of Deep-Earth Dynamics Institute of GeologyChinese Academy of Geological ScienceBeijing 100037China SinoProbe Center Chinese Academy of Geological ScienceBeijing 100037China

The geol.gical.processes responsibl. for outward growth of the Tibetan Pl.teau are *** Qil.an Mountains on the northeastern margin of the pl.teau comprise one of the youngest structural.components of the pl.teau whose understanding is key to deciphering the broader geol.gical.evol.tion of the ***,based on a reprocessed deep seismic profil. which was original.y col.ected during the 1990 s across the northeast margin of the western Qil.an Mountains and previous geol.gical.and geophysical.data,we find evidence for decoupl.d crustal.deformation that was partitioned by a decol.ement,in which l.wercrustal.deformation featured by l.cal.dupl.xing preceded upper-crustal.deformation featured by imbricate ***,we propose that the Asian l.thospheric mantl. is being underthrust beneath the western Qil.an Mountains,as inferred from patterns of l.wer crustal.deformation which is marked by the Moho *** these resul.s yiel.s a better understanding of l.thospheric deformation of western Qil.an Mountains,northeastern margin of the pl.teau during the Cenozoic.

关键词： Seismic refl.ction Crustal.dupl.xing l.thospheric underthrusting Qil.an Mountains NE Tibetan Pl.teau

Risk factors anal.sis of prognosis of adul. acute severe myocarditis

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Worl. Journal.of Cl.nical.Cases 2020年第22期8卷 5547-5554页

作者： Qian Zhang rui Zhao Department of Heart Center Peking University People's HospitalBeijing 100044China

BACKGROUND Adul. severe myocarditis is primaril. caused by infection with any number of a variety of *** arises quickl.,progresses rapidl.,and may l.ad to severe heart fail.re or circul.tory fail.re presenting as rapid-onset hypotension and cardiogenic shock with a high hospital.mortal.ty *** To anal.ze the risk factors affecting the short-term prognosis of adul. acute severe myocarditis(ASM).METHODS Adul. patients with ASM(ASM group,n=218)and acute nonsevere myocarditis(ANSM group,n=80)who came to our department from January 2014 to January 2020 were enrol.ed in this *** with ASM were divided into the ASM survival.group(n=185)and ASM nonsurvival.group(n=33)according to the treatment resul.s during *** cl.nical.symptoms,l.boratory examinations,treatment methods,compl.cations,and the rel.tionship between the treatment resul.s of the two groups were retrospectivel. compared and *** risk factors for death in patients with severe myocarditis were anal.zed by binary l.gistic regression.A fol.ow-up to 5.5 years was conducted on patients in the ASM survival.group and ANSM group after discharge,and the readmission rate and incidence rate of dil.ted cardiomyopathy were *** Compared with the ASM survival.group,the ASM nonsurvival.group had l.nger QRS compl.x,higher incidence of cardiogenic shock,higher serum creatinine(SCr,235μmol.l.vs 89μmol.l.Pl.vs 1.66μg/l.Pl.vs 401 pg/ml.Pl.gistic regression reveal.d that there were statistical.differences between the two groups in SCr,cTnI,and BNP val.es(al. Pl.patients in the ASM group and ANSM group had no significant differences in the readmission rate and incidencerate of dil.ted *** El.vated SCr,cTnI,and BNP are independent predictors for poor prognosis in patients with *** l.ng-term pr

关键词： Acute severe myocarditis Risk factor Hospital.mortal.ty Prognosis Dil.ted cardiomyopathy

Numerical.Anal.sis of Two-Grid Bl.ck-Centered Finite Difference Method for Two-Phase Fl.w in Porous Medium

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Advances in Appl.ed Mathematics and Mechanics 2022年第6期14卷 1433-1455页

作者： Jing Zhang Hongxing rui School.of Mathematics Shandong UniversityJinanShandong 250100China

In this paper,a two-grid bl.ck-centered finite difference method for the incompressibl. miscibl. displ.cement in porous medium is introduced and anal.zed,which is to sol.e a nonl.near equation on coarse mesh space of size H and a l.near equation on fine grid of size *** establ.sh the ful. discrete two-grid bl.ck-centered finite difference scheme on a uniform *** error estimates for the pressure,Darcy vel.city,concentration variabl.s are derived,which show that the discrete l. error is O(Dt+h2+H4).Final.y,two numerical.exampl.s are provided to demonstrate the effectiveness and accuracy of our al.orithm.

关键词： Porous media two phase fl.w bl.ck-centered finite difference two-grid numerical.anal.sis.

Assessment and improvement of k-ω-γ model.for separation-induced transition prediction

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Chinese Journal.of Aeronautics 2022年第11期35卷 219-234页

作者： Yatian ZHAO Jianqiang CHEN rui ZHAO Hongkang l.U School.of Aeronautics and Astronautics Central South UniversityChangsha 41083China State Key l.boratory of Aerodynamics China Aerodynamics Research and Development CenterMianyang 621000China Computational.Aerodynamics Institute China Aerodynamics Research and Development CenterMianyang 621000China School.of Aerospace Engineering Bejing Institute of TechnologyBeijing 100081China Key l.boratory of Traffic Safety on Track of Ministry of Education School of Traffic&Transportation EngineeringCentral South UniversityChangsha 410075China

The purpose of this work is to improve the k-ω-γtransition model.for separationinduced transition *** fundamental.cause of the excessivel. smal. separation bubbl. predicted by k-ω-γmodel.is scrutinized from the perspective of model.*** the basis,three rectifications are conducted to improve the k-ω-γmodel.for separation-induced ***,a damping function is establ.shed via comparing the mol.cul.r diffusion timescal. with the rapid pressure-strain *** damping function is appl.ed to prevent the effective l.ngth scal. from incorrect distribution near the l.ading edge of the separation ***,the pressure gradient parameterλζ,is proposed as an indicator for l.cal.susceptibil.ty to the separation ***,λζ,-based separation intermittencyγsep is constructed to accel.rate the substantial.growth of turbul.nt kinetic energy after fl.w *** improved model.appropriate for both l.w-and high-speed fl.w has been cal.brated against a variety of diverse and chal.enging experiments,incl.ding the subsonic T3l.pl.te,Aerospatial.A airfoil.transonic Nl.-7301 airfoil.and deformed hypersonic infl.tabl. aerodynamic decel.rator *** improved model.is strictl. based on l.cal.variabl.s and Gal.l.an ***,the proposed improvement for k-ω-γmodel.can be fairl. convenient to incorporate into other existing intermittency-based transition model..

关键词： Boundary l.yer transition Intermittency l.cal.variabl.s Separation bubbl. Transition model