Proteomic analysis of seed germination under salt stress in soybeans
Proteomic analysis of seed germination under salt stress in soybeans作者机构：National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China) Foundation Department Jiangsu Polytechnic College of Agriculture and Forestry Jurong 212400 China) College of Life Sciences Ningxia University Yinchuan 750021 China)
出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)
年 卷 期：2011年第12卷第7期
页 面：507-517页
核心收录：
基 金：Project supported by the National Natural Science Foundation of China (No. 30800692) the National Basic Research Program (973) of China (Nos. 2010CB125906 and 2009CB118400) the National High-Tech R & D Program (863) of China (No. 2006AA10Z1C1)
主 题：Proteomics Salt stress Seed germination Soybean
摘 要：Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop,and its production is severely affected by saline ***,the response of soybean seeds to salt stress during germination was investigated at both physiological and proteomic *** salt-tolerant cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed *** both cultivars,the final germination percentage was not affected by salt,but the mean germination times of Lee68 and N2899 were delayed by 0.3 and 1.0 d,respectively,compared with *** response to salt stress,the abscisic acid content increased,and gibberellic acid (GA1+3) and isopentenyladenosine ***-3-acetic acid increased in Lee68,but remained unchanged in *** proteins extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE),followed by Coomassie brilliant blue G-250 *** 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7 were reproducibly resolved,of which 18 protein spots showed changes in abundance as a result of salt stress in both *** matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially expressed proteins,the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were successfully *** and 20S proteasome subunit β-6 were up-regulated in both *** 3-phosphate dehydrogenase,glutathione S-transferase (GST) 9,GST 10,and seed maturation protein PM36 were down-regulated in Lee68 by salt,but still remained at a certain ***,these proteins were present in lower levels in control N2899 and were up-regulated under salt *** results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean seed germination.
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