检索结果-南通市图书馆

Effects of Arterially Infused Hydroalcoholic <i>Agaricus blazei</i>Extracts on Perfusion Pressure and Oxygen Uptake in the Bivascularly Perfused Rat Liver

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2015年第10期3卷 74-79页

作者： Andrea Luiza de Oliveira Valoto Jorgete Constantin Fabrício Bracht rosane marina peralta Adelar Bracht Faculdade Integrado de Campo Mourao Campo Mourao Brazil Department of Biochemistry Universidade Estadual de Maringá (UEM) Maringá Brazil Universidade Técnica Federal do Paraná (UTFPR) Campo Mourao Brazil

In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vein in monovascular perfusion (entry: portal vein;exit: hepatic vein). In the present communication we are presenting and discussing experiments done with the bivascularly perfused rat liver (entry: portal vein + hepatic artery;exit: hepatic vein) in order to verify if the hemodynamic effects also occur in the arterial bed. It was found that the A. blazei extract is also active when infused into the hepatic arterial bed, with differences in both sensitivity and nature of the effects on either perfusion pressure or oxygen consumption. Constriction of the arterial bed required much higher concentrations of the extract than the portal bed. The kinetics of the response was also different, with a biphasic instead of a monophasic response. These results provide a promising starting point for future studies aiming to bring to light more mechanistic details about these and possibly other effects.

关键词： Agaricus blazei Purinergic Action Hemodynamics Oxygen Uptake

Production of Enzymes and Biotransformation of Orange Waste by Oyster Mushroom, <i>Pleurotus pulmonarius</i>(Fr.) Quél.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2015年第1期5卷 1-8页

作者： Fabíola Dorneles Inácio Roselene Oliveira Ferreira Caroline Aparecida Vaz de Araujo rosane marina peralta Cristina Giatti Marques de Souza Federal Institute of Paraná-Campus Jacarezinho Jacarezinho Brazil Post-Graduation Program of Biological Sciences State University of Maringá Maringá Brazil Departament of Biochemistry State University of Maringá Maringá Brazil

The wood-decay fungi are able to bioconvert a wide variety of lignocellulosic residues due to the secretion of extracellular enzymes. The use of agricultural wastes as substrate for mushroom cultivation or enzymes production can help to solve environmental problems caused by inadequate discharge in the nature. The production of hydrolytic and oxidative enzymes by Pleurotus pulmonarius developed in solid state system using orange waste as substrate was evaluated in this work. Among the hydrolytic enzymes, pectinase was the main enzyme produced by the fungus, presenting the highest enzymatic activity of 9.4 U/mL after 35 days of cultivation. Considering the oxidative enzymes, laccase was the main enzyme produced with maximal activity of 12.2 U/mL obtained after 20 days of cultivation. Low enzyme levels of manganese peroxidase, β-glucosidase and β-xy-losidase were detected with activity peaks at the end of the cultivation. The enzymatic levels of amylase, carboxymethyl cellulase and xylanase were similar and less than 1.5 U/mL. No aryl-alcohol oxidase activity was detected. NDF, ADF and cellulose values increased during 45 days of cultivation. There was no lignin degradation during the study period and the fungus culture in orange solid waste caused protein enrichment in the substrate. Our results demonstrate that P. pulmonarius was an efficient producer of two important industrial enzymes, pectinase and laccase in a cheap solid state system using orange waste as substrate.

关键词： Orange Waste Biotransformation Pleurotus pulmonarius Basidiomycetes

The Action of p-Synephrine on Lipid Metabolism in the Perfused Rat Liver

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2017年第5期5卷 8-21页

作者： Juliany Fontoura da Silva-Pereira Andrea Luiza de Oliveira Valoto Livia Bracht Geferson de Almeida Goncalves rosane marina peralta Adelar Bracht Department of Biochemistry University of MaringaMaringaBrazil Faculdade Integrado Campo MouraoBrazil

p-synephrine and p-octopamine were found to increase lipolysis in adipocytes. The present study approaches the question if these compounds, natural products of the bitter orange (Citrus aurantium fruit), increase lipolysis and fatty acid oxidation in the liver. Experiments were done in the perfused rat liver. Non-recirculating hemoglobin-free perfusion was done using the Krebs/ Henseleit-bicarbonate buffer (pH 7.4) as perfusion fluid. Both p-synephrine and p-octopamine, at the concentrations of 100 μM, were found to stimulate the hepatic triacylglycerol lipase by 40% and 51%, respectively. These seem to be the maximal stimulations possible in the liver. In the perfused liver, p-synephrine, when present at an initial concentration of 500 μM, was able to increase the non-esterified fatty acid release after one hour of recirculating perfusion. The effects of p-synephrine on the oxidation of exogenously supplied [1-14C]octanoate and [1-14C]oleate were minimal. Only oxygen uptake, already stimulated by octanoate or oleate, was additionally increased by the infusion of p-synephrine. These results contrast with those obtained in a previous study with p-octopamine, which increased the production of 14CO2 from both [1-14C]octanoate and [1-14C]oleate. Apparently only the oxidation of endogenous fatty acids is stimulated by p-synephrine. On the other hand, both p-synephrine and p-octopamine stimulate the hepatic triacylglycerol lipase to a much lesser extent than the adipocyte lipase. It can be concluded that p-synephrine affects much more carbohydrate metabolism in the liver than lipid metabolism.

关键词： Hepatic Lipid Metabolism Lipolysis Fatty Acid Release Fatty Acid Oxidation

The in Vitro Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of Smallanthus sonchifolius (Yacon) Do Not Correlate with Their in Vivo Antioxidant Action in Diabetic Rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2016年第2期4卷 15-27页

作者： Ana Carla Broetto Biazon Mariana Marques Nogueira Wendt Júlia Rosa Moreira Cristiane Vizioli Castro Ghizoni Andreia Assunção Soares Sandra da Silva Silveira Anacharis Babeto de Sá-Nakanishi Ciomar Aparecida Bersani Amado rosane marina peralta Adelar Bracht Jurandir Fernando Comar Pharmacy Department Faculdades Integrado Campo Mourao Brazil Biochemistry Department University of Maringá Maringá Brazil Pharmacology and Therapeutics Department University of Maringá Maringá Brazil

Leaf and root extracts of Smallanthus sonchifolius (yacon), have antihyper-glycemic activity and antioxidant properties. The present study aims to compare the in vivo hepatic antioxidant activity of hydroalcoholic extracts of yacon leaves and roots in rats with streptozotocin-induced diabetes in terms of their in vitro antioxidant capacity. Rats were treated during 14 days with 1060 mg·Kg-1 root extract or 400 mg·Kg-1 leaf extract. The latter was richer in phenolics and possessed a much higher in vitro antioxidant activity. Both extracts prevented hyperglycemia in diabetic rats. The liver of diabetic rats presented increased levels of protein carbonyls and ROS and decreased activities of antioxidant enzymes. Treatment with both root and leaf extracts restored the protein carbonyl levels to normality. The root extract also restored the ROS levels to normality, but the leaf extract was not effective. The root extract was also more effective in restoring the activity of at least two important antioxidant enzymes (glucose 6-phosphate dehydrogenase and glutathione peroxidase). In terms of the antioxidant load (which was 17 times lower in the root extract treatment), the in vivo action of the root extract was more effective than the leaf extract in reducing the hepatic oxidative stress that accompanies diabetes.

关键词： Smallanthus sonchifolius Yacon Streptozotocin-Induced Diabetes Liver Oxidative Status