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BIOCELL 2022年第7期46卷 1751-1762页

作者： JIANMIN PAN xin li JUANJUAN li HUI liU ZHUONI HOU ZONGQI YANG liNG XU Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou310018China UWA School of Agriculture and Environment and The UWA Institute of Agriculture Faculty of ScienceThe University of Western AustraliaPerth6009Australia

Plant U-box(PUB)E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses,but little is known about U-box genes in Danshen(root of Salvia miltiorrhiza Bunge).Here,we identified and characterized 70 SmPUB genes based on its genome *** analysis of U-box genes from *** and Arabidopsis suggested that they can be clustered into seven subgroups(I–VII).Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved ***,qRT-PCR was applied to analyze the relative expression levels of U-box genes in *** roots and leaves under PEG-induced water deficit and salt *** revealed that the SmPUB genes exhibited stronger response to drought than to salt *** the best of our knowledge,this report is the first to perform genome-wide identification and analysis of the U-box gene family in ***,and the results provide valuable information for better understanding of the function of U-box in ***.

关键词： Salvia miltiorrhiza U-box E3 ubiquitin ligase Gene-family Abiotic stresses Expression pattern

Hyperosmolarity disrupts tight junction via TNF-α/MMP pathway in primary human corneal epithelial cells

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International Journal of Ophthalmology(English edition) 2022年第5期15卷 683-689页

作者： Yun Zhang Ming Yang Shi-xin Zhao li Nie li-Jun Shen Wei Han Eye Centre Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

AIM:To investigate the mechanism of the tight junction(TJ) disruption and the association between tumor necrosis factor(TNF)-α and matrix metalloproteinase(MMPs) under hyperosmotic condition in primary human corneal epithelial cells(HCECs).METHODS:The cultured HCECs were exposed to media which adding sodium chloride(Na Cl) for hyperosmolar stress or adding rh-TNF-α(10 ng/m L). NF-κB inhibitor(5 μmol/L) or GM-6001(potent and broad spectrum MMP inhibitor, 20 μmol/L)was added 1 h before that treatment. The integrity of TJ proteins was determined by immunofluorescent(IF) staining. The m RNA levels of TNF-α and MMPs were evaluated by quantitative reverse transcription polymerase chain reaction(RT-q PCR) and the protein expression by enzyme-linked immunosorbent assay(EliSA).RESULTS:TJ proteins ZO-1 and Occludin were disrupted in primary HCECs exposed to hyperosmotic medium. The m RNA expression and protein production of TNF-α increased significantly in hyperosmotic media at 500 m Os M. TNF-α mediated the expression and production of MMP-1, MMP-13, MMP-9, and MMP-3 stimulated by hyperosmotic stress. The production of MMPs in hyperosmolar media were increased through the increase of TNF-α. GM-6001 prevent the destruction of ZO-1 and Occludin in hyperosmolar stress and rh-TNF-α treated medium. TNF-α induced activation of MMPs was involved in the TJ disruption by ***:TJ proteins ZO-1 and Occludin are disrupted by hyperosmolar stress and TNF-α, but protected by MMP inhibitor(GM-6001). It suggests that TNF-α/MMP pathway mediates the TJ disruption in primary HCECs exposed to hyperosmotic stress.

关键词： dry eye hyperosmolarity cornea epithelium tight junction

Depth of tumor invasion and tumor-occupied portions of stomach are predictive factors of intra-abdominal metastasis

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Chinese Journal of Cancer Research 2017年第2期29卷 109-117页

作者： Ziyu li Zhemin li Shuqin Jia Zhaode Bu lianhai Zhang Xiaojiang Wu Shuangxi li Fei Shan xin Ji Jiafu Ji Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Center of Gastrointestinal Surgery Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Center for Molecular Diagnostics Peking University Cancer Hospital & Institute Beijing 100142 China

Objective： Diagnostic laparoscopy is recommended for the pretherapeutic staging of gastric cancer to detect any unexpected or unconfirmed intra-abdominal metastasis. The aim of this study was to evaluate the role and indications of diagnostic laparoscopy in the detection of intra-abdominal metastasis. Methods： Standard diagnostic laparoscopy with peritoneal cytology examination was performed prospectively on patients who were clinically diagnosed with primary local advanced gastric cancer （CT≥2M0）. We calculated the rate of intra-abdominal metastases identified by diagnostic laparoscopy, and examined the relationship between peritoneal dissemination （P） and cytology results （CY）. Split-sample method was applied to find clinical risk factors for intra-abdominal metastasis. Multivariate logistic regression analysis and receiver-operator characteristic （ROC） analysis were performed in training set to find out risk factors ofintra-abdominal metastasis, and then validate it in testing set. Results： Out of 249 cM0 patients, 51 （20.5%） patients with intra-abdominal metastasis were identified by diagnostic laparoscopy, including 20 （8.0%） P1CY1, 17 （6.8%） POCY1 and 14 （5.6%） P1CY0 patients. In the training set, multivariate logistic regression analysis and ROC analysis showed that the depth of tumor invasion on computer tomography （CT） scan ≥21 mm and tumor-occupied 〉2 portions of stomach are predictive factors of metastasis. In the testing set, when diagnostic laparoscopy was performed on patients who had one or two of these risk factors, the sensitivity and positive predictive value for detecting intra-abdominal metastasis were 90.0% and 32.1%, respectively. Conclusions： According to our results, depth of tumor invasion and tumor-occupied portions of stomach are predictive factors ofintra-abdominal metastasis.

关键词： Gastric cancer diagnostic laparoscopy peritoneal dissemination intra-peritoneal free cancer cells pretherapeutic staging

Silence of STIM1 attenua=tes the proliferation and migration of EPCs after vascular injury and its mechanism

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Asian Pacific Journal of Tropical Medicine 2014年第5期7卷 373-377页

作者： xin-Peng Cong Wen-Hui Wang Xi Zhu Can Jin liang liu xin-Min li Affiliated Shanghai East Hospital of Tongji University Shanghai Zhoupu Hospital

Objective:To investigate the effect of stromal interaction molecule 1（STIM1） knockdown on the proliferation and migration of endothelial progenitor cells（EPCs） after vascular injury and its ***:The rat bone marrow derived EPCs were divided into three groups:adenovirus negative control（group NSC）,rat STIM1 adenovirus vector transfection group（group si/rSTIM1） and rat &human recombinant STIM1 adenovirus transfection group（group si/rSTlM1+hSTIM1）.The STIM1 expressions in each group were detected by reverse transcription PCR after transfection;the cell proliferation was tested by[3H]thymidine incorporation assay（3HTdR）;Cell cycle was analyzed by flow cytometry;the cells’ migration activity was detected by Boyden assay;Calcium ion concentration was detected by using laser confocal ***:48 h later after transfection,the expression level of STIM1 in si/rSTIM1 cells was significantly lower than that in NSC group（0.21±0.12 vs 1.01±0.01,P1 phase in si/rSTIM1 group[（93.3l±0.24）%]were significantly more than that in NSC group[（78.03±0.34）%,Pliferation and migration after vascular injury,by mediating the calcium ion concentration in EPCs.

关键词： Vascular injury STIM1 Proliferation Migration tEPCs

Genome-wide identification of NAC gene family and expression analysis under abiotic stresses in Salvia miltiorrhiza

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BIOCELL 2022年第8期46卷 1947-1958页

作者： xin li JIANMIN PAN FAISAL ISLAM JUANJUAN li ZHUONI HOU ZONGQI YANG liNG XU Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou310018China Institute of Crop Science Ministry of Agriculture and Rural Affairs Laboratory of Spectroscopy SensingZhejiang UniversityHangzhou310058China

NAC(NAM,ATAF,CUC)is a class of transcription factors involved in plant growth regulation,abiotic stress responses,morphogenesis and *** miltiorrhiza is an important Chinese medicinal herb,but the characterization of NAC genes in this species is *** this study,based on the Salvia miltiorrhiza genomic databases,82 NAC transcription factors were identified,which were divided into 14 ***,phylogenetic analysis,gene structure,chromosomal localization and potential role of SmNACs in abiotic stress conditions were also *** results revealed that some SmNACs had different structures than others,which advised that these genes may have multiple/distinct ***-time quantitative polymerase chain reaction(RT-qPCR)analysis showed that SmNACs exhibited differential expression patterns under salt and drought *** NaCl induced salinity treatments modulated the expression of several SmNAC genes more in roots compared with ***,under drought stress conditions,more genes were upregulated in leaves compared with *** results will be useful for the further study involved in the functional characteristics of SmNAC genes,especially in response to salt and drought stresses,thereby may facilitate genetic breeding in Salvia miltiorrhiza.

关键词： Salvia miltiorrhiza Arabidopsis thaliana NAC transcription factors Genome-wide analysis Abiotic stress

Theoretical Study of Na Clusters by First Principle Molecular Dynamics Calculations

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Chinese Physics Letters 1999年第4期16卷 262-263页

作者： SU Chang-rong ZHU Jie li Jia-ming YANG Jin-long Department of Physics Center of Atomic and Molecular Sciences Tsinghua UniversityBeijing 100084 Institute of Physics Chinese Academy of SciencesBeijing 100080 Center of fundmental Physics University of Science and Technology of ChinaHefei 230026

Based on molecular dynamics simulations with first-principle forces,we have studied the Na *** with available high-precision experimental data(e.g.,geometric structure of Na_(3)),we then can assess validity of various first-principle theoretical *** first-principle molecular dynamics method is a powerful tool to study properties of ***,we can elucidate evolution of growth of Na clusters.

关键词： methods. validity dynamics

Carbon material-based anodes in the microbial fuel cells

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Carbon Energy 2021年第3期3卷 449-472页

作者： Xiaoqi Fan Yun Zhou Xueke Jin rong-Bin Song Zhaohui li Qichun Zhang Henan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical Applications School of Ecology and EnvironmentZhengzhou UniversityZhengzhouChina Department of Materials Science and Engineering City University of Hong KongKowloonHong KongChina

For the performance improvement of microbial fuel cells(MFCs),the anode becomes a breakthrough point due to its influence on bacterial attachment and extracellular electron transfer(EET).On other level,carbon materials possess the following features:low cost,rich natural abundance,good thermal and chemical stability,as well as tunable surface properties and spatial ***,the development of carbon materials and carbon-based composites has flourished in the anode of MFCs during the past *** this review,the major carbon materials used to decorate MFC anodes have been systematically summarized,based on the differences in composition and ***,we have also outlined the carbon material-based hybrid biofilms and carbon material-modified exoelectrogens in MFCs,along with the discussion of known strategies and mechanisms to enhance the bacteria-hosting capabilities of carbon material-based anodes,EET efficiencies,and MFC ***,the main challenges coupled with some exploratory proposals are also expounded for providing some guidance on the future development of carbon material-based anodes in MFCs.

关键词： carbon materials cell surface modification extracellular electron transfer hybrid biofilm microbial fuel cells

Fabry Disease with Aseptic Meningitis:A Case Series and literature Review of an Underestimated Clinical Presentation

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Current Medical Science 2022年第2期42卷 274-279页

作者： Ming-yu TANG Yue-hui HONG li-xin ZHOU Jun NI Department of Neurology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100005China

Objective Fabry disease(FD)is an X-linked lysosomal storage disease caused by the mutation in theα-galactosidase A gene that leads to a consequently decreasedα-galactosidase A enzyme activity and a series of clinical ***,FD accompanied with aseptic meningitis can be relatively scarce and rarely reported,which leads to significant clinical misdiagnosis of this *** Sixteen patients diagnosed with FD based on a decreased activity ofα-galactosidase A enzyme and/or genetic screening were identified through a 6-year retrospective chart review of a tertiary *** presentations,brain magnetic resonance imaging,cerebrospinal fluid analysis,treatment and outcome data were analyzed in cases of aseptic meningitis associated with *** Three out of 16 cases exhibited aseptic meningitis associated with *** was one female and two male patients with a mean age of 33.3 years.A family history of renal failure or hypertrophic cardiomyopathy was found in 3 *** cases presented with a persistent or intermittent headache and recurrent ischemic *** cerebrospinal fluid analyses showed mild pleocytosis in 2 patients and an elevated level of protein in all *** fluid cytology revealed activated lymphocytes,suggesting the existence of aseptic *** the literature review,up to 9 cases presenting with FD and aseptic meningitis were found,which bore a resemblance to our patients in demographic and clinical *** Our cases suggested that aseptic meningitis in FD might be under-detected and easily misdiagnosed,and should be more thoroughly examined in further cases.

关键词： Fabry disease aseptic meningitis ischemic stroke headache case series

Ferroelectric polarization, domains structures and magnetic property of Y_(1-x)In_xMnO_3

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Chinese Science Bulletin 2014年第36期59卷 5194-5198页

作者： li Wang Fu-Kuo Chiang Jun li Chao Ma Huai-xin Yang Jian-Qi li Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences

The vortex domains, structural properties and ferroelectric polarization in Y1-xinxMn O3 with 0 B x B 0.6have been extensively investigated in well-characterized samples. X-ray diffraction measurements demonstrated that the lattice parameters change continuously following the substitution of In for Y. Measurements of magnetic susceptibilities revealed that In substitution could visibly affect the magnetic transition and low-temperature magnetic properties. Transmission electron microscopy study showed that In substitution could result in notable decrease of the size of ferroelectric vortex domains. Cs-corrected scanning transmission electron microscopy observations and our careful analysis on atomic-poling configurations demonstrate that the ferroelectric polarizations of Y1-xinxMn O3 are suppressed with the increase of In content.

关键词：结构性能铁电极磁性透射电子显微镜电子显微镜观察 X射线衍射晶格参数磁化率

Preoperative gamma-glutamyltransferase to lymphocyte ratio predicts long-term outcomes in intrahepatic cholangiocarcinoma patients following hepatic resection

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World Journal of Gastroenterology 2020年第13期26卷 1501-1512页

作者： Jin-Ju Wang Hui li Jia-xin li lin Xu Hong Wu Yong Zeng Department of liver Surgery and liver Transplantation Center West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Laboratory of liver Surgery West China HospitalSichuan UniversityChengdu 610065Sichuan ProvinceChina

BACKGROUND Intrahepatic cholangiocarcinoma(ICC)is a heterogeneous hepatobiliary cancer with limited treatment options.A number of studies have illuminated the relationship between inflammation-based prognostic scores and outcomes in patients with ***,the use of reliable and personalized prognostic algorithms in ICC after resection is *** To assess the prognostic value of the gamma-glutamyltransferase to lymphocyte ratio(GLR)in ICC patients following curative *** ICC patients following curative resection(2009-2017)were divided into two cohorts:The derivation cohort and validation *** derivation cohort was used to explore an optimal cut-off value,and the validation cohort was used to further evaluate the *** survival(OS)and recurrence-free survival(RFS)were analyzed,and predictors of OS and RFS were *** A total of 527 ICC patients were included and randomly divided into the derivation cohort(264 patients)and the validation cohort(263 patients).The two patient cohorts had comparable baseline *** optimal cut-off value for the GLR was ***-Meier curves showed worse OS and RFS in the GLR>33.7 group compared with GLR≤33.7 group in both *** univariate and multivariate analysis,the results indicated that GLR was an independent prognostic factor of OS[derivation cohort:hazard ratio(HR)=1.620,95%confidence interval(CI):1.066-2.462,P=0.024;validation cohort:HR=1.466,95%CI:1.033-2.142,P=0.048]and RFS[derivation cohort:HR=1.471,95%CI:1.029-2.103,P=0.034;validation cohort:HR=1.480,95%CI:1.057-2.070,P=0.022].CONCLUSION The preoperative GLR is an independent prognostic factor for ICC patients following hepatectomy.A high preoperative GLR is associated with worse OS and RFS.

关键词： Gamma-glutamyltransferase Lymphocyte ratio Gamma-glutamyltransferase to lymphocyte ratio Intrahepatic cholangiocarcinoma Prognosis Survival analysis