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Kinematics of whip spider pedipalps: a 3D comparative morpho-functional approach

Integrative Zoology 2022年第1期17卷 156-167页

作者： Michel SCHMIDT roland r.melzer russell D.C.BICKNELL Bavarian State Collection of Zoology Bavarian Natural History CollectionsMunichGermany Department Biology II Ludwig-Maximilians-Universität MünchenMunichGermany GeoBio-Center Ludwig-Maximilians-Universität MünchenMunichGermany Palaeoscience research Centre School of Environmental and Rural ScienceUniversity of New EnglandArmidaleAustralia

Amblypygi are tropical and subtropical ambush predators that use elongated,raptorial pedipalps for different *** pedipalp use in predation and courtship has been explored in videography in vivo analyses,kinematic ex vivo examination of these appendages has not been ***,we rectify this lack of data by using microCT scans to 3D-kinematically model the appendage morphology and the range of motion(rOM)of the joints for Damon medius and Heterophrynus *** illustrate the successful application of this technique to terrestrial euarthropods in determining the maximum rOM values for each pedipalp *** also note that,in life,these values would be lower due to motion restricting structures like tendons,arthrodial membranes,and *** further compare our maximum values obtained here with data from video-based motion *** rOM of each joint shows the greatest flexibility in the femur-tibia joint(140–150°),the lowest in the basitarsus-claw joint(35–40°).rOM in the tibia-basitarsus joint is markedly distinct(***:44°;***:105°).This disparity reflects how *** uses the joint in the capture basket,while *** uses the femur-tibia joint to form the capture *** further illustrate notable vertical motion of the *** pedipalp compared to *** difference reflects the retro-ventral trochanter apophysis of *** study opens the possibility to further whip spider kinematic *** of other taxa using this approach will result in a more comprehensive understanding of the ecological significance and ethological implications of this unique arachnid group.

关键词： 3D kinematic analyses Amblypygi arthropod joints Autodesk Maya Damon medius Heterophrynus elaphus range of motion analyses

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Evaluation of Mandibular Deprogramming Using TENS (Transcutaneous Electrical Nerve Stimulation) in Patients at the Specialty Clinic of the Juarez Autonomous University of Tabasco from 2022 to 2024

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Journal of Biosciences and Medicines 2024年第7期12卷 245-251页

作者： Christian C. Aguilar-Caamal Landy V. Limonchi-Palacio Crystell G. Guzmán-Priego Jeannette ramírez-Mendoza José M. Lehmann-Mendoza Miguel A. López-Alvarado Xavier E. Moreno-Enriquez Jorda A. Albarrán-melzer Academic Division of Health Sciences Juarez Autonomous University of Tabasco Villahermosa Tabasco Mxico Cardiometabolism Laboratory Academic Division of Health Sciences Juarez Autonomous University of Tabasco Villahermosa Tabasco Mxico

Temporomandibular disorders (TMD) negatively affect quality of life, causing pain and restricted jaw movements. This study evaluates the effectiveness of Transcutaneous Electrical Nerve Stimulation (TENS) on TMD patients before orthodontic treatment at the Juarez Autonomous University of Tabasco. A quantitative, quasi-experimental study was conducted with 30 patients who met the inclusion criteria: aged 18 to 65 years, with TMJ pain symptoms, diagnosed with temporomandibular disorders using the Helkimo index, and who had provided informed consent for the study. The range of mandibular movement and the Visual Analog Scale (VAS) were measured to record values before therapy. The electrodes of the TENS therapy were positioned on both sides of the jaw in the preauricular area and the masseter muscle, the therapy was administered for 40 minutes, utilizing a frequency of 2 - 4 Hz and a pulse duration of 300 microseconds. Following this, the range of mandibular movement and the VAS were reassessed to evaluate the outcomes., significantly improved maximum mouth opening (37.7 ± 6.4 mm to 44.6 ± 5.88 mm), right laterality (3.86 ± 1.57 mm to 5.43 ± 1.21 mm), left laterality (3.13 ± 1.63 mm to 4.53 ± 1.61 mm), protrusion (4.01 ± 1.7 mm to 5.36 ± 1.32 mm), and pain perception (4.56 ± 2.045 to 2.03 ± 1.5) (P rapy enhances mandibular movement and reduces pain, making it a well-tolerated, non-invasive complementary treatment for TMD.

关键词： TENS Opening Deprogramming Electrostimulation TMJ (Temporomandibular Joint)

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Immunoelectron Microscopy for Locating Calvin Cycle Enzymes in the Thylakoids of Synechocystis 6803

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Molecular Plant 2009年第1期2卷 32-42页

作者： rachna Agarwal Stefan Ortleb layashree Krishna Sainis Michael melzer Molecular Biology Division Bhabha Atomic Research Center Mumbai 400085 India Structural Cell Biology Group Molecular Cell Biology Department Leibniz Institute of Plant Genetics and Crop Plant Research Correnstr. 3 Gatersleben06466 Germany

Unicellular cyanobacteria Synechocystis 6803 were fixed using high-pressure freezing （HPF） and freeze substitution without any chemical cross-linkers. Immunoelectron microscopy of these cells showed that five sequential enzymes of the Calvin cycle （phosphoriboisomerase, phosphoribulokinase, ribulose-1,5-bisphosphate carboxylase/oxygenase （ruBisCO）, 3-phosphoglyceratekinase and glyceraldehyde-3-phosphate dehydrogenase） and the catalytic portion of the chloroplast H^＋-ATP synthase （CF1） are located adjacent to the thylakoid membranes. Cell-free extracts of Synechocystis were processed by ultracentrifugation to isolate thylakoid fractions sedimenting at 40 000, 90 000, and 150 000 g. Among these, the 150 000-g fraction showed the highest linked activity of the above five sequential Calvin cycle enzymes and also the highest coordinated activity of light and dark reactions as assessed by ribose-5-phosphate （r-5-P）＋ADP dependent CO2 fixation. Immunogold labeling of this membrane fraction confirmed the presence of the above five enzymes as well as the catalytic portion of the CF1 ATP synthase. Notably, the protein A-gold labeling of the thylakoids was observed without use of chemical cross-linkers and in spite of the normal washing steps used during standard immunolabeling. The results showed that soluble Calvin cycle enzymes might be organized along the thylakoid membranes.

关键词： High-pressure freezing immunogold labeling membrane-isolation Synechocystis thylakoids.

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Amending a tropical Arenosol:increasing shares of biochar and clay improve the nutrient sorption capacity

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Biochar 2022年第1期4卷 936-958页

作者： Christine Beusch Dennis melzer Arne Cierjacks Martin Kaupenjohann Institute of Ecology Chair of Soil ScienceTechnische Universität BerlinErnst-Reuter-Platz 110587 BerlinGermany Institute of Agricultural and Urban Ecological Projects(IASP) Affiliated to Berlin Humboldt UniversityPhilippstraße 13Haus 1610115 BerlinGermany Institute of Ecology Chair of Ecosystem Science/Plant EcologyTechnische Universität BerlinRothenburgstr.1212165 BerlinGermany Department of Landscape Development/Vegetation Technology University of Applied Sciences DresdenPillnitzer Platz 201326 DresdenGermany

Tropical Arenosols may be challenging for agricultural use,particularly in semi-arid *** aim of this study was to evaluate the impact of the addition of increasing shares of biochar and clay on the nutrient sorption capacity of a tropical *** batch equilibrium experiments,the sorption of ammonium-N(NH_(4)^(+)-N),nitrate-N(NO_(3)^(−)-N),potassium(K^(+)),and phosphate-P(PO_(4)^(3−)-P)was quantified for mixtures of an Arenosol with increasing shares of biochar and clay(1%,2.5%,5%,10%,100%)and the unmixed Arenosol,biochar,and *** mid-temperature biochar was produced from Prosopis juliflora feedstock;the clayey material was taken from the sedimentary parent material of a temporarily dry *** the Arenosol-biochar mixture with 10%biochar addition and the biochar increased the NH_(4)^(+)-N maximum sorption capacity(q_(max))of the Arenosol,by 34%and 130%,*** q_(max)of PO_(4)^(3−)-P slightly increased with ascending biochar shares(1-10%)by 14%,30%,26%,and 42%,whereas the undiluted biochar released PO_(4)^(3−)-*** addition slightly reduced NO_(3)^(−)-N release from the Arenosol but strongly induced K^(+)*** the other hand,clay addition of 10%and clay itself augmented q_(max)of NH_(4)^(+)-N by 30%and 162%;ascending clay rates(1-100%)increased q_(max)for PO_(4)^(3−)-P by 78%,130%,180%,268%,and 712%.Clay rates above 5%improved K^(+)sorption;however,no q_(max)values could be *** of NO_(3)^(−)-N remained unaffected by clay ***,clay addition proved to enhance the nutrient sorption capacity of the Arenosol more effectively than biochar;nonetheless,both materials may be promising amendments to meliorate sandy soils for agricultural use in the semi-arid tropics.

关键词： Coarse-textured soils Biochar Clay Batch equilibrium experiment Sorption isotherms

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Assembly of multicomp on ent structures from hundreds of micron-scale building blocks using optical tweezers

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Microsystems & Nanoengineering 2021年第3期7卷 163-171页

作者： Jeffrey E.melzer Euan McLeod Wyant College of Optical Sciences The University of ArizonaTucsonArizona85721USA

The fabrication of three-dimensional(3D)microscale structures is critical for many applications,including strong and lightweight material development,medical device fabrication,microrobotics,and photonic *** 3D microfabrication has seen progress over the past decades,complex multicomponent integration with small or hierarchical feature sizes is still a *** this study,an optical positioning and linking(OPAL)platform based on optical tweezers is used to precisely fabricate 3D microstructures from two types of micron-scale building blocks linked by biochemical interactions.A computer-controlled interface with rapid on-the-fly automated recalibration routines main tains accuracy eve n after placi ng many buildi ng *** achieves a 60-nm positional accuracy by optimizing the molecular functionalization and laser power.A two-component structure consisting of 4481-pm building blocks is assembled,representing the largest number of building blocks used to date in 3D optical tweezer *** optical tweezers have previously been used for microfabrication,those results were generally restricted to single-material structures composed of a relatively small number of larger-sized building blocks,with little discussion of critical process *** is anticipated that OPAL will enable the assembly,augmentation,and repair of microstructures composed of specialty micro/nanomaterial building blocks to be used in new photonic,microfluidic,and biomedical devices.

关键词： hundreds blocks sized

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Human microbiome: From the bathroom to the bedside

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World Journal of Gastrointestinal Pathophysiology 2015年第3期6卷 79-85页

作者： Stephen Malnick Ehud melzer Department of Internal Medicine C Kaplan Medical CenterAffiliated to the Hebrew University Institute of Gastroenterology and Liver Disease Kaplan Medical CenterAffiliated to the Hebrew University

The human gut contains trillions of bacteria, the major phylae of which include Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria. Fecal microbial transplantation(FMT) has been known of for manyyears but only recently has been subjected to rigorous examination. We review the evidence regarding FMT for recurrent Clostridium difficile infection which has resulted in it being an approved treatment. In addition there is some evidence for its use in both irritable bowel syndrome and inflammatory bowel disease. Further research is needed in order to define the indications for FMT and the most appropriate method of administration.

关键词： Fecal microbial transplant Clostridium difficile Side-effects Indications Metabolic disorders

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reduced striatal volumes in Parkinson’s disease:a magnetic resonance imaging study

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Translational Neurodegeneration 2012年第1期1卷 132-139页

作者： Toni L Pitcher Tracy r.melzer Michael r MacAskill Charlotte F Graham Leslie Livingston ross J Keenan richard Watts John C Dalrymple-Alford Tim J Anderson Department of Medicine University of OtagoChristchurchNew Zealand New Zealand Brain research Institute 66 Stewart StChristchurch 8011New Zealand Department of Psychology University of CanterburyChristchurchNew Zealand College of Medicine University of VermontBurlingtonVTUSA Christchurch radiology Group ChristchurchNew Zealand Department of Neurology Canterbury District Health BoardChristchurchNew Zealand

Background:The presence and extent of structural changes in the brain as a consequence of Parkinson’s disease(PD)is still poorly ***:High-resolution 3-tesla T1-weighted structural magnetic resonance images in sixty-five PD and 27 age-matched healthy control participants were ***,caudate,and intracranial volumes were manually traced in the axial plane of 3D reconstructed *** nuclei volumes were normalized to intracranial volume for statistical *** status was assessed using the Unified Parkinson’s Disease rating Scale and Hoehn and Yahr *** status was assessed using global status tests and detailed neuropsychological ***:Both caudate and putamen volumes were smaller in PD brains compared to controls after adjusting for age and *** volumes were reduced by 11%(p=0.001)and putamen volumes by 8.1%(p=0.025).PD striatal volumes were not found to be significantly correlated with cognitive or motor ***:Small,but significant reductions in the volume of both the caudate and putamen occur in PD *** reductions are independent of the effects of age and gender,however the relation of these reductions to the functional loss of dopamine,which is characteristic of PD,remains unclear.

关键词： Magnetic resonance imaging Volumetry Caudate Putamen Parkinson’s disease

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PDX1.1-dependent biosynthesis of vitamin B6 protects roots from ammonium-induced oxidative stress

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Molecular Plant 2022年第5期15卷 820-839页

作者： Ying Liu rodolfo A.Maniero ricardo F.H.Giehl Michael melzer Priscille Steensma Gabriel Krouk Teresa B.Fitzpatrick Nicolaus von Wirén Molecular Plant Nutrition Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Corrensstrasse 306466 GaterslebenGermany Structural Cell Biology Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Corrensstrasse 306466 GaterslebenGermany Department of Botany and Plant Biology University of Geneva1211 GenevaSwitzerland BPMP Universitéde MontpellierCNRSINRASupAgroMontpellierFrance State Key Laboratory of Crop Genetics and Germplasm Enhancement College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjing 210095China

Despite serving as a major inorganic nitrogen source for plants,ammonium causes toxicity at elevated con-centrations,inhibiting root elongation early *** previous studies have shown that ammonium-inhibited root development relates to ammonium uptake and formation of reactive oxygen species(rOS)in roots,it remains unclear about the mechanisms underlying the repression of root growth and how plants cope with this inhibitory effect of *** this study,we demonstrate that ammonium-induced apo-plastic acidification co-localizes with Fe precipitation and hydrogen peroxide(H_(2)O_(2))accumulation along the stele of the elongation and differentiation zone in root tips,indicating Fe-dependent rOS *** screening ammonium sensitivity in T-DNA insertion lines of ammonium-responsive genes,we identified PDX1.1,which is upregulated by ammonium in the root stele and whose product catalyzes de novo biosyn-thesis of vitamin *** growth of pdx1.1 mutants is hypersensitive to ammonium,while chemical complementation or overexpression of PDX1.1 restores root *** salvage strategy requires non-phosphorylated forms of vitamin B6 that are able to quench rOS and rescue root growth from ammo-nium ***,these results suggest that PDX1.1-mediated synthesis of non-phosphorylated B6 vitamers acts as a primary strategy to protect roots from ammonium-dependent rOS formation.

关键词： ammonium nutrition apoplastic pH Fe mobilization root elongation pyridoxine rOS scavenging

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Five years of fecal microbiota transplantation-an update of the Israeli experience

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World Journal of Gastroenterology 2018年第47期24卷 5403-5414页

作者： Sharon A Greenberg Ilan Youngster Nathaniel A Cohen Dan M Livovsky Jacob Strahilevitz Eran Israeli Ehud melzer Kalman Paz Naomi Fliss-Isakov Nitsan Maharshak Department of Gastroenterology and Liver Diseases Tel Aviv Sourasky Medical Centeraffiliated with the Sackler Faculty of MedicineTel Aviv UniversityTel Aviv 6423906Israel Bacteriotherapy Clinic Tel Aviv Sourasky Medical Centeraffiliated with the Sackler Faculty of MedicineTel Aviv UniversityTel Aviv 6423906Israel Assaf Harofe Medical Center Zerifin 70300Israel Digestive Diseases Institute Shaare Zedek Medical CenterJerusalem 91031Israel Department of Clinical Microbiology and Infectious Diseases Hadassah-Hebrew UniversityJerusalem 91120Israel Department of Gastroenterology and Liver Diseases Hadassah-Hebrew UniversityJerusalem 91120Israel Gastrointestinal and Liver Diseases Institute Kaplan Medical CenterRehovot 76100Israel

AIM To evaluate and describe the efficacy of fecal microbiota transplantation(FMT) for Clostridium difficile infection(CDI) in a national Israeli *** All patients who received FMT for recurrent(recurrence within 8 wk of the previous treatment) or refractory CDI from 2013 through 2017 in all the five medical centers in Israel currently performing FMT were included. Stool donors were screened according to the Israeli Ministry of Health guidelines. Clinical and laboratory data of patients were collected from patients' medical files, and they included indications for FMT, risk factors for CDI and disease severity. Primary outcome was FMT success(at least 2 mo free of CDI-related diarrhea post-FMT). Secondary outcomes included initial response to FMT(cessation of diarrhea within 7 d) and recurrence at 6 *** There were 111 FMTs for CDI, with a median age of 70 years [interquartile range(IQr): 53-82], and 42%(47) males. Fifty patients(45%) were treated via the lower gastrointestinal(LGI, represented only by colonoscopy) route, 37(33%) via capsules, and 24(22%) via the upper gastrointestinal(UGI) route. The overall success rate was 87.4%(97 patients), with no significant difference between routes of administration(P = 0.338). In the univariant analysis, FMT success correlated with milder disease(P = 0.01), ambulatory setting(P r Charlson comorbidity score(P riant analysis, only severe CDI [odd ratio(Or) = 0.14, P r = 0.19, P re each independently inversely related to FMT success. There were 35(32%) patients younger than 60 years of age, and 14(40%) of them had a background of inflammatory bowel *** FMT is a safe and effective treatment for CDI, with capsules emerging as a successful and well-tolerated route. Severe CDI is less likely to respond to FMT.

关键词： Clostridium difficile infection Capsules Israel Fecal microbiota transplantation

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高通量透析膜蛋白质通透性的研究

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中华肾脏病杂志 2005年第9期21卷 548-551页

作者：徐筱琪 Siegfried Stiller Helmut Mann 钱家麒 Heinrich melzer 上海第二医科大学附属仁济医院肾脏科 200001 德国亚琛透析中心

目的评估高通量透析膜的蛋白质通透性。方法所有的透析模式均设为单纯超滤,在应用不同的透析膜透析时于透析器的出液口留取滤出液标本,同时进行蛋白质浓度测定和十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SDS-PAGE)作蛋白质电泳分析。SDS-PAG... 详细信息

目的评估高通量透析膜的蛋白质通透性。方法所有的透析模式均设为单纯超滤,在应用不同的透析膜透析时于透析器的出液口留取滤出液标本,同时进行蛋白质浓度测定和十二烷基硫酸钠-聚丙烯酰氨凝胶电泳(SDS-PAGE)作蛋白质电泳分析。SDS-PAGE采用银染色的方法并根据melzer的方法作了改良,结果应用激光吸光度测定仪进行条带分析。结果透析膜滤出液中蛋白质条带与经肾小球膜滤出的蛋白质相似,含IgG、转铁蛋白、视黄醇结合蛋白和β2-微球蛋白。由于不同的透析膜的分子截留量、电荷和吸附能力不同,其蛋白质条带的分布亦不同。随着透析的进行,所有膜的通透性都逐渐下降。结论高通量透析膜的蛋白质通透性与肾小球滤过膜相似,但受孔径、表面所带电荷、吸附能力和透析时间的影响而发生改变。与人体的肾小球滤过膜不同,随着透析的进行,透析膜的蛋白质通透性下降。

关键词：膜,人工通透性血液透析电泳,聚丙烯酰氨凝胶高通量高通量透析膜蛋白质 SDS-PAGE 肾小球滤过膜十二烷基硫酸钠

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