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Microengineering double layer hydrogel structures towards the recapitulation of the hematopoietic stem cell niche

Science Bulletin 2018年第20期63卷 1319-1323页

作者： Pilar Carreras rodrigo cortrim chaves Miguel Gallardo Alejandra Ortiz Joaquin Martinez Lopez Samuel K.Sia Department of Biomedical Engineering Columbia UniversityNew YorkNY 10032USA Hematology Department Research Institute i+12Hospital 12 OctubreMadrid 2804Spain Hematological Malignancies Clinical Research Unit H120 CNIOMadrid 28029Spain

Adult stem cells,especially from bone marrow,have a tremendous therapeutic potential,and its application in regenerative medicine has been increasingly studied [1,2].It is well known that three-dimensional (3D)cell culture matrix promotes many biological relevant functions not observed in two-dimensional (2D) monolayer cell culture [3]and therefore,engineering 3D cell culture platforms to mimic the native in vivo biological systems represents a realistic approach for studies of bone marrow stem cells. High-throughput microfluidics constitutes a promising technology to generate hydrogel three dimensional constructs [4,5],however, its application to host adult stem cells remains still a challenge.

关键词： hydrogel hematopoietic stem cell

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Sm-Nd systematics of metaultramafic-mafic rocks from the Arroio Grande Ophiolite(Brazil): Insights on the evolution of the South Adamastor paleo-ocean

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Geoscience Frontiers 2020年第6期11卷 2287-2296页

作者： rodrigo chaves Ramos Edinei Koester Daniel Triboli Vieira Programa de Pòs-Graduação Em Geociências Instituto de GeociênciasUniversidade Federal Do Rio Grande Do Sul.PO Box 1500191501-970Porto AlegreRSBrazil Departamento de Geologia Universidade Federal Do Rio Grande Do Sul.Bento Gonçalves Avenue950091540-000Porto AlegreRSBrazil

In this contribution we present new insights on the evolution of the Dom Feliciano Belt,southernmost Mantiqueira Province,integrating new whole-rock Sm-Nd isotopic data for the Arroio Grande Ophiolite(Punta del Este Terrane,Brazil/Uruguay border)with previously published bulk-rock and isotope geochemistry of the South Adamastor paleo-ocean metamafic rocks located in Uruguay(Paso del Dragon Complex,Punta del Este Terrane)and Namibia(Chameis Subterrane,Marmora Terrane,Gariep Belt).For the regional geology,the new data corroborate previous hypothesis and demonstrate the depleted mantle features of the amphibolites and metagabbros of the studied *** Arroio Grande Ophiolite rocks are compared with its Uruguayan and Namibian counterparts,demonstrating their isotopic and geochemical similarities and differences,and the backarc affinity of the South Adamastor *** MORB-affinity amphibolites from the Arroio Grande Ophiolite-Paso del Dragon Complex are,so far,the most juvenile rocks in the eastern sector of the Dom Feliciano Belt,yielding εNd(640)Ma)between+7.3 and+9,and high 147Sm/144Nd(>0.169)and 143Nd/144Nd(640 Ma)ratios(0.51219-0.51229).The South Adamastor is interpreted in this paper as an internal back-arc ocean,with limited lateral extension,opened at around 750-650 Ma as the result of the closure of the older Charrua-Goianide paleoocean during the Brasiliano/Pan-African orogenic cycle and final configuration of the West Gondwana paleocontinent.

关键词： West Gondwana Brasiliano orogenic cycle Adamastor ocean

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Alkaline activation of endogenous latent TGFβ1 by an injectable hydrogel directs cell homing for in situ complex tissue regeneration

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Bioactive Materials 2022年第9期7卷 316-329页

作者： Sainan Wang Yuting Niu Peipei Jia Zheting Liao Weimin Guo rodrigo Cotrim chaves Khanh-Hoa Tran-Ba Ling He Hanying Bai Sam Sia Laura J.Kaufman Xiaoyan Wang Yongsheng Zhou Yanmei Dong Jeremy J.Mao Department of Cariology and Endodontology Peking University School and Hospital of StomatologyBeijing100081China Department of Prosthodontics Peking University School and Hospital of StomatologyBeijing100081China Center for Craniofacial Regeneration Columbia UniversityNew YorkNY10032USA Department of Biomedical Engineering Columbia UniversityNew YorkNY10027USA Department of Chemistry Columbia UniversityNew YorkNY10027USA

Utilization of the body’s regenerative potential for tissue repair is known as in situ tissue ***,the use of exogenous growth factors requires delicate control of the dose and delivery strategies and may be accompanied by safety,efficacy and cost *** this study,we developed,for the first time,a biomaterial-based strategy to activate endogenous transforming growth factor beta 1(TGFβ1)under alkaline conditions for effective in situ tissue *** demonstrated that alkaline-activated TGFβ1 from blood serum,bone marrow fluids and soaking solutions of meniscus and tooth dentin was capable of increasing cell recruitment and early differentiation,implying its broad ***,we engineered an injectable hydrogel(MS-Gel)consisting of gelatin microspheres for loading strong alkaline substances and a modified gelatin matrix for hydrogel click *** vitro models showed that alkaline MS-Gel controllably and sustainably activated endogenous TGFβ1 from tooth dentin for robust bone marrow stem cell *** importantly,infusion of in vivo porcine prepared root canals with alkaline MS-Gel promoted significant pulp-dentin regeneration with neurovascular stroma and mineralized tissue by endogenous proliferative ***,this work offers a new bench-to-beside translation strategy using biomaterial-activated endogenous biomolecules to achieve in situ tissue regeneration without the need for cell or protein delivery.

关键词： Endogenous TGFβ1 Injectable alkaline hydrogel Cell homing Pulp-dentin complex In situ tissue regeneration

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An Online mtDNA Tool for Identification of Neotropical Psittacid Species and Taxonomic Issues:A Study Case of the Amazona ochrocephala Complex

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Natural Resources 2014年第11期5卷 634-652页

作者： Anderson Vieira chaves rodrigo Octavio de Paiva Queiroz-Filho Fabiano Augusto Assuncao Silva Cristina Yumi Miyaki Fabricio Rodrigues dos Santos Departamento de Biologia Geral Instituto de Ciencias Biologicas(ICB)Universidade Federal de Minas GeraisBelo HorizonteBrazil Policia Federal Belo HorizonteBrazil Departamento de Genetica e Biologia Evolutiva Instituto de BiocienciasUniversidade de Sao PauloSao PauloBrazil

Parrots are among the most popular pets in the world and they are also some of the most illegally traded, particularly in Brazil. Some computational tools were recently developed by researchers based on molecular databases for taxonomy support, forensic identification and conservation purposes. In this study, the DNA Surveillance platform was used to build an online database tool for molecular identification of Brazilian Psittacids using DNA sequences of six mitochondrial genes. To illustrate possible taxonomic issues of the online tool due to interspecific hybridization or unresolved taxonomy, we focused on Amazona aestiva that is considered as one of the most common parrots in Brazil, commonly bred as pets, and considered to be part of a species complex with Amazona ochrocephala from South America. We provide three curated sequence databases, which allow the species identification of individuals or tissue samples of birds of the Psittacidae family using mitochondrial DNA markers, and a comprehensive description of a taxonomic issue involving the A. ochrocephala complex. The results obtained corroborate previous studies suggesting that these species are not reciprocally monophyletic, due to either an ancient hybridization in central Brazil, or, they maybe just are morpho-varieties of the same species. Alternatively, if A. aestiva and A. ochrocephala were considered as sister species, the data could be interpreted either as a result of secondary contact or incipient speciation. Beyond the use of mtDNA for species identification, the high mtDNA haplotype diversity observed in A. aestiva indicates its potential use in discrimination of lineages that could be an important auxiliary tool to certify the captive origin of legally commercialized parrots.

关键词： Amazona aestiva Illegal Trade of Birds Psittaciformes Phylogeography DNA-Surveillance

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Evaluation of Intraobserver and Interobserver Reliability of Mechanical Axis Alignment Measure of the Lower Limb through the Panoramic Radiograph in Patients in the Preoperative and Postoperative Periods of Total Knee Arthroplasty

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Open Journal of Orthopedics 2020年第9期10卷 221-233页

作者： Murilo Barroso Matos José Leonardo Rocha de Faria Douglas Mello Pavão Márcio Liu Sandt Breno chaves de Almeida Pigozzo Pedro Guilme Teixeira de Sousa Filho rodrigo Pires e Albuquerque CLIFOR-Orthopedics and Fractures Clinic Vitória Brazil Knee Surgery Center National Institute of Traumatology and Orthopedics Rio de Janeiro Brazil Genutarso-Orthopedics Clinic Rio de Janeiro Brazil Genu Surgery Group Fortaleza Brazil

Objective: To evaluate intraobserver and interobserver reliability of the measurement of the mechanical axis of the lower limb by means of the panoramic radiograph of lower limbs by physicians with different levels of medical practice. Method: A retrospective cross-sectional study with preoperative and postoperative radiographic analysis of a group of 100 patients submitted to total knee arthroplasty. Results: It was observed that there is a highly significant intraobserver and interobserver agreement (p Conclusion: The panoramic radiograph of the lower limbs is a reliable method for measuring the mechanical axis of the lower limb in patients submitted to total knee arthroplasty, regardless of the level of medical practice of the evaluator.

关键词： Knee Mechanical Axis Total Knee Arthroplasty

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