Microengineering double layer hydrogel structures towards the recapitulation of the hematopoietic stem cell niche

作     者：Pilar Carreras Rodrigo Cortrim Chaves Miguel Gallardo Alejandra Ortiz Joaquin Martinez Lopez Samuel K.Sia 

作者机构：Department of Biomedical EngineeringColumbia UniversityNew YorkNY 10032USA Hematology DepartmentResearch Institute i+12Hospital 12 OctubreMadrid 2804Spain Hematological Malignancies Clinical Research Unit H120CNIOMadrid 28029Spain 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2018年第63卷第20期

页      面：1319-1323页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：supported by the European Commission H2020 Marie Curie Research Grants Scheme MSCA-IF-GF(705163) 

主　　题：hydrogel hematopoietic stem cell 

摘      要：Adult stem cells,especially from bone marrow,have a tremendous therapeutic potential,and its application in regenerative medicine has been increasingly studied [1,2].It is well known that three-dimensional (3D)cell culture matrix promotes many biological relevant functions not observed in two-dimensional (2D) monolayer cell culture [3]and therefore,engineering 3D cell culture platforms to mimic the native in vivo biological systems represents a realistic approach for studies of bone marrow stem cells. High-throughput microfluidics constitutes a promising technology to generate hydrogel three dimensional constructs [4,5],however, its application to host adult stem cells remains still a challenge.