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Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems

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Asian Pacific Journal of Tropical Biomedicine 2016年第8期6卷 682-685页

作者： William Patrick Cruiz Buhian Raquel Orejudos Rubio Demetrio Lim valle Jr. Juliana Janet Martin-Puzon Natural Sciences Research Institute University of the Philippines Institute of Biology University of the Philippines Department of Pathology and Laboratories Makati Medical Center

Objective: To determine the bioactive phytochemicals and antimicrobial activity of leaf and stem ethanolic extracts from Muntingia calabura L.(M. calabura).Methods: Dried leaves and stems of M. calabura were extracted with 95% ethanol. The antibacterial and antifungal activities of the extracts were examined using the disc diffusion assay. The minimum inhibitory concentration(MIC) of each extract showing antimicrobial activity was determined. The dried extracts were subjected to phytochemical screening to determine the presence of bioactive components. Total phenolic and flavonoid contents were also determined by the Folin–Ciocalteu method and the aluminum chloride method, ***: Varying degrees of antimicrobial activity were exhibited by the leaf and stem extracts against Pseudomonas aeruginosa(P. aeruginosa), Salmonella typhimurium,Staphylococcus aureus(S. aureus), Bacillus subtilis, and Candida albicans(C. albicans),with minimal activity against Escherichia coli. Based on the MIC, the extracts showed the highest activity against C. albicans, S. aureus and P. aeruginosa. Phytochemical screening revealed the presence of sterols, flavonoids, alkaloids, saponins, glycosides and tannins in the leaf extract; however, no triterpenes were detected. In the stem extract,triterpenes were detected along with relative amounts of flavonoids, saponins, glycosides and tannins. Alkaloids and sterols were absent in the stem ***: M. calabura leaf and stem ethanol extracts are potential sources of antibacterial agents against P. aeruginosa and S. aureus. This study reports for the first time the high degree of antifungal activity of M. calabura ethanolic extract, especially against C. albicans.

关键词： Antibacterial Antifungal Bioactive metabolites Disc diffusion Minimum inhibitory concentration Muntingia calabura

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Experimental Assessment of the Battery Lifetime in WSN Based on the Duty-CycleCurrentAverageMethod

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Wireless Sensor Network 2014年第10期6卷 212-220页

作者： Leonardo Barboni Maurizio valle Facultad de Ingenieria Universidad de la Republica Montevideo Uruguay Department of Naval Electrical Electronic and Telecommunications Engineering University of Genova Genova Italy

A great amount of work addressed methods for predicting the battery lifetime in wireless sensor systems. In spite of these efforts, the reported experimental results demonstrate that the duty-cycle current average method, which is widely used to this aim, fails in accurately estimating the battery life time of most of the presented wireless sensor system applications. The aim of this paper is to experimentally assess the duty-cycle current average method in order to give more effective insight on the effectiveness of the method. An electronic metering system, based on a dedicated PCB, has been designed and developed to experimentally measure node current consumption profiles and charge extracted from the battery in two selected case studies. A battery lifetime measurement (during 30 days) has been carried out. Experimental results have been assessed and compared with estimations given by using the duty-cycle current average method. Based on the measurement results, we show that the assumptions on which the method is based do not hold in real operating cases. The rationality of the duty-cycle current average method needs reconsidering.

关键词： Battery Lifetime Current Consumption TinyOS Measurement Duty-Cycle Current Average Method

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Incidence of hepatocellular carcinoma in patients with chronic liver disease due to hepatitis B or C and coinfected with the human immunodeficiency virus:a retrospective cohort study

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World Journal of Gastroenterology 2018年第5期24卷 613-622页

作者： Patrícia dos Santos Marcon Cristiane valle Tovo Dimas Alexandre Kliemann Patrícia Fisch Angelo Alves de Mattos Hepatology Post-Graduate Program Universidade Federal de Ciências da Saúde de Porto Alegre(UFCSPA)Porto Alegre 90020-090RSBrazil Infectology Department at Hospital Nossa Senhora da Conceicao Porto Alegre 91350-200RSBrazil Epidemiology Department at Hospital Nossa Senhora da Conceicao Porto Alegre 91350-200RSBrazil

AIM To assess the incidence of hepatocellular carcinoma(HCC) in chronic liver disease due to hepatitis B virus(HBV) or hepatitis C virus(HCV) coinfected with human immunodeficiency virus(HIV).METHODS A retrospective cohort study was performed, including patients with chronic liver disease due to HBV or HCV, with and without HIV coinfection. Patients were selected in the largest tertiary public hospital complex in southern Brazil between January 2007 and June 2014. We assessed demographic and clinical data, including lifestyle habits such as illicit drug use or alcohol abuse, in addition to frequency and reasons for hospital admissions via medical records *** Of 804 patients were included(399 with HIV coinfection and 405 monoinfected with HBV or HCV). Coinfected patients were younger(36.7 ± 10 vs 46.3 ± 12.5, P < 0.001). Liver cirrhosis was observed in 31.3% of HIV-negative patients and in 16.5% of coinfected(P < 0.001). HCC was diagnosed in 36 patients(10 HIV coinfected and 26 monoinfected). The incidence density of HCC in coinfected and monoinfected patients was 0.25 and 0.72 cases per 100 patient-years(95%CI: 0.12-0.46 vs 0.47-1.05)(long-rank P = 0.002), respectively. The ratio for the HCC incidence rate was 2.98 for HIV-negative. However, when adjusting for age or when only cirrhotic are analyzed, the absence of HIV lost statistical significance for the development of HCC. CONCLUSION In this study, the presence of HIV coinfection in chronic liver disease due to HBV or HCV showed no relation to the increase of HCC incidence.

关键词： Hepatocellular carcinoma Chronic hepatitis human immunodeficiency virus Coinfection Cirrhosis

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Effect of Molasses Addition at Ensiling on Ruminal In Situ Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages

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Agricultural Sciences 2022年第2期13卷 268-281页

作者： Lucas Ghedin Ghizzi Matheus Rebouças Pupo Celso Heinzen Jr. Tiago Antônio Del valle Francisco Palma Rennó Luiz Felipe Ferraretto Department of Animal Nutrition and Animal Production University of Sao Paulo Pirassununga Brazil Department of Animal and Dairy Sciences University of Wisconsin Madison USA Federal University of Santa Maria Santa Maria Brazil

Whole-plant soybean ensiling has limitations and challenges that affect silage fermentation patterns and reduce ruminal nutrient degradation. Perhaps either the addition of molasses at ensiling or harvesting at different phenological stages has the potential to enhance whole-plant soybean silage (WSS) ruminal degradation. This experiment was a completely randomized design with a 3 × 2 factorial arrangement of treatments evaluating the effects of molasses (0 and 40 g/kg fresh forage) and phenological stage (R5: beginning seed, R6: full seed, and R7: beginning maturity) on ruminal in situ degradation. Molasses increased effective ruminal degradability (ERD) of dry matter (DM) regardless of the phenological stage and increased ERD of crude protein at R5 and R6. The addition of molasses at later phenological stages increased ERD of neutral detergent fiber (NDF). There was a molasses × phenological stage interaction effect with greater fractions A and B of NDF at R7 and R5, respectively, but lower undegraded NDF at R5 and R6 with molasses addition. Although the benefits of adding molasses were more pronounced in R5 and R6, adding molasses to whole-plant soybean at ensiling is recommended regardless of phenological stage. Increasing the proportion of digestible nutrients at harvesting either by harvesting at later phenological stages or the addition of molasses is a powerful strategy to manage ruminal degradation of DM and nutrients in WSS.

关键词： Additive In Situ Degradation Ruminal Degradation Parameters Legume Ensiling

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Is glucose-6-phosphate dehydrogenase deficiency more prevalent in Carrion's disease endemic areas in Latin America?

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Asian Pacific Journal of Tropical Medicine 2015年第12期8卷 1045-1046页

作者： Fernando Mazulis Claudia Weilg Carlos Alva-Urcia Maria J.Pons Juana del valle Mendoza School of Medicine Research Center of the Health Sciences FacultyUniversidad Peruana de Ciencias Aplicadas(UPC)

Glucose-6-phosphate dehydrogenase(G6PD) is a cytoplasmic enzyme with an important function in cell oxidative damage *** have a predisposition towards oxidized environments due to their lack of mitochondria,giving G6 PD a major role in its stability.G6 PD deficiency(G6PDd) is the most common enzyme deficiency in humans:it affects approximately 400 million individuals *** overall G6 PDd allele frequency across malaria endemic countries is estimated to be 8%.corresponding to approximately 220 million males and 133 million ***,there are no reports on the prevalence of G6 PDd in Andean communities where bartonellosis is prevalent.

关键词： Glucose-6-phosphate dehydrogenase G6PD Bartonella

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Detection of dengue virus serotype 3 in Cajamarca,Peru:Molecular diagnosis and clinical characteristics

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Asian Pacific Journal of Tropical Medicine 2021年第10期14卷 456-462页

作者： Miguel Angel Aguilar-Luis Hugo Carrillo-Ng Sungmin Kym Wilmer Silva-Caso Eduardo Verne Luis J.del valle Jorge Bazán-Mayra Victor Zavaleta-Gavidia Daniel Cornejo-Pacherres Yordi Tarazona-Castro Ronald Aquino-Ortega Angela Cornejo-Tapia Juana del valle-Mendoza School of Medicine Research and Innovation Center of the Faculty of Health SciencesUniversidad Peruana de Ciencias AplicadasLimaPeru Laboratorio de Biologia Molecular Instituto de Investigación NutricionalLimaPeru Facultad de Medicina Alberto Hurtado Universidad Peruana Cayetano HerediaLimaPeru Division of Infectious Diseases Department of Internal MedicineChungnam National University School of MedicineDaejeonRepublic of Korea Barcelona Research Center for Multiscale Science and Engineering Departament d'Enginyeria QuímicaEEBEUniversitat Politècnica de Catalunya(UPC)BarcelonaSpain Laboratorio Regional de Cajamarca Dirección Regional de Salud de Cajamarca(DIRESA)CajamarcaPeru

Objective:To describe and molecularly characterize an outbreak of dengue virus(DENV)infection in Cajamarca,an Andean region in ***:A total of 359 serum samples from patients with acute febrile illness were assessed for the presence of DENV via RT-PCR,ELISA NS1,Ig M and Ig G in Cajamarca,Peru from January 2017 to June *** evaluation of the different diagnostic tests and their applicability was ***:Dengue virus was detected in 24.7%of samples by ***,serological analysis detected 30.3%positive cases via ELISA NS1 antigen,16.7%via ELISA Ig G and 9.7%via ELISA Ig *** of the cases corresponded to DENV-3(77.5%).The use of RT-PCR performed better in primary infections(P<0.01),while detection of ELISA Ig M performed better in secondary infections(P<0.01).The combination of NS1 and Ig M performed better than the other assays in detecting primary(92.5%)and secondary infections(96.6%).The most frequent symptoms associated with fever were headaches,myalgias,and arthralgias across all ***:We report an important outbreak of dengue infection caused by DENV-3 in Cajamarca,*** findings encourage the use of NS1 antigen and Ig M *** findings demonstrate an increasing expansion of DENV-3 in Peru and highlight the importance of molecular diagnosis and serotype characterization among the clinically defined dengue cases to strengthen the Peruvian epidemiological surveillance.

关键词： Dengue DENV Arbovirus Diagnosis Peru

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Increasing rim height depth for safe femtosecond laser-assisted capsulotomy in intumescent white cataracts with liquified cortex

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International Journal of Ophthalmology(English edition) 2022年第9期15卷 1557-1559页

作者： Beatriz Vidal-Villegas JoséAntonio Gegúndez Fernández Bárbara Burgos-Blasco David Diaz-valle Ricardo Cuiña-Sardiña Rosalía Méndez-Fernández Pedro Arriola-Villalobos Mayte Ariño-Gutiérrez Jose Manuel Benítez-del-Castillo Ophthalmology Service Hospital Clínico San CarlosProfesor Martin Lagos 2Madrid 28040Spain

Dear Editor,Continuous curvilinear capsulorhexis is an essential part of modern cataract surgery,as it safely enables phacoemulsification procedures and permits a stable intraocular lens(IOL)bag *** white cataracts wi... 详细信息

关键词： white laser femtosecond

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Role of micronutrients in staging of nonalcoholic fatty liver disease:A retrospective cross-sectional study

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World Journal of Gastrointestinal Surgery 2020年第6期12卷 269-276页

作者： Franciele Sabadin Bertol Bruna Araujo Brunno Brochado Jorge Natalino Rinaldi Luiz Alberto De Carli Cristiane valle Tovo Graduate Program of Medicine HepatologyUniversidade Federal de Ciências da Saúde de Porto AlegrePorto AlegreRS 90430080Brazil

BACKGROUND Nonalcoholic fatty liver disease(NAFLD) presents high incidence throughout the world and has been progressively increasing in prevalence. This disease has a heterogeneous natural history, including simple steatosis, nonalcoholic steatohepatitis(NASH), and cirrhosis. The factors that determine its evolution to more severe forms of the disease are still poorly understood, and micronutrients with antioxidant potential may be involved in the pathophysiology of the *** To evaluate the relationship between serum levels of micronutrients and the severity of *** A retrospective, observational and cross-sectional study was conducted. This study included all patients undergoing bariatric surgery who experienced liver biopsy during the procedure, and had serum levels of micronutrients(vitamin D,vitamin B12, zinc, iron, and magnesium), which was assessed in a preoperative evaluation conducted at a reference center in southern *** A total of 614 patients were analyzed, of which 93% had steatosis, 70.7% had NASH, and 49.3% had some degree of fibrosis. Serum levels of vitamin D were negatively correlated with the severity of steatosis and NASH, and serum levels of vitamin B12 were positively correlated with the severity of steatosis and fibrosis. The other micronutrients showed no association with NAFLD *** Serum levels of vitamin D are inversely related to the severity of steatosis and NASH, and serum levels of vitamin B12 are higher in more advanced stages of simple steatosis and liver fibrosis. Serum levels of zinc, iron, and magnesium were not associated with NAFLD severity.

关键词： Nonalcoholic fatty liver disease Nonalcoholic steatohepatitis Liver cirrhosis Micronutrients Vitamin D Vitamin B12 Zinc Iron Magnesium

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Chromosome Scale Genome Assembly andTranscriptome Profiling of Nannochloropsisgaditana in Nitrogen Depletion

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Molecular Plant 2014年第2期7卷 323-335页

作者： Elisa Corteggiani Carpinelli Andrea Telatin Nicola Vitulo Claudio Forcato Michela D’Angelo Riccardo Schiavon Alessandro Vezzi Giorgio Mario Giacometti Tomas Morosinotto Giorgio valle CRIBI Biotechnology Centre University of Padua Padua 35121 Italy Department of Biology University of Padua Padua 35121 Italy

Nannochloropsis is rapidly emerging as a model organism for the study of biofuel production in ***, we report a high-quality genomic assembly of Nannochloropsis gaditana, consisting of large contigs, up to 500 kbplong, and scaffolds that in most cases span the entire length of the chromosomes. We identified 10646 complete genesand characterized possible alternative transcripts. The annotation of the predicted genes and the analysis of cellular pro-cesses revealed traits relevant for the genetic improvement of this organism such as genes involved in DNA recombina-tion, RNA silencing, and cell wall synthesis. We also analyzed the modification of the transcriptional profile in nitrogendeficiencyma condition known to stimulate lipid accumulation. While the content of lipids increased, we did not detectmajor changes in expression of the genes involved in their biosynthesis. At the same time, we observed a very signifi-cant down-regulation of mitochondrial gene expression, suggesting that part of the AcetyI-CoA and NAD（P）H, normallyoxidized through the mitochondrial respiration, would be made available for fatty acids synthesis, increasing the fluxthrough the lipid biosynthetic pathway. Finally, we released an information resource of the genomic data of IV. gaditana,available online at ***.

关键词： Nannochloropsis lipids biofuel genome transcriptome de novo assembly SOLiD nitrogen deprivation chromosomes telomeres

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Antibacterial activity of Myrciaria dubia(Camu camu) against Streptococcus mutans and Streptococcus sanguinis

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 740-744页

作者： Rosella Camere-Colarossi Gabriela Ulloa-Urizar Dyanne Medina-Flores Stefany Caballero-García Frank Mayta-Tovalino Juana del valle-Mendoza School of Dentistry Faculty of Health SciencesPeruvian University of Applied Sciences-UPC Research and Innovation Center of the Faculty of Health Sciences Peruvian University of Applied Sciences-UPC Molecular Biology Laboratory Nutrition Research Institute

Objective:To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia(Camu camu)(***) methanol extract,against Streptococcus mutans(ATCC 25175)(***) and Streptococcus sanguinis(ATCC 10556)(***).Methods:Two methanol extracts of *** were prepared in vitro,from the seeds and *** independent tests were prepared for each type of extract,using 0.12% chlorhexidine solution as positive *** diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at ***,the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was ***:A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of(21.36 ± 6.35) mm and(19.21 ± 5.18) mm against *** and ***,*** methanol extract of the pulp had an effect of(16.20 ± 2.08)mm and(19.34 ± 2.90) mm,*** minimum inhibitory concentration of the pulp extract was 62.5 mg/mL for both strains,whereas for the seed antibacterial activity was observed even at low *** CC50 of the seeds extract was at a higher concentration than 800 mg/mL and 524.37 mg/mL for the pulp ***:The experimental findings demonstrated the antibacterial effect of the methanol extract of *** against *** and *** extracts were not cytotoxic at high concentrations.

关键词： Antibacterial effect Medicinal plants Myrciaria dubia Cytotoxicity

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