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Novel technique for anastomotic salvage using transanal minimally invasive surgery:A case report

World Journal of Gastrointestinal Surgery 2019年第5期11卷 271-278页

作者： Oscar A Olavarria robert L kress Shinil k Shah Amit k Agarwal Center for Surgical Trials and Evidence-Based Practice McGovern Medical School at the University of Texas Health Science Center at HoustonHoustonTX 77033United States Department of Surgery McGovern Medical School at the University of Texas Health Science Center HoustonHoustonTX 77033United States

BACkGROUND Anastomotic leak(AL) after low anterior resection(LAR) can be a highly morbid *** incidence of AL ranges from 5% to 20% depending on patient characteristics and the distance of the anastomosis from the anal *** anastomoses and leaks pose technical challenges for endoscopic *** aim of this report was to describe the use of a commercially available laparoscopic energy device through a transanal minimally invasive surgery(TAMIS) port for the management of a symptomatic leak not requiring relaparotomy(grade B) after a LAR with diverting loop *** SUMMARY A TAMIS GelPOINT Path port was inserted into the anus to access the distal *** was achieved with AirSeal insufflation and a 30 degree laparoscope was introduced through a trocar.A LigaSure TM Retractable L-Hook device was then used to perform a septotomy of the chronic sinus tract identified posterior to the *** procedure was then repeated twice in three weeks intervals with ultimate resolution of the chronic leak *** months after serial TAMIS septotomies,barium enema demonstrated a patent anastomosis with no evidence of persistent leak or *** patient subsequently underwent ileostomy reversal and has had no significant postoperative *** TAMIS septotomy with the LigaSure TM Retractable L-Hook is a feasible andeffective,minimally invasive salvage technique for the treatment of grade B *** studies are needed to assess the generalizability and long-term results of this technique.

关键词： Transanal minimally invasive surgery Ligasure hook Anastomotic leak Low anterior resection Septotomy Case report

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THE SCATTERING OF GENERAL SH PLANE WAVE BY INTERFACE CRACk BETWEEN TWO DISSIMILAR VISCOELASTIC BODIES

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Acta Mechanica Sinica 1999年第3期15卷 245-254页

作者：魏培君张双寅吴永礼 robert k.Y.Li Laboratory for Nonlinear Mechanics of Continuous Media Institute of MechanicsChinese Academy of ScienceBeijing 100080China Department of Physics and Materials Science City University of Hong KongChina

The scattering of general SH plane wave by an interface crack between two dissimilar viscoelastic bodies is studied and the dynamic stress intensity factor at the crack-tip is computed. The scattering problem can be decomposed into two problems: one is the reflection and refraction problem of general SH plane waves at perfect interface (with no crack); another is the scattering problem due to the existence of crack. For the first problem, the viscoelastic wave equation, displacement and stress continuity conditions across the interface are used to obtain the shear stress distribution at the interface. For the second problem, the integral transformation method is used to reduce the scattering problem into dual integral equations. Then, the dual integral equations are transformed into the Cauchy singular integral equation of first kind by introduction of the crack dislocation density function. Finally, the singular integral equation is solved by kurtz's piecewise continuous function method. As a consequence, the crack opening displacement and dynamic stress intensity factor are obtained. At the end of the paper, a numerical example is given. The effects of incident angle, incident frequency and viscoelastic material parameters are analyzed. It is found that there is a frequency region for viscoelastic material within which the viscoelastic effects cannot be ignored.

关键词： viscoelasticity interface crack general plane wave integral transformation singular integral equations

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Novel fiber-optical interferometer with miniaturized probe for in-hole measurements

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Optoelectronics Letters 2008年第2期4卷 140-142页

作者： robert Schmitt Tilo Pfeifer Frank Depiereux Niels knig Fraunhofer Institute for Production Technology (IPT) Steinbachstrasse 17 52074 Aachen Germany

Today, micro-system technology and the development of new MEMS (Micro-Electro-Mechanical Systems) are emerging rapidly. In order for this development to become a success in the long run, measurement systems have to ensure product quality. Most often, MEMS have to be tested by means of functionality or destructive tests. One reason for this is that there are no suitable systems or sensing probes available which can be used for the measurement of quasi inaccessible features like small holes or cavities. We present a measurement system that could be used for these kinds of measurements. The system combines a fiber optical, miniaturized sensing probe with low-coherence interferometry, so that absolute distance measurements with nanometer accuracy are possible.

关键词：纤维光干扰术光检测技术测量方法

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Cold-temperature deformation of nano-sized tungsten and niobium as revealed by in-situ nano-mechanical experiments

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Science China(Technological Sciences) 2014年第4期57卷 652-662页

作者： LEE Seok-Woo CHENG YinTong ryu Ill GREER Julia R. Division of Engineering and Applied Science California Institute of Technology School of Engineering Brown University

We constructed and developed an in-situ cryogenic nanomechanical system to study small-scale mechanical behavior of materials at low temperatures. Uniaxial compression of two body-centered-cubic （bcc） metals, Nb and W, with diameters between 400 and 1300 rim, was studied at room temperature and at 165 k. Experiments were conducted inside of a Scanning Electron Microscope （SEM） equipped with a nanomechanical module, with simultaneous cooling of sample and diamond tip. Stress-strain data at 165 k exhibited higher yield strengths and more extensive strain bursts on average, as compared to those at 298 k. We discuss these differences in the framework of nano-sized plasticity and intrinsic lattice resistance. Dislocation dynamics simulations with surface-controlled dislocation multiplication were used to gain insight into size and temperature effects on deformation of nano-sized bcc metals.

关键词： dislocation plasticity metal nanopillar cryogenics

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Over-processed meat and bone meal and phytase effects on broilers challenged with subclinical necrotic enteritis:Part ***,intestinal lesions and pH,bacterial counts and apparent ileal digestibility

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Animal Nutrition 2020年第4期6卷 313-324页

作者： Holy k.Zanu Sarbast k.kheravii Natalie k.Morgan Michael R.Bedford robert A.Swick School of Environmental and Rural Science University of New EnglandArmidaleNEW 2351Australia AB Vista MarlboroughWiltshire SN84ANUnited Kingdow

This feeding study investigated the hypothesis that over-processing of meat and bone meal(MBM)would impair the performance,gut health and ileal digestibility of nutrients in birds challenged with necrotic enteritis(NE).The effect of phytase(500 vs.5,000 FTU/kg)was also examined using manufacturers recommended matrix values for 500 FTU for both *** 308 male broilers(n=768)were assigned to 8 diets,with 6 replicate pens per diet and 16 birds per replicate pen using a randomized design with a factorial arrangement of *** were NE challenge(no or yes),MBM(as received or overprocessed),and phytase level(500 or 5,000 FTU/kg).Half of the birds were challenged with 5,000 oocysts of field strains of Eimeria acervulina and Eimeria brunetti,and 2,500 oocysts of Eimeria maxima on d 9 and 10^8 CFU/mL of Clostridium perfringens strain EHE-NE18 on d 14 and 15 ***×MBM interactions were detected for weight gain(WG),feed conversion ratio(FCR)and feed intake(FI)at d 14,21 and 28,showing that challenged birds fed over-processed MBM had decreased WG(P0.05)at d *** fed low phytase had increased livability(P<0.05)at d *** challenge increased the prevalence and severity of NE induced lesions in the jejunum(P<0.05)and ileum(P<0.05).The birds fed over-processed MBM had decreased pH in the jejunum(P<0.05)and ileum(P<0.05)at d *** phytase increased apparent ileal digestibility(AID)of Ca(P<0.05)and P(P<0.05),and over-processed MBM increased AID of carbon(C;P<0.05)and Ca(P<0.05)at d *** challenge increased the caecal counts of Lactobacillus spp.(P<0.05)and ***(P<0.05)at d *** results indicated that supplementation of diets with high phytase reduces the negative impact on performance from over-processed MBM during NE as a result of increased nutrient digestibility.

关键词： Broilers Indigestible proteins Meat and bone meal Necrotic enteritis Phytase

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Cleavage of the Carboxyl-Terminus of LEACS2, a Tomato 1-Aminocyclopropane-l-Carboxylic Acid Synthase Isomer, by a 64-kDa Tomato Metalloprotease Produces a Truncated but Active Enzyme

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Journal of Integrative Plant Biology 2005年第11期47卷 1352-1363页

作者： Jian-Feng LI robert QI Liang-Hu QU Autar k Mattoo Ning LI Department of Biology The Hong Kong University of Science and Technology Hong Kong SAR China key Laboratory of Gene Engineering of the Ministry of Education Zhongshan University Guangzhou 510275 China Department of Biochemistry The Hong kong University of Science and Technology Hong Kong SAR China Sustainable Agricultural Systems Laboratory United States Department of Agriculture-Agriculture Research Service Beltsville Maryland 20705-2350 USA

1-Aminocyclopropane-1-carboxylic acid （ACC） synthase （ACS） is the principal enzyme in phytohormone ethylene biosynthesis. Previous studies have shown that the hypervariable C-terminus of ACS is proteolytically processed in vivo. However, the protease responsible for this has not yet been identified. In the present study, we investigated the processing of the 55-kDa full-length tomato ACS （LeACS2） into 52-, 50- and 49-kDa truncated isoforms in ripening tomato （Lycopersicon esculentum Mill. cv. Cooperation 903） fruit using the sodium dodecyl sulfate-boiling method. Meanwhile, an LeACS2-processing protease was purified via multi-step column chromatography from tomato fruit. Subsequent biochemical analysis of the 64-kDa purified protease revealed that it is a metalloprotease active at multiple cleavage sites within the hypervariable C-terminus of LeACS2. N-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight analysis indicated that the LeACS2-processing metalloprotease cleaves at the C-terminal sites Lys^438, Glu^447, Lys^448, Asn^456, Ser^460, Ser^462, Lys^463, and Leu^474, but does not cleave the N- terminus of LeACS2. Four C-terminus-deleted （26-50 amino acids） LeACS2 fusion proteins were overproduced and subjected to proteolysis by this metalloprotease to identify the multiple cleavage sites located on the N-terminal side of the phosphorylation site Ser^460. The results indisputably confirmed the presence of cleavage sites within the region between the α-helix domain （H14） and Ser^460 for this metalloprotease. Furthermore, the resulting C-terminally truncated LeACS2 isoforms were active enzymatically. Because this protease could produce LeACS2 isoforms in vitro similar to those detected in vivo, it is proposed that this metalloprotease may be involved in the proteolysis of LeACS2 in vivo.

关键词： 1-aminocyclopropane-1-carboxylic acid synthase cleavage sites metalloprotease proteolytic processing.

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Effects of L-arginine and L-citrulline supplementation in reduced protein diets for broilers under normal and cyclic warm temperature

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Animal Nutrition 2021年第4期7卷 927-938页

作者： Hiep Thi Dao Nishchal k.Sharma Emma J.Bradbury robert A.Swick School of Environmental and Rural Science Faculty of ScienceAgricultureBusiness and LawUniversity of New EnglandArmidaleAustralia Faculty of Animal Science Vietnam National University of AgricultureTrau Quy TownGia Lam DistrictHanoiViet Nam Ridley AgriProducts MelbourneVictoriaAustralia

Heat stress causes significant economic losses in the broiler *** supplementation of arginine(Arg)and citrulline(Cit)might increase the performance of broilers raised under warm temperature due to vasodilation *** study investigated the effects of L-Arg or L-Cit supplementation in broilers fed a reduced protein wheat-based diet deficient in Arg under thermoneutral(NT)and cyclic warm temperature(WT).Ross 308 cockerels(n=720)were randomly allocated to 4 dietary treatments with 12 replicates of 15 birds per pen from d 7 to *** 4 treatments were:normal protein(NP),i.e.,22.3%and 20.9%crude protein in grower and finisher,respectively;reduced protein(RP),i.e.,2.5%lower protein and deficient in Arg;and RP supplemented with 0.28%Arg(RP-Arg)or 0.28%Cit(RP-Cit).A factorial arrangement of treatments was applied during the finisher phase(21 to 35 d).Factors were:diet(4 diets above);and temperature,NT(24℃)or cyclic WT(33±1℃for 6 h per day)with 6 replicate pens per *** 7 to 35 d and 21 to 35 d,the birds fed the RP diet had lower body weight gain(BWG)and higher FCR compared to the NP diet(Pke(FI),lower BWG(P0.05)compared to birds exposed to *** by temperature interactions were not observed for performance parameters during the period of WT(P>0.05).On d 35,the RP-fed birds had a lower yield of thigh and drumstick,higher fat pad,lower femur ash,and breaking strength but similar serum uric acid level and higher nitrogen digestibility on d 21 compared to those offered NP(P<0.05).Supplementation of Arg or Cit to RP resulted in increased femur ash on d 35(P<0.05).Thus,feeding the NP diets is necessary to maintain growth performance in broilers regardless of the temperature conditions.

关键词： Arginine Citrulline Meat chickens Low protein Heat stress

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Adhesive ginsenoside compound k patches for cartilage tissue regeneration

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Regenerative Biomaterials 2023年第1期10卷 1442-1450页

作者： Jun-Ho Yang Hyun Ho Shin Donghyeon kim Ji Hyun ryu Eun-Jung Jin Department of Biological Sciences College of Health SciencesWonkwang UniversityIksanJeonbuk 54538South Korea Department of Chemical Engineering Wonkwang UniversityIksanJeonbuk 54538South Korea Department of Carbon Convergence Engineering Wonkwang UniversityIksanJeonbuk 54538South Korea Integrated Omics Institute Wonkwang UniversityIksanJeonbuk 54538South Korea

Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery *** instance,compound k(Ck)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune ***,its effects on cartilage degradation and tissue regeneration remain ***,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for Ck *** this study,Ck-loaded adhesive patches were reported to seal cartilage defects and deliver Ck to defect sites,preventing cartilage degradation and accelerating cartilage tissue *** patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage *** addition,there were no significant differences in the adhesive polymeric networks before and after Ck ***-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing Ck in cartilage ***,the NFkB signaling pathway of released Ck from the adhesive patches in the treatment of osteoarthritis is ***,the Ck-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.

关键词： ginsenoside compound k adhesive patch drug delivery hydrocaffeic acid-conjugated chitosan osteoarthritis

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Response of laying hens to L-arginine,L-citrulline and guanidinoacetic acid supplementation in reduced protein diet

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Animal Nutrition 2021年第2期7卷 460-471页

作者： Hiep Thi Dao Nishchal k.Sharma Emma J.Bradbury robert A.Swick School of Environmental and Rural Science Faculty of ScienceAgricultureBusiness and LawUniversity of New England2351New South WalesAustralia Faculty of Animal Science Vietnam National University of AgricultureTrau Quy TownGia Lam DistrictHanoiViet Nam Ridley AgriProducts Level 4565 Bourke StreetMelbourneVICAustralia

A study was conducted with Hy-Line Brown laying hens to examine the effects of reduced protein diet,deficiency of arginine(Arg),and addition of crystalline Arg,citrulline(Cit)and guanidinoacetic acid(GAA)as substitutes for *** performance,egg quality,serum uric acid,liver and reproductive organ weights,and energy and protein digestibility were measured using a completely randomized design with 5 *** were a standard diet(17%protein diet;SP),a reduced diet(13%protein diet deficient in Arg;RP)and RP with added Arg(0.35%,RP-Arg),GAA(0.46%equivalent to 0.35%Arg,RP-GAA)or Cit(0.35%,RP-Cit)to the level of *** was hypothesized that performance would decrease with Arg deficient RP diet and the addition of GAA or Cit in RP would allow birds to perform similar or greater than Arg-added RP *** experiment was conducted from 20 to 39 wk of age but the treatment effect was seen only after 29 wk of *** birds offered RP had reduced egg and albumin weights(Pk color score(Pke and excretion(Pke them equivalent to SP,feed intake(FI)and egg production were not different than those of RP(P>0.05).The birds offered RP-GAA decreased FI and egg production(P0.05).However,birds offered the RP-Cit diet tended to have higher Haugh unit(P=0.095)and lower shell breaking strength(P=0.088)compared to all other treatments while those offered RP-GAA had higher energy digestibility(P<0.05)than all other groups but *** limited performance response of hens fed RP with added Arg,GAA,or Cit may be due to deficiency of some other nutrients in RP such as phenylalanine,potassium or non-essential amino acids and other components of soybean meal in the diet.

关键词： Arginine Citrulline Guanidinoacetic acid Laying hen Reduced protein

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Utilizing Multilayer Design of Organic-Inorganic Hybrids to Enhance Wearable Strain Sensor in Humid Environment

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Chinese Journal of Polymer Science 2023年第7期41卷 1037-1050页

作者： Chen-Chen Lu Wei-Chen Gao Peng Li Wei Wu robert k.Y.Li Hui Zhao Guangxi key Laboratory of Clean Pulp&Papermaking and Pollution Control School of Light Industry and Food EngineeringGuangxi UniversityNanning530004China Guangxi key Laboratory of Calcium Carbonate Resources Comprehensive Utilization College of Materials and Chemical EngineeringHezhou UniversityHezhou542899China Department of Materials Science and Engineering City University of Hong KongTat Chee AvenueKowloonHong Kong999077China

Flexible strain wearable sensors have attracted considerable attention due to their advantages of low cost, lightweight, high sensitivity and good flexibility. However, the strain sensors are easy to be damaged in an extreme humidity environment or by the wearer’s sweat in the process of use, resulting in detection disorder or even a short circuit. Furthermore, preparation of sensors with stable properties under extreme environments is one of the most important research directions. To fill this gap, a flexible sensor was prepared by using polyurethane and carbon nanotubes, then modified by polydopamine and 1H,1H,2H,2H-perfluorodecane-mercaptan. A typical tunnel model was used to explain the working mechanism of the sensor, the sensitivity of the sensor is also explained and evaluated by the tunneling theory. The results show that the sensor has good sensitivity (the sensor has a stable sensing signal output under a strain range from 2% to 300%) and stability over 8500 cycles. At the same time, the sensor has good superhydrophobicity, the water contact angle reaches 152°, and it is still stable in a humid environment. Moreover, this sensor shows excellent performance in monitoring human joint motion (such as finger, elbow, wrist and knee) and physiological signals (such as speaking and drinking). This work provides an effective design method for the sensor which can be applied in a high humidity environment.

关键词： Wearable strain sensor Polyurethane Carbon nanotubes Superhydrophobicity

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