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Carbon dioxide enrichment affected flower numbers transiently and increased successful post-pollination development stably but without altering final acorn production in mature pedunculate oak (Quercus robur L.)

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Journal of Forestry Research 2024年第4期35卷 1-12页

作者： Ryan McClory richard H.Ellis Martin Lukac Jo Clark Carolina Mayoral Kris M.Hart Andrew R.G.Plackett A.Rob MacKenzie School of Agriculture Policy and DevelopmentUniversity of ReadingAgricultureEarley GateWhiteknights RoadPO Box 237Reading RG66EUUK Faculty of Forestry and Wood Sciences Czech University of Life Sciences PraguePrague 165000Czechia Future Trees Trust Harwell Innovation CentreBuilding 173Curie AvenueHarwellOxford OX110QGUK Birmingham Institute of Forest Research University of BirminghamEdgbastonBirmingham B152TTUK School of Biosciences University of BirminghamEdgbastonBirmingham B152TTUK School of Geography Earth and Environmental SciencesUniversity of BirminghamEdgbastonBirmingham B152TTUK

Acorn production in oak(Quercus spp.)shows considerable inter-annual variation,known as masting,which provides a natural defence against seed predators but a highly-variable supply of acorns for uses such as in commercial tree planting each *** emissions of greenhouse gases have been very widely reported to influence plant growth and seed or fruit size and quantity via the‘fertilisation effect’that leads to enhanced *** examine if acorn production in mature woodland communities will be affected by further increase in CO_(2),the contents of litter traps from a Free Air Carbon Enrichment(FACE)experiment in deciduous woodland in central England were analysed for numbers of flowers and acorns of pedunculate oak(Quercus robur L.)at different stages of development and their predation levels under ambient and elevated CO_(2) ***-annual variation in acorn numbers was considerable and cyclical between 2015 and 2021,with the greatest numbers of mature acorns in 2015,2017 and 2020 but almost none in *** numbers of flowers,enlarged cups,immature acorns,empty acorn cups,and galls in the litter traps also varied amongst years;comparatively high numbers of enlarged cups were recorded in 2018,suggesting *** at this site is a fruit maturation masting species(i.e.,the extent of abortion of pollinated flowers during acorn development affects mature acorn numbers greatly).Raising the atmospheric CO_(2) concentration by 150μL L^(−1),from early 2017,increased the numbers of immature acorns,and all acorn evidence(empty cups+immature acorns+mature acorns)detected in the litter traps compared to ambient controls by 2021,but did not consistently affect the numbers of flowers,enlarged cups,empty cups,or mature *** number of flowers in the elevated CO_(2) plots’litter traps was greater in 2018 than 2017,one year after CO_(2) enrichment began,whereas numbers declined in ambient *** with CO_(2) also increased the number o

关键词： Quercus robur L. Acorns Masting Pedunculate oak Carbon dioxide

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Is there hybridization between 2 species of the same genus in sympatry?——The genetic relationships between Anoplophora glabripennis,Anoplophora chinensis,and putative hybrids

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Insect Science 2024年第2期31卷 633-645页

作者： Haiwen Qin Huachao Xu Arnaud Capron Ilga Porth Mingming Cui Melody A.Keena Xiaofang Deng Juan Shi richard C.Hamelin Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia Department of ForestBejing Forestry UniversityBejingChina Department of Forest and Conservation Sciences The University of British ColumbiaVancouverCanada College of Forestry and Biotechnology Zhejiang A&F UniversityHangzhouChina Department of Wood and Forest Sciences Laval UniversityQuebecCanada 5 Department of AgricultureNorthern Research StationUSDA Forest ServiceHamdenConnecticutUSA Changchun Landscape Plant Conservation Station Bureau of Forestry and Landscaping of ChangchunChangchunChina

Anoplophora glabripennis(Asian longhorn beetle,ALB)and Anoplophora chinensis(Citrus longhorn beetle,CLB)are native forest pests in China;they have become important international quarantine *** are found using the same Salix aureopendula host tree of Cixi,Zhejiang province,*** this host tree,we collected additional beetles that appeared to be morphologically intermediate between ALB and *** using a stereoscope,we observed that there were several bumps on the base of the elytra,which was inconsistent with ALB,which typically has a smooth elytral base,but was more like CLB,which has numerous short tubercles on the elytral *** their sympatry and intermediate morphology,we hypothesized that these may represent ALB×CLB *** studied the genomic profiles for 46 samples(ALB,CLB,and putative hybrids)using genotyping-by-sequencing(GBS)providing a reduced representation of the entire *** principal component analyses on the 163 GBS-derived single nucleotide polymorphism data,we found putative hybrids tightly clustered with ALB,but genetically distinct from the CLB ***,our initial hybrid hypothesis was not supported by genomic ***,while mating experiments between adult ALB and CLB were successful in 4 separate years(2017,2018,2020,and 2021),and oviposition behavior was observed,no progeny was *** employed population genomic analysis and biological hybridization experiments,we conclude that the putative hybrids represent newly discovered morphological variants within *** approach further confirmed the advantage of genome-wide information for Anoplophora species assignment in certain ambiguous classification cases.

关键词： Anoplophora chinensis Anoplophora glabripenni sg enotyping-bysequencing invasive pest mating putative hybrid

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西北太平洋鲐的资源养护和管理研究

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中国水产科学 2024年第4期31卷 439-453页

作者：蔡锴周雨霏麻秋云 KINDONG richard 上海海洋大学海洋科学学院浙江大学海洋学院国家远洋渔业工程技术研究中心大洋渔业资源可持续开发教育部重点实验室

鲐(Scomberjaponicus)是西北太平洋海域重要的渔业种类,该渔业涉及多个国家的多种作业方式,渔业形势较复杂,但目前西北太平洋海域尚无基于资源评估的、完善的鲐养护管理措施。本研究使用传统渔业管理方法(资源预测)和较新颖的方法[数据... 详细信息

鲐(Scomberjaponicus)是西北太平洋海域重要的渔业种类,该渔业涉及多个国家的多种作业方式,渔业形势较复杂,但目前西北太平洋海域尚无基于资源评估的、完善的鲐养护管理措施。本研究使用传统渔业管理方法(资源预测)和较新颖的方法[数据有限的渔业管理规程评价(managementstrategyevaluation,MSE)]对鲐的渔业管理进行了研究。在资源预测方法中,通过JABBA和JABBA-Select两个资源评估模型对种群未来的资源动态进行模拟,探讨不同捕捞控制规则(harvest control rules, HCR)下种群的变化。本研究设置了4个HCR,分别以2020年渔获量的0.8～1.4倍,最大可持续产量MSY、B×FMSY和SB×HMSY (分别用于JABBA和JABBA-Select)、历史最高渔获量为总可捕量(totalallowablecatch,TAC),预测2023—2032年间的鲐种群变化。结果显示基于亲体资源量进行研究的JABBA-Select模型,其预测结果相比于JABBA模型略悲观,但都是基于生物学参考点设定的HCR养护和管理效果最好(TAC分别为50～80万t和50～66万t)。MSE则根据设定筛选出12种养护管理效果较好的策略,其中DAAC(消耗率修正后的平均捕获量)与SBT2 (基于资源量和渔获量水平的总可捕量递增矫正法)养护管理效果最好。SBT2求得的TAC (520387 t),与近些年产量及上述HCR的结果较为接近。此外,敏感性分析表明,可捕系数,自然死亡系数和渔获量相关的参数(平均渔获量、渔获量、渔获量参考点)对于养护管理效果的影响最为明显,需要额外关注两者的准确性和精确性。现阶段为确保西北太平洋鲐资源的养护和最佳利用,建议根据B×FMSY或SB×HMSY设定年可捕量,同时基于资源量和渔获量水平进行调整,综合制定管理措施。

关键词：渔业管理有限数据方法种群预测总可捕量

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Biofabrication of nanocomposite-based scaffolds containing human bone extracellularmatrix for the differentiation of skeletal stem and progenitor cells

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Bio-Design and Manufacturing 2024年第2期7卷 121-136页

作者： Yang-Hee Kim Janos M.Kanczler Stuart Lanham Andrew Rawlings Marta Roldo Gianluca Tozzi Jonathan I.Dawson Gianluca Cidonio richard O.C.Oreffo Faculty of Medicine Bone and Joint Research GroupCentre for Human DevelopmentStem Cells and RegenerationInstitute of Developmental SciencesUniversity of SouthamptonSouthampton SO166YDUK School of Pharmacy and Biomedical Science University of PortsmouthPortsmouth PO12DTUK School of Engineering Faculty of Engineering and ScienceUniversity of GreenwichGreenwich ME44TBUK Center for Life Nano-and Neuro-Science(CLN2S) Italian Institute of Technology00161 RomeItaly

Autograft or metal implants are routinely used in skeletal ***,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering *** use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-dimensional(3D)bioprintingof skeletal tissueis anattractivestrategyfor ***,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic *** this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based *** inclusion of the nanoﬁller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon *** composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)*** inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven *** study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

关键词： Extracellular matrix Nanoclay Bone 3D bioprinting

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The Effect of Retinoic Acid on Neutrophil Innate Immune Interactions With Cutaneous Bacterial Pathogens

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Infectious Microbes & Diseases 2024年第2期6卷 65-73页

作者： Alexandra Stream Ross Corriden Simon Döhrmann richard L.Gallo Victor Nizet Ericka L.Anderson Division of Host-Microbe Systems and Therapeutics Department of PediatricsUniversity of California San Diego School of MedicineLa JollaCaliforniaUSA Department of Dermatology University of California San Diego School of MedicineLa JollaCaliforniaUSA Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San DiegoLa JollaCaliforniaUSA

Vitamin A and its biologically active derivative,retinoic acid(RA),are important for many immune ***,in particular,is essential for the development of immune cells,including neutrophils,which serve as a front-line defense against *** vitamin A deficiency has been linked to higher susceptibility to infections,the precise role of vitamin A/RA in host-pathogen interactions remains poorly ***,we provided evidence that RA boosts neutrophil killing of methicillin-resistant Staphylococcus aureus(MRSA).RA treatment stimulated primary human neutrophils to produce reactive oxygen species,neutrophil extracellular traps and the antimicrobial peptide cathelicidin(LL-37).Because RA treatment was insufficient to reduce MRSA burden in an in vivo murine model of skin infection,we expanded our analysis to other infectious *** did not affect the growth of a number of common bacterial pathogens,including MRSA,Escherichia coli K1 and Pseudomonas aeruginosa;however,RA directly inhibited the growth of group A Streptococcus(GAS).This antimicrobial effect,likely in combination with RA-mediated neutrophil boosting,resulted in substantial GAS killing in neutrophil killing assays conducted in the presence of ***,in a murine model of GAS skin infection,topical RA treatment showed therapeutic potential by reducing both skin lesion size and bacterial *** findings suggest that RA may hold promise as a therapeutic agent against GAS and perhaps other clinically significant human pathogens.

关键词： retinoic acid neutrophil bacterial infection Staphylococcus aureus group A Streptococcus neutrophil extracellular traps antimicrobial peptide cathelicidin

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In Vivo Improvements in Facial Appearance and in Vitro Changes in Gene Expression Using a Topical Formulation Designed to Repair Environmentally Induced DNA Damage

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第2期14卷 141-173页

作者： Amaryllis Aganahi richard Parker Yohei Tanaka RATIONALE Kyneton Australia Clinica Tanaka Plastic Reconstructive Surgery and Anti-aging Center Matsumoto Japan

Background: While sunscreen has been accepted as a mainline defence against photodamage from ultraviolet, visible light and near-infrared radiation, there appears to be a lack of research into photorepair. The concept of protecting the skin during the day and repairing cellular damage at night is intuitive, yet specific strategies revolving around combinations of proven reparative active ingredients remain unelucidated. Purpose: To investigate the efficacy of a solar repair Formulation following ultraviolet and environmental exposure in order to improve overall skin health and appearance through three hypotheses: The Formulation increases expression of DNA repair mechanisms markers;The Formulation enhances overall skin appearance through reducing signs of inflammation, elevating hydration, reinforcing skin firmness and amplifying radiance;In-Vivo efficacy test results are aligned with measured gene expression changes. Methods: The Formulation (#6NIC1.V1.1-1) was tested for: In-vitro LDH cytotoxicity activity, In-vitro qPCR gene expression with and without ultraviolet exposure on a reconstructed 3-dimensional skin model, and In-Vivo efficacy study on a panel of 22 participants objectively and subjectively. Results: Skin radiance, firmness, hydration, redness, and inflammation are significantly improved after In-Vivo skin exposure to the Formulation and environmental challenges such as ultraviolet radiation. These outcomes were confirmed by in-vitro genetic testing on a reconstructed human skin model. Conclusion: The studies allowed us to identify and group results in four main skin functions that were significantly enhanced following the application of the Formulation: firmness, hydration, radiance and soothing.

关键词： Photoprotection Photorepair DNA Repair Anti-Photoaging Gene Expression Antioxidant Rejuvenation

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Behavioural Bioassays and Identification of Cashew Leaf and Stem Volatiles Mediating Attraction to the Stem Girdler, Analeptes trifasciata (Coleoptera: Cerambycidae)

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Advances in Entomology 2024年第3期12卷 170-182页

作者： Idongesit Umanah Mokwunye Olufemi richard Pitan Entomology Section Cocoa Research Institute of Nigeria Ibadan Nigeria Department of Crop Protection Federal University of Agriculture Abeokuta Abeokuta Nigeria

The cashew stem girdler, Analeptes trifasciata Fabricius (Coleoptera: Cerambycidae), damages cashew by its girdling activities in the stem thereby causing huge economic losses. The stem girdler is managed through cultural practice of burning girdled stems and beetles, though this has drawbacks. The objective of this study was to explore the cues mediating attraction to the cashew host plant;hence the role of olfaction in host plant location by A. trifasciata underlying the semio-chemical option for controlling this insect pest. A diffusional Y-tube olfactometer was used to study the behavioural response of A. trifasciata, to freshly cut cashew stem and leaves odour sources. Methanol-extract of these plant tissues was subjected to the coupled gas chromatography-mass spectrometry (GC-MS) analysis. Y-tube olfactometric assays demonstrated that both sexes oriented towards and spent significantly more time in stem odour arm compared to the leaf odour arm in both male (male: t = 2.228, d.f = 11, P = 0.040) and female (t = 2.341, d.f = 11, P = 0.040). A combination of fatty acids, amino acids and carbohydrates were detected in cashew stems. Some of these fatty acids are attractants to other insect pests. It is suspected that these fatty acid blends may possibly be responsible for facilitating host plant location by both sexes. In conclusion, both sexes were independently and strongly attracted to the stem volatiles;this study opens the possibility of utilizing cashew stem volatiles as surveillance and control tools.

关键词： Analeptes trifasciata Behavioural Bioassay Host Plant Volatile Cues

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Momentum as Translations at Conformal Infinity

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Journal of Applied Mathematics and Physics 2024年第4期12卷 1522-1540页

作者： richard James Petti Jacob Luke Graham Arlington MA USA Newton MA USA

Although General Relativity is the classic example of a physical theory based on differential geometry, the momentum tensor is the only part of the field equation that is not derived from or interpreted with differential geometry. This work extends General Relativity and Einstein-Cartan theory by augmenting the Poincaré group with projective (special) conformal transformations, which are translations at conformal infinity. Momentum becomes a part of the differential geometry of spacetime. The Lie algebra of these transformations is represented by vectorfields on an associated Minkowski fiber space. Variation of projective conformal scalar curvature generates a 2-index tensor that serves as linear momentum in the field equations of General Relativity. The computation yields a constructive realization of Mach’s principle: local inertia is determined by local motion relative to mass at conformal infinity in each fiber. The vectorfields have a cellular structure that is similar to that of turbulent fluids.

关键词： Projective Symmetry Conformal Symmetry Momentum General Relativity Einstein-Cartan Mach’s Principle

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Comparative utility of vestibular function tests in patients with peripheral and central vestibular dysfunction

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Journal of Otology 2024年第1期19卷 5-9页

作者： Cameron B.Fattahi Christopher Zaro Janice J.Chung richard F.Lewis Divya A.Chari Department of Otolaryngology-Head and Neck Surgery UMASS Memorial Medical CenterUniversity of Massachusetts Chan Medical SchoolWorcesterMAUSA Jenks Vestibular Physiology Laboratory Massachusetts Eye and EarBostonMAUSA Department of Otolaryngology-Head and Neck Surgery Massachusetts Eye and EarHarvard Medical SchoolBostonMAUSA Department of Neurology Harvard Medical SchoolBostonMAUSA

Background: Bithermal caloric irrigation, video head impulse test(vHIT), and rotational testing are commonly used to assess peripheral vestibular function, but the relative clinical utility of each test in differentiating patients with peripheral vestibulopathy is ***: To determine whether(1) the combination of two or more vestibular tests enhances diagnostic utility over a single test;(2) abnormal test results on vestibular tests correlate with one ***: Retrospective analysis of data collected from multidisciplinary vestibular clinics at two academic medical centers from 2016 to ***: 150 patients(54.10 ± 15.09 years, 88 females) were included. No individual test was significantly better at predicting the presence of peripheral vestibular damage(p > 0.05). vHIT test results improved significantly when combined with either the caloric test(p = 0.007) or rotary chair test(p = 0.039). Caloric and rotational testing had high sensitivity(74.65% and 76.06%, respectively) and specificity(83.54% and 78.48%, respectively). vHIT demonstrated excellent specificity(89.87%) but poor sensitivity(47.89%). Caloric, vHIT, and rotary chair tests results did not correlate with one another(p > 0.05).Conclusions: Vestibular function tests have comparable diagnostic utility, yet each offers unique advantages. Caloric and rotational testing may be best suited for screening peripheral damage and vHIT may function ideally as a confirmatory test.

关键词： vestibular patients specificity

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混凝土自复位墙结构地震全过程损伤分析

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建筑结构学报 2024年第2期45卷 189-204页

作者：鲁懿虬 HENRY richard S 周颖 RODGERS Geoffrey W 杨群顾安琪同济大学土木工程学院上海200092 奥克兰大学土木与环境系新西兰奥克兰1010 坎特伯雷大学土木与自然资源工程系新西兰基督城8140

后张法预应力混凝土自复位墙是一种有效的可恢复功能结构。虽然自复位墙自身在地震中损伤较小,但其与周边构件的变形不协调可使结构损伤严重。为控制结构层面的损伤,设计了1个框架-自复位墙结构,对自复位墙底、梁柱节点、墙梁节点、墙-... 详细信息

后张法预应力混凝土自复位墙是一种有效的可恢复功能结构。虽然自复位墙自身在地震中损伤较小,但其与周边构件的变形不协调可使结构损伤严重。为控制结构层面的损伤,设计了1个框架-自复位墙结构,对自复位墙底、梁柱节点、墙梁节点、墙-基础连接、柱-基础连接以及墙-板连接开展损伤控制设计,并对设计的结构进行足尺振动台试验。结构共经历了39次不同设计工况下不同烈度的地震激励,峰值加速度最大达1.0g,位移角最大达3.3%。对结构在历次地震作用下整体、局部构件和节点的损伤发展进行全过程定量分析,结果表明:自复位墙-开槽梁结构在地震作用下的损伤能够得到有效控制,其中多遇、设防地震作用下结构基本无损伤,罕遇地震作用下墙底损伤仅限角部剥落且面积仅为400 cm2,楼板有开裂且裂缝最大仅为1.2 mm,梁端剥落可忽略;自复位墙-开槽梁结构可使整个结构在不同设计位移角下实现多遇、设防地震不坏,罕遇地震小修甚至不修的抗震性能目标;设计的连接方式解决了结构中构件之间的变形不协调问题,提高了自复位墙结构层面的可修复性。

关键词：自复位墙开槽梁墙板连接振动台试验结构损伤

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